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The map label for this gene is nuoE [H]

Identifier: 238920564

GI number: 238920564

Start: 2572258

End: 2572758

Strand: Reverse

Name: nuoE [H]

Synonym: NT01EI_2676

Alternate gene names: 238920564

Gene position: 2572758-2572258 (Counterclockwise)

Preceding gene: 238920565

Following gene: 238920563

Centisome position: 67.49

GC content: 58.68

Gene sequence:

>501_bases
ATGACAGAAAACGTATTGGATAAGGATTCGGTTTTTGTACTCAGCGACAGCGCGCGTGCTGAGATTGAGCATGAGATCCA
ACACTACGAAGATCCGCGTGCCGCCTCGATCGAGGCGCTGAAGATCGTGCAGAAAGAGCACGGGTGGGTACCGGATCCGG
CCATCGACGCCATCGCCGGTGTGCTGGGGATCCCGGCCGCCGATGTCGAAGGGGTGGCGACGTTTTACAGCCAGATCTTC
CGCCAGCCGGTCGGGCGTCACATCGTGCGCTATTGCGACAGCGTGGTATGTCATATCACCGGCTACCAGGGGATCAAGGC
AGCATTGGAGCAGACGCTGGACATCCAGCCGGGCGAAACCACCTTTGATGGCCGTTTTACCCTGCTGCCGACCTGCTGTC
TTGGCAACTGCGACAAGGGGCCGAACATGATGATCGATGACGATCTGCACGATCATCTGACGCCGGAGCGGGCCATCGAA
CTGCTGGAGCGCTACAAATGA

Upstream 100 bases:

>100_bases
TGCAGCAGATCCCGTCGGTGATCCGTGGCTGTCTGGTGTCCGACTTGATCGTGTACCTCGGCAGTATTGATTTTGTTATG
TCAGATGTGGACCGCTAACC

Downstream 100 bases:

>100_bases
GAGAGATTATTCGTACCGTAGAATCTCACCCGCTGACCTGGCGCCTGCGCGATGACCAACAGCCAGTATGGCTGGAGGAG
TATCGCGCCAAGAACGGCTA

Product: hypothetical protein

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E; NDH-1 subunit E; NUO5 [H]

Number of amino acids: Translated: 166; Mature: 165

Protein sequence:

>166_residues
MTENVLDKDSVFVLSDSARAEIEHEIQHYEDPRAASIEALKIVQKEHGWVPDPAIDAIAGVLGIPAADVEGVATFYSQIF
RQPVGRHIVRYCDSVVCHITGYQGIKAALEQTLDIQPGETTFDGRFTLLPTCCLGNCDKGPNMMIDDDLHDHLTPERAIE
LLERYK

Sequences:

>Translated_166_residues
MTENVLDKDSVFVLSDSARAEIEHEIQHYEDPRAASIEALKIVQKEHGWVPDPAIDAIAGVLGIPAADVEGVATFYSQIF
RQPVGRHIVRYCDSVVCHITGYQGIKAALEQTLDIQPGETTFDGRFTLLPTCCLGNCDKGPNMMIDDDLHDHLTPERAIE
LLERYK
>Mature_165_residues
TENVLDKDSVFVLSDSARAEIEHEIQHYEDPRAASIEALKIVQKEHGWVPDPAIDAIAGVLGIPAADVEGVATFYSQIFR
QPVGRHIVRYCDSVVCHITGYQGIKAALEQTLDIQPGETTFDGRFTLLPTCCLGNCDKGPNMMIDDDLHDHLTPERAIEL
LERYK

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI222080062, Length=162, Percent_Identity=32.0987654320988, Blast_Score=98, Evalue=4e-21,
Organism=Escherichia coli, GI1788621, Length=166, Percent_Identity=77.710843373494, Blast_Score=273, Evalue=4e-75,
Organism=Caenorhabditis elegans, GI17561328, Length=164, Percent_Identity=35.3658536585366, Blast_Score=107, Evalue=2e-24,
Organism=Drosophila melanogaster, GI18859877, Length=155, Percent_Identity=31.6129032258064, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21355353, Length=121, Percent_Identity=32.2314049586777, Blast_Score=83, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 18462; Mature: 18331

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS01099 COMPLEX1_24K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTENVLDKDSVFVLSDSARAEIEHEIQHYEDPRAASIEALKIVQKEHGWVPDPAIDAIAG
CCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHH
VLGIPAADVEGVATFYSQIFRQPVGRHIVRYCDSVVCHITGYQGIKAALEQTLDIQPGET
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCC
TFDGRFTLLPTCCLGNCDKGPNMMIDDDLHDHLTPERAIELLERYK
CCCCCEEEECHHHCCCCCCCCCCEECCCCHHHCCHHHHHHHHHHCC
>Mature Secondary Structure 
TENVLDKDSVFVLSDSARAEIEHEIQHYEDPRAASIEALKIVQKEHGWVPDPAIDAIAG
CCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHH
VLGIPAADVEGVATFYSQIFRQPVGRHIVRYCDSVVCHITGYQGIKAALEQTLDIQPGET
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCC
TFDGRFTLLPTCCLGNCDKGPNMMIDDDLHDHLTPERAIELLERYK
CCCCCEEEECHHHCCCCCCCCCCEECCCCHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]