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The map label for this gene is ogt [H]

Identifier: 238920413

GI number: 238920413

Start: 2424464

End: 2424952

Strand: Direct

Name: ogt [H]

Synonym: NT01EI_2523

Alternate gene names: 238920413

Gene position: 2424464-2424952 (Clockwise)

Preceding gene: 238920412

Following gene: 238920418

Centisome position: 63.6

GC content: 67.28

Gene sequence:

>489_bases
ATGGACTATGACCACTATGACTCCCCCCTCGGCCCACTGCTGATGGCCGCCGATGGCGGCGGCCTGAGCCAGCTATGGCT
GCCCAATGAGGTGATCGCGCGAGTGATCCCCACCGACTGGCGCCGCTGCGGCGCACATCCCCTGCTGCGGCGCACCCGCG
CCCTGCTCGATGCCTACTTCGCCAAAGAGCGTCCCTGCTGGCAGGATCTGCCACTGACGCCGCAGGGCACCGCCTTTCAG
CGACAGGTATGGCAGCAGCTGAGCGAGGTTCCCTATGGCCAGTGCAGCCACTATGGTGAACTCGCCCGGCGGTTGGACAA
TCCGCGCGCCGTGCGGGCGGTCGGCGGCGCCGTCGGGCGTAATCCGATCGCCATTCTGATCCCCTGCCATCGCATTCTTG
GCAAGGGCGGTAACCTGACCGGCTACAGCGGCGGACTGGCCATCAAACGGGCCCTGCTGGCGCTGGAAGGGATCCCCTAC
CGCCCCTGA

Upstream 100 bases:

>100_bases
GGTTCCCGGCGTCCTGAATGCATCCGGTTGGCGCTCCGGGCGCGATTTGTTAGCATGTCGCCTCCGCCCGTCGGCGCCAC
GCCACGCTTCAGGATACGCT

Downstream 100 bases:

>100_bases
CGTTCGGCGGCGGTCTATGCAGATGCGCGCACCAGCAGCGTAAGGACCTCATAGTGCGCGGTATGGGGAAACATGTCGAA
CAGACGGACCTTTTCGATCC

Product: methylated DNA protein cysteine S-methyltransferase family

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MDYDHYDSPLGPLLMAADGGGLSQLWLPNEVIARVIPTDWRRCGAHPLLRRTRALLDAYFAKERPCWQDLPLTPQGTAFQ
RQVWQQLSEVPYGQCSHYGELARRLDNPRAVRAVGGAVGRNPIAILIPCHRILGKGGNLTGYSGGLAIKRALLALEGIPY
RP

Sequences:

>Translated_162_residues
MDYDHYDSPLGPLLMAADGGGLSQLWLPNEVIARVIPTDWRRCGAHPLLRRTRALLDAYFAKERPCWQDLPLTPQGTAFQ
RQVWQQLSEVPYGQCSHYGELARRLDNPRAVRAVGGAVGRNPIAILIPCHRILGKGGNLTGYSGGLAIKRALLALEGIPY
RP
>Mature_162_residues
MDYDHYDSPLGPLLMAADGGGLSQLWLPNEVIARVIPTDWRRCGAHPLLRRTRALLDAYFAKERPCWQDLPLTPQGTAFQ
RQVWQQLSEVPYGQCSHYGELARRLDNPRAVRAVGGAVGRNPIAILIPCHRILGKGGNLTGYSGGLAIKRALLALEGIPY
RP

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=168, Percent_Identity=37.5, Blast_Score=90, Evalue=9e-19,
Organism=Escherichia coli, GI1787596, Length=102, Percent_Identity=50, Blast_Score=104, Evalue=2e-24,
Organism=Escherichia coli, GI1788542, Length=88, Percent_Identity=47.7272727272727, Blast_Score=86, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI115533070, Length=85, Percent_Identity=47.0588235294118, Blast_Score=87, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI115533068, Length=85, Percent_Identity=47.0588235294118, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6320001, Length=81, Percent_Identity=46.9135802469136, Blast_Score=83, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17137554, Length=105, Percent_Identity=40, Blast_Score=68, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 17908; Mature: 17908

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYDHYDSPLGPLLMAADGGGLSQLWLPNEVIARVIPTDWRRCGAHPLLRRTRALLDAYF
CCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHH
AKERPCWQDLPLTPQGTAFQRQVWQQLSEVPYGQCSHYGELARRLDNPRAVRAVGGAVGR
HCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCC
NPIAILIPCHRILGKGGNLTGYSGGLAIKRALLALEGIPYRP
CCEEEEEECHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDYDHYDSPLGPLLMAADGGGLSQLWLPNEVIARVIPTDWRRCGAHPLLRRTRALLDAYF
CCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHH
AKERPCWQDLPLTPQGTAFQRQVWQQLSEVPYGQCSHYGELARRLDNPRAVRAVGGAVGR
HCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCC
NPIAILIPCHRILGKGGNLTGYSGGLAIKRALLALEGIPYRP
CCEEEEEECHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]