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The map label for this gene is insB1 [H]

Identifier: 238920250

GI number: 238920250

Start: 2261725

End: 2261961

Strand: Reverse

Name: insB1 [H]

Synonym: NT01EI_2359

Alternate gene names: 238920250

Gene position: 2261961-2261725 (Counterclockwise)

Preceding gene: 238920252

Following gene: 238920249

Centisome position: 59.33

GC content: 45.15

Gene sequence:

>237_bases
GTGAGAAAATTTAATATTGCCTTCTACATGACCGATGCCTGGCCGGTATACCGGACGCTGCTGGATCCAGCTCACCATGT
AGTCAGCAAGAAATATACCCAACGCATAGAACGCCATAACTTGAATCTGCGAACTCATCTCAAACGGCTTACCCGAAGGA
CAATCTGCTTCTCCAAATCAGAAGAAATGCACGATAAGGTCATCGGCTGGTATCTAACAATCAACCATTACCACTAA

Upstream 100 bases:

>100_bases
TCGCTGGCTCTTCTATGCGTATGACCGTATTCGAAAGCGGGTTCTTGCTCATGTCTTTGGCCCTCGAAATGCGTTGACGT
TACAGCGGCTTCTAGCGTTA

Downstream 100 bases:

>100_bases
ATTTGAGTCATGACCAATTCGTGCGTCCGTTTGTCAAAAGTAATAAAAATGATTTTGTTGACGCTGAAGCAATATGTGAA
GCCGCATCCCGACCATCCAT

Product: IS1 ORF

Products: NA

Alternate protein names: IS1a [H]

Number of amino acids: Translated: 78; Mature: 78

Protein sequence:

>78_residues
MRKFNIAFYMTDAWPVYRTLLDPAHHVVSKKYTQRIERHNLNLRTHLKRLTRRTICFSKSEEMHDKVIGWYLTINHYH

Sequences:

>Translated_78_residues
MRKFNIAFYMTDAWPVYRTLLDPAHHVVSKKYTQRIERHNLNLRTHLKRLTRRTICFSKSEEMHDKVIGWYLTINHYH
>Mature_78_residues
MRKFNIAFYMTDAWPVYRTLLDPAHHVVSKKYTQRIERHNLNLRTHLKRLTRRTICFSKSEEMHDKVIGWYLTINHYH

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG1662

COG function: function code L; Transposase and inactivated derivatives, IS1 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 27 family [H]

Homologues:

Organism=Escherichia coli, GI1789853, Length=78, Percent_Identity=58.974358974359, Blast_Score=102, Evalue=3e-24,
Organism=Escherichia coli, GI1788203, Length=78, Percent_Identity=58.974358974359, Blast_Score=102, Evalue=3e-24,
Organism=Escherichia coli, GI1786203, Length=78, Percent_Identity=58.974358974359, Blast_Score=102, Evalue=3e-24,
Organism=Escherichia coli, GI1786470, Length=78, Percent_Identity=58.974358974359, Blast_Score=101, Evalue=7e-24,
Organism=Escherichia coli, GI1786459, Length=78, Percent_Identity=58.974358974359, Blast_Score=101, Evalue=7e-24,
Organism=Escherichia coli, GI1787223, Length=78, Percent_Identity=57.6923076923077, Blast_Score=100, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005063 [H]

Pfam domain/function: PF03400 Transposase_27 [H]

EC number: NA

Molecular weight: Translated: 9628; Mature: 9628

Theoretical pI: Translated: 10.56; Mature: 10.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKFNIAFYMTDAWPVYRTLLDPAHHVVSKKYTQRIERHNLNLRTHLKRLTRRTICFSKS
CCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC
EEMHDKVIGWYLTINHYH
HHHHHHHEEEEEEEECCC
>Mature Secondary Structure
MRKFNIAFYMTDAWPVYRTLLDPAHHVVSKKYTQRIERHNLNLRTHLKRLTRRTICFSKS
CCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCC
EEMHDKVIGWYLTINHYH
HHHHHHHEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 375010; 1849492; 1630901; 9278503 [H]