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The map label for this gene is bioB [H]

Identifier: 238920717

GI number: 238920717

Start: 2721425

End: 2722468

Strand: Reverse

Name: bioB [H]

Synonym: NT01EI_2830

Alternate gene names: 238920717

Gene position: 2722468-2721425 (Counterclockwise)

Preceding gene: 238920721

Following gene: 238920716

Centisome position: 71.41

GC content: 63.7

Gene sequence:

>1044_bases
GTGGCAGAGATACCGCATTCCGGGCGCTGGACGCAGGCGCAGGCGCAGGCGTTGTTTGAGCAACCGCTGCTGGAGCTGAT
GTTTCAGGCGCAGCAGGTTCACCGTCAACACTTTGATCCGCGTCAGGTGCAGGTGAGCACCCTGCTATCGATTAAGACCG
GCGCCTGCCCGGAGGACTGTAAATACTGTCCGCAGAGCGCCCGCTATAAAACGGGGCTGGCGGCGGAGCGGCTGATGGAG
GTGGAGCAGGTGCTGGACTCCGCGCGTCGGGCACGCGCCGCCGGATCTACCCGCTTCTGCATGGGGGCGGCGTGGAAAAA
TCCCCACGAACGCGACATGCCCTACCTGGAGCAGATGGTGCGTGGGGTCAAGGCGCTGGGCATGGAGACCTGCATGACTC
TGGGGGCGCTGAACGCCGATCAGGCCCAGCGTCTGGCGCAGGCGGGGCTGGACTACTACAACCACAATCTGGACACCTCA
CCGGAGTATTACGCCAATATCATCACCACCCGTCGCTATCAGGATCGCCTGGATACCCTGGCGCGGGTGCGGGAGGCGGG
GATCAAGGTCTGTTCCGGTGGGATTGTCGGGCTGGGCGAGGCGGTACGCGATCGGGCGGCCCTGCTGGTGGCGCTGGCCA
ATCTGCCGATGCCGCCGGAAAGCGTGCCGATCAATATGCTGGTGAAGGTGGCGGGCACACCGCTGGCGGAAACCCCGGAC
GTTGATCCCTTCGACTTTATCCGCACCATCGCCGCGGCGCGCATCATGATGCCAAGCTCGTACGTGCGCCTCTCCGCCGG
ACGCGAGCAGATGAACGAGCAGACTCAGGCGATGTGCTTTATGGCCGGAGCCAACTCCCTCTTTTACGGCTGTCGCCTGC
TGACCACGCCGAATCCCTCAGAGGATCGCGATCTGCAGCTGTTCCGCAAGCTGGGGCTGAATCCCCGGCAAACGCAGGTA
GCCTATGGCGATAACCAGCAGCAGCAGCTGGCGGAGCAGGTACTGCTCGCGGATGACGACCTGTTTTATGATGCGGCGCG
TTGA

Upstream 100 bases:

>100_bases
TTAATATTGTAAACCATTTAATTGATAACTTGGTTGACATGATATTTCTCCGTTCTACACTGAGCCCACTTTTACCGATT
TGATGAAGGGAGAGGCCACC

Downstream 100 bases:

>100_bases
CAGTGGGCTGGTCTGCACGGTTTAGCCAGGCGTTGGCAGTGCGCAGCGCCGCTGATGCCTATCGTCGCCGAACGGTGCGC
GAGGCGCCCGGCGGACGAGA

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 346

Protein sequence:

>347_residues
MAEIPHSGRWTQAQAQALFEQPLLELMFQAQQVHRQHFDPRQVQVSTLLSIKTGACPEDCKYCPQSARYKTGLAAERLME
VEQVLDSARRARAAGSTRFCMGAAWKNPHERDMPYLEQMVRGVKALGMETCMTLGALNADQAQRLAQAGLDYYNHNLDTS
PEYYANIITTRRYQDRLDTLARVREAGIKVCSGGIVGLGEAVRDRAALLVALANLPMPPESVPINMLVKVAGTPLAETPD
VDPFDFIRTIAAARIMMPSSYVRLSAGREQMNEQTQAMCFMAGANSLFYGCRLLTTPNPSEDRDLQLFRKLGLNPRQTQV
AYGDNQQQQLAEQVLLADDDLFYDAAR

Sequences:

>Translated_347_residues
MAEIPHSGRWTQAQAQALFEQPLLELMFQAQQVHRQHFDPRQVQVSTLLSIKTGACPEDCKYCPQSARYKTGLAAERLME
VEQVLDSARRARAAGSTRFCMGAAWKNPHERDMPYLEQMVRGVKALGMETCMTLGALNADQAQRLAQAGLDYYNHNLDTS
PEYYANIITTRRYQDRLDTLARVREAGIKVCSGGIVGLGEAVRDRAALLVALANLPMPPESVPINMLVKVAGTPLAETPD
VDPFDFIRTIAAARIMMPSSYVRLSAGREQMNEQTQAMCFMAGANSLFYGCRLLTTPNPSEDRDLQLFRKLGLNPRQTQV
AYGDNQQQQLAEQVLLADDDLFYDAAR
>Mature_346_residues
AEIPHSGRWTQAQAQALFEQPLLELMFQAQQVHRQHFDPRQVQVSTLLSIKTGACPEDCKYCPQSARYKTGLAAERLMEV
EQVLDSARRARAAGSTRFCMGAAWKNPHERDMPYLEQMVRGVKALGMETCMTLGALNADQAQRLAQAGLDYYNHNLDTSP
EYYANIITTRRYQDRLDTLARVREAGIKVCSGGIVGLGEAVRDRAALLVALANLPMPPESVPINMLVKVAGTPLAETPDV
DPFDFIRTIAAARIMMPSSYVRLSAGREQMNEQTQAMCFMAGANSLFYGCRLLTTPNPSEDRDLQLFRKLGLNPRQTQVA
YGDNQQQQLAEQVLLADDDLFYDAAR

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=342, Percent_Identity=81.2865497076023, Blast_Score=594, Evalue=1e-171,
Organism=Saccharomyces cerevisiae, GI6321725, Length=318, Percent_Identity=49.0566037735849, Blast_Score=323, Evalue=3e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 38694; Mature: 38563

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEIPHSGRWTQAQAQALFEQPLLELMFQAQQVHRQHFDPRQVQVSTLLSIKTGACPEDC
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHH
KYCPQSARYKTGLAAERLMEVEQVLDSARRARAAGSTRFCMGAAWKNPHERDMPYLEQMV
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCHHHHHHH
RGVKALGMETCMTLGALNADQAQRLAQAGLDYYNHNLDTSPEYYANIITTRRYQDRLDTL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
ARVREAGIKVCSGGIVGLGEAVRDRAALLVALANLPMPPESVPINMLVKVAGTPLAETPD
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCC
VDPFDFIRTIAAARIMMPSSYVRLSAGREQMNEQTQAMCFMAGANSLFYGCRLLTTPNPS
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCEEEECCCCC
EDRDLQLFRKLGLNPRQTQVAYGDNQQQQLAEQVLLADDDLFYDAAR
CCHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHCCCCHHHCCCC
>Mature Secondary Structure 
AEIPHSGRWTQAQAQALFEQPLLELMFQAQQVHRQHFDPRQVQVSTLLSIKTGACPEDC
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHH
KYCPQSARYKTGLAAERLMEVEQVLDSARRARAAGSTRFCMGAAWKNPHERDMPYLEQMV
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCHHHHHHH
RGVKALGMETCMTLGALNADQAQRLAQAGLDYYNHNLDTSPEYYANIITTRRYQDRLDTL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
ARVREAGIKVCSGGIVGLGEAVRDRAALLVALANLPMPPESVPINMLVKVAGTPLAETPD
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCC
VDPFDFIRTIAAARIMMPSSYVRLSAGREQMNEQTQAMCFMAGANSLFYGCRLLTTPNPS
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCEEEECCCCC
EDRDLQLFRKLGLNPRQTQVAYGDNQQQQLAEQVLLADDDLFYDAAR
CCHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]