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The map label for this gene is truA [H]

Identifier: 238920608

GI number: 238920608

Start: 2613249

End: 2614076

Strand: Reverse

Name: truA [H]

Synonym: NT01EI_2720

Alternate gene names: 238920608

Gene position: 2614076-2613249 (Counterclockwise)

Preceding gene: 238920609

Following gene: 238920607

Centisome position: 68.57

GC content: 66.06

Gene sequence:

>828_bases
ATGTCGCAGCAACCGTTGACCGGGCAGACACCGCGCCCGGAATATACTCTGGCGCTGGGGATCGAGTATGACGGCAGCCG
CTACGCCGGCTGGCAGCGCCAGCGGGAGGTCGACAGCGTCCAGGCGCGCCTGGAGGCGGCGCTGTCGCGCGTCGCCAGCG
AGCCGGTGACGGTGTTCTGCGCTGGGCGTACCGACGCCGGGGTACACGCCACCGGTCAGGTGGTGCACTTCACCACCCAT
GCCCTGCGTCAGGAGGGCGCCTGGACCATGGGCGTCAACGCCCATCTGCCCAAGGATGTCGCGGTACGCTGGGTTAAGGA
TGTCAGTCCCGATTTTCACGCGCGCTTCAGCGCGACGGCGCGCCGCTATCGCTACGTCATCTATAATCATCGCTACCGCC
CGGCGGTGCTGCAGAGCGGAGTCACCCATTTCTATCTGCCGCTGGACGCAGAACGCATGGCGCGCGCCGGACAGGCGCTG
CTGGGGGAGAACGACTTCAGCGCCTTTCGCGCCGCCCAGTGTCAGTCCCGTACCCCGTGGCGCAACGTCTCCCACCTGAG
GGTCGACCGCTACGGCTCCTACGTGGTGGTCGATATCAAGGCCAATGCCTTTGTGCATCATATGGTGCGTAATATCGTCG
GCAGTCTGCTTGAGGTGGGGTGCGGCAACCAGCCGGAAAGCTGGATCGCCGGGCTGCTGGCGGGCCGGGATCGTACCCTG
GCTGCCGCGACTGCCAAGGCGGCCGGGCTGTATCTGGTCGCCGTAGACTACCCGGCGCAATTTGCCCTGCCGCAGTCGCC
GATGGGGCCGCTGTTTTTACCCGATTAA

Upstream 100 bases:

>100_bases
AGGCCCTGCAGTTCTGGTCGGTGGCGGACAATGTTCGCTTCGGCGGGGCGCTGATGGCGCTGGAGGTAGCAGAACGCCTG
ATGCAGGAGTATCTCTACTG

Downstream 100 bases:

>100_bases
TCTCCGGAGCCGTTATGGAATACCTACAGTGGATTGTTGATTTTATTCTACATATTGATGTGCACCTGGCACAGCTGGTC
GCGCAGTATGGCGTGTGGAT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MSQQPLTGQTPRPEYTLALGIEYDGSRYAGWQRQREVDSVQARLEAALSRVASEPVTVFCAGRTDAGVHATGQVVHFTTH
ALRQEGAWTMGVNAHLPKDVAVRWVKDVSPDFHARFSATARRYRYVIYNHRYRPAVLQSGVTHFYLPLDAERMARAGQAL
LGENDFSAFRAAQCQSRTPWRNVSHLRVDRYGSYVVVDIKANAFVHHMVRNIVGSLLEVGCGNQPESWIAGLLAGRDRTL
AAATAKAAGLYLVAVDYPAQFALPQSPMGPLFLPD

Sequences:

>Translated_275_residues
MSQQPLTGQTPRPEYTLALGIEYDGSRYAGWQRQREVDSVQARLEAALSRVASEPVTVFCAGRTDAGVHATGQVVHFTTH
ALRQEGAWTMGVNAHLPKDVAVRWVKDVSPDFHARFSATARRYRYVIYNHRYRPAVLQSGVTHFYLPLDAERMARAGQAL
LGENDFSAFRAAQCQSRTPWRNVSHLRVDRYGSYVVVDIKANAFVHHMVRNIVGSLLEVGCGNQPESWIAGLLAGRDRTL
AAATAKAAGLYLVAVDYPAQFALPQSPMGPLFLPD
>Mature_274_residues
SQQPLTGQTPRPEYTLALGIEYDGSRYAGWQRQREVDSVQARLEAALSRVASEPVTVFCAGRTDAGVHATGQVVHFTTHA
LRQEGAWTMGVNAHLPKDVAVRWVKDVSPDFHARFSATARRYRYVIYNHRYRPAVLQSGVTHFYLPLDAERMARAGQALL
GENDFSAFRAAQCQSRTPWRNVSHLRVDRYGSYVVVDIKANAFVHHMVRNIVGSLLEVGCGNQPESWIAGLLAGRDRTLA
AATAKAAGLYLVAVDYPAQFALPQSPMGPLFLPD

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=306, Percent_Identity=30.0653594771242, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1788657, Length=270, Percent_Identity=76.6666666666667, Blast_Score=435, Evalue=1e-123,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 30436; Mature: 30305

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQQPLTGQTPRPEYTLALGIEYDGSRYAGWQRQREVDSVQARLEAALSRVASEPVTVFC
CCCCCCCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
AGRTDAGVHATGQVVHFTTHALRQEGAWTMGVNAHLPKDVAVRWVKDVSPDFHARFSATA
ECCCCCCCCCCCCEEEEHHHHHHHCCCEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHH
RRYRYVIYNHRYRPAVLQSGVTHFYLPLDAERMARAGQALLGENDFSAFRAAQCQSRTPW
HHEEEEEEECCCCHHHHHCCCEEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCC
RNVSHLRVDRYGSYVVVDIKANAFVHHMVRNIVGSLLEVGCGNQPESWIAGLLAGRDRTL
CCCCEEEEECCCCEEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCH
AAATAKAAGLYLVAVDYPAQFALPQSPMGPLFLPD
HHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SQQPLTGQTPRPEYTLALGIEYDGSRYAGWQRQREVDSVQARLEAALSRVASEPVTVFC
CCCCCCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
AGRTDAGVHATGQVVHFTTHALRQEGAWTMGVNAHLPKDVAVRWVKDVSPDFHARFSATA
ECCCCCCCCCCCCEEEEHHHHHHHCCCEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHH
RRYRYVIYNHRYRPAVLQSGVTHFYLPLDAERMARAGQALLGENDFSAFRAAQCQSRTPW
HHEEEEEEECCCCHHHHHCCCEEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCC
RNVSHLRVDRYGSYVVVDIKANAFVHHMVRNIVGSLLEVGCGNQPESWIAGLLAGRDRTL
CCCCEEEEECCCCEEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCH
AAATAKAAGLYLVAVDYPAQFALPQSPMGPLFLPD
HHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA