Click here to switch to the map view.

The map label for this gene is nagB

Identifier: 238920789

GI number: 238920789

Start: 2801859

End: 2802659

Strand: Direct

Name: nagB

Synonym: NT01EI_2908

Alternate gene names: 238920789

Gene position: 2801859-2802659 (Clockwise)

Preceding gene: 238920783

Following gene: 238920790

Centisome position: 73.49

GC content: 53.06

Gene sequence:

>801_bases
ATGAGACTTATTCCTCTGCATAACGCGACCGACGTTGGCCTGTGGTCTGCCCGCCATATCGTTAAACGCATCAACGCGTT
CAAACCTACTGCCGAGCGCCCGTTTGTTCTGGGTCTGCCGACCGGTAGCACCCCGCTGCAAACCTATAAGCGCCTGATCG
AGATCTATCAGGCCGGTGAGGTGAGCTTCCGCCACGTCGTCACCTTTAATATGGATGAATACGTTGGCCTGGCAGAGTCA
CACCCAGAAAGCTATCACAGCTTTATGTATAATAATTTTTTCAACCATATTGATATTCATAAAGAAAATATCAATTTGTT
AAATGGTAACGCACCGGACGTGGACGCCGAGTGCCGCCGCTACGAAGAGAAGATCAGCTCCTACGGCAAGATCAATCTGT
TTATGGGCGGTGTCGGCAACGACGGACATATCGCCTTCAACGAACCGGCCTCTTCGCTGGCTTCACGTACCCGGATTAAG
ACACTGACCGAAGATACCCGTATCGCAAACTCCCGCTTCTTCAACGGTGACATCAGTCTGGTGCCGAAGTATGCGCTGAC
CGTCGGCGTGGGAACCCTGCTGGATGCGGAGGAGGTGATGATCCTGGTGACCGGCCACGCCAAATCCCTGGCACTGCAGG
CTGCGGTCGAAGGCAGCGTCAACCATATGTGGACCATCAGCGCCCTGCAGCTGCATCCCAAGTCCGTGGTAGTCTGCGAT
CAGCCGGCAACCATGGAGCTGAAAGTTAAAACCGTTAACTATTTCCGCGAGCTCGAAGCGGAAAATATGAAAGATCTCTG
A

Upstream 100 bases:

>100_bases
GGCGCGCCAGGCTCCTTAGCAGGAGAGCCGGGAGCCAATGGGTCATCACCGGCGCCGAACCCGTTTTTTGTTCAGTTTCC
CGTTTTGTAAAAGGTGCCCT

Downstream 100 bases:

>100_bases
TAATTACGTCCGGGGGAATGTGATGTACGCCTTAACTCACTGTCGGATTTATACCGGCCACGATATTCTTGACGACCATG
CTGTGGTGATCGCCAATGGT

Product: glucosamine-6-phosphate deaminase

Products: NA

Alternate protein names: GlcN6P deaminase; GNPDA; Glucosamine-6-phosphate isomerase

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MRLIPLHNATDVGLWSARHIVKRINAFKPTAERPFVLGLPTGSTPLQTYKRLIEIYQAGEVSFRHVVTFNMDEYVGLAES
HPESYHSFMYNNFFNHIDIHKENINLLNGNAPDVDAECRRYEEKISSYGKINLFMGGVGNDGHIAFNEPASSLASRTRIK
TLTEDTRIANSRFFNGDISLVPKYALTVGVGTLLDAEEVMILVTGHAKSLALQAAVEGSVNHMWTISALQLHPKSVVVCD
QPATMELKVKTVNYFRELEAENMKDL

Sequences:

>Translated_266_residues
MRLIPLHNATDVGLWSARHIVKRINAFKPTAERPFVLGLPTGSTPLQTYKRLIEIYQAGEVSFRHVVTFNMDEYVGLAES
HPESYHSFMYNNFFNHIDIHKENINLLNGNAPDVDAECRRYEEKISSYGKINLFMGGVGNDGHIAFNEPASSLASRTRIK
TLTEDTRIANSRFFNGDISLVPKYALTVGVGTLLDAEEVMILVTGHAKSLALQAAVEGSVNHMWTISALQLHPKSVVVCD
QPATMELKVKTVNYFRELEAENMKDL
>Mature_266_residues
MRLIPLHNATDVGLWSARHIVKRINAFKPTAERPFVLGLPTGSTPLQTYKRLIEIYQAGEVSFRHVVTFNMDEYVGLAES
HPESYHSFMYNNFFNHIDIHKENINLLNGNAPDVDAECRRYEEKISSYGKINLFMGGVGNDGHIAFNEPASSLASRTRIK
TLTEDTRIANSRFFNGDISLVPKYALTVGVGTLLDAEEVMILVTGHAKSLALQAAVEGSVNHMWTISALQLHPKSVVVCD
QPATMELKVKTVNYFRELEAENMKDL

Specific function: Catalyzes the reversible isomerization-deamination of glucosamine 6-phosphate (GlcN6P) to form fructose 6-phosphate (Fru6P) and ammonium ion

COG id: COG0363

COG function: function code G; 6-phosphogluconolactonase/Glucosamine-6-phosphate isomerase/deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. NagB subfamily

Homologues:

Organism=Homo sapiens, GI13027378, Length=258, Percent_Identity=62.015503875969, Blast_Score=345, Evalue=3e-95,
Organism=Homo sapiens, GI19923881, Length=258, Percent_Identity=60.077519379845, Blast_Score=334, Evalue=4e-92,
Organism=Escherichia coli, GI1786893, Length=266, Percent_Identity=79.3233082706767, Blast_Score=455, Evalue=1e-129,
Organism=Escherichia coli, GI1789530, Length=206, Percent_Identity=28.6407766990291, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI48994958, Length=240, Percent_Identity=26.25, Blast_Score=76, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17554876, Length=258, Percent_Identity=58.9147286821705, Blast_Score=310, Evalue=7e-85,
Organism=Drosophila melanogaster, GI24581960, Length=256, Percent_Identity=60.546875, Blast_Score=345, Evalue=3e-95,
Organism=Drosophila melanogaster, GI19920764, Length=256, Percent_Identity=60.546875, Blast_Score=345, Evalue=3e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NAGB_EDWI9 (C5BGA6)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002934304.1
- ProteinModelPortal:   C5BGA6
- GeneID:   7960193
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_2908
- OMA:   HLSITMG
- ProtClustDB:   PRK00443
- HAMAP:   MF_01241
- InterPro:   IPR006148
- InterPro:   IPR004547
- InterPro:   IPR018321
- PANTHER:   PTHR11280
- TIGRFAMs:   TIGR00502

Pfam domain/function: PF01182 Glucosamine_iso

EC number: =3.5.99.6

Molecular weight: Translated: 29793; Mature: 29793

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS01161 GLC_GALNAC_ISOMERASE

Important sites: ACT_SITE 72-72 ACT_SITE 141-141 ACT_SITE 143-143 ACT_SITE 148-148

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLIPLHNATDVGLWSARHIVKRINAFKPTAERPFVLGLPTGSTPLQTYKRLIEIYQAGE
CEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCC
VSFRHVVTFNMDEYVGLAESHPESYHSFMYNNFFNHIDIHKENINLLNGNAPDVDAECRR
CEEEEEEEECHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEECCCEEECCCCCCCCHHHHH
YEEKISSYGKINLFMGGVGNDGHIAFNEPASSLASRTRIKTLTEDTRIANSRFFNGDISL
HHHHHHCCCEEEEEEECCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCEE
VPKYALTVGVGTLLDAEEVMILVTGHAKSLALQAAVEGSVNHMWTISALQLHPKSVVVCD
CCHHHHHCCHHHHCCCCEEEEEEECCCHHEEEEHHHCCCCCEEEEEEEEEECCCEEEEEC
QPATMELKVKTVNYFRELEAENMKDL
CCCCEEEEEEHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRLIPLHNATDVGLWSARHIVKRINAFKPTAERPFVLGLPTGSTPLQTYKRLIEIYQAGE
CEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCC
VSFRHVVTFNMDEYVGLAESHPESYHSFMYNNFFNHIDIHKENINLLNGNAPDVDAECRR
CEEEEEEEECHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEECCCEEECCCCCCCCHHHHH
YEEKISSYGKINLFMGGVGNDGHIAFNEPASSLASRTRIKTLTEDTRIANSRFFNGDISL
HHHHHHCCCEEEEEEECCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCEE
VPKYALTVGVGTLLDAEEVMILVTGHAKSLALQAAVEGSVNHMWTISALQLHPKSVVVCD
CCHHHHHCCHHHHCCCCEEEEEEECCCHHEEEEHHHCCCCCEEEEEEEEEECCCEEEEEC
QPATMELKVKTVNYFRELEAENMKDL
CCCCEEEEEEHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA