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The map label for this gene is hycA [H]

Identifier: 238921146

GI number: 238921146

Start: 3165962

End: 3166432

Strand: Reverse

Name: hycA [H]

Synonym: NT01EI_3287

Alternate gene names: 238921146

Gene position: 3166432-3165962 (Counterclockwise)

Preceding gene: 238921147

Following gene: 238921126

Centisome position: 83.06

GC content: 55.84

Gene sequence:

>471_bases
ATGAAGGTCAGCGAACTAACCGAGAAGTCGCTATTTATCCATAATAAGAATGAGAAGCTGATGGCGCAGTGGGCGACCTA
TAAAAGCAGCCTGATCAAGGCGACCACCTCTTCCCGGCTTAAGATTTTTCATGAGTTCAGCTGCGGTGACGGCGACAGCG
TCAACTTTACCGTGTTTGACCATTTTATGGTGGAGATCCGTCTGGCCGGGGAGTTTTACAGCAAGACCATTCAGTACGGC
TTGCGGCTGAGCGGCGGCGATGAGGATCGCTTTGTCGAGCTGGGACGGGCGACCTTTGACGATGAGGGGATCCTGGATGG
CCGGGTGAGCAACCGCGACCGCGACGGCGTGCTGGAGCACTACCTGGAAAAAATTCAGTCGCTGTATGACACTCTGTACC
ATGCCATGCAGGACAGCCGTCCGCTGCATGAGGTGCTGTTTGCCCGCTGGGGCGGTAACCTTCCCGCCTGA

Upstream 100 bases:

>100_bases
ATTTTGGTGACCGACGGGCGGTGATATCGACCGCGCGGCGGCCGCACGGCGCATCACATCGCGATGCGCGCAATCTCTGG
AGTATGGTGGAGTAATTACT

Downstream 100 bases:

>100_bases
TACCCGGGGGAAAACACCGGGCGGAGATGCCCGGTGTCGATCCGCTATCAGCAGATGCGCGGCAGCGGTTCACTGTGTGG
CAGGTCGAGCAGACGCGCCG

Product: formate hydrogenlyase regulatory protein HycA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MKVSELTEKSLFIHNKNEKLMAQWATYKSSLIKATTSSRLKIFHEFSCGDGDSVNFTVFDHFMVEIRLAGEFYSKTIQYG
LRLSGGDEDRFVELGRATFDDEGILDGRVSNRDRDGVLEHYLEKIQSLYDTLYHAMQDSRPLHEVLFARWGGNLPA

Sequences:

>Translated_156_residues
MKVSELTEKSLFIHNKNEKLMAQWATYKSSLIKATTSSRLKIFHEFSCGDGDSVNFTVFDHFMVEIRLAGEFYSKTIQYG
LRLSGGDEDRFVELGRATFDDEGILDGRVSNRDRDGVLEHYLEKIQSLYDTLYHAMQDSRPLHEVLFARWGGNLPA
>Mature_156_residues
MKVSELTEKSLFIHNKNEKLMAQWATYKSSLIKATTSSRLKIFHEFSCGDGDSVNFTVFDHFMVEIRLAGEFYSKTIQYG
LRLSGGDEDRFVELGRATFDDEGILDGRVSNRDRDGVLEHYLEKIQSLYDTLYHAMQDSRPLHEVLFARWGGNLPA

Specific function: Regulatory protein for the formate hydrogenlyase system. Could act by directly interacting with fhlA or by preventing the binding of fhlA to the upstream activatory sequence [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789080, Length=143, Percent_Identity=35.6643356643357, Blast_Score=110, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021285 [H]

Pfam domain/function: PF11046 HycA_repressor [H]

EC number: NA

Molecular weight: Translated: 17918; Mature: 17918

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVSELTEKSLFIHNKNEKLMAQWATYKSSLIKATTSSRLKIFHEFSCGDGDSVNFTVFD
CCCHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEEE
HFMVEIRLAGEFYSKTIQYGLRLSGGDEDRFVELGRATFDDEGILDGRVSNRDRDGVLEH
HEEEEEEEHHHHHHHHHHHCEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHH
YLEKIQSLYDTLYHAMQDSRPLHEVLFARWGGNLPA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKVSELTEKSLFIHNKNEKLMAQWATYKSSLIKATTSSRLKIFHEFSCGDGDSVNFTVFD
CCCHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEEE
HFMVEIRLAGEFYSKTIQYGLRLSGGDEDRFVELGRATFDDEGILDGRVSNRDRDGVLEH
HEEEEEEEHHHHHHHHHHHCEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHH
YLEKIQSLYDTLYHAMQDSRPLHEVLFARWGGNLPA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]