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The map label for this gene is eno1 [H]

Identifier: 238920502

GI number: 238920502

Start: 2503862

End: 2504140

Strand: Reverse

Name: eno1 [H]

Synonym: NT01EI_2614

Alternate gene names: 238920502

Gene position: 2504140-2503862 (Counterclockwise)

Preceding gene: 238920503

Following gene: 238920501

Centisome position: 65.69

GC content: 52.69

Gene sequence:

>279_bases
TTGATTAAGCTGAATCAGATCGGCAGCCTGAGTGAGACCATCGCCGCAGTACAGTTGTGTCATAGCCATGGCTGGGGTGC
CTTTATCTTCCACCGCAGTGGTGAAACTGTGGATAGCTTTATCGTCGACATGACTGTTGGACTGCGTGCTGACCACTTGA
AAACCGGTGCATCGTACCGTGGTGAGCGGATCGAAAAATACAATCAGCTGATGTGCATTGAAGATGCGCTGGGGACCGCG
GCGCAGTTTGCCGGTAAACGCGCCTTTGCCCATCTCTAA

Upstream 100 bases:

>100_bases
CCCGCCAGGGACAGCGGATTGAGCGGGTGGGTGACGATCTGTTTGTCACCAACGTACGCTATATCCAGCGCGGTATTGAT
GAGGCGCGGCCAATGCGGCG

Downstream 100 bases:

>100_bases
TCGCGCTTCTCTGCAGCGGCGTGGCGTCAGTCACGCCGCCTTGGGGCGCATTCCCCTTTTTCCCATGTTTTACCATTTCC
CACGGCTTCCCACCCACCTT

Product: hypothetical protein

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase 1; 2-phosphoglycerate dehydratase 1 [H]

Number of amino acids: Translated: 92; Mature: 92

Protein sequence:

>92_residues
MIKLNQIGSLSETIAAVQLCHSHGWGAFIFHRSGETVDSFIVDMTVGLRADHLKTGASYRGERIEKYNQLMCIEDALGTA
AQFAGKRAFAHL

Sequences:

>Translated_92_residues
MIKLNQIGSLSETIAAVQLCHSHGWGAFIFHRSGETVDSFIVDMTVGLRADHLKTGASYRGERIEKYNQLMCIEDALGTA
AQFAGKRAFAHL
>Mature_92_residues
MIKLNQIGSLSETIAAVQLCHSHGWGAFIFHRSGETVDSFIVDMTVGLRADHLKTGASYRGERIEKYNQLMCIEDALGTA
AQFAGKRAFAHL

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI301897477, Length=87, Percent_Identity=56.3218390804598, Blast_Score=107, Evalue=2e-24,
Organism=Homo sapiens, GI301897469, Length=87, Percent_Identity=56.3218390804598, Blast_Score=107, Evalue=2e-24,
Organism=Homo sapiens, GI301897479, Length=87, Percent_Identity=56.3218390804598, Blast_Score=107, Evalue=3e-24,
Organism=Homo sapiens, GI4503571, Length=86, Percent_Identity=52.3255813953488, Blast_Score=104, Evalue=2e-23,
Organism=Homo sapiens, GI5803011, Length=85, Percent_Identity=55.2941176470588, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1789141, Length=87, Percent_Identity=50.5747126436782, Blast_Score=92, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI32563855, Length=85, Percent_Identity=52.9411764705882, Blast_Score=99, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17536383, Length=85, Percent_Identity=52.9411764705882, Blast_Score=99, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI71995829, Length=85, Percent_Identity=52.9411764705882, Blast_Score=99, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6321693, Length=91, Percent_Identity=53.8461538461538, Blast_Score=99, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6323985, Length=87, Percent_Identity=51.7241379310345, Blast_Score=96, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6321968, Length=91, Percent_Identity=51.6483516483517, Blast_Score=96, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324974, Length=87, Percent_Identity=51.7241379310345, Blast_Score=96, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324969, Length=87, Percent_Identity=51.7241379310345, Blast_Score=96, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24580918, Length=86, Percent_Identity=52.3255813953488, Blast_Score=100, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24580916, Length=86, Percent_Identity=52.3255813953488, Blast_Score=100, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24580920, Length=86, Percent_Identity=52.3255813953488, Blast_Score=100, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24580914, Length=86, Percent_Identity=52.3255813953488, Blast_Score=100, Evalue=2e-22,
Organism=Drosophila melanogaster, GI281360527, Length=86, Percent_Identity=52.3255813953488, Blast_Score=100, Evalue=3e-22,
Organism=Drosophila melanogaster, GI17137654, Length=86, Percent_Identity=52.3255813953488, Blast_Score=100, Evalue=3e-22,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 10110; Mature: 10110

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKLNQIGSLSETIAAVQLCHSHGWGAFIFHRSGETVDSFIVDMTVGLRADHLKTGASYR
CEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCCCCHHHCCCCCC
GERIEKYNQLMCIEDALGTAAQFAGKRAFAHL
HHHHHHHHHEEHHHHHHHHHHHHHCCHHHCCC
>Mature Secondary Structure
MIKLNQIGSLSETIAAVQLCHSHGWGAFIFHRSGETVDSFIVDMTVGLRADHLKTGASYR
CEECCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCCCCHHHCCCCCC
GERIEKYNQLMCIEDALGTAAQFAGKRAFAHL
HHHHHHHHHEEHHHHHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901 [H]