Click here to switch to the map view.

The map label for this gene is phoA [H]

Identifier: 238921124

GI number: 238921124

Start: 3140877

End: 3142322

Strand: Reverse

Name: phoA [H]

Synonym: NT01EI_3263

Alternate gene names: 238921124

Gene position: 3142322-3140877 (Counterclockwise)

Preceding gene: 238921125

Following gene: 238921123

Centisome position: 82.43

GC content: 60.44

Gene sequence:

>1446_bases
ATGGCGAGCCATACCCCTTTCTCCCTTTCTCTGCTGATGTTTTCTCTTGGTCTTGCCCTGCCGCTGCAGGCCGCGCCCGC
TGCGCCGAGCGCAGAGCAGGTGCTTACCGAACGCGCCGCGCACGGCGATATTACCCAGCGGGGAGGCGCCCGCCGCCTGC
TCGGCGATCAGACAGCTGCGCTGCAGGCGTCGCTGAGTACCCGCCCGGCCAAGAACGTGATCCTGCTGATCGGCGATGGC
ATGGGAGATGCGGAGATCACCGCCGCCCGTAACTATGCGGCCGGCGCGGGCGGAATGCTGCGCGGTATCGATGCCCTACC
GCTGACCGGGCAATACACGCACTATGCCCTGGATCGTAAAACCCATAAACCGGACTATGTGACGGACTCTGCGGCATCGG
CTACCGCCTGGAGCAGTGGGGTGAAAACCTATAACGGCGCATTGGGTATTGATGTAACAGGTAAACCTTATCCGACGCTG
TTGGAGATGGCGCGTGCAGCGGGCAAGGCCACCGGAAACGTCTCGACCGCAGAGCTGCAGGACGCCACGCCGGCGGCGAT
GTTCGCCCACGTCAGCGGACGCAAATGTTATGGCCCGCAGCAGACCCTGGAGAAATGTCCGGAGAATGCGTTGGAAAACG
GTGGGGCTGGCTCGATCAGCGAACAAATGTTGAAAGCGCGGGCCGATGTGACGCTGGGCGGCGGTGCAAAAACGTTTACC
CAGATAGTGGGGGCCGGTGAGTATAAGGGTAAGACGTTGCTCCAGCAGGCACAGGCGCAGGGGTACCACATCGTTAACGA
TCTGGTGGGTATGGATGCGGTGACGGTGGCAGATCAGAAGCGTCCTTTGCTGGGGCTGTTCGCCGAGGGCAATATGGCGG
TGCGTTGGCTGGGGCCAAAAGCCAGTCTGCACGGTAACCTGGAGCAGGCGCCGGTCACCTGCCAGCCGAATCCGCAGCGC
AGTGACAGCCAGCCGACGCTGGCACAGATGACCGGCAAAGCCATTTCGCTTTTGAGCAAGAATCCAAATGGTTTCTTCCT
GCAGGTCGAAGGTGCTTCTATCGATAAACAGGATCATGCCGCCAATCCGTGCGGTCAGTTTGGTGAGACGGTCGATTTGG
ATGAGGCGGTTCAAAAGGCGCTGGAGTTTGCCCGCCGTGACGGGAATACCCTGGTAGTGGTAACGGCGGATCATGCTCAT
AGCAGCCAGATTATCCCCAACGATGCCAAGGCGCCGGGGCTGACTCAGACGCTTATCACCCAGGATGGGGTACCGATGAC
CATTAGCTACGGCACCGCTGAGGAGGCGGACAGCCAGGAGCATACCGGCTCCCAGATACGTATCGCTGCGTATGGTCCTC
ATGCCGGTAACGTAGTGGGGCTGACGGATCAGACGGATCTGTTCTTTACCCTGCGCGATGCGATGGGACTTAACGCCGCG
AAATAA

Upstream 100 bases:

>100_bases
GTTCACGGGATCGTCACCGAACCGACATTATCTCTCCTTATACTCTGTTCCAACTTATTCATTAGTTGAATGATTTTCCT
CTTGGACACGGAGTCTTATC

Downstream 100 bases:

>100_bases
CGGCATAACAGCAGTATCGGCCCGGTGCGGCGTAGTGGCGGCAGCGGGCCGTTTTTTATTGCGTTAAAGTATCAGTGAAC
TATTTGTTGTTGTTGTGTTA

Product: alkaline phosphatase

Products: NA

Alternate protein names: APase [H]

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MASHTPFSLSLLMFSLGLALPLQAAPAAPSAEQVLTERAAHGDITQRGGARRLLGDQTAALQASLSTRPAKNVILLIGDG
MGDAEITAARNYAAGAGGMLRGIDALPLTGQYTHYALDRKTHKPDYVTDSAASATAWSSGVKTYNGALGIDVTGKPYPTL
LEMARAAGKATGNVSTAELQDATPAAMFAHVSGRKCYGPQQTLEKCPENALENGGAGSISEQMLKARADVTLGGGAKTFT
QIVGAGEYKGKTLLQQAQAQGYHIVNDLVGMDAVTVADQKRPLLGLFAEGNMAVRWLGPKASLHGNLEQAPVTCQPNPQR
SDSQPTLAQMTGKAISLLSKNPNGFFLQVEGASIDKQDHAANPCGQFGETVDLDEAVQKALEFARRDGNTLVVVTADHAH
SSQIIPNDAKAPGLTQTLITQDGVPMTISYGTAEEADSQEHTGSQIRIAAYGPHAGNVVGLTDQTDLFFTLRDAMGLNAA
K

Sequences:

>Translated_481_residues
MASHTPFSLSLLMFSLGLALPLQAAPAAPSAEQVLTERAAHGDITQRGGARRLLGDQTAALQASLSTRPAKNVILLIGDG
MGDAEITAARNYAAGAGGMLRGIDALPLTGQYTHYALDRKTHKPDYVTDSAASATAWSSGVKTYNGALGIDVTGKPYPTL
LEMARAAGKATGNVSTAELQDATPAAMFAHVSGRKCYGPQQTLEKCPENALENGGAGSISEQMLKARADVTLGGGAKTFT
QIVGAGEYKGKTLLQQAQAQGYHIVNDLVGMDAVTVADQKRPLLGLFAEGNMAVRWLGPKASLHGNLEQAPVTCQPNPQR
SDSQPTLAQMTGKAISLLSKNPNGFFLQVEGASIDKQDHAANPCGQFGETVDLDEAVQKALEFARRDGNTLVVVTADHAH
SSQIIPNDAKAPGLTQTLITQDGVPMTISYGTAEEADSQEHTGSQIRIAAYGPHAGNVVGLTDQTDLFFTLRDAMGLNAA
K
>Mature_480_residues
ASHTPFSLSLLMFSLGLALPLQAAPAAPSAEQVLTERAAHGDITQRGGARRLLGDQTAALQASLSTRPAKNVILLIGDGM
GDAEITAARNYAAGAGGMLRGIDALPLTGQYTHYALDRKTHKPDYVTDSAASATAWSSGVKTYNGALGIDVTGKPYPTLL
EMARAAGKATGNVSTAELQDATPAAMFAHVSGRKCYGPQQTLEKCPENALENGGAGSISEQMLKARADVTLGGGAKTFTQ
IVGAGEYKGKTLLQQAQAQGYHIVNDLVGMDAVTVADQKRPLLGLFAEGNMAVRWLGPKASLHGNLEQAPVTCQPNPQRS
DSQPTLAQMTGKAISLLSKNPNGFFLQVEGASIDKQDHAANPCGQFGETVDLDEAVQKALEFARRDGNTLVVVTADHAHS
SQIIPNDAKAPGLTQTLITQDGVPMTISYGTAEEADSQEHTGSQIRIAAYGPHAGNVVGLTDQTDLFFTLRDAMGLNAAK

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI94721246, Length=480, Percent_Identity=27.9166666666667, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI157266296, Length=481, Percent_Identity=28.4823284823285, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI116734717, Length=395, Percent_Identity=31.1392405063291, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI157266292, Length=374, Percent_Identity=29.6791443850267, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI294660770, Length=385, Percent_Identity=30.1298701298701, Blast_Score=114, Evalue=3e-25,
Organism=Escherichia coli, GI48994877, Length=444, Percent_Identity=75.4504504504504, Blast_Score=697, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320689, Length=365, Percent_Identity=31.7808219178082, Blast_Score=130, Evalue=3e-31,
Organism=Drosophila melanogaster, GI19921912, Length=473, Percent_Identity=29.3868921775898, Blast_Score=149, Evalue=5e-36,
Organism=Drosophila melanogaster, GI21355151, Length=477, Percent_Identity=28.9308176100629, Blast_Score=145, Evalue=6e-35,
Organism=Drosophila melanogaster, GI21355981, Length=425, Percent_Identity=29.4117647058824, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI18859923, Length=349, Percent_Identity=31.8051575931232, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI21358067, Length=532, Percent_Identity=27.2556390977444, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24585213, Length=388, Percent_Identity=29.639175257732, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI21355149, Length=506, Percent_Identity=25.8893280632411, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24651554, Length=495, Percent_Identity=27.6767676767677, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI85725200, Length=416, Percent_Identity=26.6826923076923, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI85815833, Length=416, Percent_Identity=26.6826923076923, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24657827, Length=369, Percent_Identity=31.4363143631436, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24657835, Length=354, Percent_Identity=27.9661016949153, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24657842, Length=363, Percent_Identity=25.8953168044077, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 50284; Mature: 50153

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00123 ALKALINE_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASHTPFSLSLLMFSLGLALPLQAAPAAPSAEQVLTERAAHGDITQRGGARRLLGDQTAA
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCHHHCCCCHHHHCCCHHH
LQASLSTRPAKNVILLIGDGMGDAEITAARNYAAGAGGMLRGIDALPLTGQYTHYALDRK
HHHHCCCCCCCCEEEEEECCCCCCEEEECCCCCCCCCCHHHCCCCCCCCCCCCEEEECCC
THKPDYVTDSAASATAWSSGVKTYNGALGIDVTGKPYPTLLEMARAAGKATGNVSTAELQ
CCCCCCCCCCCCCHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCHHHHC
DATPAAMFAHVSGRKCYGPQQTLEKCPENALENGGAGSISEQMLKARADVTLGGGAKTFT
CCCCHHHEEECCCCCCCCHHHHHHHCHHHHHHCCCCCCHHHHHHHHHCCEEECCCHHHHH
QIVGAGEYKGKTLLQQAQAQGYHIVNDLVGMDAVTVADQKRPLLGLFAEGNMAVRWLGPK
HHHCCCCCCCHHHHHHHHHCCCEEHHHHHCCCEEEECCCCCCEEEEEECCCEEEEEECCC
ASLHGNLEQAPVTCQPNPQRSDSQPTLAQMTGKAISLLSKNPNGFFLQVEGASIDKQDHA
CCCCCCCCCCCEECCCCCCCCCCCCHHHHHCCHHHHHHCCCCCEEEEEEECCCCCCHHCC
ANPCGQFGETVDLDEAVQKALEFARRDGNTLVVVTADHAHSSQIIPNDAKAPGLTQTLIT
CCCHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHC
QDGVPMTISYGTAEEADSQEHTGSQIRIAAYGPHAGNVVGLTDQTDLFFTLRDAMGLNAA
CCCCCEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCEEEECCCCCEEEEEHHHCCCCCC
K
C
>Mature Secondary Structure 
ASHTPFSLSLLMFSLGLALPLQAAPAAPSAEQVLTERAAHGDITQRGGARRLLGDQTAA
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCHHHCCCCHHHHCCCHHH
LQASLSTRPAKNVILLIGDGMGDAEITAARNYAAGAGGMLRGIDALPLTGQYTHYALDRK
HHHHCCCCCCCCEEEEEECCCCCCEEEECCCCCCCCCCHHHCCCCCCCCCCCCEEEECCC
THKPDYVTDSAASATAWSSGVKTYNGALGIDVTGKPYPTLLEMARAAGKATGNVSTAELQ
CCCCCCCCCCCCCHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCHHHHC
DATPAAMFAHVSGRKCYGPQQTLEKCPENALENGGAGSISEQMLKARADVTLGGGAKTFT
CCCCHHHEEECCCCCCCCHHHHHHHCHHHHHHCCCCCCHHHHHHHHHCCEEECCCHHHHH
QIVGAGEYKGKTLLQQAQAQGYHIVNDLVGMDAVTVADQKRPLLGLFAEGNMAVRWLGPK
HHHCCCCCCCHHHHHHHHHCCCEEHHHHHCCCEEEECCCCCCEEEEEECCCEEEEEECCC
ASLHGNLEQAPVTCQPNPQRSDSQPTLAQMTGKAISLLSKNPNGFFLQVEGASIDKQDHA
CCCCCCCCCCCEECCCCCCCCCCCCHHHHHCCHHHHHHCCCCCEEEEEEECCCCCCHHCC
ANPCGQFGETVDLDEAVQKALEFARRDGNTLVVVTADHAHSSQIIPNDAKAPGLTQTLIT
CCCHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHC
QDGVPMTISYGTAEEADSQEHTGSQIRIAAYGPHAGNVVGLTDQTDLFFTLRDAMGLNAA
CCCCCEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCEEEECCCCCEEEEEHHHCCCCCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2129542 [H]