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The map label for this gene is fliA [H]

Identifier: 238920292

GI number: 238920292

Start: 2298743

End: 2299465

Strand: Reverse

Name: fliA [H]

Synonym: NT01EI_2402

Alternate gene names: 238920292

Gene position: 2299465-2298743 (Counterclockwise)

Preceding gene: 238920293

Following gene: 238920291

Centisome position: 60.32

GC content: 60.86

Gene sequence:

>723_bases
GTGACTGATTTGTATACCGCCGAAGGCGTGATTGACAAAAATACGTTATGGCAGCGCTATGCGCCACTGGTTCGTCATGA
GGCGCTGCGTCTGCAGGTTCGCCTTCCGGCCAGCGTAGAGCTGGACGATTTGCTGCAGGCGGGCGGCATCGGGCTGCTGC
ATGCGGTCGAGCGCTATGACGCTATGCAGGGGACTGCATTTACCACCTATGCCATTCAGCGTATCCGCGGTGCGATGCTG
GACGAACTGCGTAGCCGCGACTGGGTGCCGCGCAGTGTGCGGCGGAATGCCCGTGGGGTATCGGCCGCCATGCAGCGCGC
CGAGCAGCGTCTGGGACGGGTGCCGGGTGACAGTGAGATTGCCCAGGAAATGGGGATTTCGCTGGCCGAGTATCAGCAAA
TCCTGCTGGATACCAACACCAGTCAGCTGTTTTCCTATGACGAGTGGCGTGAGGAGCATGGTGACAGTGCTGAGCCGGTC
GTTGAGCAGAGCGAAGAGTCCAACCCATTGCACCAGCTGCTGGAAAGCGATCTGCGTGGCCGGGTGATCGCCGCTATCGA
GGCATTGCCGGAGCGTGAAAAGCTGGTGCTGACCCTGTACTACCAGGAAGAGCTTAACCTCAAGGAGATCGGCGCGGTGC
TGGAGGTCGGTGAATCGCGCGTCAGTCAGCTGCACAGCCAGGCGATTAAACGCCTGCGCACCCGTCTGCTCAGCCAGCGC
TGA

Upstream 100 bases:

>100_bases
AAGCGGCTAAAAGGCCCCGTTTTTACCGAGTTATCGCAGCATTGGATTGCAACAGAGACGGGATAATAAACGGGCATCCA
TCAACCCGGGTTCTGTCGAC

Downstream 100 bases:

>100_bases
GCTGCGTTTTGCCCGAGTATCGGACATGCCCGAATCATCCATTTCTCCGTCTGATAACAGACACCGGCCACTGAGCCGCT
ACCTGAAAGACTTTAAACAT

Product: flagellar biosynthesis sigma factor

Products: NA

Alternate protein names: Sigma-27; Sigma-28; Sigma-F factor [H]

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MTDLYTAEGVIDKNTLWQRYAPLVRHEALRLQVRLPASVELDDLLQAGGIGLLHAVERYDAMQGTAFTTYAIQRIRGAML
DELRSRDWVPRSVRRNARGVSAAMQRAEQRLGRVPGDSEIAQEMGISLAEYQQILLDTNTSQLFSYDEWREEHGDSAEPV
VEQSEESNPLHQLLESDLRGRVIAAIEALPEREKLVLTLYYQEELNLKEIGAVLEVGESRVSQLHSQAIKRLRTRLLSQR

Sequences:

>Translated_240_residues
MTDLYTAEGVIDKNTLWQRYAPLVRHEALRLQVRLPASVELDDLLQAGGIGLLHAVERYDAMQGTAFTTYAIQRIRGAML
DELRSRDWVPRSVRRNARGVSAAMQRAEQRLGRVPGDSEIAQEMGISLAEYQQILLDTNTSQLFSYDEWREEHGDSAEPV
VEQSEESNPLHQLLESDLRGRVIAAIEALPEREKLVLTLYYQEELNLKEIGAVLEVGESRVSQLHSQAIKRLRTRLLSQR
>Mature_239_residues
TDLYTAEGVIDKNTLWQRYAPLVRHEALRLQVRLPASVELDDLLQAGGIGLLHAVERYDAMQGTAFTTYAIQRIRGAMLD
ELRSRDWVPRSVRRNARGVSAAMQRAEQRLGRVPGDSEIAQEMGISLAEYQQILLDTNTSQLFSYDEWREEHGDSAEPVV
EQSEESNPLHQLLESDLRGRVIAAIEALPEREKLVLTLYYQEELNLKEIGAVLEVGESRVSQLHSQAIKRLRTRLLSQR

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is specific for class 3 flagellar operons [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=236, Percent_Identity=79.2372881355932, Blast_Score=381, Evalue=1e-107,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 27305; Mature: 27174

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLYTAEGVIDKNTLWQRYAPLVRHEALRLQVRLPASVELDDLLQAGGIGLLHAVERYD
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHCCHHHHHHHHHHH
AMQGTAFTTYAIQRIRGAMLDELRSRDWVPRSVRRNARGVSAAMQRAEQRLGRVPGDSEI
HHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
AQEMGISLAEYQQILLDTNTSQLFSYDEWREEHGDSAEPVVEQSEESNPLHQLLESDLRG
HHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHCC
RVIAAIEALPEREKLVLTLYYQEELNLKEIGAVLEVGESRVSQLHSQAIKRLRTRLLSQR
HHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TDLYTAEGVIDKNTLWQRYAPLVRHEALRLQVRLPASVELDDLLQAGGIGLLHAVERYD
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHCCHHHHHHHHHHH
AMQGTAFTTYAIQRIRGAMLDELRSRDWVPRSVRRNARGVSAAMQRAEQRLGRVPGDSEI
HHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
AQEMGISLAEYQQILLDTNTSQLFSYDEWREEHGDSAEPVVEQSEESNPLHQLLESDLRG
HHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHCC
RVIAAIEALPEREKLVLTLYYQEELNLKEIGAVLEVGESRVSQLHSQAIKRLRTRLLSQR
HHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]