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The map label for this gene is leuC1 [H]

Identifier: 238920065

GI number: 238920065

Start: 2097872

End: 2099137

Strand: Direct

Name: leuC1 [H]

Synonym: NT01EI_2170

Alternate gene names: 238920065

Gene position: 2097872-2099137 (Clockwise)

Preceding gene: 238920064

Following gene: 238920066

Centisome position: 55.03

GC content: 64.69

Gene sequence:

>1266_bases
ATGGGTAAAACCGTAGCAGAGAAGATCCTGGCCGCCAAGGCCGGGCTGACAGAGGTGACGGCCGGTGATGTGGTCTGGGT
CGACGTCGATAAGGCGATGATGGATGACATCCTGGGACCACGGGTGCAGATCGCCGAACAGCTGGAAAGCCTGAAGATGC
CGATCCGCCACCGCGAGCGGGTAGTGGTCATCGCCGATCACTATACGCCGCCCGCCAACGCGCGTCAGGCGGAGATCGTC
AAGTTTACCCGCGACTGGTCTGCCGAACAGGGCATCGATCGCTACTATGAATTTCAGGGGCCGTGCCACCAGATCATGGC
AGAGCAGGGACACGTACTGCCCGGCACCCTGATCCTGGGCACCGACTCCCACACCTGCATGGGCGGCGCGCTGGGGGCAT
TCGCCACTGGCGTCGGCTCCACCGAGATGGTGGGGATCCTGGCCACCGGCAAAACCTGGCTAAAGGTGCCAGAGAGCCAG
CGGGTTGAGTGGAGCGGTCCGCTGCCCCCCGGGGTGATGGCCAAGGATATCGCCCTGAAGACCATCGGCCACATCGGCCA
CGCCGGGGCGACCTATCAGGCCGTGGAGTTTTGCGGCTCCACCTTTGCCGACATGCCTATCGATCAGCGTATGGCCATCG
CCAATATGGCGGTGGAGATGGGGGCCAAAGCCGGGCTAATGTCGGTAGACGCACTGACGGAGGCCTACCTCGCCGCGCAG
GGAATCGGCCGCGACGCCTATACGGTCTATCCCCCGGACGATGACGCCATCTATGTACGCCAGCTGCGCTTTGCGGCACC
GGCGCTGGCTCCGCAGATTGCCTGCCCGCACAACGTCGATAACGTGACCGATATCGACAACGTGGCCGGTACCCGAATCG
ATCAGGCCTATCTCGGATCCTGTACCGGCGGACGCTATAGCGATCTGGCCGCCGCCGCCGACCTACTGCGCGGCAAACGG
ATCGCCCGCGGCGTGCGCCTGCTGGTCTCACCCGCCTCGCAGCAGATCTGGCAGCAGGCCAGCCGCAGCGGTATTCTCAG
CCAGCTGGCGGAGGCCGGTGCCACCATTCTGGCGCCGACCTGCGGGGTGTGTGTCGGGCTGCACTCCGGGCTGCTGGCGG
GCGGTGAAAACTGCATTTCGGCCAGCAACCGCAACTTCATCGGCCGCATGGGCAGCCGCGATGCCAACATCTATCTGGCC
TCTCCGCTCTCCGTGGCCGCCGCTGCCCTGACCGGCAGCATCATTTCACCGCAAACGCTTCTGTGA

Upstream 100 bases:

>100_bases
CATGCTGTCGCACGCCCGGCGCTACGACGAATTCGAGTTCAGCCAGCCGTAGCTTTCCGCGCGGGGGCGGTACGCCCCCG
CCCTTTCCGATGGAGGAGAT

Downstream 100 bases:

>100_bases
GGTCCCCGATGGATACGCTGCTACACGGCAATGTCTGGAAGTTTGGCGACAATATCAATACCGATATCATCTCGCCGCCG
CAGTATATGGAGCTGAGCAT

Product: homoaconitate hydratase family protein, large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1 [H]

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MGKTVAEKILAAKAGLTEVTAGDVVWVDVDKAMMDDILGPRVQIAEQLESLKMPIRHRERVVVIADHYTPPANARQAEIV
KFTRDWSAEQGIDRYYEFQGPCHQIMAEQGHVLPGTLILGTDSHTCMGGALGAFATGVGSTEMVGILATGKTWLKVPESQ
RVEWSGPLPPGVMAKDIALKTIGHIGHAGATYQAVEFCGSTFADMPIDQRMAIANMAVEMGAKAGLMSVDALTEAYLAAQ
GIGRDAYTVYPPDDDAIYVRQLRFAAPALAPQIACPHNVDNVTDIDNVAGTRIDQAYLGSCTGGRYSDLAAAADLLRGKR
IARGVRLLVSPASQQIWQQASRSGILSQLAEAGATILAPTCGVCVGLHSGLLAGGENCISASNRNFIGRMGSRDANIYLA
SPLSVAAAALTGSIISPQTLL

Sequences:

>Translated_421_residues
MGKTVAEKILAAKAGLTEVTAGDVVWVDVDKAMMDDILGPRVQIAEQLESLKMPIRHRERVVVIADHYTPPANARQAEIV
KFTRDWSAEQGIDRYYEFQGPCHQIMAEQGHVLPGTLILGTDSHTCMGGALGAFATGVGSTEMVGILATGKTWLKVPESQ
RVEWSGPLPPGVMAKDIALKTIGHIGHAGATYQAVEFCGSTFADMPIDQRMAIANMAVEMGAKAGLMSVDALTEAYLAAQ
GIGRDAYTVYPPDDDAIYVRQLRFAAPALAPQIACPHNVDNVTDIDNVAGTRIDQAYLGSCTGGRYSDLAAAADLLRGKR
IARGVRLLVSPASQQIWQQASRSGILSQLAEAGATILAPTCGVCVGLHSGLLAGGENCISASNRNFIGRMGSRDANIYLA
SPLSVAAAALTGSIISPQTLL
>Mature_420_residues
GKTVAEKILAAKAGLTEVTAGDVVWVDVDKAMMDDILGPRVQIAEQLESLKMPIRHRERVVVIADHYTPPANARQAEIVK
FTRDWSAEQGIDRYYEFQGPCHQIMAEQGHVLPGTLILGTDSHTCMGGALGAFATGVGSTEMVGILATGKTWLKVPESQR
VEWSGPLPPGVMAKDIALKTIGHIGHAGATYQAVEFCGSTFADMPIDQRMAIANMAVEMGAKAGLMSVDALTEAYLAAQG
IGRDAYTVYPPDDDAIYVRQLRFAAPALAPQIACPHNVDNVTDIDNVAGTRIDQAYLGSCTGGRYSDLAAAADLLRGKRI
ARGVRLLVSPASQQIWQQASRSGILSQLAEAGATILAPTCGVCVGLHSGLLAGGENCISASNRNFIGRMGSRDANIYLAS
PLSVAAAALTGSIISPQTLL

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=366, Percent_Identity=29.2349726775956, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI8659555, Length=454, Percent_Identity=24.8898678414097, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI41352693, Length=380, Percent_Identity=25, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI1786259, Length=465, Percent_Identity=31.8279569892473, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI87081781, Length=360, Percent_Identity=29.1666666666667, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1787531, Length=394, Percent_Identity=26.1421319796954, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI2367097, Length=351, Percent_Identity=25.3561253561254, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI32564738, Length=364, Percent_Identity=28.021978021978, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI25149337, Length=364, Percent_Identity=28.021978021978, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI25149342, Length=302, Percent_Identity=26.158940397351, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17568399, Length=381, Percent_Identity=25.1968503937008, Blast_Score=79, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6320440, Length=451, Percent_Identity=34.589800443459, Blast_Score=214, Evalue=3e-56,
Organism=Saccharomyces cerevisiae, GI6321429, Length=431, Percent_Identity=29.6983758700696, Blast_Score=172, Evalue=7e-44,
Organism=Saccharomyces cerevisiae, GI6323335, Length=363, Percent_Identity=30.3030303030303, Blast_Score=133, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6322261, Length=387, Percent_Identity=29.7157622739018, Blast_Score=131, Evalue=2e-31,
Organism=Drosophila melanogaster, GI28571643, Length=366, Percent_Identity=28.1420765027322, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI281365315, Length=362, Percent_Identity=27.6243093922652, Blast_Score=101, Evalue=8e-22,
Organism=Drosophila melanogaster, GI17864292, Length=362, Percent_Identity=27.6243093922652, Blast_Score=101, Evalue=8e-22,
Organism=Drosophila melanogaster, GI161076999, Length=362, Percent_Identity=27.6243093922652, Blast_Score=101, Evalue=8e-22,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 44440; Mature: 44309

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKTVAEKILAAKAGLTEVTAGDVVWVDVDKAMMDDILGPRVQIAEQLESLKMPIRHRER
CCHHHHHHHHHHHCCCCEECCCCEEEEECCHHHHHHHCCCHHHHHHHHHHHCCCCCCCCC
VVVIADHYTPPANARQAEIVKFTRDWSAEQGIDRYYEFQGPCHQIMAEQGHVLPGTLILG
EEEEEECCCCCCCCCCHHHHHHHHCCCHHHCHHHHHHCCCHHHHHHHHCCCCCCCEEEEE
TDSHTCMGGALGAFATGVGSTEMVGILATGKTWLKVPESQRVEWSGPLPPGVMAKDIALK
CCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCEEECCCCCCEEECCCCCCCCHHHHHHHH
TIGHIGHAGATYQAVEFCGSTFADMPIDQRMAIANMAVEMGAKAGLMSVDALTEAYLAAQ
HHHHCCCCCCHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
GIGRDAYTVYPPDDDAIYVRQLRFAAPALAPQIACPHNVDNVTDIDNVAGTRIDQAYLGS
CCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCCHHHHCC
CTGGRYSDLAAAADLLRGKRIARGVRLLVSPASQQIWQQASRSGILSQLAEAGATILAPT
CCCCCHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHHHCCCEEECCH
CGVCVGLHSGLLAGGENCISASNRNFIGRMGSRDANIYLASPLSVAAAALTGSIISPQTL
HHHHHHHHHHHHCCCCHHHCCCCCCCEECCCCCCCCEEEECHHHHHHHHHHCCCCCCCCC
L
C
>Mature Secondary Structure 
GKTVAEKILAAKAGLTEVTAGDVVWVDVDKAMMDDILGPRVQIAEQLESLKMPIRHRER
CHHHHHHHHHHHCCCCEECCCCEEEEECCHHHHHHHCCCHHHHHHHHHHHCCCCCCCCC
VVVIADHYTPPANARQAEIVKFTRDWSAEQGIDRYYEFQGPCHQIMAEQGHVLPGTLILG
EEEEEECCCCCCCCCCHHHHHHHHCCCHHHCHHHHHHCCCHHHHHHHHCCCCCCCEEEEE
TDSHTCMGGALGAFATGVGSTEMVGILATGKTWLKVPESQRVEWSGPLPPGVMAKDIALK
CCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCEEECCCCCCEEECCCCCCCCHHHHHHHH
TIGHIGHAGATYQAVEFCGSTFADMPIDQRMAIANMAVEMGAKAGLMSVDALTEAYLAAQ
HHHHCCCCCCHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
GIGRDAYTVYPPDDDAIYVRQLRFAAPALAPQIACPHNVDNVTDIDNVAGTRIDQAYLGS
CCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCCHHHHCC
CTGGRYSDLAAAADLLRGKRIARGVRLLVSPASQQIWQQASRSGILSQLAEAGATILAPT
CCCCCHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHHHCCCEEECCH
CGVCVGLHSGLLAGGENCISASNRNFIGRMGSRDANIYLASPLSVAAAALTGSIISPQTL
HHHHHHHHHHHHCCCCHHHCCCCCCCEECCCCCCCCEEEECHHHHHHHHHHCCCCCCCCC
L
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014 [H]