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The map label for this gene is mnmC [H]

Identifier: 238920616

GI number: 238920616

Start: 2619871

End: 2621895

Strand: Direct

Name: mnmC [H]

Synonym: NT01EI_2728

Alternate gene names: 238920616

Gene position: 2619871-2621895 (Clockwise)

Preceding gene: 238920613

Following gene: 238920620

Centisome position: 68.72

GC content: 66.57

Gene sequence:

>2025_bases
GTGAACCATACATCGATACAAAGTGCATCGCTGAGCTGGAATCAACAGGGTACGCCTGTTTCGCAGCAGTTTGACGACGT
CTACTTTTCCAATCAGGACGGGCTGGCGGAAACCCGTCACGTCTTCCTGCAGGGCAACGGGCTGCCGCAGCGCTGGCTGA
CTGGCTCCCGGCCCGATTTCACTGTCGCCGAAACCGGCTTCGGTACCGGGCTGAATTTTCTCACCCTGTGGCAGGCTCAC
CGTGCTTTCCGACTGGCTTATCCCGATAGGGGACCGTCGCGCCTGCACTTCATTAGCTTTGAAAAATACCCACTGCGCCG
CGACGACCTGCGCGCTGCCCACGCTGCCTGGCCGGAGCTGGCAGAACTCAGCGAACAGCTGTACGCTGTGTGGCCCGACG
CCCTGCCTGGCTGCCACCGTCTGCACCCGGCGCCACACATCACGCTGGATCTATGGTTCGGCGACGTCAACGCGCTGCTG
CCACAGCTGCCCGACGGTCTCCATGGCCACGTGGACGCCTGGTTTCTGGATGGCTTTGCGCCGGCGAAGAACCCGGATAT
GTGGACCCCTGCGCTCTTCAACACCATGCTGCGCCTGGCCCGTCCCGGCGCGACCTTCGCCACCTTTACCGCCGCCGGAT
TTGTACGCCGCGGCCTGAGCCAGGCCGGATTTGAGGTCAGTAAAGTCAAAGGATTCGGACAAAAGCGGGAGTGTCTGGCG
GGACAGCGCCCTGCAGCACTGCCGCCCGATGTGCTGCAGGCACCCTGGTATGCCCGCCCGGCGGCCCGCCGCCCTGAAGA
AGTTGCACTGATCGGCGGCGGTATCGCCTCCGCCCTGTGCGCCCTGGCACTGCAGCGGCGCGGCAGCCGCGTTACCCTCT
ACTGCGCCGATCCGCAGGCGGCGCAGGGCGCCTCCGGCAACCGTCAGGGGGCACTCTACCCCCTTCTTAACGGCCGCGAT
GATGCGCTGGAGCGCTTTTACGCCCGCGCCTTTCCCTATGCCCTGCGCACCTACGATGCGCTGGCGGCCCAAGGCGCCGA
CTTTCCCCACCGATGGTGCGGCGTCAGTCAGCTGGGTTACGATGACGCCAGCCAGAGAAAAATAGCGCAGCTGCTGAACG
CCGGTTGGCCAGAGTGGCTGGCGCAGGGGGGGGATGCCGCCGATCTCAGCCTGCGCTGCGGCCTGCCGCTGCCGGTTGGC
GGCGTCTGCTATCCCCTCGGTGGCTGGCTGGATCCGGCTGAGCTGACCCGCCAGACCCTGGCGCTGGCGGAACGTCAGGG
GGCCACCCTGTACTTTAACCATGAGGTCCAGCGGCTGCTACCGGAACCAGAGGGCTGGCGTCTACAGCTGGCCAACGGCG
CTACCCGTCTGCACGGCTGCGTCGTGCTGGCCGGCGGCGCCCGCACCGGTGAGTTCAACCAAAGCGATGCGCTGCCGCTC
TACCCGGTGCGCGGCCAGGTGAGCCATATTCCCACCGCCCCGGCGCTGGCGTCGCTGCGCCAGGTGCTGTGCCATGACGG
CTATCTGACCCCCTGCAGCCCGGACTACGGTACCCACTGCCTCGGTGCCAGCTACCGACGCAACTGCACCGATTTGGAGT
ACAGCCCGCAGGAGCAACAGGAAAACCGCCAGCGGCTAATCGCGTCTCTGCCGCAGGTGACATGGCCGCAGCAGGTGGAC
GTCAGCGCCGCGGCAGCGCGGATCGGCATACGCTGCGCCGTGCGCGATCATCTGCCGCTGGTCGGCGCGCTGCCGGACGC
CCCAGCGCTGCAGACGCGCTATCCGCATCCTCGCCCGCAGCAGCGCGTCCCCGCGGAGACCAACGCCGCCGCGCCGCACC
ATCCGGATCTGTTTATCCTGGCCGCCCTCGGCTCACGCGGTCTGTGCAGCGCGCCGCTATGCGCCGAGCTGCTGGCCGGA
CAAATTCACGACGAAGGGCTGCCGCTGGAGCAAGATCTGCTGGACGCACTCAGCCCCAACCGTTTCTGGCTGCGTAAATG
GCTTAAAGGGAAAGGTCCGGCATGA

Upstream 100 bases:

>100_bases
GCAAGTCCGATCAGCGCTGGTAAAACCAGATAGCGCTAAATTTGCCGCCCCGGGCACAGACCGTTAAAATCGTCCACTGA
CTGATTTAGCGGTAAAAAAC

Downstream 100 bases:

>100_bases
TGCATACGGCGGTGGGGGACACGCCGCCGCCCTTCTCGCGTTATCAGAATACGGCGTCAACGCGGGGGGCCAGCCCCGGA
TATAGATCCACCGCACAGCC

Product: protein YfcK

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase [H]

Number of amino acids: Translated: 674; Mature: 674

Protein sequence:

>674_residues
MNHTSIQSASLSWNQQGTPVSQQFDDVYFSNQDGLAETRHVFLQGNGLPQRWLTGSRPDFTVAETGFGTGLNFLTLWQAH
RAFRLAYPDRGPSRLHFISFEKYPLRRDDLRAAHAAWPELAELSEQLYAVWPDALPGCHRLHPAPHITLDLWFGDVNALL
PQLPDGLHGHVDAWFLDGFAPAKNPDMWTPALFNTMLRLARPGATFATFTAAGFVRRGLSQAGFEVSKVKGFGQKRECLA
GQRPAALPPDVLQAPWYARPAARRPEEVALIGGGIASALCALALQRRGSRVTLYCADPQAAQGASGNRQGALYPLLNGRD
DALERFYARAFPYALRTYDALAAQGADFPHRWCGVSQLGYDDASQRKIAQLLNAGWPEWLAQGGDAADLSLRCGLPLPVG
GVCYPLGGWLDPAELTRQTLALAERQGATLYFNHEVQRLLPEPEGWRLQLANGATRLHGCVVLAGGARTGEFNQSDALPL
YPVRGQVSHIPTAPALASLRQVLCHDGYLTPCSPDYGTHCLGASYRRNCTDLEYSPQEQQENRQRLIASLPQVTWPQQVD
VSAAAARIGIRCAVRDHLPLVGALPDAPALQTRYPHPRPQQRVPAETNAAAPHHPDLFILAALGSRGLCSAPLCAELLAG
QIHDEGLPLEQDLLDALSPNRFWLRKWLKGKGPA

Sequences:

>Translated_674_residues
MNHTSIQSASLSWNQQGTPVSQQFDDVYFSNQDGLAETRHVFLQGNGLPQRWLTGSRPDFTVAETGFGTGLNFLTLWQAH
RAFRLAYPDRGPSRLHFISFEKYPLRRDDLRAAHAAWPELAELSEQLYAVWPDALPGCHRLHPAPHITLDLWFGDVNALL
PQLPDGLHGHVDAWFLDGFAPAKNPDMWTPALFNTMLRLARPGATFATFTAAGFVRRGLSQAGFEVSKVKGFGQKRECLA
GQRPAALPPDVLQAPWYARPAARRPEEVALIGGGIASALCALALQRRGSRVTLYCADPQAAQGASGNRQGALYPLLNGRD
DALERFYARAFPYALRTYDALAAQGADFPHRWCGVSQLGYDDASQRKIAQLLNAGWPEWLAQGGDAADLSLRCGLPLPVG
GVCYPLGGWLDPAELTRQTLALAERQGATLYFNHEVQRLLPEPEGWRLQLANGATRLHGCVVLAGGARTGEFNQSDALPL
YPVRGQVSHIPTAPALASLRQVLCHDGYLTPCSPDYGTHCLGASYRRNCTDLEYSPQEQQENRQRLIASLPQVTWPQQVD
VSAAAARIGIRCAVRDHLPLVGALPDAPALQTRYPHPRPQQRVPAETNAAAPHHPDLFILAALGSRGLCSAPLCAELLAG
QIHDEGLPLEQDLLDALSPNRFWLRKWLKGKGPA
>Mature_674_residues
MNHTSIQSASLSWNQQGTPVSQQFDDVYFSNQDGLAETRHVFLQGNGLPQRWLTGSRPDFTVAETGFGTGLNFLTLWQAH
RAFRLAYPDRGPSRLHFISFEKYPLRRDDLRAAHAAWPELAELSEQLYAVWPDALPGCHRLHPAPHITLDLWFGDVNALL
PQLPDGLHGHVDAWFLDGFAPAKNPDMWTPALFNTMLRLARPGATFATFTAAGFVRRGLSQAGFEVSKVKGFGQKRECLA
GQRPAALPPDVLQAPWYARPAARRPEEVALIGGGIASALCALALQRRGSRVTLYCADPQAAQGASGNRQGALYPLLNGRD
DALERFYARAFPYALRTYDALAAQGADFPHRWCGVSQLGYDDASQRKIAQLLNAGWPEWLAQGGDAADLSLRCGLPLPVG
GVCYPLGGWLDPAELTRQTLALAERQGATLYFNHEVQRLLPEPEGWRLQLANGATRLHGCVVLAGGARTGEFNQSDALPL
YPVRGQVSHIPTAPALASLRQVLCHDGYLTPCSPDYGTHCLGASYRRNCTDLEYSPQEQQENRQRLIASLPQVTWPQQVD
VSAAAARIGIRCAVRDHLPLVGALPDAPALQTRYPHPRPQQRVPAETNAAAPHHPDLFILAALGSRGLCSAPLCAELLAG
QIHDEGLPLEQDLLDALSPNRFWLRKWLKGKGPA

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family [H]

Homologues:

Organism=Escherichia coli, GI87082084, Length=673, Percent_Identity=56.4635958395245, Blast_Score=714, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610 [H]

Pfam domain/function: PF01266 DAO; PF05430 DUF752 [H]

EC number: =2.1.1.61 [H]

Molecular weight: Translated: 73799; Mature: 73799

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: PS00732 RIBOSOMAL_S16

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHTSIQSASLSWNQQGTPVSQQFDDVYFSNQDGLAETRHVFLQGNGLPQRWLTGSRPDF
CCCCCCCCCCCCCCCCCCCHHHHHHHHEECCCCCCCCEEEEEEECCCCCHHHCCCCCCCC
TVAETGFGTGLNFLTLWQAHRAFRLAYPDRGPSRLHFISFEKYPLRRDDLRAAHAAWPEL
EEEECCCCCCCHHHHHHHHHHEEEEECCCCCCCEEEEEEECCCCCCHHHHHHHHHCCHHH
AELSEQLYAVWPDALPGCHRLHPAPHITLDLWFGDVNALLPQLPDGLHGHVDAWFLDGFA
HHHHHHHHHHCCCCCCCCHHCCCCCEEEEEEEECCHHHHHHHCCCCCCCCCEEEEECCCC
PAKNPDMWTPALFNTMLRLARPGATFATFTAAGFVRRGLSQAGFEVSKVKGFGQKRECLA
CCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHC
GQRPAALPPDVLQAPWYARPAARRPEEVALIGGGIASALCALALQRRGSRVTLYCADPQA
CCCCCCCCHHHHCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCEEEEEECCCCC
AQGASGNRQGALYPLLNGRDDALERFYARAFPYALRTYDALAAQGADFPHRWCGVSQLGY
CCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCC
DDASQRKIAQLLNAGWPEWLAQGGDAADLSLRCGLPLPVGGVCYPLGGWLDPAELTRQTL
CCHHHHHHHHHHHCCCHHHHHCCCCCCCEEEECCCCCCCCCEEECCCCCCCHHHHHHHHH
ALAERQGATLYFNHEVQRLLPEPEGWRLQLANGATRLHGCVVLAGGARTGEFNQSDALPL
HHHHHCCCEEEECCHHHHHCCCCCCCEEEECCCCCEEEEEEEEECCCCCCCCCCCCCCEE
YPVRGQVSHIPTAPALASLRQVLCHDGYLTPCSPDYGTHCLGASYRRNCTDLEYSPQEQQ
EECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCHHHH
ENRQRLIASLPQVTWPQQVDVSAAAARIGIRCAVRDHLPLVGALPDAPALQTRYPHPRPQ
HHHHHHHHHCCCCCCCCCCCHHHHHHHHCEEEEECCCCCEEECCCCCCCCCCCCCCCCCC
QRVPAETNAAAPHHPDLFILAALGSRGLCSAPLCAELLAGQIHDEGLPLEQDLLDALSPN
CCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCC
RFWLRKWLKGKGPA
HHHHHHHHCCCCCC
>Mature Secondary Structure
MNHTSIQSASLSWNQQGTPVSQQFDDVYFSNQDGLAETRHVFLQGNGLPQRWLTGSRPDF
CCCCCCCCCCCCCCCCCCCHHHHHHHHEECCCCCCCCEEEEEEECCCCCHHHCCCCCCCC
TVAETGFGTGLNFLTLWQAHRAFRLAYPDRGPSRLHFISFEKYPLRRDDLRAAHAAWPEL
EEEECCCCCCCHHHHHHHHHHEEEEECCCCCCCEEEEEEECCCCCCHHHHHHHHHCCHHH
AELSEQLYAVWPDALPGCHRLHPAPHITLDLWFGDVNALLPQLPDGLHGHVDAWFLDGFA
HHHHHHHHHHCCCCCCCCHHCCCCCEEEEEEEECCHHHHHHHCCCCCCCCCEEEEECCCC
PAKNPDMWTPALFNTMLRLARPGATFATFTAAGFVRRGLSQAGFEVSKVKGFGQKRECLA
CCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHC
GQRPAALPPDVLQAPWYARPAARRPEEVALIGGGIASALCALALQRRGSRVTLYCADPQA
CCCCCCCCHHHHCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCEEEEEECCCCC
AQGASGNRQGALYPLLNGRDDALERFYARAFPYALRTYDALAAQGADFPHRWCGVSQLGY
CCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCC
DDASQRKIAQLLNAGWPEWLAQGGDAADLSLRCGLPLPVGGVCYPLGGWLDPAELTRQTL
CCHHHHHHHHHHHCCCHHHHHCCCCCCCEEEECCCCCCCCCEEECCCCCCCHHHHHHHHH
ALAERQGATLYFNHEVQRLLPEPEGWRLQLANGATRLHGCVVLAGGARTGEFNQSDALPL
HHHHHCCCEEEECCHHHHHCCCCCCCEEEECCCCCEEEEEEEEECCCCCCCCCCCCCCEE
YPVRGQVSHIPTAPALASLRQVLCHDGYLTPCSPDYGTHCLGASYRRNCTDLEYSPQEQQ
EECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCHHHH
ENRQRLIASLPQVTWPQQVDVSAAAARIGIRCAVRDHLPLVGALPDAPALQTRYPHPRPQ
HHHHHHHHHCCCCCCCCCCCHHHHHHHHCEEEEECCCCCEEECCCCCCCCCCCCCCCCCC
QRVPAETNAAAPHHPDLFILAALGSRGLCSAPLCAELLAGQIHDEGLPLEQDLLDALSPN
CCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCC
RFWLRKWLKGKGPA
HHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA