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The map label for this gene is malS [H]

Identifier: 238920023

GI number: 238920023

Start: 2052677

End: 2054710

Strand: Reverse

Name: malS [H]

Synonym: NT01EI_2127

Alternate gene names: 238920023

Gene position: 2054710-2052677 (Counterclockwise)

Preceding gene: 238920024

Following gene: 238920022

Centisome position: 53.9

GC content: 64.65

Gene sequence:

>2034_bases
ATGAAATACGCCATTCTCGCCGCACTATTGTTGCCGGTGGCCGCCGGGGCCGACTGGCGATCGCCCGTGTTTCCCCGGTT
CGCCGCCGTGGGCGAGGGGGTTTTCAGCGCCAGCCAGGCGCTGGCCAAAGGCGAGGCGCCGCTGCAGCTGTATCAGGCCG
ATCGCTGCTGGCAGCCCAGCGGCACGATCCGGCTGAATCAGACGCTCTCGCTGATGCCGTGCCAGGGTGAGGCGCCGCGA
TGGCGGCGCTTTCGGGATGGTCGCTATCAGGCCCGGGTCGATACCCGCCCGGGAACGCCGACGCTGATGCTGTCCGTTCA
GGATGAGGCAGTGCAGCGGGCGGCGGCGCCCGTGCGTCCCTGTCCGCGTTGGAATGGCCGCCCGCTGCGGGTACCGGTCG
ATCAGGCGTTTAGCGAAGGCAGCCGGGTGCGCGATTTTTACAGTGGAGAGGTTGCCCGCGTCACCCAAGGCACGGTGACC
CTGCAGCCGGCGCCGGGCAGCAACGGGCTGCTGCTGCTCGAGTCAGCTGAAACTGCGGGGCCGGCGGCATTCGACTGGCA
CAATGCGACGGTCTATTTTGTCCTGGTCGATCGCTTCGCCAACGGTGATCCCAGCAACGATAATAGCTATGGCCGCCAGC
AGGACGGGATGCAGGAGATCGGGACCTTTCACGGCGGCGATTTGCGCGGGTTGACCGGAAAGCTCGACTATCTCCAGCGT
CTGGGGGTCAATGTCCTGTGGATCAGCTCACCCCTTGAGCAGATCCACGGCTGGGTGGGTGGCGGTAGCCGGGGCGACTT
CCCGCACTATGCCTATCATGGCTACTACCCGCTGGACTGGACGCGGCTGGATGCCAATATGGGCAGCGAGCAGGATCTGC
GGGCGCTGGTGGAGGGGGCGCACCGGCGCGGTATCCGGGTGCTGTTCGATGTGGTGATGAATCATACCGGTTACGCGACG
CTGGCGGATATGCAGCAGTACCGGTTCGGCGCGCTGTTCCTGCAGGGGGAGACGCTGCGGCAAACGCTGGGCACCCATTG
GAGTGACTGGCGGCCGGGGCGGGGTCAGACGTGGCATAGCTTTAATGACTATATTGATTTTGGCAATCGCACGGCGTGGG
CGCGCTGGTGGGGGAAGGGGTGGATCCGTACCGATATCGGCGACTATGACGCCCCGGGATTCGATGATTTTACGCTGTCG
CTCTCTTTTTTGCCGGATGTAAAAACCGAGTCCAGCGTGGCATCCGGTTTGCCGCGCTTTTATCGGCATAAGCCGGATAC
CCGCGCCCGGGAGATCCCAGGCTATACGCCGCGGGACTATCTGACCCGCTGGCTCAGTCAGTGGGTGCGTGAATACGGCA
TCGATGGCTTTCGCATCGATACGGCAAAGCACGTAGAGCTGGCGGCCTGGCAGCAGCTGAAGCAGCAGGCCAGCGCGGCC
TTGGCCGAATGGAAGCGGGCGAATCCGCAGCAGGCGTTGGACGCTGCCCCCTTCTGGATGACCGGCGAGGCCTGGGGGCA
CGGCGTGATGCGCAGCGCCTATTATCGTCACGGTTTCGATGCGATGATCAATTTTGACTATCAGGAGCAGGCGGAGCGGG
CGCAGGCGTGTCTGGCCGATATCGATCCGCTGTGGCAGCAGATGGCCGCGCAGCTGCAGGATTTTAACGTGCTGAGCTAT
CTGTCATCCCATGATACCCACCTATTTCGCAGCGGCGGCAGCCGAGCCGCGGAGCTGCTGCTGCTGGCGCCGGGCGCGGT
GCAGATTTTCTACGGCGATGAGTCGGGGCGTCCGCTGGGGCCCACGGGCTCCGATCCGCTGCAGGGCACCCGTTCGGATA
TGAATTGGCAGGATATCCACGGCCCGGCGGCGGCGCAGGTCGCACACTGGCAGCGCCTGGGGCAGTTCCGCGCCCGTCAT
CCCGCTATCGGCAAAGGGGAGCAGACGACCATCGCGATGCCGCAGGGCTACGGTTTTATCCGCAACGACGGGCGGGATCG
GGTGATGGTGATCTGGGCCGGTCAGCAGGAGTAG

Upstream 100 bases:

>100_bases
CTGGCGTCAGCTCCGCCCCCACCATCCTCCCCCCGGCGGGATGAGCCGCCGGCCCGGCTCCCGTGGCACACTGTGATGGT
CACAGTCACAGGAGCCGATG

Downstream 100 bases:

>100_bases
CGTCAGCTGCGTCAGTTGTTACAAAAATTCTGAGGCGCAGCTCCGGCAACCATTTGATTTTTGCCGGGCCAGTGTGAAAA
GTCGTCGCCATTCGCAGCGT

Product: periplasmic alpha-amylase precursor

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase [H]

Number of amino acids: Translated: 677; Mature: 677

Protein sequence:

>677_residues
MKYAILAALLLPVAAGADWRSPVFPRFAAVGEGVFSASQALAKGEAPLQLYQADRCWQPSGTIRLNQTLSLMPCQGEAPR
WRRFRDGRYQARVDTRPGTPTLMLSVQDEAVQRAAAPVRPCPRWNGRPLRVPVDQAFSEGSRVRDFYSGEVARVTQGTVT
LQPAPGSNGLLLLESAETAGPAAFDWHNATVYFVLVDRFANGDPSNDNSYGRQQDGMQEIGTFHGGDLRGLTGKLDYLQR
LGVNVLWISSPLEQIHGWVGGGSRGDFPHYAYHGYYPLDWTRLDANMGSEQDLRALVEGAHRRGIRVLFDVVMNHTGYAT
LADMQQYRFGALFLQGETLRQTLGTHWSDWRPGRGQTWHSFNDYIDFGNRTAWARWWGKGWIRTDIGDYDAPGFDDFTLS
LSFLPDVKTESSVASGLPRFYRHKPDTRAREIPGYTPRDYLTRWLSQWVREYGIDGFRIDTAKHVELAAWQQLKQQASAA
LAEWKRANPQQALDAAPFWMTGEAWGHGVMRSAYYRHGFDAMINFDYQEQAERAQACLADIDPLWQQMAAQLQDFNVLSY
LSSHDTHLFRSGGSRAAELLLLAPGAVQIFYGDESGRPLGPTGSDPLQGTRSDMNWQDIHGPAAAQVAHWQRLGQFRARH
PAIGKGEQTTIAMPQGYGFIRNDGRDRVMVIWAGQQE

Sequences:

>Translated_677_residues
MKYAILAALLLPVAAGADWRSPVFPRFAAVGEGVFSASQALAKGEAPLQLYQADRCWQPSGTIRLNQTLSLMPCQGEAPR
WRRFRDGRYQARVDTRPGTPTLMLSVQDEAVQRAAAPVRPCPRWNGRPLRVPVDQAFSEGSRVRDFYSGEVARVTQGTVT
LQPAPGSNGLLLLESAETAGPAAFDWHNATVYFVLVDRFANGDPSNDNSYGRQQDGMQEIGTFHGGDLRGLTGKLDYLQR
LGVNVLWISSPLEQIHGWVGGGSRGDFPHYAYHGYYPLDWTRLDANMGSEQDLRALVEGAHRRGIRVLFDVVMNHTGYAT
LADMQQYRFGALFLQGETLRQTLGTHWSDWRPGRGQTWHSFNDYIDFGNRTAWARWWGKGWIRTDIGDYDAPGFDDFTLS
LSFLPDVKTESSVASGLPRFYRHKPDTRAREIPGYTPRDYLTRWLSQWVREYGIDGFRIDTAKHVELAAWQQLKQQASAA
LAEWKRANPQQALDAAPFWMTGEAWGHGVMRSAYYRHGFDAMINFDYQEQAERAQACLADIDPLWQQMAAQLQDFNVLSY
LSSHDTHLFRSGGSRAAELLLLAPGAVQIFYGDESGRPLGPTGSDPLQGTRSDMNWQDIHGPAAAQVAHWQRLGQFRARH
PAIGKGEQTTIAMPQGYGFIRNDGRDRVMVIWAGQQE
>Mature_677_residues
MKYAILAALLLPVAAGADWRSPVFPRFAAVGEGVFSASQALAKGEAPLQLYQADRCWQPSGTIRLNQTLSLMPCQGEAPR
WRRFRDGRYQARVDTRPGTPTLMLSVQDEAVQRAAAPVRPCPRWNGRPLRVPVDQAFSEGSRVRDFYSGEVARVTQGTVT
LQPAPGSNGLLLLESAETAGPAAFDWHNATVYFVLVDRFANGDPSNDNSYGRQQDGMQEIGTFHGGDLRGLTGKLDYLQR
LGVNVLWISSPLEQIHGWVGGGSRGDFPHYAYHGYYPLDWTRLDANMGSEQDLRALVEGAHRRGIRVLFDVVMNHTGYAT
LADMQQYRFGALFLQGETLRQTLGTHWSDWRPGRGQTWHSFNDYIDFGNRTAWARWWGKGWIRTDIGDYDAPGFDDFTLS
LSFLPDVKTESSVASGLPRFYRHKPDTRAREIPGYTPRDYLTRWLSQWVREYGIDGFRIDTAKHVELAAWQQLKQQASAA
LAEWKRANPQQALDAAPFWMTGEAWGHGVMRSAYYRHGFDAMINFDYQEQAERAQACLADIDPLWQQMAAQLQDFNVLSY
LSSHDTHLFRSGGSRAAELLLLAPGAVQIFYGDESGRPLGPTGSDPLQGTRSDMNWQDIHGPAAAQVAHWQRLGQFRARH
PAIGKGEQTTIAMPQGYGFIRNDGRDRVMVIWAGQQE

Specific function: Since only maltooligosaccharides up to a chain length of 6 glucose units are actively transported through the cytoplasmic membrane via the membrane-bound complex of three proteins, MalF, MalG, and MalK, longer maltooligosaccharides must first be degraded

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1789995, Length=677, Percent_Identity=70.0147710487445, Blast_Score=1010, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014635
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =3.2.1.1 [H]

Molecular weight: Translated: 75946; Mature: 75946

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYAILAALLLPVAAGADWRSPVFPRFAAVGEGVFSASQALAKGEAPLQLYQADRCWQPS
CHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCHHHHHHHHHHCCCCCEEEEECCCCCCCC
GTIRLNQTLSLMPCQGEAPRWRRFRDGRYQARVDTRPGTPTLMLSVQDEAVQRAAAPVRP
CCEEECCCEEEEECCCCCCHHHHHCCCCEEEEEECCCCCCEEEEEECHHHHHHHCCCCCC
CPRWNGRPLRVPVDQAFSEGSRVRDFYSGEVARVTQGTVTLQPAPGSNGLLLLESAETAG
CCCCCCCEEEEEHHHHHCCCCHHHHHHCCCEEEEECCEEEEECCCCCCCEEEEECCCCCC
PAAFDWHNATVYFVLVDRFANGDPSNDNSYGRQQDGMQEIGTFHGGDLRGLTGKLDYLQR
CCEEECCCCEEEEEEEEHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHH
LGVNVLWISSPLEQIHGWVGGGSRGDFPHYAYHGYYPLDWTRLDANMGSEQDLRALVEGA
CCCEEEEECCHHHHHCCCCCCCCCCCCCCCEECCCCCCCEEEECCCCCCHHHHHHHHHHH
HRRGIRVLFDVVMNHTGYATLADMQQYRFGALFLQGETLRQTLGTHWSDWRPGRGQTWHS
HHCCHHHHHHHHHCCCCCEEHHHHHHHCEEEEEEECHHHHHHHCCCCCCCCCCCCCCCCC
FNDYIDFGNRTAWARWWGKGWIRTDIGDYDAPGFDDFTLSLSFLPDVKTESSVASGLPRF
CHHHHHCCCCEEEHHHHCCCCEECCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHCCHHH
YRHKPDTRAREIPGYTPRDYLTRWLSQWVREYGIDGFRIDTAKHVELAAWQQLKQQASAA
HCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHH
LAEWKRANPQQALDAAPFWMTGEAWGHGVMRSAYYRHGFDAMINFDYQEQAERAQACLAD
HHHHHHCCHHHHHCCCCCEEECHHHCCHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHH
IDPLWQQMAAQLQDFNVLSYLSSHDTHLFRSGGSRAAELLLLAPGAVQIFYGDESGRPLG
HHHHHHHHHHHHHCCHHHHHHHCCCCHHHHCCCCCEEEEEEECCCEEEEEECCCCCCCCC
PTGSDPLQGTRSDMNWQDIHGPAAAQVAHWQRLGQFRARHPAIGKGEQTTIAMPQGYGFI
CCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCEE
RNDGRDRVMVIWAGQQE
ECCCCCEEEEEECCCCC
>Mature Secondary Structure
MKYAILAALLLPVAAGADWRSPVFPRFAAVGEGVFSASQALAKGEAPLQLYQADRCWQPS
CHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCHHHHHHHHHHCCCCCEEEEECCCCCCCC
GTIRLNQTLSLMPCQGEAPRWRRFRDGRYQARVDTRPGTPTLMLSVQDEAVQRAAAPVRP
CCEEECCCEEEEECCCCCCHHHHHCCCCEEEEEECCCCCCEEEEEECHHHHHHHCCCCCC
CPRWNGRPLRVPVDQAFSEGSRVRDFYSGEVARVTQGTVTLQPAPGSNGLLLLESAETAG
CCCCCCCEEEEEHHHHHCCCCHHHHHHCCCEEEEECCEEEEECCCCCCCEEEEECCCCCC
PAAFDWHNATVYFVLVDRFANGDPSNDNSYGRQQDGMQEIGTFHGGDLRGLTGKLDYLQR
CCEEECCCCEEEEEEEEHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHH
LGVNVLWISSPLEQIHGWVGGGSRGDFPHYAYHGYYPLDWTRLDANMGSEQDLRALVEGA
CCCEEEEECCHHHHHCCCCCCCCCCCCCCCEECCCCCCCEEEECCCCCCHHHHHHHHHHH
HRRGIRVLFDVVMNHTGYATLADMQQYRFGALFLQGETLRQTLGTHWSDWRPGRGQTWHS
HHCCHHHHHHHHHCCCCCEEHHHHHHHCEEEEEEECHHHHHHHCCCCCCCCCCCCCCCCC
FNDYIDFGNRTAWARWWGKGWIRTDIGDYDAPGFDDFTLSLSFLPDVKTESSVASGLPRF
CHHHHHCCCCEEEHHHHCCCCEECCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHCCHHH
YRHKPDTRAREIPGYTPRDYLTRWLSQWVREYGIDGFRIDTAKHVELAAWQQLKQQASAA
HCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHH
LAEWKRANPQQALDAAPFWMTGEAWGHGVMRSAYYRHGFDAMINFDYQEQAERAQACLAD
HHHHHHCCHHHHHCCCCCEEECHHHCCHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHH
IDPLWQQMAAQLQDFNVLSYLSSHDTHLFRSGGSRAAELLLLAPGAVQIFYGDESGRPLG
HHHHHHHHHHHHHCCHHHHHHHCCCCHHHHCCCCCEEEEEEECCCEEEEEECCCCCCCCC
PTGSDPLQGTRSDMNWQDIHGPAAAQVAHWQRLGQFRARHPAIGKGEQTTIAMPQGYGFI
CCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCEE
RNDGRDRVMVIWAGQQE
ECCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1544897; 8041620; 9278503; 9268356 [H]