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The map label for this gene is metC [H]

Identifier: 238920041

GI number: 238920041

Start: 2075574

End: 2076743

Strand: Direct

Name: metC [H]

Synonym: NT01EI_2145

Alternate gene names: 238920041

Gene position: 2075574-2076743 (Clockwise)

Preceding gene: 238920037

Following gene: 238920042

Centisome position: 54.44

GC content: 50.68

Gene sequence:

>1170_bases
ATGACGCGAAACCGTAAAACACAACTAATTCATAGTGGTCGAAAGACATATCAGGGCATGACCCCGGTAAACCCACCGGT
AATGCGTGCGAGTACCATTCTTTTCGAGTCATTAGAAATCTGGCGCGATGTCCGCAAGCGTAGAGAAAACGAACGGTTAC
TCAGCTACGGCGCTCGTGGTACGGATAATGCGTTTGCTCTGGAGCAATTAGTCACCGAATTGGAGGGCGGATACCGTGCC
TGGCTGTTCCCTACGGGGCTAGCGGCAATTGCGATGACCATCATCGGCTATGCCCGTTCCGGGGGACACGTTCTGTTTGC
AGATTCGATTTATGAACCGGTCCGGAAAATGGCCAACGCCTTCTTGATCCCCAATAATATCCAATATGATTTTTTTAAAA
CCGACGGGAGTGATTTTGAATCACTACTACGCCCGGAGACCCAACTAATATTCGTAGAGTCACCGGGCTCCTTGCTATAT
GAAATGCTTGATCTGCCTGCTATCTGCCACATTGCCCATCAACGGGGAATTCCTGTTGCAGTGGATAATACCTGGGGATC
TGGGTATCTCTATAATCCGCTGAAGCTGGGTGCCGATGTATCCGTTATTGCCGCGACCAAATACCTGTGCGGCCATTCTG
ATGTCATGATGGGAATTATTGTTGCCAATCAAGATGCATGGGAAAAGATTGGCGCTCTGCCGGAGGTATTGGGCCAGTGC
AGTAGCCCTGATGATGCCGCTTTAGTCTTACGTGGGGCGCGAACTCTAGCCCCCCGCCTTGAGGTTCATGGCCAGTCGGC
CCTGCTCGTTGCCAAATGGCTACAGCAACGTCGCGAAGTCAAAAAAGTATGGTTCCCCGCACTGGAAGATCATCCAGGCC
ATGAGTTGTGGGAACGTGACTGCACGGGAAGTAATGGATTACTGACCGTTGAGTTTAAAACGCAGTACAACACGACGGAC
AGTGATAATTTTATTAATAGCCTAACGCTGTTTGGTATCGGCGCGTCTTGGGGGGGCTTTGAGAGTCTCGTATTACCGGC
CAATGTGCACGGAGCCCGCAGCGCCACCGACTGGAGTACGCACGGCCCATTCGTTCGTCTACATATTGGGCTGGAAGATG
TCAATGACCTTATTAACGATCTAGCACAAGCATTCCGGGTATTTCAATAA

Upstream 100 bases:

>100_bases
CACGTCTCCAACCAAAAGCAGTTTAGTATTTGGGCACGCACCTTTCTATAGGTGTATCTGCATCATTTCATTAATATATT
TATTATAAGGCATGGGCAAA

Downstream 100 bases:

>100_bases
AGACTAAAGCGTTGCCAACAGTGATGGGAGGAAAGCATGTTGGAACTTATTTTAGCAGGACTAGTCACTACACTGGTGGT
GTATCTACTGGCTAAAGGAT

Product: cystathionine beta-lyase

Products: NA

Alternate protein names: CBL; Beta-cystathionase; Cysteine lyase; Osteotoxin [H]

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MTRNRKTQLIHSGRKTYQGMTPVNPPVMRASTILFESLEIWRDVRKRRENERLLSYGARGTDNAFALEQLVTELEGGYRA
WLFPTGLAAIAMTIIGYARSGGHVLFADSIYEPVRKMANAFLIPNNIQYDFFKTDGSDFESLLRPETQLIFVESPGSLLY
EMLDLPAICHIAHQRGIPVAVDNTWGSGYLYNPLKLGADVSVIAATKYLCGHSDVMMGIIVANQDAWEKIGALPEVLGQC
SSPDDAALVLRGARTLAPRLEVHGQSALLVAKWLQQRREVKKVWFPALEDHPGHELWERDCTGSNGLLTVEFKTQYNTTD
SDNFINSLTLFGIGASWGGFESLVLPANVHGARSATDWSTHGPFVRLHIGLEDVNDLINDLAQAFRVFQ

Sequences:

>Translated_389_residues
MTRNRKTQLIHSGRKTYQGMTPVNPPVMRASTILFESLEIWRDVRKRRENERLLSYGARGTDNAFALEQLVTELEGGYRA
WLFPTGLAAIAMTIIGYARSGGHVLFADSIYEPVRKMANAFLIPNNIQYDFFKTDGSDFESLLRPETQLIFVESPGSLLY
EMLDLPAICHIAHQRGIPVAVDNTWGSGYLYNPLKLGADVSVIAATKYLCGHSDVMMGIIVANQDAWEKIGALPEVLGQC
SSPDDAALVLRGARTLAPRLEVHGQSALLVAKWLQQRREVKKVWFPALEDHPGHELWERDCTGSNGLLTVEFKTQYNTTD
SDNFINSLTLFGIGASWGGFESLVLPANVHGARSATDWSTHGPFVRLHIGLEDVNDLINDLAQAFRVFQ
>Mature_388_residues
TRNRKTQLIHSGRKTYQGMTPVNPPVMRASTILFESLEIWRDVRKRRENERLLSYGARGTDNAFALEQLVTELEGGYRAW
LFPTGLAAIAMTIIGYARSGGHVLFADSIYEPVRKMANAFLIPNNIQYDFFKTDGSDFESLLRPETQLIFVESPGSLLYE
MLDLPAICHIAHQRGIPVAVDNTWGSGYLYNPLKLGADVSVIAATKYLCGHSDVMMGIIVANQDAWEKIGALPEVLGQCS
SPDDAALVLRGARTLAPRLEVHGQSALLVAKWLQQRREVKKVWFPALEDHPGHELWERDCTGSNGLLTVEFKTQYNTTDS
DNFINSLTLFGIGASWGGFESLVLPANVHGARSATDWSTHGPFVRLHIGLEDVNDLINDLAQAFRVFQ

Specific function: Also has cytotoxic activity toward osteogenic, osteosarcoma and tracheal cells, in vitro. The chemical basis for cell toxicity might be the formation and subsequent transfer of sulfane-sulfur to proteins, derived via beta-cystathionase cleavage of L-cysti

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=345, Percent_Identity=35.3623188405797, Blast_Score=175, Evalue=7e-44,
Organism=Homo sapiens, GI299473759, Length=255, Percent_Identity=39.6078431372549, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI25453487, Length=339, Percent_Identity=31.5634218289086, Blast_Score=128, Evalue=7e-30,
Organism=Escherichia coli, GI1789383, Length=391, Percent_Identity=39.8976982097187, Blast_Score=291, Evalue=5e-80,
Organism=Escherichia coli, GI1790375, Length=398, Percent_Identity=32.4120603015075, Blast_Score=174, Evalue=7e-45,
Organism=Caenorhabditis elegans, GI17538055, Length=395, Percent_Identity=31.3924050632911, Blast_Score=174, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI17560056, Length=395, Percent_Identity=29.873417721519, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI71980708, Length=386, Percent_Identity=29.5336787564767, Blast_Score=159, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6321170, Length=334, Percent_Identity=35.9281437125749, Blast_Score=224, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6319307, Length=387, Percent_Identity=29.9741602067183, Blast_Score=145, Evalue=9e-36,
Organism=Saccharomyces cerevisiae, GI6321904, Length=391, Percent_Identity=26.3427109974425, Blast_Score=106, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6323333, Length=353, Percent_Identity=28.328611898017, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6321254, Length=416, Percent_Identity=25, Blast_Score=93, Evalue=8e-20,
Organism=Drosophila melanogaster, GI19922554, Length=391, Percent_Identity=31.9693094629156, Blast_Score=179, Evalue=3e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006233
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.8 [H]

Molecular weight: Translated: 43291; Mature: 43160

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRNRKTQLIHSGRKTYQGMTPVNPPVMRASTILFESLEIWRDVRKRRENERLLSYGARG
CCCCHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TDNAFALEQLVTELEGGYRAWLFPTGLAAIAMTIIGYARSGGHVLFADSIYEPVRKMANA
CCCHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHCE
FLIPNNIQYDFFKTDGSDFESLLRPETQLIFVESPGSLLYEMLDLPAICHIAHQRGIPVA
EECCCCCEEEEEECCCHHHHHHHCCCEEEEEEECCHHHHHHHHCCHHHHHHHHHCCCCEE
VDNTWGSGYLYNPLKLGADVSVIAATKYLCGHSDVMMGIIVANQDAWEKIGALPEVLGQC
EECCCCCCEEECHHHCCCCEEHHHHHHHHCCCCCEEEEEEEECCHHHHHHCCHHHHHHCC
SSPDDAALVLRGARTLAPRLEVHGQSALLVAKWLQQRREVKKVWFPALEDHPGHELWERD
CCCCCCEEEEECCHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHC
CTGSNGLLTVEFKTQYNTTDSDNFINSLTLFGIGASWGGFESLVLPANVHGARSATDWST
CCCCCCEEEEEEEECCCCCCCCHHHHHEEEEEECCCCCCCCEEEEECCCCCCCCCCCCCC
HGPFVRLHIGLEDVNDLINDLAQAFRVFQ
CCCEEEEEECHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TRNRKTQLIHSGRKTYQGMTPVNPPVMRASTILFESLEIWRDVRKRRENERLLSYGARG
CCCHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TDNAFALEQLVTELEGGYRAWLFPTGLAAIAMTIIGYARSGGHVLFADSIYEPVRKMANA
CCCHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHCE
FLIPNNIQYDFFKTDGSDFESLLRPETQLIFVESPGSLLYEMLDLPAICHIAHQRGIPVA
EECCCCCEEEEEECCCHHHHHHHCCCEEEEEEECCHHHHHHHHCCHHHHHHHHHCCCCEE
VDNTWGSGYLYNPLKLGADVSVIAATKYLCGHSDVMMGIIVANQDAWEKIGALPEVLGQC
EECCCCCCEEECHHHCCCCEEHHHHHHHHCCCCCEEEEEEEECCHHHHHHCCHHHHHHCC
SSPDDAALVLRGARTLAPRLEVHGQSALLVAKWLQQRREVKKVWFPALEDHPGHELWERD
CCCCCCEEEEECCHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHC
CTGSNGLLTVEFKTQYNTTDSDNFINSLTLFGIGASWGGFESLVLPANVHGARSATDWST
CCCCCCEEEEEEEECCCCCCCCHHHHHEEEEEECCCCCCCCEEEEECCCCCCCCCCCCCC
HGPFVRLHIGLEDVNDLINDLAQAFRVFQ
CCCEEEEEECHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8463265 [H]