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The map label for this gene is ptsP [H]

Identifier: 238921119

GI number: 238921119

Start: 3134852

End: 3137098

Strand: Reverse

Name: ptsP [H]

Synonym: NT01EI_3258

Alternate gene names: 238921119

Gene position: 3137098-3134852 (Counterclockwise)

Preceding gene: 238921120

Following gene: 238921118

Centisome position: 82.29

GC content: 63.42

Gene sequence:

>2247_bases
ATGCTTACACGCCTGCGCGAGATCGTGGAAAAGGTGGCTGCGGCTGCCAGCCTGAATGACGCGCTGAATGTGCTGGTGGA
CGAAACCTGTCGGGCGATGGAGACGGAGGTTTGCTCTGTTTACCTGGCCGACAACGATCGCCAGTGCTACTACCTGATGG
CGACCCGTGGTCTGCAAAAGCCGCGTGGTCGCGTGGTGGCGCTCTCTTTCGACGAAGGGATCGTCGGCCTGGTGGGTCGT
CTGGCGGAGCCGGTGAACCTGGCGGATGCGCCCAGCCATCCCAGCTTTAAATATCTGCCGCAGGTGCGCGAGGAGTGTTA
CCGTGCATTCCTGGGCGTGCCGATGATCTATCGCCGTCAGCTGCTGGGGGTGCTGGTCATACAGCAGCGTGCGTCCCGTC
AGTTTGATGAGGTTGAAGAGTCCTTTCTGGTAACCCTGGCGACTCAGCTGGCCGGGATCCTGTCGCAAACCCAGCTCAAC
ACCCTGTTTGGCCGCCTGCGCCAGAATCGCCTGCGCGCTATCGCCGCGGCGCCAGGTGTCGCAGTGGCCAAGGGATGGAT
GGACATCAGCCAACCGGCGCTGGAGCATGTTTATGAAGCATCTACTCTGGATACCGACAGCGAGCGTGAGCGACTGGCGC
GGGCGCTGGAGGAGGCAGCCAGCGAGTTTCGCCGCTATAGCAAACGCTTTGGCGCCAGTGCCCAGAAAGAGAGCGCGGCG
ATCTTCGATCTCTACTCTCACCTGCTCAACGATCCGCGTCTGAAGCGTGAGCTGATGCATCACGTCGACGGCGGCGCGGT
GGCCGAGTGGGCTGTGAAGCAGGTCACCGAGCACTTTGCCGACCAGTTTGCCAGTCTGCAGGACAGCTATATGCGCGATC
GCGCCAGCGATTTACGCGCGCTCGGGCAGCGTCTGTTGTTCCATCTGGATGATAACGCCATCGGTCCGGCGCCGTGGCCG
GAGCGCTTTATTCTGGTGGCCGATGAGCTGACGCCGACCCTGCTGGCGGAGATCCCGCAGTCCTGTCTGCTGGGGGTGGT
GGTGCGTGACGGTGCCTCTAACTCCCACGCGGCAATTATCCTGCGCGCGATGGGCGTACCGACGGTGATGGGGGCCGATA
TTCGCCCTGCGCTGCTCCATGAGCGGCTGCTGATTATTGATGGCTACCGCGGAGAGGTGCTGGTCGAGCCGGAGCCGGTT
TTGGTGCAGGAATACCAGCGGCTGATCAGCGAAGAGCAGGCGCTGAGCCGTAAGACGGAGGCCGAGTCGGATCGCCCGGC
GCAGCTGCGCAGTGGCGAACGGATCGTTGTGCTGCTGAACGCCGGCCTGAGCGCAGAGCATGAGCAGCGTCTGGAGGCTT
GCGTCGACGGCGTGGGGCTATACCGTACCGAAATTCCTTTTATGTTGCAGAGCGGCTTTCCGTCCGAGGAGGAGCAGGTT
GCACAGTATCAGGGTATGCTGCAGATAAACCCGGCGAAGCCGGTAGTGCTGCGCACCCTGGATATCGGTGCCGACAAGCA
GCTACCCTACATGCCGATTAGCGAGGAGAACCCCAGCCTGGGCTGGCGTGGGCTACGGATCACCCTCGATCAGCCGGAGA
TCTTTCTGGTGCAGGTGCGCGCCATGCTGCGCGCCAATGCGGCCAGCGGCAACCTGGGGATCCTGCTGCCGATGGTGACA
ACCCTGGCCGAAGTGGATGAAGCGCGTCGCCTGATCGAGCGCGCCGGACGCGAGGTTGAGGAGATGTTGGGCTATGCCCT
GCCGCGCCCGCGTATCGGGGTGATGCTGGAGGTGCCGGCGTTGGCCTTTATGCTGTCGGGGCTGGCCTCACGGGTCGACT
TTATTTCGGTCGGCACCAACGATTTGACCCAGTATCTGCTGGCGGTGGATCGCAACAATACCCGGGTGGCCGCGCTGTAT
GATGGCCTGCACCCGGCGATGCTGCGCCTGCTGCAGCAGGTGGTGCGGGAGTGTGCCGAGCTGGGGCTGCCGGTCAGCGT
CTGCGGCGAGATGGCCGGCGATCCGATGGGCGCACTGCTGCTGGTGGGGCTGGGGTTCCGCCAGCTGAGCATGAACGGGC
ACAGTGTGCCGCGTATCAAGTATCTGCTGCGTCACCTGGATCTGCAGGATATCGAGGGGCTGGCCGCCCGCATGGTGGCG
GCCCATTCATCGACCGAGGTGCGTCACCTCACCGCCTCGTTTTTAGAGCGGCGCGGCGTCGGTGGTCTGATCCGTGGCGG
AATGTAG

Upstream 100 bases:

>100_bases
GGAGTTCTCATCCGTTGTCATGTCGTTGCAGGAGAGCGTAGCCCAGGGAGGGCGTTCGGCTCCCGGCTATCGCCGTAAAA
GAGGTTAAGTGGCAGTAATC

Downstream 100 bases:

>100_bases
GGCACCTCCGGGGAATTTTGCCGTCATGATGGAGTCCTGACGGAAGTTATGGGATGATTGGGCGGTTTTTCAGGGGGCGT
GAATGGGTAAACTGACGCTA

Product: fused phosphoenolpyruvate-protein phosphotransferase PtsP/GAF domain

Products: NA

Alternate protein names: Enzyme I-Ntr; Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 748; Mature: 748

Protein sequence:

>748_residues
MLTRLREIVEKVAAAASLNDALNVLVDETCRAMETEVCSVYLADNDRQCYYLMATRGLQKPRGRVVALSFDEGIVGLVGR
LAEPVNLADAPSHPSFKYLPQVREECYRAFLGVPMIYRRQLLGVLVIQQRASRQFDEVEESFLVTLATQLAGILSQTQLN
TLFGRLRQNRLRAIAAAPGVAVAKGWMDISQPALEHVYEASTLDTDSERERLARALEEAASEFRRYSKRFGASAQKESAA
IFDLYSHLLNDPRLKRELMHHVDGGAVAEWAVKQVTEHFADQFASLQDSYMRDRASDLRALGQRLLFHLDDNAIGPAPWP
ERFILVADELTPTLLAEIPQSCLLGVVVRDGASNSHAAIILRAMGVPTVMGADIRPALLHERLLIIDGYRGEVLVEPEPV
LVQEYQRLISEEQALSRKTEAESDRPAQLRSGERIVVLLNAGLSAEHEQRLEACVDGVGLYRTEIPFMLQSGFPSEEEQV
AQYQGMLQINPAKPVVLRTLDIGADKQLPYMPISEENPSLGWRGLRITLDQPEIFLVQVRAMLRANAASGNLGILLPMVT
TLAEVDEARRLIERAGREVEEMLGYALPRPRIGVMLEVPALAFMLSGLASRVDFISVGTNDLTQYLLAVDRNNTRVAALY
DGLHPAMLRLLQQVVRECAELGLPVSVCGEMAGDPMGALLLVGLGFRQLSMNGHSVPRIKYLLRHLDLQDIEGLAARMVA
AHSSTEVRHLTASFLERRGVGGLIRGGM

Sequences:

>Translated_748_residues
MLTRLREIVEKVAAAASLNDALNVLVDETCRAMETEVCSVYLADNDRQCYYLMATRGLQKPRGRVVALSFDEGIVGLVGR
LAEPVNLADAPSHPSFKYLPQVREECYRAFLGVPMIYRRQLLGVLVIQQRASRQFDEVEESFLVTLATQLAGILSQTQLN
TLFGRLRQNRLRAIAAAPGVAVAKGWMDISQPALEHVYEASTLDTDSERERLARALEEAASEFRRYSKRFGASAQKESAA
IFDLYSHLLNDPRLKRELMHHVDGGAVAEWAVKQVTEHFADQFASLQDSYMRDRASDLRALGQRLLFHLDDNAIGPAPWP
ERFILVADELTPTLLAEIPQSCLLGVVVRDGASNSHAAIILRAMGVPTVMGADIRPALLHERLLIIDGYRGEVLVEPEPV
LVQEYQRLISEEQALSRKTEAESDRPAQLRSGERIVVLLNAGLSAEHEQRLEACVDGVGLYRTEIPFMLQSGFPSEEEQV
AQYQGMLQINPAKPVVLRTLDIGADKQLPYMPISEENPSLGWRGLRITLDQPEIFLVQVRAMLRANAASGNLGILLPMVT
TLAEVDEARRLIERAGREVEEMLGYALPRPRIGVMLEVPALAFMLSGLASRVDFISVGTNDLTQYLLAVDRNNTRVAALY
DGLHPAMLRLLQQVVRECAELGLPVSVCGEMAGDPMGALLLVGLGFRQLSMNGHSVPRIKYLLRHLDLQDIEGLAARMVA
AHSSTEVRHLTASFLERRGVGGLIRGGM
>Mature_748_residues
MLTRLREIVEKVAAAASLNDALNVLVDETCRAMETEVCSVYLADNDRQCYYLMATRGLQKPRGRVVALSFDEGIVGLVGR
LAEPVNLADAPSHPSFKYLPQVREECYRAFLGVPMIYRRQLLGVLVIQQRASRQFDEVEESFLVTLATQLAGILSQTQLN
TLFGRLRQNRLRAIAAAPGVAVAKGWMDISQPALEHVYEASTLDTDSERERLARALEEAASEFRRYSKRFGASAQKESAA
IFDLYSHLLNDPRLKRELMHHVDGGAVAEWAVKQVTEHFADQFASLQDSYMRDRASDLRALGQRLLFHLDDNAIGPAPWP
ERFILVADELTPTLLAEIPQSCLLGVVVRDGASNSHAAIILRAMGVPTVMGADIRPALLHERLLIIDGYRGEVLVEPEPV
LVQEYQRLISEEQALSRKTEAESDRPAQLRSGERIVVLLNAGLSAEHEQRLEACVDGVGLYRTEIPFMLQSGFPSEEEQV
AQYQGMLQINPAKPVVLRTLDIGADKQLPYMPISEENPSLGWRGLRITLDQPEIFLVQVRAMLRANAASGNLGILLPMVT
TLAEVDEARRLIERAGREVEEMLGYALPRPRIGVMLEVPALAFMLSGLASRVDFISVGTNDLTQYLLAVDRNNTRVAALY
DGLHPAMLRLLQQVVRECAELGLPVSVCGEMAGDPMGALLLVGLGFRQLSMNGHSVPRIKYLLRHLDLQDIEGLAARMVA
AHSSTEVRHLTASFLERRGVGGLIRGGM

Specific function: Component of the phosphoenolpyruvate-dependent nitrogen- metabolic phosphotransferase system (nitrogen-metabolic PTS), that seems to be involved in regulating nitrogen metabolism. Enzyme I- Ntr transfers the phosphoryl group from phosphoenolpyruvate (PEP)

COG id: COG3605

COG function: function code T; Signal transduction protein containing GAF and PtsI domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GAF domain [H]

Homologues:

Organism=Escherichia coli, GI1789193, Length=748, Percent_Identity=73.5294117647059, Blast_Score=1135, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=577, Percent_Identity=35.0086655112652, Blast_Score=313, Evalue=4e-86,
Organism=Escherichia coli, GI48994992, Length=501, Percent_Identity=32.7345309381238, Blast_Score=265, Evalue=1e-71,
Organism=Escherichia coli, GI1788726, Length=524, Percent_Identity=31.8702290076336, Blast_Score=234, Evalue=2e-62,
Organism=Escherichia coli, GI226510935, Length=179, Percent_Identity=29.608938547486, Blast_Score=79, Evalue=2e-15,
Organism=Escherichia coli, GI1787994, Length=419, Percent_Identity=27.2076372315036, Blast_Score=77, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003018
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF01590 GAF; PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 82796; Mature: 82796

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRLREIVEKVAAAASLNDALNVLVDETCRAMETEVCSVYLADNDRQCYYLMATRGLQK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEEECCCCCC
PRGRVVALSFDEGIVGLVGRLAEPVNLADAPSHPSFKYLPQVREECYRAFLGVPMIYRRQ
CCCCEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
LLGVLVIQQRASRQFDEVEESFLVTLATQLAGILSQTQLNTLFGRLRQNRLRAIAAAPGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
AVAKGWMDISQPALEHVYEASTLDTDSERERLARALEEAASEFRRYSKRFGASAQKESAA
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
IFDLYSHLLNDPRLKRELMHHVDGGAVAEWAVKQVTEHFADQFASLQDSYMRDRASDLRA
HHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGQRLLFHLDDNAIGPAPWPERFILVADELTPTLLAEIPQSCLLGVVVRDGASNSHAAII
HHHHHEEEECCCCCCCCCCCCCEEEEECCCCHHHHHHCCHHHHHHHHEECCCCCCCCEEE
LRAMGVPTVMGADIRPALLHERLLIIDGYRGEVLVEPEPVLVQEYQRLISEEQALSRKTE
EEECCCCCCCCCCCCHHHHHCCEEEEECCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHC
AESDRPAQLRSGERIVVLLNAGLSAEHEQRLEACVDGVGLYRTEIPFMLQSGFPSEEEQV
CCCCCCHHHCCCCEEEEEEECCCCCHHHHHHHHHHCCCCHHHHCCCHHHHCCCCCHHHHH
AQYQGMLQINPAKPVVLRTLDIGADKQLPYMPISEENPSLGWRGLRITLDQPEIFLVQVR
HHHCCCEEECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHH
AMLRANAASGNLGILLPMVTTLAEVDEARRLIERAGREVEEMLGYALPRPRIGVMLEVPA
HHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCEEEEHHH
LAFMLSGLASRVDFISVGTNDLTQYLLAVDRNNTRVAALYDGLHPAMLRLLQQVVRECAE
HHHHHHHHHHHHHEEECCHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHH
LGLPVSVCGEMAGDPMGALLLVGLGFRQLSMNGHSVPRIKYLLRHLDLQDIEGLAARMVA
CCCCHHHHHHHCCCCHHHHHHHHCCHHEECCCCCCCHHHHHHHHHCCHHHHHHHHHHHHH
AHSSTEVRHLTASFLERRGVGGLIRGGM
HCCCCHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLTRLREIVEKVAAAASLNDALNVLVDETCRAMETEVCSVYLADNDRQCYYLMATRGLQK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEEECCCCCC
PRGRVVALSFDEGIVGLVGRLAEPVNLADAPSHPSFKYLPQVREECYRAFLGVPMIYRRQ
CCCCEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
LLGVLVIQQRASRQFDEVEESFLVTLATQLAGILSQTQLNTLFGRLRQNRLRAIAAAPGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
AVAKGWMDISQPALEHVYEASTLDTDSERERLARALEEAASEFRRYSKRFGASAQKESAA
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
IFDLYSHLLNDPRLKRELMHHVDGGAVAEWAVKQVTEHFADQFASLQDSYMRDRASDLRA
HHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGQRLLFHLDDNAIGPAPWPERFILVADELTPTLLAEIPQSCLLGVVVRDGASNSHAAII
HHHHHEEEECCCCCCCCCCCCCEEEEECCCCHHHHHHCCHHHHHHHHEECCCCCCCCEEE
LRAMGVPTVMGADIRPALLHERLLIIDGYRGEVLVEPEPVLVQEYQRLISEEQALSRKTE
EEECCCCCCCCCCCCHHHHHCCEEEEECCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHC
AESDRPAQLRSGERIVVLLNAGLSAEHEQRLEACVDGVGLYRTEIPFMLQSGFPSEEEQV
CCCCCCHHHCCCCEEEEEEECCCCCHHHHHHHHHHCCCCHHHHCCCHHHHCCCCCHHHHH
AQYQGMLQINPAKPVVLRTLDIGADKQLPYMPISEENPSLGWRGLRITLDQPEIFLVQVR
HHHCCCEEECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHH
AMLRANAASGNLGILLPMVTTLAEVDEARRLIERAGREVEEMLGYALPRPRIGVMLEVPA
HHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCEEEEHHH
LAFMLSGLASRVDFISVGTNDLTQYLLAVDRNNTRVAALYDGLHPAMLRLLQQVVRECAE
HHHHHHHHHHHHHEEECCHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHH
LGLPVSVCGEMAGDPMGALLLVGLGFRQLSMNGHSVPRIKYLLRHLDLQDIEGLAARMVA
CCCCHHHHHHHCCCCHHHHHHHHCCHHEECCCCCCCHHHHHHHHHCCHHHHHHHHHHHHH
AHSSTEVRHLTASFLERRGVGGLIRGGM
HCCCCHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 7896715; 8973315 [H]