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The map label for this gene is nagK [H]

Identifier: 238920219

GI number: 238920219

Start: 2226640

End: 2227596

Strand: Reverse

Name: nagK [H]

Synonym: NT01EI_2328

Alternate gene names: 238920219

Gene position: 2227596-2226640 (Counterclockwise)

Preceding gene: 238920220

Following gene: 238920218

Centisome position: 58.43

GC content: 65.94

Gene sequence:

>957_bases
ATGTATTACGGTTTCGATCTCGGCGGCAGTAAGCTGGCGCTGGGAGTGTATGATGAGAGTCTGAGCCGGGTCGGCTACCG
GCGCGTGGCGACGCCGCGCGAAGATTACGCCGCGCTGCTGGCGGCGCTGGAGACGCTGATCCACGAGGCCGATCGCCGCT
ACGGCACATGCGGCCGCGTCGGGATCGGTATTCCCGGCTTGCAGCGCCCGGACGGCACCCTGTTTTGCGCCAATGTGCCG
GCGGCGATGGATCGCCCGCTGGCCCGGGATCTGGCGGCGCGCGTTGGCCGCGAGGTGCGGGTAGAGAACGATGCCAACTG
TTTTACCCTCTCCGAGGCGTGGGACAGCGCGGCGCAGGCCTACCCAAGCGTGCTGGGGATCATTCTGGGCACCGGCGTCG
GCGGTGGTCTGGCGATCGACGGGCGGGTGTACCGCGGAGCCAACGGCGTCGCCGGCGAGTTTGGCCATCTGCGCTTACCG
CTGGATGCGCTCGATCTGCTGGGCGCCGATACGCCGCGTCTGGTCTGTGGCTGCGGTCAGCGTGGCTGTATGGAGAATTA
CATCTCGGGGCGCGGATTTGCCTGGCTGTACCATTATGTCTATGGCGAGGCGCTGACGGCGCCGCAGATTGTGGCCCGCT
ACCGGGAGGGCGATGCGCGGGCACAGGTTCACGTCGCGCGCTTCGCTGATGTGCTGGCCATCTGTCTGGCCAATCTGTTT
ACCGTCCTCGATCCCCATCTGGTGGTGATCGGCGGCGGGCTGTCTAACTTTAGCGAGCTGTTTGCCCTGCTGGAAACGCG
GATTTCGCGTTACCTGCTGCGCGTCGCCCGGGTGCCGCCGATCATTGCGGCCCGCTATGGAGATGACGGCGGCACGCGCG
GCGCCGCTTTTTTACATCTGAGCGACACCCGCTGCATGGCCCGTGTGGCGGCATGCGCGGATAAGGAGTCATCATAA

Upstream 100 bases:

>100_bases
CTATCGTGCTGAGTCTGCTGGCCAGCTGGTATCCGGCGCGGCGCGCCAGCCGTATCGACCCTGCGCGGGTGTTGAGCGGC
GGTCAGTGATGGGGGCGTCG

Downstream 100 bases:

>100_bases
TGAAAACGCGTCATCGTATTTGTCGCATCAGGAAAAACAAACGGCTACACCATCAGCGGCTGCGCTCTCTGGTCTATTAT
CAGGATATCGTCTGGGGACA

Product: N-acetyl-D-glucosamine kinase

Products: NA

Alternate protein names: GlcNAc kinase [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MYYGFDLGGSKLALGVYDESLSRVGYRRVATPREDYAALLAALETLIHEADRRYGTCGRVGIGIPGLQRPDGTLFCANVP
AAMDRPLARDLAARVGREVRVENDANCFTLSEAWDSAAQAYPSVLGIILGTGVGGGLAIDGRVYRGANGVAGEFGHLRLP
LDALDLLGADTPRLVCGCGQRGCMENYISGRGFAWLYHYVYGEALTAPQIVARYREGDARAQVHVARFADVLAICLANLF
TVLDPHLVVIGGGLSNFSELFALLETRISRYLLRVARVPPIIAARYGDDGGTRGAAFLHLSDTRCMARVAACADKESS

Sequences:

>Translated_318_residues
MYYGFDLGGSKLALGVYDESLSRVGYRRVATPREDYAALLAALETLIHEADRRYGTCGRVGIGIPGLQRPDGTLFCANVP
AAMDRPLARDLAARVGREVRVENDANCFTLSEAWDSAAQAYPSVLGIILGTGVGGGLAIDGRVYRGANGVAGEFGHLRLP
LDALDLLGADTPRLVCGCGQRGCMENYISGRGFAWLYHYVYGEALTAPQIVARYREGDARAQVHVARFADVLAICLANLF
TVLDPHLVVIGGGLSNFSELFALLETRISRYLLRVARVPPIIAARYGDDGGTRGAAFLHLSDTRCMARVAACADKESS
>Mature_318_residues
MYYGFDLGGSKLALGVYDESLSRVGYRRVATPREDYAALLAALETLIHEADRRYGTCGRVGIGIPGLQRPDGTLFCANVP
AAMDRPLARDLAARVGREVRVENDANCFTLSEAWDSAAQAYPSVLGIILGTGVGGGLAIDGRVYRGANGVAGEFGHLRLP
LDALDLLGADTPRLVCGCGQRGCMENYISGRGFAWLYHYVYGEALTAPQIVARYREGDARAQVHVARFADVLAICLANLF
TVLDPHLVVIGGGLSNFSELFALLETRISRYLLRVARVPPIIAARYGDDGGTRGAAFLHLSDTRCMARVAACADKESS

Specific function: Catalyzes the phosphorylation of N-acetyl-D-glucosamine (GlcNAc) derived from cell-wall degradation, yielding GlcNAc-6-P [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family. NagK subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787363, Length=303, Percent_Identity=58.0858085808581, Blast_Score=360, Evalue=1e-101,
Organism=Escherichia coli, GI87081733, Length=302, Percent_Identity=40.3973509933775, Blast_Score=206, Evalue=1e-54,
Organism=Escherichia coli, GI1790522, Length=316, Percent_Identity=26.8987341772152, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI87082230, Length=270, Percent_Identity=29.6296296296296, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.59 [H]

Molecular weight: Translated: 34118; Mature: 34118

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYYGFDLGGSKLALGVYDESLSRVGYRRVATPREDYAALLAALETLIHEADRRYGTCGRV
CEEEEECCCCEEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GIGIPGLQRPDGTLFCANVPAAMDRPLARDLAARVGREVRVENDANCFTLSEAWDSAAQA
CCCCCCCCCCCCCEEEECCCHHHCCHHHHHHHHHCCCEEEECCCCCEEEEHHHHHHHHHH
YPSVLGIILGTGVGGGLAIDGRVYRGANGVAGEFGHLRLPLDALDLLGADTPRLVCGCGQ
HHHHHHHHHHCCCCCCEEECCEEEECCCCCCCCCCCEECCHHHHHHHCCCCCCEEECCCC
RGCMENYISGRGFAWLYHYVYGEALTAPQIVARYREGDARAQVHVARFADVLAICLANLF
CHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
TVLDPHLVVIGGGLSNFSELFALLETRISRYLLRVARVPPIIAARYGDDGGTRGAAFLHL
HHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEE
SDTRCMARVAACADKESS
CCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MYYGFDLGGSKLALGVYDESLSRVGYRRVATPREDYAALLAALETLIHEADRRYGTCGRV
CEEEEECCCCEEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GIGIPGLQRPDGTLFCANVPAAMDRPLARDLAARVGREVRVENDANCFTLSEAWDSAAQA
CCCCCCCCCCCCCEEEECCCHHHCCHHHHHHHHHCCCEEEECCCCCEEEEHHHHHHHHHH
YPSVLGIILGTGVGGGLAIDGRVYRGANGVAGEFGHLRLPLDALDLLGADTPRLVCGCGQ
HHHHHHHHHHCCCCCCEEECCEEEECCCCCCCCCCCEECCHHHHHHHCCCCCCEEECCCC
RGCMENYISGRGFAWLYHYVYGEALTAPQIVARYREGDARAQVHVARFADVLAICLANLF
CHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
TVLDPHLVVIGGGLSNFSELFALLETRISRYLLRVARVPPIIAARYGDDGGTRGAAFLHL
HHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEE
SDTRCMARVAACADKESS
CCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA