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The map label for this gene is rumB [H]

Identifier: 238920414

GI number: 238920414

Start: 2424967

End: 2426100

Strand: Reverse

Name: rumB [H]

Synonym: NT01EI_2524

Alternate gene names: 238920414

Gene position: 2426100-2424967 (Counterclockwise)

Preceding gene: 238920415

Following gene: 238920403

Centisome position: 63.64

GC content: 62.17

Gene sequence:

>1134_bases
ATGCATTGTGTCCAGTATCAGGCGGGCCGCTGTCATTCCTGCCAGTGGCTCTCCATTCCCTATTCCCGTCAGCTGGAGGA
TAAACAGCGGCACCTGTCCGCGCTGCTGAGTGGGCATTCCGTTGCGCAGTGGCTGTCAGCGCAGCCCAGCGCTGAGCTGG
CGATGCGTAACAAGGCGAAAATGGTGGTCAGCGGAAGCGTCGAGCGCCCTCTGCTGGGCATGCTGCAGCGCGACGGCAGT
GGGGTGGATCTGTGCGATTGTCCGCTGTATCCGGACAGCTTTGCCGTCGTCTTCGCCGCGTTGAAGTCCTTTATTCCCCG
TGCCGGTTTGACCCCCTACAGCGTAGCGCGTCGGCGCGGGGAGCTTAAGTATTTGCTGCTTACCGAAAGCCGTCTCAATG
GCGAGATGATGCTGCGCTTTGTGTTGCGCTCAGAGACCAAGCTGGCGCAGCTGCGTCAGGCGCTGCCGTGGCTACAGGCG
CAGCTGCCGCAGCTGGCGGTAATCAGCGTCAATATCCAGCCGGTGCACATGGCCATTCTGGAGGGAGAGCGGGAGATTTT
TCTGACGTCGCAGCAGGTGCTGAGTGAGTCTTTTAACGGCGTGCCGCTCTATATCCGCCCACAGAGCTTCTTTCAGACCA
ACCCCGCGGTGGCTCAGGCGCTGTACGCGCAGGCGCGCGACTGGGTTCAGGCGCTGGGGGTGACGGAAATGTGGGATCTG
TTTTGCGGTGTCGGGGGGTTTGGCCTGCACTGTGCGACCCCGGAGATGACGCTGACCGGCATTGAGATCAGCGCCGGGGC
CATCGCCTGCGCCCGCCGTTCTGCGGCCCAGCTGGGGCTCACGAAGGTAAGCTTCGCCGCCCTGGATTCCGGCGCCTTTG
CCGACGCGCACAGCGCTGTGCCGCAGCTGGTGCTGGTAAACCCGCCGCGGCGCGGTATCGGTGCGGCGCTGTGTGACTAT
ATGAATCGCATGGCGCCGCCCTATATTCTGTACTCCAGCTGCAATGCCCAGAGCATGGTGCAGGATATTGAGCGTCTGGC
CGACTACCGGATCGAAAAGGTCCGTCTGTTCGACATGTTTCCCCATACCGCGCACTATGAGGTCCTTACGCTGCTGGTGC
GCGCATCTGCATAG

Upstream 100 bases:

>100_bases
TATTTATCCCCTCTGCCAGCCGTCGCTACTTCCGGCAGCGGCTGTGATACAATCCGCCACCCCGTCCGTTTCCATTTTCA
TTTCCATCAAGTGAGTTGTG

Downstream 100 bases:

>100_bases
ACCGCCGCCGAACGTCAGGGGCGGTAGGGGATCCCTTCCAGCGCCAGCAGGGCCCGTTTGATGGCCAGTCCGCCGCTGTA
GCCGGTCAGGTTACCGCCCT

Product: 23S rRNA methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U747)-methyltransferase [H]

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MHCVQYQAGRCHSCQWLSIPYSRQLEDKQRHLSALLSGHSVAQWLSAQPSAELAMRNKAKMVVSGSVERPLLGMLQRDGS
GVDLCDCPLYPDSFAVVFAALKSFIPRAGLTPYSVARRRGELKYLLLTESRLNGEMMLRFVLRSETKLAQLRQALPWLQA
QLPQLAVISVNIQPVHMAILEGEREIFLTSQQVLSESFNGVPLYIRPQSFFQTNPAVAQALYAQARDWVQALGVTEMWDL
FCGVGGFGLHCATPEMTLTGIEISAGAIACARRSAAQLGLTKVSFAALDSGAFADAHSAVPQLVLVNPPRRGIGAALCDY
MNRMAPPYILYSSCNAQSMVQDIERLADYRIEKVRLFDMFPHTAHYEVLTLLVRASA

Sequences:

>Translated_377_residues
MHCVQYQAGRCHSCQWLSIPYSRQLEDKQRHLSALLSGHSVAQWLSAQPSAELAMRNKAKMVVSGSVERPLLGMLQRDGS
GVDLCDCPLYPDSFAVVFAALKSFIPRAGLTPYSVARRRGELKYLLLTESRLNGEMMLRFVLRSETKLAQLRQALPWLQA
QLPQLAVISVNIQPVHMAILEGEREIFLTSQQVLSESFNGVPLYIRPQSFFQTNPAVAQALYAQARDWVQALGVTEMWDL
FCGVGGFGLHCATPEMTLTGIEISAGAIACARRSAAQLGLTKVSFAALDSGAFADAHSAVPQLVLVNPPRRGIGAALCDY
MNRMAPPYILYSSCNAQSMVQDIERLADYRIEKVRLFDMFPHTAHYEVLTLLVRASA
>Mature_377_residues
MHCVQYQAGRCHSCQWLSIPYSRQLEDKQRHLSALLSGHSVAQWLSAQPSAELAMRNKAKMVVSGSVERPLLGMLQRDGS
GVDLCDCPLYPDSFAVVFAALKSFIPRAGLTPYSVARRRGELKYLLLTESRLNGEMMLRFVLRSETKLAQLRQALPWLQA
QLPQLAVISVNIQPVHMAILEGEREIFLTSQQVLSESFNGVPLYIRPQSFFQTNPAVAQALYAQARDWVQALGVTEMWDL
FCGVGGFGLHCATPEMTLTGIEISAGAIACARRSAAQLGLTKVSFAALDSGAFADAHSAVPQLVLVNPPRRGIGAALCDY
MNRMAPPYILYSSCNAQSMVQDIERLADYRIEKVRLFDMFPHTAHYEVLTLLVRASA

Specific function: Catalyzes the formation of 5-methyl-uridine at position 747 (M-5-U747) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. RumB subfamily [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=422, Percent_Identity=24.4075829383886, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI51173878, Length=422, Percent_Identity=24.4075829383886, Blast_Score=79, Evalue=7e-15,
Organism=Escherichia coli, GI1787083, Length=374, Percent_Identity=70.3208556149733, Blast_Score=550, Evalue=1e-158,
Organism=Escherichia coli, GI1789148, Length=374, Percent_Identity=25.4010695187166, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790403, Length=187, Percent_Identity=28.3422459893048, Blast_Score=70, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24666579, Length=426, Percent_Identity=25.3521126760563, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011825
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.189 [H]

Molecular weight: Translated: 41676; Mature: 41676

Theoretical pI: Translated: 8.26; Mature: 8.26

Prosite motif: PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHCVQYQAGRCHSCQWLSIPYSRQLEDKQRHLSALLSGHSVAQWLSAQPSAELAMRNKAK
CCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHCCCCE
MVVSGSVERPLLGMLQRDGSGVDLCDCPLYPDSFAVVFAALKSFIPRAGLTPYSVARRRG
EEEECCCCCHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHCC
ELKYLLLTESRLNGEMMLRFVLRSETKLAQLRQALPWLQAQLPQLAVISVNIQPVHMAIL
CEEEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEEEEE
EGEREIFLTSQQVLSESFNGVPLYIRPQSFFQTNPAVAQALYAQARDWVQALGVTEMWDL
CCCCEEEEEHHHHHHHCCCCCEEEECCHHHHCCCHHHHHHHHHHHHHHHHHHCHHHHHHH
FCGVGGFGLHCATPEMTLTGIEISAGAIACARRSAAQLGLTKVSFAALDSGAFADAHSAV
HHCCCCCCEEECCCCEEEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHCC
PQLVLVNPPRRGIGAALCDYMNRMAPPYILYSSCNAQSMVQDIERLADYRIEKVRLFDMF
CEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHEEEEECC
PHTAHYEVLTLLVRASA
CCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MHCVQYQAGRCHSCQWLSIPYSRQLEDKQRHLSALLSGHSVAQWLSAQPSAELAMRNKAK
CCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHCCCCE
MVVSGSVERPLLGMLQRDGSGVDLCDCPLYPDSFAVVFAALKSFIPRAGLTPYSVARRRG
EEEECCCCCHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHCC
ELKYLLLTESRLNGEMMLRFVLRSETKLAQLRQALPWLQAQLPQLAVISVNIQPVHMAIL
CEEEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEEEEE
EGEREIFLTSQQVLSESFNGVPLYIRPQSFFQTNPAVAQALYAQARDWVQALGVTEMWDL
CCCCEEEEEHHHHHHHCCCCCEEEECCHHHHCCCHHHHHHHHHHHHHHHHHHCHHHHHHH
FCGVGGFGLHCATPEMTLTGIEISAGAIACARRSAAQLGLTKVSFAALDSGAFADAHSAV
HHCCCCCCEEECCCCEEEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHCC
PQLVLVNPPRRGIGAALCDYMNRMAPPYILYSSCNAQSMVQDIERLADYRIEKVRLFDMF
CEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHEEEEECC
PHTAHYEVLTLLVRASA
CCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA