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The map label for this gene is cbiF [H]

Identifier: 238920082

GI number: 238920082

Start: 2112437

End: 2113210

Strand: Reverse

Name: cbiF [H]

Synonym: NT01EI_2187

Alternate gene names: 238920082

Gene position: 2113210-2112437 (Counterclockwise)

Preceding gene: 238920083

Following gene: 238920081

Centisome position: 55.43

GC content: 65.5

Gene sequence:

>774_bases
ATGTCTGAGACCTTTGATGCCCGCTGCGTGTGGTTTGTCGGCGCCGGGCCCGGCGATCGTGAGCTGATTACCCTCAAGGG
CTATCGGCTGCTGCAGCAGGCGCAGGTGGTCATCTATGCCGGCTCGCTGATCAACCCTGAGTTACTGGCGTACTGTCCGC
CCGGGGCCGAGTGCCACGACAGCGCGGCGCTGCATCTGGCGCAGATCCTCGATCTGATGGAGGCTGGGGTACGGGCCGGG
AAAACCGTGGTGCGTCTGCAGACGGGCGATCTCTCCCTGTACGGATCCGTACGTGAGCAGGGGGAGGAGCTGACCAGGCG
CGGCATCGCCTGGCAGGTGGTGCCGGGGGTCAGCGCCTTTCTCGGCGCGGCGGCGGAGCTCGGCGTGGAGTACACCGTGC
CCGACGTCGCGCAAAGCCTGATCATTACCCGCCTTGAGGGGCGTACACCGGTGCCGGCGCGTGAGCAGCTGGAGTCGTTC
GCCCGCCATCAGACCTCGATGGCGATTTACCTCTCCGTCCAACGGATCCAGCGGGTGGCGGAGCGGCTGATCGCGGGGGG
CTATCCGGCCACCACGCCGGTGGCGGTTATCTATAAGGCGACCTGGCCGGAGAGCCGCACCGTCCGCGGCACGCTGGCGG
ACATCGGCGACCGGGTCCACGCTGCGGGGATCCGTAAAACGGCGCTGATCCTTGTCGGCAGTTTCCTTGGCGATGAGTAT
CACTACTCAAAGCTCTATGCGGCGGACTTTAGCCATGAATACCGTAAAGCCTGA

Upstream 100 bases:

>100_bases
CAGCAGATACAGACGGCGTCGTGGGTTCCGCTCGGTCAGGGCCACTACTTCAAACCGAATAATCCCGTTTTTGTTATCGC
CTGCCAGAAGGAGGAACATC

Downstream 100 bases:

>100_bases
ATCCATCGCACTGTTTTGCCTGACCCCCGGCGGCGTGCAGCTGGCCAAGCGGCTGGCGACGCGGCTGCCGCTGACCTGCT
TTACCAGCGTGGCACTGTGG

Product: precorrin-4 C11-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 5

Alternate protein names: Cobalt-precorrin-3 methylase [H]

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MSETFDARCVWFVGAGPGDRELITLKGYRLLQQAQVVIYAGSLINPELLAYCPPGAECHDSAALHLAQILDLMEAGVRAG
KTVVRLQTGDLSLYGSVREQGEELTRRGIAWQVVPGVSAFLGAAAELGVEYTVPDVAQSLIITRLEGRTPVPAREQLESF
ARHQTSMAIYLSVQRIQRVAERLIAGGYPATTPVAVIYKATWPESRTVRGTLADIGDRVHAAGIRKTALILVGSFLGDEY
HYSKLYAADFSHEYRKA

Sequences:

>Translated_257_residues
MSETFDARCVWFVGAGPGDRELITLKGYRLLQQAQVVIYAGSLINPELLAYCPPGAECHDSAALHLAQILDLMEAGVRAG
KTVVRLQTGDLSLYGSVREQGEELTRRGIAWQVVPGVSAFLGAAAELGVEYTVPDVAQSLIITRLEGRTPVPAREQLESF
ARHQTSMAIYLSVQRIQRVAERLIAGGYPATTPVAVIYKATWPESRTVRGTLADIGDRVHAAGIRKTALILVGSFLGDEY
HYSKLYAADFSHEYRKA
>Mature_256_residues
SETFDARCVWFVGAGPGDRELITLKGYRLLQQAQVVIYAGSLINPELLAYCPPGAECHDSAALHLAQILDLMEAGVRAGK
TVVRLQTGDLSLYGSVREQGEELTRRGIAWQVVPGVSAFLGAAAELGVEYTVPDVAQSLIITRLEGRTPVPAREQLESFA
RHQTSMAIYLSVQRIQRVAERLIAGGYPATTPVAVIYKATWPESRTVRGTLADIGDRVHAAGIRKTALILVGSFLGDEYH
YSKLYAADFSHEYRKA

Specific function: Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5 [H]

COG id: COG2875

COG function: function code H; Precorrin-4 methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=247, Percent_Identity=32.3886639676113, Blast_Score=105, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6322922, Length=235, Percent_Identity=22.9787234042553, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006362
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.133

Molecular weight: Translated: 28045; Mature: 27914

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETFDARCVWFVGAGPGDRELITLKGYRLLQQAQVVIYAGSLINPELLAYCPPGAECHD
CCCCCCCEEEEEEECCCCCCEEEEEHHHHHHHHCEEEEEECCCCCCCCEEECCCCCCCCH
SAALHLAQILDLMEAGVRAGKTVVRLQTGDLSLYGSVREQGEELTRRGIAWQVVPGVSAF
HHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEEEHHHHHHHHHHHHCCCEEEECCCHHHH
LGAAAELGVEYTVPDVAQSLIITRLEGRTPVPAREQLESFARHQTSMAIYLSVQRIQRVA
HHHHHHCCCEEECHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEHHHHHHHH
ERLIAGGYPATTPVAVIYKATWPESRTVRGTLADIGDRVHAAGIRKTALILVGSFLGDEY
HHHHCCCCCCCCCEEEEEEECCCCCCCEEEHHHHHCCHHHHHHHHHHHHHHHHHHHCCCH
HYSKLYAADFSHEYRKA
HHHEEEECCCCHHHHCC
>Mature Secondary Structure 
SETFDARCVWFVGAGPGDRELITLKGYRLLQQAQVVIYAGSLINPELLAYCPPGAECHD
CCCCCCEEEEEEECCCCCCEEEEEHHHHHHHHCEEEEEECCCCCCCCEEECCCCCCCCH
SAALHLAQILDLMEAGVRAGKTVVRLQTGDLSLYGSVREQGEELTRRGIAWQVVPGVSAF
HHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEEEHHHHHHHHHHHHCCCEEEECCCHHHH
LGAAAELGVEYTVPDVAQSLIITRLEGRTPVPAREQLESFARHQTSMAIYLSVQRIQRVA
HHHHHHCCCEEECHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEHHHHHHHH
ERLIAGGYPATTPVAVIYKATWPESRTVRGTLADIGDRVHAAGIRKTALILVGSFLGDEY
HHHHCCCCCCCCCEEEEEEECCCCCCCEEEHHHHHCCHHHHHHHHHHHHHHHHHHHCCCH
HYSKLYAADFSHEYRKA
HHHEEEECCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 4

Specific reaction: S-adenosyl-L-methionine + precorrin-4 = S-adenosyl-L-homocysteine + precorrin-5

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]