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The map label for this gene is moaA

Identifier: 238920528

GI number: 238920528

Start: 2532821

End: 2533810

Strand: Reverse

Name: moaA

Synonym: NT01EI_2640

Alternate gene names: 238920528

Gene position: 2533810-2532821 (Counterclockwise)

Preceding gene: 238920530

Following gene: 238920527

Centisome position: 66.46

GC content: 62.53

Gene sequence:

>990_bases
ATGGTGTCCCAGTTAACCGACGCATTTGCGCGCACGTTCTATTATCTGCGCCTGTCAGTCACCGATGTGTGCAACTTTCG
TTGTACCTACTGTTTGCCGGGCGGCTATCGCCCGGATAGCCACGGTGCCAAGCGCTTCTTATCGTGCGATGAGATCCGCC
GTATCAGCCATGCCTTTGCCGCGCTGGGCACCGAAAAGGTGCGTCTGACGGGCGGCGAACCCACCCTACGCCGTGACTTT
TGCGATATCGTCGCCGCTGTGCGTGAGAATTCTGCCATCCGTACGCTGGCGGTGACCACCAACGGTTACCGCATGGCACG
CGACGTCGGCGCCTGGCGTGATGCCGGGCTGACGGCGATCAACGTCAGCGTGGACAGCCTGGATGCCCGCCAGTTTCACG
CCATTACTGGCGAGGATCGCTTCGCTCAGGTGATGGCAGGGATCGATGCGGCCTTTGACGCCGGCTTCGGGCGAATCAAG
GTCAATACCGTGCTGATGCGTGGCGTCAATGACGGCAGCCTGCAGGCCTTTTTGCACTGGATCCGCCGCCGGCCGATTCA
GATGCGCTTTATCGAGCTGATGGAGACCGGCGACGGCAGCGAGCTGTTCCGCCGCCATCACGTGTCCGGCGCGACGATCC
GCACCCAGCTGCTGGCGGCGGGCTGGCAGCGTCGGCTGCCGGGGCGCAGCGACGGTCCGGCGCAGGTCTTCTGGCATCCG
GATTATCTGGGCGAAGTCGGTCTGATCATGCCCTATGAGAAGAACTTTTGCGCCAGCTGCAACCGTCTGCGGGTGTCCGC
CGTGGGTAATCTGCACCTGTGCCTGTTTGGCGAGCAGGGGATCGCTTTGCGCGATCTGCTGCAGGAGGATGCGCAGCAGG
CGGCGTTGCAGGTGCGTATCGCTGCGGCTCTGCGTCATAAGCGGGAGGGCCATTTCCTGCATCAGGGCGACAGTGGCCAT
ACCCAGAATCTTTCTTTCATCGGCGGCTAG

Upstream 100 bases:

>100_bases
AGTGTGCGCTCCCCGCGCAGATAAGGTTTCTTGGCCGTGGCTTCGCGCCACCAGGGCGTCAGGTAGAAATGCCGGCGCCT
CCCGACTTGGAAAGGTGTTT

Downstream 100 bases:

>100_bases
TCCGCCGGGTAGACCCACCTTTTATACAGCGAAGAATGATGAGCCAGTTAACCCATATTAATGCGGCCGGTGAGGCGCAC
ATGGTTGACGTTTCCGCCAA

Product: molybdenum cofactor biosynthesis protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MVSQLTDAFARTFYYLRLSVTDVCNFRCTYCLPGGYRPDSHGAKRFLSCDEIRRISHAFAALGTEKVRLTGGEPTLRRDF
CDIVAAVRENSAIRTLAVTTNGYRMARDVGAWRDAGLTAINVSVDSLDARQFHAITGEDRFAQVMAGIDAAFDAGFGRIK
VNTVLMRGVNDGSLQAFLHWIRRRPIQMRFIELMETGDGSELFRRHHVSGATIRTQLLAAGWQRRLPGRSDGPAQVFWHP
DYLGEVGLIMPYEKNFCASCNRLRVSAVGNLHLCLFGEQGIALRDLLQEDAQQAALQVRIAAALRHKREGHFLHQGDSGH
TQNLSFIGG

Sequences:

>Translated_329_residues
MVSQLTDAFARTFYYLRLSVTDVCNFRCTYCLPGGYRPDSHGAKRFLSCDEIRRISHAFAALGTEKVRLTGGEPTLRRDF
CDIVAAVRENSAIRTLAVTTNGYRMARDVGAWRDAGLTAINVSVDSLDARQFHAITGEDRFAQVMAGIDAAFDAGFGRIK
VNTVLMRGVNDGSLQAFLHWIRRRPIQMRFIELMETGDGSELFRRHHVSGATIRTQLLAAGWQRRLPGRSDGPAQVFWHP
DYLGEVGLIMPYEKNFCASCNRLRVSAVGNLHLCLFGEQGIALRDLLQEDAQQAALQVRIAAALRHKREGHFLHQGDSGH
TQNLSFIGG
>Mature_329_residues
MVSQLTDAFARTFYYLRLSVTDVCNFRCTYCLPGGYRPDSHGAKRFLSCDEIRRISHAFAALGTEKVRLTGGEPTLRRDF
CDIVAAVRENSAIRTLAVTTNGYRMARDVGAWRDAGLTAINVSVDSLDARQFHAITGEDRFAQVMAGIDAAFDAGFGRIK
VNTVLMRGVNDGSLQAFLHWIRRRPIQMRFIELMETGDGSELFRRHHVSGATIRTQLLAAGWQRRLPGRSDGPAQVFWHP
DYLGEVGLIMPYEKNFCASCNRLRVSAVGNLHLCLFGEQGIALRDLLQEDAQQAALQVRIAAALRHKREGHFLHQGDSGH
TQNLSFIGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family

Homologues:

Organism=Homo sapiens, GI116734668, Length=313, Percent_Identity=38.6581469648562, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI22027536, Length=313, Percent_Identity=38.6581469648562, Blast_Score=216, Evalue=3e-56,
Organism=Escherichia coli, GI1786999, Length=329, Percent_Identity=73.5562310030395, Blast_Score=519, Evalue=1e-149,
Organism=Caenorhabditis elegans, GI32566331, Length=308, Percent_Identity=35.7142857142857, Blast_Score=194, Evalue=4e-50,
Organism=Caenorhabditis elegans, GI32566329, Length=308, Percent_Identity=35.7142857142857, Blast_Score=194, Evalue=5e-50,
Organism=Caenorhabditis elegans, GI17561210, Length=308, Percent_Identity=35.7142857142857, Blast_Score=192, Evalue=1e-49,
Organism=Drosophila melanogaster, GI28574488, Length=312, Percent_Identity=36.8589743589744, Blast_Score=206, Evalue=3e-53,
Organism=Drosophila melanogaster, GI28574490, Length=312, Percent_Identity=36.8589743589744, Blast_Score=206, Evalue=3e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MOAA_EDWI9 (C5B7H2)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002934043.1
- ProteinModelPortal:   C5B7H2
- GeneID:   7961666
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_2640
- OMA:   AGWHERP
- ProtClustDB:   PRK00164
- HAMAP:   MF_01225_B
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00729
- TIGRFAMs:   TIGR02666

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM

EC number: NA

Molecular weight: Translated: 36654; Mature: 36654

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: BINDING 17-17 BINDING 30-30 BINDING 68-68 BINDING 72-72 BINDING 99-99 BINDING 123-123 BINDING 160-160 BINDING 194-194

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSQLTDAFARTFYYLRLSVTDVCNFRCTYCLPGGYRPDSHGAKRFLSCDEIRRISHAFA
CCCHHHHHHHHHHEEEEEEEHHHCCCEEEEECCCCCCCCCHHHHHHCCHHHHHHHHHHHH
ALGTEKVRLTGGEPTLRRDFCDIVAAVRENSAIRTLAVTTNGYRMARDVGAWRDAGLTAI
HCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHCCHHHCCEEEE
NVSVDSLDARQFHAITGEDRFAQVMAGIDAAFDAGFGRIKVNTVLMRGVNDGSLQAFLHW
EEEECCCCCHHEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCHHHHHHHH
IRRRPIQMRFIELMETGDGSELFRRHHVSGATIRTQLLAAGWQRRLPGRSDGPAQVFWHP
HHCCCHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEECC
DYLGEVGLIMPYEKNFCASCNRLRVSAVGNLHLCLFGEQGIALRDLLQEDAQQAALQVRI
CCCCCCCEEECCCCCHHCCCCEEEEEEECCEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
AAALRHKREGHFLHQGDSGHTQNLSFIGG
HHHHHHCCCCCEEECCCCCCCCCEEECCC
>Mature Secondary Structure
MVSQLTDAFARTFYYLRLSVTDVCNFRCTYCLPGGYRPDSHGAKRFLSCDEIRRISHAFA
CCCHHHHHHHHHHEEEEEEEHHHCCCEEEEECCCCCCCCCHHHHHHCCHHHHHHHHHHHH
ALGTEKVRLTGGEPTLRRDFCDIVAAVRENSAIRTLAVTTNGYRMARDVGAWRDAGLTAI
HCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHCCHHHCCEEEE
NVSVDSLDARQFHAITGEDRFAQVMAGIDAAFDAGFGRIKVNTVLMRGVNDGSLQAFLHW
EEEECCCCCHHEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCHHHHHHHH
IRRRPIQMRFIELMETGDGSELFRRHHVSGATIRTQLLAAGWQRRLPGRSDGPAQVFWHP
HHCCCHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEECC
DYLGEVGLIMPYEKNFCASCNRLRVSAVGNLHLCLFGEQGIALRDLLQEDAQQAALQVRI
CCCCCCCEEECCCCCHHCCCCEEEEEEECCEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
AAALRHKREGHFLHQGDSGHTQNLSFIGG
HHHHHHCCCCCEEECCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA