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The map label for this gene is rnt [H]

Identifier: 238919999

GI number: 238919999

Start: 2024479

End: 2025144

Strand: Direct

Name: rnt [H]

Synonym: NT01EI_2103

Alternate gene names: 238919999

Gene position: 2024479-2025144 (Clockwise)

Preceding gene: 238919998

Following gene: 238920000

Centisome position: 53.1

GC content: 61.11

Gene sequence:

>666_bases
ATGGCTGACACCTCTAATCTCAATGCGCTGAGCACGCGCTTTCGTGGCTTTTATCCGGTCGTCATCGACGTCGAAACCGC
AGGCTTTGACGCCAATCGCAACGCCCTGCTGGAAATTGCCGCCGTCACTCTACGAATGAACGACGACGGCTGGCTGGAAA
CCGATCAGTGCCTGCATTTCCATATCGATCCCTTTGAGGGCGCTGAGCTCTCGCCAGAGGCGCTGGCCTTTAACGGTATC
GACCCACACAACCCGCTGCGCGGCGCCGTCAGCGAATATACCGCGCTGCACGCCATCTTTAAGATGGTGCGCAAGGGAAT
TAAAGATCAGGCGTGCAACCGCGCGGTGGTGGTGGCCCACAACGCCCATTTTGACCACTCCTTTCTGATGGCGGCCGCAG
CGCGCGCCGGGCTGAAGCGTAACCCGTTCCATCCGTTCGCCACCTTTGATACCGCGGCCCTCAGTGGACTGGTTCTCGGC
CAGACGGTGCTGGCCAAGGCCTGCATCAGCGCACAGATCCCCTTCGACAGCGCTCAGGCCCATTCTGCCCTGTATGACAC
CCAGCAGACCGCCACGCTGTTCTGTGAGCTGGTGAATCGCTGGAAGCGCCTGGGCGGCTGGCCCCCGGCCGATCTGAACG
ACACAGGCCTCTGCGCGGAATCATAG

Upstream 100 bases:

>100_bases
TGTACCGCCCACCTCCCTTCTCATTTGCCATCCCCGTGTAAAACACGGCGAAATTTGCCATAATGCGCGCTGCATTCAAA
ATAAAGACACCAACGAACCC

Downstream 100 bases:

>100_bases
TCTCCCTCGCAGACCACGCCCGCTCCGCGGGCGTTTTTTTATTCAGTCCCACGGCGACGCCAAAAAGGCACAGCAGGATA
TTTTTTTGTCGTAGCGCGCA

Product: ribonuclease T

Products: NA

Alternate protein names: Exoribonuclease T; RNase T [H]

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MADTSNLNALSTRFRGFYPVVIDVETAGFDANRNALLEIAAVTLRMNDDGWLETDQCLHFHIDPFEGAELSPEALAFNGI
DPHNPLRGAVSEYTALHAIFKMVRKGIKDQACNRAVVVAHNAHFDHSFLMAAAARAGLKRNPFHPFATFDTAALSGLVLG
QTVLAKACISAQIPFDSAQAHSALYDTQQTATLFCELVNRWKRLGGWPPADLNDTGLCAES

Sequences:

>Translated_221_residues
MADTSNLNALSTRFRGFYPVVIDVETAGFDANRNALLEIAAVTLRMNDDGWLETDQCLHFHIDPFEGAELSPEALAFNGI
DPHNPLRGAVSEYTALHAIFKMVRKGIKDQACNRAVVVAHNAHFDHSFLMAAAARAGLKRNPFHPFATFDTAALSGLVLG
QTVLAKACISAQIPFDSAQAHSALYDTQQTATLFCELVNRWKRLGGWPPADLNDTGLCAES
>Mature_220_residues
ADTSNLNALSTRFRGFYPVVIDVETAGFDANRNALLEIAAVTLRMNDDGWLETDQCLHFHIDPFEGAELSPEALAFNGID
PHNPLRGAVSEYTALHAIFKMVRKGIKDQACNRAVVVAHNAHFDHSFLMAAAARAGLKRNPFHPFATFDTAALSGLVLGQ
TVLAKACISAQIPFDSAQAHSALYDTQQTATLFCELVNRWKRLGGWPPADLNDTGLCAES

Specific function: Responsible for the end-turnover of tRNA:specifically removes the terminal AMP residue from uncharged tRNA (tRNA-C-C-A). Also appears to be involved in tRNA biosynthesis [H]

COG id: COG0847

COG function: function code L; DNA polymerase III, epsilon subunit and related 3'-5' exonucleases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1787941, Length=208, Percent_Identity=74.0384615384615, Blast_Score=331, Evalue=3e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR005987
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00929 Exonuc_X-T [H]

EC number: 3.1.13.-

Molecular weight: Translated: 24055; Mature: 23924

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADTSNLNALSTRFRGFYPVVIDVETAGFDANRNALLEIAAVTLRMNDDGWLETDQCLHF
CCCCCCCHHHHHHHCCEEEEEEEEEECCCCCCCHHHEEEEEEEEEECCCCCCCCCCEEEE
HIDPFEGAELSPEALAFNGIDPHNPLRGAVSEYTALHAIFKMVRKGIKDQACNRAVVVAH
ECCCCCCCCCCCHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHCCCEEEEEE
NAHFDHSFLMAAAARAGLKRNPFHPFATFDTAALSGLVLGQTVLAKACISAQIPFDSAQA
CCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
HSALYDTQQTATLFCELVNRWKRLGGWPPADLNDTGLCAES
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ADTSNLNALSTRFRGFYPVVIDVETAGFDANRNALLEIAAVTLRMNDDGWLETDQCLHF
CCCCCCHHHHHHHCCEEEEEEEEEECCCCCCCHHHEEEEEEEEEECCCCCCCCCCEEEE
HIDPFEGAELSPEALAFNGIDPHNPLRGAVSEYTALHAIFKMVRKGIKDQACNRAVVVAH
ECCCCCCCCCCCHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHCCCEEEEEE
NAHFDHSFLMAAAARAGLKRNPFHPFATFDTAALSGLVLGQTVLAKACISAQIPFDSAQA
CCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
HSALYDTQQTATLFCELVNRWKRLGGWPPADLNDTGLCAES
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA