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The map label for this gene is pflA [H]

Identifier: 238920354

GI number: 238920354

Start: 2359480

End: 2360220

Strand: Direct

Name: pflA [H]

Synonym: NT01EI_2464

Alternate gene names: 238920354

Gene position: 2359480-2360220 (Clockwise)

Preceding gene: 238920353

Following gene: 238920356

Centisome position: 61.89

GC content: 57.49

Gene sequence:

>741_bases
ATGTCAGTAACAGGTCGCATCCACTCGTTCGAATCCTGCGGTACCGTTGACGGCCCAGGCATCCGCTTTATCACCTTCTT
CCAGGGCTGCCTGATGCGCTGCCTGTATTGCCATAACCGCGACACCTGGGATACCCACGGCGGCAAAGAGGTCACCGTAG
AAGAACTGATGAAAGACACCGTCACCTACCGCCACTTTATGAATGCCTCCGGCGGCGGCGTGACGGCCTCCGGCGGCGAA
GCCATGCTGCAGGCCGAGTTTGTGCGCGACTGGTTCCGCGCCTGTCAGGCCGAGGGCATCCACACCTGCCTGGACACCAA
CGGCTTCGTTCGCCGTTACGATCCGGTCATTGATGAGCTGCTGGACGTCACCGACCTGGTGATGCTCGACCTCAAGCAGA
TGAACGACGATATCCATAAGAATCTGGTCGGTGTCTCCAACCATCGCACCCTGGAGTTTTGCCGCTACCTGGCCAAACGC
AACCAGAAAACCTGGATCCGCTACGTGGTGGTCCCGGGCTGGTCAGACGATGAGGCCTCGGCGCGCCTGCTGGGCGAATT
CACCAAGGATATGAAGAATATCGAAAAGATAGAACTGCTGCCCTACCATGAGCTTGGCAAGCACAAGTGGGAAGCGATGG
GGGAAACCTATCAGCTGGGCGACGTGAAGCCCCCCTCCCGCGAGATCATGGAGAAGGTTAAAGGCATTCTGACCGAGTAT
GGTCACAAGGTTATCTATTAA

Upstream 100 bases:

>100_bases
ACAGTTACTACCATCGGGACAGGGCGCTATCCACATATCGGCCTGCCTCATATGGTTTTATGACTGCTATTGATCAGTCA
ATTTTGGAGATAACCCCGCA

Downstream 100 bases:

>100_bases
GCCTGGTACACTCCCGGCGCGACGCCGACGGCGCCGCGTCTCTCTCTGACCCGATAGCCTGCTTAATGCGTCAGCATCTG
CACAAACTGGCGGCTAATAT

Product: pyruvate formate lyase-activating enzyme 1

Products: NA

Alternate protein names: Formate-C-acetyltransferase-activating enzyme 1; PFL-activating enzyme 1 [H]

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MSVTGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWDTHGGKEVTVEELMKDTVTYRHFMNASGGGVTASGGE
AMLQAEFVRDWFRACQAEGIHTCLDTNGFVRRYDPVIDELLDVTDLVMLDLKQMNDDIHKNLVGVSNHRTLEFCRYLAKR
NQKTWIRYVVVPGWSDDEASARLLGEFTKDMKNIEKIELLPYHELGKHKWEAMGETYQLGDVKPPSREIMEKVKGILTEY
GHKVIY

Sequences:

>Translated_246_residues
MSVTGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWDTHGGKEVTVEELMKDTVTYRHFMNASGGGVTASGGE
AMLQAEFVRDWFRACQAEGIHTCLDTNGFVRRYDPVIDELLDVTDLVMLDLKQMNDDIHKNLVGVSNHRTLEFCRYLAKR
NQKTWIRYVVVPGWSDDEASARLLGEFTKDMKNIEKIELLPYHELGKHKWEAMGETYQLGDVKPPSREIMEKVKGILTEY
GHKVIY
>Mature_245_residues
SVTGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWDTHGGKEVTVEELMKDTVTYRHFMNASGGGVTASGGEA
MLQAEFVRDWFRACQAEGIHTCLDTNGFVRRYDPVIDELLDVTDLVMLDLKQMNDDIHKNLVGVSNHRTLEFCRYLAKRN
QKTWIRYVVVPGWSDDEASARLLGEFTKDMKNIEKIELLPYHELGKHKWEAMGETYQLGDVKPPSREIMEKVKGILTEYG
HKVIY

Specific function: Activation of pyruvate formate-lyase 1 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=246, Percent_Identity=85.3658536585366, Blast_Score=451, Evalue=1e-128,
Organism=Escherichia coli, GI1790389, Length=274, Percent_Identity=25.5474452554745, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI226510931, Length=185, Percent_Identity=30.8108108108108, Blast_Score=71, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 28208; Mature: 28076

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVTGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWDTHGGKEVTVEELMKDT
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
VTYRHFMNASGGGVTASGGEAMLQAEFVRDWFRACQAEGIHTCLDTNGFVRRYDPVIDEL
HHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHH
LDVTDLVMLDLKQMNDDIHKNLVGVSNHRTLEFCRYLAKRNQKTWIRYVVVPGWSDDEAS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHH
ARLLGEFTKDMKNIEKIELLPYHELGKHKWEAMGETYQLGDVKPPSREIMEKVKGILTEY
HHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHH
GHKVIY
CCCCCC
>Mature Secondary Structure 
SVTGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWDTHGGKEVTVEELMKDT
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
VTYRHFMNASGGGVTASGGEAMLQAEFVRDWFRACQAEGIHTCLDTNGFVRRYDPVIDEL
HHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHH
LDVTDLVMLDLKQMNDDIHKNLVGVSNHRTLEFCRYLAKRNQKTWIRYVVVPGWSDDEAS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHH
ARLLGEFTKDMKNIEKIELLPYHELGKHKWEAMGETYQLGDVKPPSREIMEKVKGILTEY
HHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHH
GHKVIY
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]