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The map label for this gene is miaB [H]

Identifier: 238920797

GI number: 238920797

Start: 2811124

End: 2812548

Strand: Direct

Name: miaB [H]

Synonym: NT01EI_2923

Alternate gene names: 238920797

Gene position: 2811124-2812548 (Clockwise)

Preceding gene: 238920795

Following gene: 238920799

Centisome position: 73.74

GC content: 61.19

Gene sequence:

>1425_bases
ATGACAAAAAAACTGCATATTAAAACCTGGGGCTGTCAGATGAATGAGTATGACTCATCCAAGATAGCCGATCTGCTGGG
CAGCACGCATGGCCTTGAGCTGACGGATAATGCCGAGGACGCCGATGTCCTGCTGCTGAATACCTGTTCGATCCGTGAAA
AGGCGCAGGAAAAAGTGTTCCATCAGCTTGGCCGCTGGAAGATGCTCAAAGAGGCCAAGCCGGATCTGATCATCGGCGTC
GGCGGCTGCGTCGCCTCCCAGGAGGGTGAGCATATCCGCGAGCGCGCCAGCTTTGTCGACATCATCTTTGGCCCGCAGAC
ACTGCACCGCCTGCCGGAGATGATCGACAGCGCGCGCAGCAGCCACGGCCCGGTGGTTGACGTCAGCTTCCCGGAGATCG
AAAAGTTCGACCGCCTGCCGGAGCCGCGCGCCGACGGCGCCACCGCCTTTGTCTCCATCATGGAGGGCTGCAACAAATAC
TGTACCTACTGCGTGGTGCCCTACACCCGCGGCGCCGAGGTCAGCCGCCCGTGCGACGACGTGCTGTTTGAAATCGCCCA
GCTGGCCGCCCAGGGCGTACGCGAGGTCAACCTGCTGGGGCAGAACGTCAACGCCTACCGCGGTGCCACCTTCGATGGCG
GTATCTGCTCCTTTGCCGAGCTGCTGCGCCTGGTGGCCGCCATTGACGGCATCGACCGTATCCGCTTCACCACCAGCCAT
CCAATAGAGTTCAGCGACGACATCGTGGCCGTCTATGAGGATACGCCGGAGCTGGTCAGCTTCCTGCACCTGCCGGTGCA
GAGCGGATCCGACCGCATCCTCAACCTGATGAAGCGCACCCATACCGCGCTGGAGTACAAGGCGATCATCCGTAAACTGC
GCAGGGCGCGCCCGGATATTCAGATAAGCTCCGACTTTATCGTCGGCTTCCCCGGTGAGACCCAGCAGGACTTCGAACAG
ACCATGCAGCTGATCGCCGAGGTGGACTTCGACATGAGCTACAGCTTTATCTTTTCCGCCCGCCCGGGTACCCCGGCTGC
CGATATGCTGGACGACGTGGCGGAAGAGGAGAAAAAGCAGCGTCTGTACATTCTGCAGGAGCGTATCAACCAGCAGGCCA
TGCGCTACAGCCGCCACATGCTGGGCACGGTACAGCGCATTCTGGTCGAGGGCACCTCGCGCAAAAACGTGATGGAGCTT
TCCGGCCGCACCGAAAACAACCGGGTGGTCAACTTTGCCGGCCAGCCGGAGATGATCGGGCGCTTCGTCGACGTTGAGAT
CACCGGGGTACTGCCCAACTCCCTGCGCGGCGTGGTGGTACGCAGTGAAGATGAGATGGGGCTACGCATCAGCGAGTCGC
CGCAGTCGGTGATCGCCCGCACCCGCAAAGAGGACGATCTGGGCGTCGGCAGCTACCAGCCGTAA

Upstream 100 bases:

>100_bases
ACAATAGCCGCCCTGCCGCGGACGCAACGGTCACCGCCGGCACGGCGCCTGAGGGCGTCTGCGCGTCCCTGATCCACCTG
CACTGAGCAATGGCAAGTCG

Downstream 100 bases:

>100_bases
ACCGCCCGCATCACGACGCCCGGTCCAGGCCGGGCGTTTTATTTGATGCGCTTAGCCCGCCGCGGGATAGACATACCCCC
CCTGAACGCCCAACGGTATC

Product: tRNA-I(6)

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MTKKLHIKTWGCQMNEYDSSKIADLLGSTHGLELTDNAEDADVLLLNTCSIREKAQEKVFHQLGRWKMLKEAKPDLIIGV
GGCVASQEGEHIRERASFVDIIFGPQTLHRLPEMIDSARSSHGPVVDVSFPEIEKFDRLPEPRADGATAFVSIMEGCNKY
CTYCVVPYTRGAEVSRPCDDVLFEIAQLAAQGVREVNLLGQNVNAYRGATFDGGICSFAELLRLVAAIDGIDRIRFTTSH
PIEFSDDIVAVYEDTPELVSFLHLPVQSGSDRILNLMKRTHTALEYKAIIRKLRRARPDIQISSDFIVGFPGETQQDFEQ
TMQLIAEVDFDMSYSFIFSARPGTPAADMLDDVAEEEKKQRLYILQERINQQAMRYSRHMLGTVQRILVEGTSRKNVMEL
SGRTENNRVVNFAGQPEMIGRFVDVEITGVLPNSLRGVVVRSEDEMGLRISESPQSVIARTRKEDDLGVGSYQP

Sequences:

>Translated_474_residues
MTKKLHIKTWGCQMNEYDSSKIADLLGSTHGLELTDNAEDADVLLLNTCSIREKAQEKVFHQLGRWKMLKEAKPDLIIGV
GGCVASQEGEHIRERASFVDIIFGPQTLHRLPEMIDSARSSHGPVVDVSFPEIEKFDRLPEPRADGATAFVSIMEGCNKY
CTYCVVPYTRGAEVSRPCDDVLFEIAQLAAQGVREVNLLGQNVNAYRGATFDGGICSFAELLRLVAAIDGIDRIRFTTSH
PIEFSDDIVAVYEDTPELVSFLHLPVQSGSDRILNLMKRTHTALEYKAIIRKLRRARPDIQISSDFIVGFPGETQQDFEQ
TMQLIAEVDFDMSYSFIFSARPGTPAADMLDDVAEEEKKQRLYILQERINQQAMRYSRHMLGTVQRILVEGTSRKNVMEL
SGRTENNRVVNFAGQPEMIGRFVDVEITGVLPNSLRGVVVRSEDEMGLRISESPQSVIARTRKEDDLGVGSYQP
>Mature_473_residues
TKKLHIKTWGCQMNEYDSSKIADLLGSTHGLELTDNAEDADVLLLNTCSIREKAQEKVFHQLGRWKMLKEAKPDLIIGVG
GCVASQEGEHIRERASFVDIIFGPQTLHRLPEMIDSARSSHGPVVDVSFPEIEKFDRLPEPRADGATAFVSIMEGCNKYC
TYCVVPYTRGAEVSRPCDDVLFEIAQLAAQGVREVNLLGQNVNAYRGATFDGGICSFAELLRLVAAIDGIDRIRFTTSHP
IEFSDDIVAVYEDTPELVSFLHLPVQSGSDRILNLMKRTHTALEYKAIIRKLRRARPDIQISSDFIVGFPGETQQDFEQT
MQLIAEVDFDMSYSFIFSARPGTPAADMLDDVAEEEKKQRLYILQERINQQAMRYSRHMLGTVQRILVEGTSRKNVMELS
GRTENNRVVNFAGQPEMIGRFVDVEITGVLPNSLRGVVVRSEDEMGLRISESPQSVIARTRKEDDLGVGSYQP

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=482, Percent_Identity=37.1369294605809, Blast_Score=289, Evalue=3e-78,
Organism=Homo sapiens, GI28872782, Length=482, Percent_Identity=37.1369294605809, Blast_Score=289, Evalue=4e-78,
Organism=Homo sapiens, GI93277076, Length=437, Percent_Identity=30.2059496567506, Blast_Score=178, Evalue=1e-44,
Organism=Escherichia coli, GI1786882, Length=474, Percent_Identity=85.8649789029536, Blast_Score=858, Evalue=0.0,
Organism=Escherichia coli, GI1787057, Length=460, Percent_Identity=28.4782608695652, Blast_Score=155, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17553146, Length=465, Percent_Identity=33.5483870967742, Blast_Score=246, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI71996771, Length=301, Percent_Identity=29.2358803986711, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI21356207, Length=433, Percent_Identity=35.3348729792148, Blast_Score=266, Evalue=3e-71,
Organism=Drosophila melanogaster, GI19922432, Length=454, Percent_Identity=29.0748898678414, Blast_Score=157, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 53125; Mature: 52994

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKLHIKTWGCQMNEYDSSKIADLLGSTHGLELTDNAEDADVLLLNTCSIREKAQEKVF
CCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCEECCCCCCCCEEEEECHHHHHHHHHHHH
HQLGRWKMLKEAKPDLIIGVGGCVASQEGEHIRERASFVDIIFGPQTLHRLPEMIDSARS
HHHHHHHHHHHCCCCEEEECCCHHCCCCCHHHHHHHHHEEEEECHHHHHHHHHHHHHHHC
SHGPVVDVSFPEIEKFDRLPEPRADGATAFVSIMEGCNKYCTYCVVPYTRGAEVSRPCDD
CCCCEEECCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHH
VLFEIAQLAAQGVREVNLLGQNVNAYRGATFDGGICSFAELLRLVAAIDGIDRIRFTTSH
HHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHHHHCCCHHEEEECCC
PIEFSDDIVAVYEDTPELVSFLHLPVQSGSDRILNLMKRTHTALEYKAIIRKLRRARPDI
CCCCCCCEEEEECCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
QISSDFIVGFPGETQQDFEQTMQLIAEVDFDMSYSFIFSARPGTPAADMLDDVAEEEKKQ
EECCCEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHH
RLYILQERINQQAMRYSRHMLGTVQRILVEGTSRKNVMELSGRTENNRVVNFAGQPEMIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCEEEEECCCHHHHC
RFVDVEITGVLPNSLRGVVVRSEDEMGLRISESPQSVIARTRKEDDLGVGSYQP
EEEEEEEEEECCCCCCEEEEECCCCCCCEECCCHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TKKLHIKTWGCQMNEYDSSKIADLLGSTHGLELTDNAEDADVLLLNTCSIREKAQEKVF
CCEEEEEEECCCCCCCCHHHHHHHHCCCCCCEECCCCCCCCEEEEECHHHHHHHHHHHH
HQLGRWKMLKEAKPDLIIGVGGCVASQEGEHIRERASFVDIIFGPQTLHRLPEMIDSARS
HHHHHHHHHHHCCCCEEEECCCHHCCCCCHHHHHHHHHEEEEECHHHHHHHHHHHHHHHC
SHGPVVDVSFPEIEKFDRLPEPRADGATAFVSIMEGCNKYCTYCVVPYTRGAEVSRPCDD
CCCCEEECCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHH
VLFEIAQLAAQGVREVNLLGQNVNAYRGATFDGGICSFAELLRLVAAIDGIDRIRFTTSH
HHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHHHHCCCHHEEEECCC
PIEFSDDIVAVYEDTPELVSFLHLPVQSGSDRILNLMKRTHTALEYKAIIRKLRRARPDI
CCCCCCCEEEEECCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
QISSDFIVGFPGETQQDFEQTMQLIAEVDFDMSYSFIFSARPGTPAADMLDDVAEEEKKQ
EECCCEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHH
RLYILQERINQQAMRYSRHMLGTVQRILVEGTSRKNVMELSGRTENNRVVNFAGQPEMIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCEEEEECCCHHHHC
RFVDVEITGVLPNSLRGVVVRSEDEMGLRISESPQSVIARTRKEDDLGVGSYQP
EEEEEEEEEECCCCCCEEEEECCCCCCCEECCCHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA