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The map label for this gene is yngG [H]

Identifier: 238920064

GI number: 238920064

Start: 2096879

End: 2097823

Strand: Direct

Name: yngG [H]

Synonym: NT01EI_2169

Alternate gene names: 238920064

Gene position: 2096879-2097823 (Clockwise)

Preceding gene: 238920063

Following gene: 238920065

Centisome position: 55.0

GC content: 67.72

Gene sequence:

>945_bases
ATGATGACCGAAATGCGAATGTGTGACTGGCCGACTCAGGTTAGCCTGACCGAAGTATGCCCGCGCGACGGGCTACAGAA
TATCTCCGCCTGGATCCCGACGCCGGAGAAGCTGGCGCTGATCGACGGCCTGATGGCCAGCGGCCTGAAGCGAATCGAGG
CGACCTCGTTTGTGCATCCGGCGGCGATCCCGCAGATGAAGGATGCCGACATCGTCGCGCGCCATGCGGTGGCGCAGGCC
CACTCGCTGGGGGTGGAGATCGCGGCGCTGGTGCCGAACCTGAAAGGGGCCGAGCGGGCGCTAACCTGCGGGATCACCAA
GGTCAACTACGTGATCTCCGCCAGCCCGCAGCACAACCTGAAGAATATCAACCGGACGCCGCAGGCGTCGCTGACAGAAC
TGCAGACCCTGCGCTGCGCCCTGCCGGAGCTGGAGGTGACGCTGATCGTCGCCACCGCCTTCGGCTGCCCGTTCCAGGGG
GCGGTGCCGATCGCCGACGTGGCGGCGCTGCTGCGGGCCGGGCGCGAGATGGGGATCAGCCGCTTCACGCTGTGCGACAC
CATCGGCGTGGCCAATCCCCGCCAGACCGTAGAGCTGCTGCAGCAGCTGCGCCTGGCGCTGCCGGGGCTCGATCCCGCCC
TGCACATGCACAACACCCACGGCATGGCGCTGGCCAATATCTATGCGGCCATCAGCCAGGGCGTCACCCGCTTCGAAAGC
GCTGCCGGGGGGCTGGGAGGCTGCCCGTTCGCCCCCGGCGCGGCCGGCAACGCGGCGACGGAGGATCTGGTCAACATGCT
GGATCGCATGGGCATTGCCCACGGCGTCGATCTGGGACGACTGCTGCAAGTGGTGGATCGCCTGCAGGGACTGGCACCGG
CGGCGCTGAGCGGCATGCTGTCGCACGCCCGGCGCTACGACGAATTCGAGTTCAGCCAGCCGTAG

Upstream 100 bases:

>100_bases
ACCGCCGAGATCCTGCAGCGCTACCTGGGGCTAAGCGCGCAGGAGATCGAGCGCCTGACACGCCAGGGGGCGCTATGAAC
CACGCGATACGGAGAGCCTG

Downstream 100 bases:

>100_bases
CTTTCCGCGCGGGGGCGGTACGCCCCCGCCCTTTCCGATGGAGGAGATATGGGTAAAACCGTAGCAGAGAAGATCCTGGC
CGCCAAGGCCGGGCTGACAG

Product: 3-hydroxy-3-methylglutaryl-CoA lyase

Products: NA

Alternate protein names: HL; HMG-CoA lyase; 3-hydroxy-3-methylglutarate-CoA lyase [H]

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MMTEMRMCDWPTQVSLTEVCPRDGLQNISAWIPTPEKLALIDGLMASGLKRIEATSFVHPAAIPQMKDADIVARHAVAQA
HSLGVEIAALVPNLKGAERALTCGITKVNYVISASPQHNLKNINRTPQASLTELQTLRCALPELEVTLIVATAFGCPFQG
AVPIADVAALLRAGREMGISRFTLCDTIGVANPRQTVELLQQLRLALPGLDPALHMHNTHGMALANIYAAISQGVTRFES
AAGGLGGCPFAPGAAGNAATEDLVNMLDRMGIAHGVDLGRLLQVVDRLQGLAPAALSGMLSHARRYDEFEFSQP

Sequences:

>Translated_314_residues
MMTEMRMCDWPTQVSLTEVCPRDGLQNISAWIPTPEKLALIDGLMASGLKRIEATSFVHPAAIPQMKDADIVARHAVAQA
HSLGVEIAALVPNLKGAERALTCGITKVNYVISASPQHNLKNINRTPQASLTELQTLRCALPELEVTLIVATAFGCPFQG
AVPIADVAALLRAGREMGISRFTLCDTIGVANPRQTVELLQQLRLALPGLDPALHMHNTHGMALANIYAAISQGVTRFES
AAGGLGGCPFAPGAAGNAATEDLVNMLDRMGIAHGVDLGRLLQVVDRLQGLAPAALSGMLSHARRYDEFEFSQP
>Mature_314_residues
MMTEMRMCDWPTQVSLTEVCPRDGLQNISAWIPTPEKLALIDGLMASGLKRIEATSFVHPAAIPQMKDADIVARHAVAQA
HSLGVEIAALVPNLKGAERALTCGITKVNYVISASPQHNLKNINRTPQASLTELQTLRCALPELEVTLIVATAFGCPFQG
AVPIADVAALLRAGREMGISRFTLCDTIGVANPRQTVELLQQLRLALPGLDPALHMHNTHGMALANIYAAISQGVTRFES
AAGGLGGCPFAPGAAGNAATEDLVNMLDRMGIAHGVDLGRLLQVVDRLQGLAPAALSGMLSHARRYDEFEFSQP

Specific function: Involved in the catabolism of branched amino acids such as leucine (Probable) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMG-CoA lyase family [H]

Homologues:

Organism=Homo sapiens, GI62198232, Length=279, Percent_Identity=39.7849462365591, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI109150422, Length=278, Percent_Identity=36.6906474820144, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI109150427, Length=280, Percent_Identity=36.4285714285714, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI260654708, Length=116, Percent_Identity=44.8275862068966, Blast_Score=99, Evalue=6e-21,
Organism=Escherichia coli, GI1786261, Length=263, Percent_Identity=25.0950570342205, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17510563, Length=272, Percent_Identity=37.8676470588235, Blast_Score=170, Evalue=8e-43,
Organism=Saccharomyces cerevisiae, GI6320019, Length=249, Percent_Identity=24.8995983935743, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320071, Length=233, Percent_Identity=25.3218884120172, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24582381, Length=274, Percent_Identity=40.5109489051095, Blast_Score=192, Evalue=3e-49,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =4.1.3.4 [H]

Molecular weight: Translated: 33423; Mature: 33423

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTEMRMCDWPTQVSLTEVCPRDGLQNISAWIPTPEKLALIDGLMASGLKRIEATSFVHP
CCCCCCCCCCCCCCCHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
AAIPQMKDADIVARHAVAQAHSLGVEIAALVPNLKGAERALTCGITKVNYVISASPQHNL
HHCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHCCEEEEEEEECCCCHHH
KNINRTPQASLTELQTLRCALPELEVTLIVATAFGCPFQGAVPIADVAALLRAGREMGIS
HHCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCH
RFTLCDTIGVANPRQTVELLQQLRLALPGLDPALHMHNTHGMALANIYAAISQGVTRFES
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
AAGGLGGCPFAPGAAGNAATEDLVNMLDRMGIAHGVDLGRLLQVVDRLQGLAPAALSGML
HCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHCCCHHHHHHHH
SHARRYDEFEFSQP
HHHHHCCCCCCCCC
>Mature Secondary Structure
MMTEMRMCDWPTQVSLTEVCPRDGLQNISAWIPTPEKLALIDGLMASGLKRIEATSFVHP
CCCCCCCCCCCCCCCHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
AAIPQMKDADIVARHAVAQAHSLGVEIAALVPNLKGAERALTCGITKVNYVISASPQHNL
HHCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHCCEEEEEEEECCCCHHH
KNINRTPQASLTELQTLRCALPELEVTLIVATAFGCPFQGAVPIADVAALLRAGREMGIS
HHCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCH
RFTLCDTIGVANPRQTVELLQQLRLALPGLDPALHMHNTHGMALANIYAAISQGVTRFES
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
AAGGLGGCPFAPGAAGNAATEDLVNMLDRMGIAHGVDLGRLLQVVDRLQGLAPAALSGML
HCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHCCCHHHHHHHH
SHARRYDEFEFSQP
HHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]