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The map label for this gene is amiD [H]

Identifier: 238920402

GI number: 238920402

Start: 2413333

End: 2414166

Strand: Reverse

Name: amiD [H]

Synonym: NT01EI_2512

Alternate gene names: 238920402

Gene position: 2414166-2413333 (Counterclockwise)

Preceding gene: 238920403

Following gene: 238920398

Centisome position: 63.33

GC content: 62.95

Gene sequence:

>834_bases
ATGAGGGTAAAAACGTCTGTCCTATTGGCGCTATTACTGCTGGTCGGTTGCCAGCAGCCACCGGTGCTGCGCGAGCGCGG
CGGCTATTGGGTGGATGATAGCCAGCGCGCGCTGCGCGCCGAGCCCCGGGTACGCTATCTGGTGCTGCACTATACCGCAG
AGGATTTTCCGGCGTCCTTGCGGGTGCTGATGGGCAAACAGGTCAGCGCACACTATCTGGTCCCCGGCCTGCCGCCGCAG
CGGGACGGCAGGCCGGTGGTGTGGCAGCTGGTGCCAGAGTCGCGTAGCGCCTGGCATGCCGGGGTCAGCTACTGGCGTGG
CCAGACCCACCTCAACGATAACTCGATCGGTATTGAAATTGAAAACCCAGGCTATCGGCGTACGCCGTACGGTATTGTCT
GGTATCCCTTTACTCCTCAGCAGATGACCGCCGTCCGCGCGCTGGCACAGGATATTGTCCGACGCTACGCCATCGCGCCG
CAGAATGTGGTCGGGCATATGGATATTGCGCCACAGCGCAAGGTGGATCCCGGTCCGCTGTTTCCCTGGCAGATGTTGGC
GCAGCAGGGGATCGGCGCCTGGCCGGAGGCAGCGCGGGTCAGGGTCTATCTGCAGCGCTATCGGCAACACCTGCCGGACG
TCGCCTATCTACAGCGGCGGCTGGCACAGTGGGGATATCAGATTGTGCCCAGCGGCATTAATGACGTGGCTTCCCGCCGG
GCCATTGCGGCGTTTCAGATGCACTTCCGCCCGACGGATTTCCGCGGGGAACCGGATGCGGAAACCATTGCTATCGTCGA
TGCGCTGATCGCGCAGTACGGCACCGCCGGTTAA

Upstream 100 bases:

>100_bases
CGATACTCACCTGTGTGGATAGGGAGGGTATCAGCAGCGGGGCTGACGTCGTAGGCCACGGTGGGTAATATCTGCGGTGG
AGAATACGCAAGGAGAAGCG

Downstream 100 bases:

>100_bases
TCGCTCAGCGGTGCTGCAGGTGGCCATGGTCGCGCAGCCAGCGGGCGGTACGCACGATCCCTTCTTCCAGCGTCACGATA
GGCTGATAGCCGAGCTCGCT

Product: N-acetylmuramoyl-L-alanine amidase AmiD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MRVKTSVLLALLLLVGCQQPPVLRERGGYWVDDSQRALRAEPRVRYLVLHYTAEDFPASLRVLMGKQVSAHYLVPGLPPQ
RDGRPVVWQLVPESRSAWHAGVSYWRGQTHLNDNSIGIEIENPGYRRTPYGIVWYPFTPQQMTAVRALAQDIVRRYAIAP
QNVVGHMDIAPQRKVDPGPLFPWQMLAQQGIGAWPEAARVRVYLQRYRQHLPDVAYLQRRLAQWGYQIVPSGINDVASRR
AIAAFQMHFRPTDFRGEPDAETIAIVDALIAQYGTAG

Sequences:

>Translated_277_residues
MRVKTSVLLALLLLVGCQQPPVLRERGGYWVDDSQRALRAEPRVRYLVLHYTAEDFPASLRVLMGKQVSAHYLVPGLPPQ
RDGRPVVWQLVPESRSAWHAGVSYWRGQTHLNDNSIGIEIENPGYRRTPYGIVWYPFTPQQMTAVRALAQDIVRRYAIAP
QNVVGHMDIAPQRKVDPGPLFPWQMLAQQGIGAWPEAARVRVYLQRYRQHLPDVAYLQRRLAQWGYQIVPSGINDVASRR
AIAAFQMHFRPTDFRGEPDAETIAIVDALIAQYGTAG
>Mature_277_residues
MRVKTSVLLALLLLVGCQQPPVLRERGGYWVDDSQRALRAEPRVRYLVLHYTAEDFPASLRVLMGKQVSAHYLVPGLPPQ
RDGRPVVWQLVPESRSAWHAGVSYWRGQTHLNDNSIGIEIENPGYRRTPYGIVWYPFTPQQMTAVRALAQDIVRRYAIAP
QNVVGHMDIAPQRKVDPGPLFPWQMLAQQGIGAWPEAARVRVYLQRYRQHLPDVAYLQRRLAQWGYQIVPSGINDVASRR
AIAAFQMHFRPTDFRGEPDAETIAIVDALIAQYGTAG

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=260, Percent_Identity=55, Blast_Score=281, Evalue=2e-77,
Organism=Escherichia coli, GI1786300, Length=117, Percent_Identity=41.025641025641, Blast_Score=81, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 31426; Mature: 31426

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVKTSVLLALLLLVGCQQPPVLRERGGYWVDDSQRALRAEPRVRYLVLHYTAEDFPASL
CCCHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCHHHHCCCCEEEEEEEECCCCCHHHH
RVLMGKQVSAHYLVPGLPPQRDGRPVVWQLVPESRSAWHAGVSYWRGQTHLNDNSIGIEI
HHHHCCCCCCEEECCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCEEEEE
ENPGYRRTPYGIVWYPFTPQQMTAVRALAQDIVRRYAIAPQNVVGHMDIAPQRKVDPGPL
CCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHEECCCCCCCCCCCCCCC
FPWQMLAQQGIGAWPEAARVRVYLQRYRQHLPDVAYLQRRLAQWGYQIVPSGINDVASRR
CHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEECCCCHHHHHHHH
AIAAFQMHFRPTDFRGEPDAETIAIVDALIAQYGTAG
HHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRVKTSVLLALLLLVGCQQPPVLRERGGYWVDDSQRALRAEPRVRYLVLHYTAEDFPASL
CCCHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCHHHHCCCCEEEEEEEECCCCCHHHH
RVLMGKQVSAHYLVPGLPPQRDGRPVVWQLVPESRSAWHAGVSYWRGQTHLNDNSIGIEI
HHHHCCCCCCEEECCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCEEEEE
ENPGYRRTPYGIVWYPFTPQQMTAVRALAQDIVRRYAIAPQNVVGHMDIAPQRKVDPGPL
CCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHEECCCCCCCCCCCCCCC
FPWQMLAQQGIGAWPEAARVRVYLQRYRQHLPDVAYLQRRLAQWGYQIVPSGINDVASRR
CHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEECCCCHHHHHHHH
AIAAFQMHFRPTDFRGEPDAETIAIVDALIAQYGTAG
HHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]