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The map label for this gene is menH [H]

Identifier: 238920537

GI number: 238920537

Start: 2543241

End: 2544014

Strand: Reverse

Name: menH [H]

Synonym: NT01EI_2649

Alternate gene names: 238920537

Gene position: 2544014-2543241 (Counterclockwise)

Preceding gene: 238920538

Following gene: 238920536

Centisome position: 66.73

GC content: 68.6

Gene sequence:

>774_bases
GTGATCCTGGCCTATCGCCGTCTGGCGGCTGCGTCTCCCGATGCGCCGACCCTGGTATGGCTGCACGGTTTTCTCGGCAG
TCACCGTGAGTGGCTGGCGCAGGCGCAGGCATTGCCACAGTATGCGCATGTGCTGATCGATCTACCGGGGCACGGTGACT
CGGCGGCGCTGACGGTGGACGACTTTGCCCGGTGCGATGCCGCGCTGCGCGATACGCTGCGCGCCGCCGGGGTAACGTCC
TATGGGCTGATCGGCTATTCGCTGGGCGGTCGCCTGGCGATGTATCATGGCTGTCAGGGTGCATCCGGTCTATGGGCGCT
GGCGGTCGAGGGGGGAAACCCCGGACTGGAGACGCCCGCGGAGCGCCAGGCGCGGCAGGCACATGACGGCCGCTGGGCCG
ACCGCCTGCGCAGCGAGCCGCTGGCGCAGGTGCTGGATGATTGGTATCGACAGCCGGTGTTTGCCGATCTGGATGAGTCG
GCGCGGGCCGACCTGGTGTCGCTGCGCGCCGGCCAGCCCGGTGCGGCGCTGGCGCAGATGCTGCAGGCGACCTCGCTGGG
TGTCCAGCCACCGCTGGGCGCGGCGCTGGCGACACTGACCTGTCCGTTATGTTATCTGTGCGGTGAACAGGACACCAAGT
TTCGCACGCTGGCCGGGCGTTGGCGGCTGGCGGTACGTCTGATAGACGGCGCCGGCCACAACGCCCACCGCGCCAACCCG
CGGGCGTTTCGCGACGCGCTGGGAACCTTTTTAACGACATCAACCCCCGGCTGA

Upstream 100 bases:

>100_bases
AGGGCTGGCGGCGTTGCGGGACGACGCTGATCGAACTGCGGGTACCCGGCACGGCGGGCGCGGAGGCGCTGCAGCAGCTG
CTGGGTAAGGTGGCCGCGCT

Downstream 100 bases:

>100_bases
CGCGCCAGACGCGCCGCCGGGTCGGCATCTTTATTAACAGGAAACGTATTATGCTGTATCCAAGCGAACAAGAGCTGTAT
GCCCCGATCGCATGGCAGGA

Product: 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase

Products: NA

Alternate protein names: SHCHC synthase [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MILAYRRLAAASPDAPTLVWLHGFLGSHREWLAQAQALPQYAHVLIDLPGHGDSAALTVDDFARCDAALRDTLRAAGVTS
YGLIGYSLGGRLAMYHGCQGASGLWALAVEGGNPGLETPAERQARQAHDGRWADRLRSEPLAQVLDDWYRQPVFADLDES
ARADLVSLRAGQPGAALAQMLQATSLGVQPPLGAALATLTCPLCYLCGEQDTKFRTLAGRWRLAVRLIDGAGHNAHRANP
RAFRDALGTFLTTSTPG

Sequences:

>Translated_257_residues
MILAYRRLAAASPDAPTLVWLHGFLGSHREWLAQAQALPQYAHVLIDLPGHGDSAALTVDDFARCDAALRDTLRAAGVTS
YGLIGYSLGGRLAMYHGCQGASGLWALAVEGGNPGLETPAERQARQAHDGRWADRLRSEPLAQVLDDWYRQPVFADLDES
ARADLVSLRAGQPGAALAQMLQATSLGVQPPLGAALATLTCPLCYLCGEQDTKFRTLAGRWRLAVRLIDGAGHNAHRANP
RAFRDALGTFLTTSTPG
>Mature_257_residues
MILAYRRLAAASPDAPTLVWLHGFLGSHREWLAQAQALPQYAHVLIDLPGHGDSAALTVDDFARCDAALRDTLRAAGVTS
YGLIGYSLGGRLAMYHGCQGASGLWALAVEGGNPGLETPAERQARQAHDGRWADRLRSEPLAQVLDDWYRQPVFADLDES
ARADLVSLRAGQPGAALAQMLQATSLGVQPPLGAALATLTCPLCYLCGEQDTKFRTLAGRWRLAVRLIDGAGHNAHRANP
RAFRDALGTFLTTSTPG

Specific function: Catalyzes a proton abstraction reaction that results in 2,5-elimination of pyruvate from 2-succinyl-5-enolpyruvyl-6- hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) and the formation of 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate (SHCHC). Is also

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. MenH family [H]

Homologues:

Organism=Escherichia coli, GI1788598, Length=240, Percent_Identity=47.9166666666667, Blast_Score=222, Evalue=2e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR022485 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =4.2.99.20 [H]

Molecular weight: Translated: 27503; Mature: 27503

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILAYRRLAAASPDAPTLVWLHGFLGSHREWLAQAQALPQYAHVLIDLPGHGDSAALTVD
CEECHHHHHCCCCCCCEEEHHHHHHCCHHHHHHHHHHCHHHEEEEEECCCCCCCCEEEHH
DFARCDAALRDTLRAAGVTSYGLIGYSLGGRLAMYHGCQGASGLWALAVEGGNPGLETPA
HHHHHHHHHHHHHHHCCCCHHHHHEECCCCCEEEEECCCCCCCEEEEEEECCCCCCCCCH
ERQARQAHDGRWADRLRSEPLAQVLDDWYRQPVFADLDESARADLVSLRAGQPGAALAQM
HHHHHHHHCCHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHH
LQATSLGVQPPLGAALATLTCPLCYLCGEQDTKFRTLAGRWRLAVRLIDGAGHNAHRANP
HHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHEEEEEEECCCCCCCCCCCH
RAFRDALGTFLTTSTPG
HHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MILAYRRLAAASPDAPTLVWLHGFLGSHREWLAQAQALPQYAHVLIDLPGHGDSAALTVD
CEECHHHHHCCCCCCCEEEHHHHHHCCHHHHHHHHHHCHHHEEEEEECCCCCCCCEEEHH
DFARCDAALRDTLRAAGVTSYGLIGYSLGGRLAMYHGCQGASGLWALAVEGGNPGLETPA
HHHHHHHHHHHHHHHCCCCHHHHHEECCCCCEEEEECCCCCCCEEEEEEECCCCCCCCCH
ERQARQAHDGRWADRLRSEPLAQVLDDWYRQPVFADLDESARADLVSLRAGQPGAALAQM
HHHHHHHHCCHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHH
LQATSLGVQPPLGAALATLTCPLCYLCGEQDTKFRTLAGRWRLAVRLIDGAGHNAHRANP
HHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHEEEEEEECCCCCCCCCCCH
RAFRDALGTFLTTSTPG
HHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA