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The map label for this gene is cobB [H]

Identifier: 238920218

GI number: 238920218

Start: 2225774

End: 2226640

Strand: Reverse

Name: cobB [H]

Synonym: NT01EI_2327

Alternate gene names: 238920218

Gene position: 2226640-2225774 (Counterclockwise)

Preceding gene: 238920219

Following gene: 238920214

Centisome position: 58.41

GC content: 62.17

Gene sequence:

>867_bases
ATGAAAACGCGTCATCGTATTTGTCGCATCAGGAAAAACAAACGGCTACACCATCAGCGGCTGCGCTCTCTGGTCTATTA
TCAGGATATCGTCTGGGGACATGCCGCGGGGGCCGATGCCGATAAGGGGGAGACCATGAAACTGCCTTTCGTAGTGGTAC
TGACGGGGGCCGGTGTGTCGGCAGAATCCGGGATCCGGACTTTCCGCGCGGCCGATGGCCTGTGGGAGGAGCACCGGGTG
GAGGATGTGGCCACACCGGAGGGGTATCGGCGCGAGCCTGCGCTGGTACAGGCGTTCTATAACGCTCGTCGTCATCAGCT
GCAGCAGCCGGCGATCCAGCCCAACCCGGCGCACCTGGCGCTGGCGCGTCTGGAACAGGCGCTGGGCGACCGTTTTTTGC
TGGTTACCCAGAATATCGATAACCTGCACGAGCGCGCCGGGAGCCAGCGGGTGATCCATATGCACGGCGAGTTGCTCAAG
GTGCGCTGCACCCAGTCGGGGCAAGTGGTGAGGTGGTTGGGCGATCTGAGCGTGGATGACCGTTGTCACTGTTGCCAGAT
CCCGGCGCCGCTGCGTCCGCACGTGGTATGGTTCGGCGAGATGCCGATCGGGATGGATGAGATCTACCCAGCGCTGCAGC
AGGCGGATGAGTTTATTGCCATCGGCACCTCGGGCCATGTCTATCCCGCCGCCGGATTTGTCCATGAAGCGCGTCTGTGC
GGCGCCCATACGGTGGAGCTGAATCTGGAGCCGAGCCAGGTGGAGAGCGAGTTCGATGAGCGACACTACGGCCCGGCCAG
TCAGCTGGTTCCGCAGTATCTGCAGCAACTGCTGATCCGCTGCGGCTTTCCCTCCGACGGTCAGTAA

Upstream 100 bases:

>100_bases
ATGGAGATGACGGCGGCACGCGCGGCGCCGCTTTTTTACATCTGAGCGACACCCGCTGCATGGCCCGTGTGGCGGCATGC
GCGGATAAGGAGTCATCATA

Downstream 100 bases:

>100_bases
TGCCCGCATCGGCAGCGCCCCCGCTTACGGTCGCGCTGCCTTACGCGTATGTTCAGTCCTGGAAGAACCAGTACCCGCTG
TTAATCAGCCCGGTCAGGTC

Product: NAD-dependent deacetylase

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog [H]

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MKTRHRICRIRKNKRLHHQRLRSLVYYQDIVWGHAAGADADKGETMKLPFVVVLTGAGVSAESGIRTFRAADGLWEEHRV
EDVATPEGYRREPALVQAFYNARRHQLQQPAIQPNPAHLALARLEQALGDRFLLVTQNIDNLHERAGSQRVIHMHGELLK
VRCTQSGQVVRWLGDLSVDDRCHCCQIPAPLRPHVVWFGEMPIGMDEIYPALQQADEFIAIGTSGHVYPAAGFVHEARLC
GAHTVELNLEPSQVESEFDERHYGPASQLVPQYLQQLLIRCGFPSDGQ

Sequences:

>Translated_288_residues
MKTRHRICRIRKNKRLHHQRLRSLVYYQDIVWGHAAGADADKGETMKLPFVVVLTGAGVSAESGIRTFRAADGLWEEHRV
EDVATPEGYRREPALVQAFYNARRHQLQQPAIQPNPAHLALARLEQALGDRFLLVTQNIDNLHERAGSQRVIHMHGELLK
VRCTQSGQVVRWLGDLSVDDRCHCCQIPAPLRPHVVWFGEMPIGMDEIYPALQQADEFIAIGTSGHVYPAAGFVHEARLC
GAHTVELNLEPSQVESEFDERHYGPASQLVPQYLQQLLIRCGFPSDGQ
>Mature_288_residues
MKTRHRICRIRKNKRLHHQRLRSLVYYQDIVWGHAAGADADKGETMKLPFVVVLTGAGVSAESGIRTFRAADGLWEEHRV
EDVATPEGYRREPALVQAFYNARRHQLQQPAIQPNPAHLALARLEQALGDRFLLVTQNIDNLHERAGSQRVIHMHGELLK
VRCTQSGQVVRWLGDLSVDDRCHCCQIPAPLRPHVVWFGEMPIGMDEIYPALQQADEFIAIGTSGHVYPAAGFVHEARLC
GAHTVELNLEPSQVESEFDERHYGPASQLVPQYLQQLLIRCGFPSDGQ

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI300795542, Length=237, Percent_Identity=36.7088607594937, Blast_Score=148, Evalue=6e-36,
Organism=Homo sapiens, GI6912664, Length=251, Percent_Identity=35.4581673306773, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI13787215, Length=240, Percent_Identity=35.8333333333333, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI6912662, Length=240, Percent_Identity=30.4166666666667, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI7657575, Length=204, Percent_Identity=32.3529411764706, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI308199517, Length=233, Percent_Identity=80.2575107296137, Blast_Score=398, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17541892, Length=201, Percent_Identity=31.3432835820896, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71990482, Length=246, Percent_Identity=26.8292682926829, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17567771, Length=119, Percent_Identity=35.2941176470588, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI17137536, Length=123, Percent_Identity=35.7723577235772, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI28571445, Length=224, Percent_Identity=29.9107142857143, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 32495; Mature: 32495

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTRHRICRIRKNKRLHHQRLRSLVYYQDIVWGHAAGADADKGETMKLPFVVVLTGAGVS
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCEEEEEECCCCC
AESGIRTFRAADGLWEEHRVEDVATPEGYRREPALVQAFYNARRHQLQQPAIQPNPAHLA
HHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHH
LARLEQALGDRFLLVTQNIDNLHERAGSQRVIHMHGELLKVRCTQSGQVVRWLGDLSVDD
HHHHHHHHCCEEEEEECCHHHHHHHCCCCEEEEECCCEEEEEECCCCCEEEEECCCCCCC
RCHCCQIPAPLRPHVVWFGEMPIGMDEIYPALQQADEFIAIGTSGHVYPAAGFVHEARLC
CCCEECCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHC
GAHTVELNLEPSQVESEFDERHYGPASQLVPQYLQQLLIRCGFPSDGQ
CCEEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKTRHRICRIRKNKRLHHQRLRSLVYYQDIVWGHAAGADADKGETMKLPFVVVLTGAGVS
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCEEEEEECCCCC
AESGIRTFRAADGLWEEHRVEDVATPEGYRREPALVQAFYNARRHQLQQPAIQPNPAHLA
HHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHH
LARLEQALGDRFLLVTQNIDNLHERAGSQRVIHMHGELLKVRCTQSGQVVRWLGDLSVDD
HHHHHHHHCCEEEEEECCHHHHHHHCCCCEEEEECCCEEEEEECCCCCEEEEECCCCCCC
RCHCCQIPAPLRPHVVWFGEMPIGMDEIYPALQQADEFIAIGTSGHVYPAAGFVHEARLC
CCCEECCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHC
GAHTVELNLEPSQVESEFDERHYGPASQLVPQYLQQLLIRCGFPSDGQ
CCEEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]