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The map label for this gene is bioF [H]

Identifier: 238920716

GI number: 238920716

Start: 2720274

End: 2721422

Strand: Reverse

Name: bioF [H]

Synonym: NT01EI_2829

Alternate gene names: 238920716

Gene position: 2721422-2720274 (Counterclockwise)

Preceding gene: 238920717

Following gene: 238920715

Centisome position: 71.39

GC content: 66.49

Gene sequence:

>1149_bases
GTGGGCTGGTCTGCACGGTTTAGCCAGGCGTTGGCAGTGCGCAGCGCCGCTGATGCCTATCGTCGCCGAACGGTGCGCGA
GGCGCCCGGCGGACGAGAGATCATCGTTGAGGGACGGCACTATATTAATTTTTCATCCAATGATTATCTGGGGCTGAGTC
AGCATCCGGCGCCGATCGCGGCCTGGCAGCAGGGGCTGGTGCGCTACGGCAGCGGCTCCGGCGGTTCGGCACACGTGACC
GGGCAAACCACGGCCCACGCGGCGCTGGAGCGTGCGCTGGCCGAGTGGCTCGGCTACCCGCGTGCGCTGCTGTTTATCTC
CGGTTTTGCCGCCAATCAGGCACTGATCCTGGCGCTGATGCAGCGTCAGGATAGGGTGCTGGCGGATAGGCTGAGCCACG
CCTCGCTGATGGAGGCGGCGATGCTGGCACCGTCATCCCTGCGTCGTTTTCAGCATAATCAGCCCGCCTCGCTTGCGGCC
TTGCTGGCGGCTCCCTGTGAGGGTGAGACACTGGTGGTGACCGAGGGGGTCTTCAGCATGGACGGCGACAGCGCCCCGTT
GGCGGCGCTGCGGGCGCAGTGCGATCGCCACGGCGCCTGGTTGATGGTCGACGACGCTCACGGTATCGGTATATGCGGGC
GTAAGGGGCGCGGTAGCTGCGATGCGCAGGGGATTCGCCCCGATCTGCTGATCGTCACCTTTGGCAAGGCCTTTGGCATC
GGCGGTGCGGCGCTGCTGTGCGGCGAGGCGACGGCGGAATACCTGCTGCAGTTTGCCCGCCATCTGATCTACAGCACCAG
CATGCCGCCGGCGCAGGCCTGCGCCCTACAGGCTGCGCTGCAGGTGGTGCGCGGGGCCGATGCGCCACGGGCGCGGTTGG
CGCAACATGTGGCGCAGTTACGCCGGGGGCTGACGGGCGGTGAGTTCACTCTGGCGGCGTCCGATAGCGCTATCCAGCCG
CTGATCGTCGGGGATAATCAGGCGGCGCTGACGTTGGCACAACGCCTGCGTCAGCGCGGCCTGTGGGTGTGCGCCATGCG
TCCGCCCACCGTACCACCCGGCGGCGCGCGCCTGCGAATTACTCTGAGTGCAGCGCATTATCCGCAGGATATTGAGCGGT
TGCTGGAGGCATTGCATGAGTCAGTGTGA

Upstream 100 bases:

>100_bases
GGCAAACGCAGGTAGCCTATGGCGATAACCAGCAGCAGCAGCTGGCGGAGCAGGTACTGCTCGCGGATGACGACCTGTTT
TATGATGCGGCGCGTTGACA

Downstream 100 bases:

>100_bases
CTGGGTAAACAAGCCAGCGCTGGCGGCCGCCTTTAGCCGCGCCGCGCACAGCTATGACGGTGCGGCGGCCTTCCAGCGCC
GCTGTGGGGAACGCCTGCTG

Product: 8-amino-7-oxononanoate synthase

Products: NA

Alternate protein names: AONS; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; L-alanine--pimeloyl-CoA ligase [H]

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MGWSARFSQALAVRSAADAYRRRTVREAPGGREIIVEGRHYINFSSNDYLGLSQHPAPIAAWQQGLVRYGSGSGGSAHVT
GQTTAHAALERALAEWLGYPRALLFISGFAANQALILALMQRQDRVLADRLSHASLMEAAMLAPSSLRRFQHNQPASLAA
LLAAPCEGETLVVTEGVFSMDGDSAPLAALRAQCDRHGAWLMVDDAHGIGICGRKGRGSCDAQGIRPDLLIVTFGKAFGI
GGAALLCGEATAEYLLQFARHLIYSTSMPPAQACALQAALQVVRGADAPRARLAQHVAQLRRGLTGGEFTLAASDSAIQP
LIVGDNQAALTLAQRLRQRGLWVCAMRPPTVPPGGARLRITLSAAHYPQDIERLLEALHESV

Sequences:

>Translated_382_residues
MGWSARFSQALAVRSAADAYRRRTVREAPGGREIIVEGRHYINFSSNDYLGLSQHPAPIAAWQQGLVRYGSGSGGSAHVT
GQTTAHAALERALAEWLGYPRALLFISGFAANQALILALMQRQDRVLADRLSHASLMEAAMLAPSSLRRFQHNQPASLAA
LLAAPCEGETLVVTEGVFSMDGDSAPLAALRAQCDRHGAWLMVDDAHGIGICGRKGRGSCDAQGIRPDLLIVTFGKAFGI
GGAALLCGEATAEYLLQFARHLIYSTSMPPAQACALQAALQVVRGADAPRARLAQHVAQLRRGLTGGEFTLAASDSAIQP
LIVGDNQAALTLAQRLRQRGLWVCAMRPPTVPPGGARLRITLSAAHYPQDIERLLEALHESV
>Mature_381_residues
GWSARFSQALAVRSAADAYRRRTVREAPGGREIIVEGRHYINFSSNDYLGLSQHPAPIAAWQQGLVRYGSGSGGSAHVTG
QTTAHAALERALAEWLGYPRALLFISGFAANQALILALMQRQDRVLADRLSHASLMEAAMLAPSSLRRFQHNQPASLAAL
LAAPCEGETLVVTEGVFSMDGDSAPLAALRAQCDRHGAWLMVDDAHGIGICGRKGRGSCDAQGIRPDLLIVTFGKAFGIG
GAALLCGEATAEYLLQFARHLIYSTSMPPAQACALQAALQVVRGADAPRARLAQHVAQLRRGLTGGEFTLAASDSAIQPL
IVGDNQAALTLAQRLRQRGLWVCAMRPPTVPPGGARLRITLSAAHYPQDIERLLEALHESV

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A, and carbon dioxide [H]

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657118, Length=361, Percent_Identity=34.3490304709141, Blast_Score=192, Evalue=5e-49,
Organism=Homo sapiens, GI284448556, Length=343, Percent_Identity=34.402332361516, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI83977442, Length=341, Percent_Identity=31.9648093841642, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI83977440, Length=343, Percent_Identity=31.7784256559767, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI83977444, Length=343, Percent_Identity=31.7784256559767, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI119220554, Length=360, Percent_Identity=30, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI4758668, Length=360, Percent_Identity=28.8888888888889, Blast_Score=142, Evalue=5e-34,
Organism=Homo sapiens, GI4502025, Length=342, Percent_Identity=32.1637426900585, Blast_Score=141, Evalue=8e-34,
Organism=Homo sapiens, GI40316939, Length=342, Percent_Identity=32.1637426900585, Blast_Score=141, Evalue=8e-34,
Organism=Homo sapiens, GI5454084, Length=297, Percent_Identity=27.2727272727273, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI1786993, Length=351, Percent_Identity=64.6723646723647, Blast_Score=436, Evalue=1e-124,
Organism=Escherichia coli, GI1790046, Length=365, Percent_Identity=35.8904109589041, Blast_Score=221, Evalue=5e-59,
Organism=Caenorhabditis elegans, GI71994529, Length=382, Percent_Identity=31.151832460733, Blast_Score=205, Evalue=3e-53,
Organism=Caenorhabditis elegans, GI17560914, Length=374, Percent_Identity=27.0053475935829, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17560912, Length=374, Percent_Identity=27.0053475935829, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI32566772, Length=360, Percent_Identity=27.2222222222222, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI71982625, Length=303, Percent_Identity=22.4422442244224, Blast_Score=81, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI71982617, Length=303, Percent_Identity=22.4422442244224, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320438, Length=363, Percent_Identity=28.099173553719, Blast_Score=137, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6320267, Length=360, Percent_Identity=28.3333333333333, Blast_Score=135, Evalue=9e-33,
Organism=Saccharomyces cerevisiae, GI6323954, Length=187, Percent_Identity=31.5508021390374, Blast_Score=93, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24662918, Length=353, Percent_Identity=30.5949008498584, Blast_Score=190, Evalue=1e-48,
Organism=Drosophila melanogaster, GI17137420, Length=341, Percent_Identity=30.2052785923754, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI17136286, Length=365, Percent_Identity=26.5753424657534, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24653280, Length=267, Percent_Identity=23.9700374531835, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24653276, Length=267, Percent_Identity=23.9700374531835, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24653278, Length=267, Percent_Identity=23.9700374531835, Blast_Score=79, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022834
- InterPro:   IPR001917
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =2.3.1.47 [H]

Molecular weight: Translated: 40732; Mature: 40601

Theoretical pI: Translated: 8.87; Mature: 8.87

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGWSARFSQALAVRSAADAYRRRTVREAPGGREIIVEGRHYINFSSNDYLGLSQHPAPIA
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCEEEEECCCCCCCCCCCCCHHH
AWQQGLVRYGSGSGGSAHVTGQTTAHAALERALAEWLGYPRALLFISGFAANQALILALM
HHHHHHHEECCCCCCCEEECCCHHHHHHHHHHHHHHHCCCEEEHEEECCCCCHHHHHHHH
QRQDRVLADRLSHASLMEAAMLAPSSLRRFQHNQPASLAALLAAPCEGETLVVTEGVFSM
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHCCCCCCEEEEECCEEEC
DGDSAPLAALRAQCDRHGAWLMVDDAHGIGICGRKGRGSCDAQGIRPDLLIVTFGKAFGI
CCCCCHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCCCCCCCCCCCCEEEEEECHHHCC
GGAALLCGEATAEYLLQFARHLIYSTSMPPAQACALQAALQVVRGADAPRARLAQHVAQL
CCHHEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
RRGLTGGEFTLAASDSAIQPLIVGDNQAALTLAQRLRQRGLWVCAMRPPTVPPGGARLRI
HCCCCCCCEEEEECCCCCCCEEECCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEE
TLSAAHYPQDIERLLEALHESV
EEECCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GWSARFSQALAVRSAADAYRRRTVREAPGGREIIVEGRHYINFSSNDYLGLSQHPAPIA
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCEEEEECCCCCCCCCCCCCHHH
AWQQGLVRYGSGSGGSAHVTGQTTAHAALERALAEWLGYPRALLFISGFAANQALILALM
HHHHHHHEECCCCCCCEEECCCHHHHHHHHHHHHHHHCCCEEEHEEECCCCCHHHHHHHH
QRQDRVLADRLSHASLMEAAMLAPSSLRRFQHNQPASLAALLAAPCEGETLVVTEGVFSM
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHCCCCCCEEEEECCEEEC
DGDSAPLAALRAQCDRHGAWLMVDDAHGIGICGRKGRGSCDAQGIRPDLLIVTFGKAFGI
CCCCCHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCCCCCCCCCCCCEEEEEECHHHCC
GGAALLCGEATAEYLLQFARHLIYSTSMPPAQACALQAALQVVRGADAPRARLAQHVAQL
CCHHEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
RRGLTGGEFTLAASDSAIQPLIVGDNQAALTLAQRLRQRGLWVCAMRPPTVPPGGARLRI
HCCCCCCCEEEEECCCCCCCEEECCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEE
TLSAAHYPQDIERLLEALHESV
EEECCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]