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The map label for this gene is focA [H]

Identifier: 238920352

GI number: 238920352

Start: 2356014

End: 2356871

Strand: Direct

Name: focA [H]

Synonym: NT01EI_2462

Alternate gene names: 238920352

Gene position: 2356014-2356871 (Clockwise)

Preceding gene: 238920351

Following gene: 238920353

Centisome position: 61.8

GC content: 52.33

Gene sequence:

>858_bases
GTGAAAGCTGACAACCCCTTTGACCTACTTTTACCCGCAGCAATGGCCAAAGTGGCCGAAGATGCCGGTGTTTACAAAGC
CACTAAGCACCCGCTAAAAACCTTTTATTTAGCGATCACCGCTGGTGTCTTCATTTCTATCGCCTTCACCTTTTACATCA
CCGTTACGACAGGAACTGCCACTGTTCCGTTTGGCCTGGCCAAGCTGGTGGGCGGTATCTGCTTCTCGCTCGGTTTGATG
CTGGTCGTCGTCTGTGGCGCCGATCTGTTTACCTCTACCGTACTGATCGTGGTCGCCAAAGCCAGCGGCCGTGTCACCTG
GAAACAACTGGGTTTAAACTGGCTGAATGTCTACGTCGGCAATCTGATCGGCGCGCTGTTTTTCGTGGCGCTGATGTGGT
TTGCCGGCGAGTATATGGTTGCAAACGGCGGCTGGGGACTTAATGTGCTCCAAACAGCCGATCACAAGATGCACCACACC
TTTGTTGAAGCCGTCTGCCTGGGCATTCTGGCTAACCTCATGGTCTGTCTGGCTGTATGGATGAGTTACTCCGGACGCAG
TCTGATGGACAAAATGTTTGCCATGGTCCTACCGGTGGCCATGTTTGTTGCCAGTGGCTTTGAGCACAGCATCGCAAACA
TGTTTATGATCCCCTTTGCTATCGTAATCAAAAATTTTGCAACGCCGGAGTTTTGGCAGGCAGTAGGCGCTGCACCGGAA
CAGTTCTCAAACCTGACAGTAAGTCACTTTATTACGGCCAACCTGATCCCGGTCACGCTCGGGAATATCATCGGCGGTGG
CCTGCTGGTGGGTCTGACATACTGGGTAATTTATCTGCGCGGCGGCGATAAGCATTAA

Upstream 100 bases:

>100_bases
TTTGCCTGATTCACTGCCATTCAACATGATCCATGTCAATTTTTGCCGTATACTGCTTTGCTAGTATCTCGGCGCTGATT
AATCTTAAGAGAGAGTTAGT

Downstream 100 bases:

>100_bases
TGTAATGTTTATCCCGAGCAGGGACGGGTTTACAAAGAAATCCACACTAGAAGGTAGGTGTTAAATGACCGAACTTAATG
AAAAACAAGCAGCTGCATGG

Product: formate/nitrite transporter

Products: formate [Periplasm]; formate [Cytoplasm] [C]

Alternate protein names: Formate channel 1 [H]

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MKADNPFDLLLPAAMAKVAEDAGVYKATKHPLKTFYLAITAGVFISIAFTFYITVTTGTATVPFGLAKLVGGICFSLGLM
LVVVCGADLFTSTVLIVVAKASGRVTWKQLGLNWLNVYVGNLIGALFFVALMWFAGEYMVANGGWGLNVLQTADHKMHHT
FVEAVCLGILANLMVCLAVWMSYSGRSLMDKMFAMVLPVAMFVASGFEHSIANMFMIPFAIVIKNFATPEFWQAVGAAPE
QFSNLTVSHFITANLIPVTLGNIIGGGLLVGLTYWVIYLRGGDKH

Sequences:

>Translated_285_residues
MKADNPFDLLLPAAMAKVAEDAGVYKATKHPLKTFYLAITAGVFISIAFTFYITVTTGTATVPFGLAKLVGGICFSLGLM
LVVVCGADLFTSTVLIVVAKASGRVTWKQLGLNWLNVYVGNLIGALFFVALMWFAGEYMVANGGWGLNVLQTADHKMHHT
FVEAVCLGILANLMVCLAVWMSYSGRSLMDKMFAMVLPVAMFVASGFEHSIANMFMIPFAIVIKNFATPEFWQAVGAAPE
QFSNLTVSHFITANLIPVTLGNIIGGGLLVGLTYWVIYLRGGDKH
>Mature_285_residues
MKADNPFDLLLPAAMAKVAEDAGVYKATKHPLKTFYLAITAGVFISIAFTFYITVTTGTATVPFGLAKLVGGICFSLGLM
LVVVCGADLFTSTVLIVVAKASGRVTWKQLGLNWLNVYVGNLIGALFFVALMWFAGEYMVANGGWGLNVLQTADHKMHHT
FVEAVCLGILANLMVCLAVWMSYSGRSLMDKMFAMVLPVAMFVASGFEHSIANMFMIPFAIVIKNFATPEFWQAVGAAPE
QFSNLTVSHFITANLIPVTLGNIIGGGLLVGLTYWVIYLRGGDKH

Specific function: Involved in the bidirectional transport of formate [H]

COG id: COG2116

COG function: function code P; Formate/nitrite family of transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FNT transporter (TC 2.A.44) family [H]

Homologues:

Organism=Escherichia coli, GI1787132, Length=285, Percent_Identity=85.9649122807018, Blast_Score=502, Evalue=1e-143,
Organism=Escherichia coli, GI1788837, Length=280, Percent_Identity=52.8571428571429, Blast_Score=299, Evalue=2e-82,
Organism=Escherichia coli, GI87082249, Length=259, Percent_Identity=29.3436293436293, Blast_Score=75, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6321779, Length=206, Percent_Identity=30.0970873786408, Blast_Score=84, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000292 [H]

Pfam domain/function: PF01226 Form_Nir_trans [H]

EC number: NA

Molecular weight: Translated: 30766; Mature: 30766

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: PS00133 CARBOXYPEPT_ZN_2 ; PS01005 FORMATE_NITRITE_TP_1 ; PS01006 FORMATE_NITRITE_TP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCC
>Mature Secondary Structure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HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate [Cytoplasm]; formate [Periplasm] [C]

Specific reaction: formate [Cytoplasm] = formate [Periplasm] formate [Periplasm] = formate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]