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The map label for this gene is nuoF [H]

Identifier: 238920563

GI number: 238920563

Start: 2570915

End: 2572261

Strand: Reverse

Name: nuoF [H]

Synonym: NT01EI_2675

Alternate gene names: 238920563

Gene position: 2572261-2570915 (Counterclockwise)

Preceding gene: 238920564

Following gene: 238920562

Centisome position: 67.47

GC content: 63.47

Gene sequence:

>1347_bases
ATGAGAGAGATTATTCGTACCGTAGAATCTCACCCGCTGACCTGGCGCCTGCGCGATGACCAACAGCCAGTATGGCTGGA
GGAGTATCGCGCCAAGAACGGCTACCACGCCGCAGAGCGTGCCCTGAAGGGGATGGCGCAGGATGAGGTGGTGACGCTGG
TCAAGGACGCCGGGCTTAAAGGGCGAGGCGGGGCTGGCTTTTCCACCGGGCTGAAGTGGAGTCTGATGCCCAAGGACGAA
AGCATGAACGTCCGCTACCTGCTGTGTAACGCGGATGAGATGGAGCCGGGCACCTATAAAGACCGCCTGCTGATGGAGCA
GCTGCCGCACCTGCTGGTGGAGGGGATGCTGATCGGCGCCTATGCGCTGAAGGCTTACCGCGGTTATATCTTCCTGCGCG
GCGAGTATGTGAGGGCGGCGGAGAATCTGCGCCGGGCGATCGACGAGGCCCGCGCGGCGGGGCTGCTGGGTAAGAATATT
TTTGGCAGTGGTTTCGACTTCGATCTGTTCGTGCATACTGGCGCCGGGCGTTACATCTGCGGCGAAGAGACGGCGCTGAT
CAACTCGCTGGAGGGGCGCCGCGCCAACCCGCGCGCCAAGCCGCCGTTCCCGGCCAGCGCCGGTGTCTGGGGCAAGCCGA
CCTGTGTGAATAACGTGGAGACGCTGTGCAACGTGCCGGCGATCATCGAGCACGGTGTCGCCTGGTATCAGGGATTGTCT
GCCGGAAAGAGTCAGGATGCCGGCACCAAGTTGATGGGCTTCTCCGGTCGGGTGAAAAATCCCGGTCTGTGGGAGCTGCC
CTTTGGCACGACAGCGCGCGAAATCCTGGAGGACTACGCCGGGGGGATGCGCGATGGGCTGAAGCTGAAGGCGTGGCAAC
CGGGCGGGGCGGGTACCGATTTTCTGACCGAGGCGCACCTGGATCTGCCGATGGACTTCGCCAGCATCGGCAAGGCGGGC
AGCCGTCTGGGCACTGCGCTGGCGATGGCGGTCGACCATGAGATCAACATGGTCTCGCTGCTGTGCAATTTGGAGAGTTT
CTTCGCCCGTGAGTCCTGCGGCTGGTGTACGCCGTGCCGCGACGGTTTGCCGTGGGGGCTGAAGATCCTGCGTGCGCTGG
AGCGCGGCGAGGGGCAGCCGGGGGATATCGAAACCCTTGAGCAGCTGTGCCGCCTGCTGGCGCCGGGTCATACCTTCTGC
GCCCACGCCCCCGGGGCGGTGGAGCCGCTGCAGAGCGCGATTAAATACTTCCGCGGAGAGTTCGAGGCAGGTATTGCCCG
GGAGACGGTCGGCAACGTTAGCCCCATCCGCGGAATTCAGCCCAATTTACTGAAATCACGCTGGTAA

Upstream 100 bases:

>100_bases
GTCTTGGCAACTGCGACAAGGGGCCGAACATGATGATCGATGACGATCTGCACGATCATCTGACGCCGGAGCGGGCCATC
GAACTGCTGGAGCGCTACAA

Downstream 100 bases:

>100_bases
TTGAATTCCTTCGCCGTTGACGGCGGGGGGATACGGTTTTCTTATTTTGGAAGCATGTCAATGGCAACAATACATGTAGA
CGGCAAAGAGTATGACGTCG

Product: NADH dehydrogenase I subunit F

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F; NDH-1 subunit F; NUO6 [H]

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MREIIRTVESHPLTWRLRDDQQPVWLEEYRAKNGYHAAERALKGMAQDEVVTLVKDAGLKGRGGAGFSTGLKWSLMPKDE
SMNVRYLLCNADEMEPGTYKDRLLMEQLPHLLVEGMLIGAYALKAYRGYIFLRGEYVRAAENLRRAIDEARAAGLLGKNI
FGSGFDFDLFVHTGAGRYICGEETALINSLEGRRANPRAKPPFPASAGVWGKPTCVNNVETLCNVPAIIEHGVAWYQGLS
AGKSQDAGTKLMGFSGRVKNPGLWELPFGTTAREILEDYAGGMRDGLKLKAWQPGGAGTDFLTEAHLDLPMDFASIGKAG
SRLGTALAMAVDHEINMVSLLCNLESFFARESCGWCTPCRDGLPWGLKILRALERGEGQPGDIETLEQLCRLLAPGHTFC
AHAPGAVEPLQSAIKYFRGEFEAGIARETVGNVSPIRGIQPNLLKSRW

Sequences:

>Translated_448_residues
MREIIRTVESHPLTWRLRDDQQPVWLEEYRAKNGYHAAERALKGMAQDEVVTLVKDAGLKGRGGAGFSTGLKWSLMPKDE
SMNVRYLLCNADEMEPGTYKDRLLMEQLPHLLVEGMLIGAYALKAYRGYIFLRGEYVRAAENLRRAIDEARAAGLLGKNI
FGSGFDFDLFVHTGAGRYICGEETALINSLEGRRANPRAKPPFPASAGVWGKPTCVNNVETLCNVPAIIEHGVAWYQGLS
AGKSQDAGTKLMGFSGRVKNPGLWELPFGTTAREILEDYAGGMRDGLKLKAWQPGGAGTDFLTEAHLDLPMDFASIGKAG
SRLGTALAMAVDHEINMVSLLCNLESFFARESCGWCTPCRDGLPWGLKILRALERGEGQPGDIETLEQLCRLLAPGHTFC
AHAPGAVEPLQSAIKYFRGEFEAGIARETVGNVSPIRGIQPNLLKSRW
>Mature_448_residues
MREIIRTVESHPLTWRLRDDQQPVWLEEYRAKNGYHAAERALKGMAQDEVVTLVKDAGLKGRGGAGFSTGLKWSLMPKDE
SMNVRYLLCNADEMEPGTYKDRLLMEQLPHLLVEGMLIGAYALKAYRGYIFLRGEYVRAAENLRRAIDEARAAGLLGKNI
FGSGFDFDLFVHTGAGRYICGEETALINSLEGRRANPRAKPPFPASAGVWGKPTCVNNVETLCNVPAIIEHGVAWYQGLS
AGKSQDAGTKLMGFSGRVKNPGLWELPFGTTAREILEDYAGGMRDGLKLKAWQPGGAGTDFLTEAHLDLPMDFASIGKAG
SRLGTALAMAVDHEINMVSLLCNLESFFARESCGWCTPCRDGLPWGLKILRALERGEGQPGDIETLEQLCRLLAPGHTFC
AHAPGAVEPLQSAIKYFRGEFEAGIARETVGNVSPIRGIQPNLLKSRW

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI20149568, Length=395, Percent_Identity=44.8101265822785, Blast_Score=328, Evalue=6e-90,
Organism=Homo sapiens, GI260656005, Length=395, Percent_Identity=44.8101265822785, Blast_Score=328, Evalue=8e-90,
Organism=Escherichia coli, GI1788620, Length=448, Percent_Identity=85.2678571428571, Blast_Score=794, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17531727, Length=394, Percent_Identity=42.8934010152284, Blast_Score=306, Evalue=1e-83,
Organism=Drosophila melanogaster, GI19920794, Length=390, Percent_Identity=45.1282051282051, Blast_Score=328, Evalue=3e-90,
Organism=Drosophila melanogaster, GI45550452, Length=380, Percent_Identity=45, Blast_Score=313, Evalue=2e-85,
Organism=Drosophila melanogaster, GI24653819, Length=386, Percent_Identity=34.1968911917098, Blast_Score=228, Evalue=7e-60,
Organism=Drosophila melanogaster, GI24653817, Length=364, Percent_Identity=32.6923076923077, Blast_Score=203, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 49155; Mature: 49155

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: PS00644 COMPLEX1_51K_1 ; PS00645 COMPLEX1_51K_2 ; PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREIIRTVESHPLTWRLRDDQQPVWLEEYRAKNGYHAAERALKGMAQDEVVTLVKDAGLK
CHHHHHHHHCCCEEEEECCCCCCCHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHCCCC
GRGGAGFSTGLKWSLMPKDESMNVRYLLCNADEMEPGTYKDRLLMEQLPHLLVEGMLIGA
CCCCCCCCCCCEEEECCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
YALKAYRGYIFLRGEYVRAAENLRRAIDEARAAGLLGKNIFGSGFDFDLFVHTGAGRYIC
HHHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEEE
GEETALINSLEGRRANPRAKPPFPASAGVWGKPTCVNNVETLCNVPAIIEHGVAWYQGLS
CCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCC
AGKSQDAGTKLMGFSGRVKNPGLWELPFGTTAREILEDYAGGMRDGLKLKAWQPGGAGTD
CCCCCCCCCEEECCCCCCCCCCEEECCCCCHHHHHHHHHCCCHHCCCEEEEECCCCCCCH
FLTEAHLDLPMDFASIGKAGSRLGTALAMAVDHEINMVSLLCNLESFFARESCGWCTPCR
HHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
DGLPWGLKILRALERGEGQPGDIETLEQLCRLLAPGHTFCAHAPGAVEPLQSAIKYFRGE
CCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHH
FEAGIARETVGNVSPIRGIQPNLLKSRW
HHCCHHHHHHCCCCCCCCCCCHHHHCCC
>Mature Secondary Structure
MREIIRTVESHPLTWRLRDDQQPVWLEEYRAKNGYHAAERALKGMAQDEVVTLVKDAGLK
CHHHHHHHHCCCEEEEECCCCCCCHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHCCCC
GRGGAGFSTGLKWSLMPKDESMNVRYLLCNADEMEPGTYKDRLLMEQLPHLLVEGMLIGA
CCCCCCCCCCCEEEECCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
YALKAYRGYIFLRGEYVRAAENLRRAIDEARAAGLLGKNIFGSGFDFDLFVHTGAGRYIC
HHHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEEE
GEETALINSLEGRRANPRAKPPFPASAGVWGKPTCVNNVETLCNVPAIIEHGVAWYQGLS
CCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCC
AGKSQDAGTKLMGFSGRVKNPGLWELPFGTTAREILEDYAGGMRDGLKLKAWQPGGAGTD
CCCCCCCCCEEECCCCCCCCCCEEECCCCCHHHHHHHHHCCCHHCCCEEEEECCCCCCCH
FLTEAHLDLPMDFASIGKAGSRLGTALAMAVDHEINMVSLLCNLESFFARESCGWCTPCR
HHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
DGLPWGLKILRALERGEGQPGDIETLEQLCRLLAPGHTFCAHAPGAVEPLQSAIKYFRGE
CCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHH
FEAGIARETVGNVSPIRGIQPNLLKSRW
HHCCHHHHHHCCCCCCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7690854; 8157582; 9205837; 9278503; 8366049 [H]