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The map label for this gene is gloA [H]

Identifier: 238919998

GI number: 238919998

Start: 2023937

End: 2024344

Strand: Direct

Name: gloA [H]

Synonym: NT01EI_2102

Alternate gene names: 238919998

Gene position: 2023937-2024344 (Clockwise)

Preceding gene: 238919996

Following gene: 238919999

Centisome position: 53.09

GC content: 59.56

Gene sequence:

>408_bases
ATGCGTGTACTTCACACCATGCTGCGGGTCGCCGACCTGAAGCGCTCTGTCGATTTTTATACCCAGGTGCTGGGCATGCG
CCTGCTGCGCACCAGTGAAAATGAAGCCTATAAATACTCTCTGGCCTTCGTCGGCTATGGCGATGAGGAGCAAGGCGCCG
TGATCGAACTGACCTATAACTGGGGCGTCGATAGCTATGAGATGGGCAGCGCCTTCGGCCATATCGCCCTCGGCGTCGAT
GACGTCGCCGCCACCGTCGGGCAGATCCGCCGCGCCGGTGGCAACATTACCCGTGAGGCAGGCCCGGTCAAAGGCGGCCA
TACGATCATTGCCTTTGTCGAGGATCCCGACGGCTATAAAATCGAGCTGATCGAAAACCGCTCGGCCAGCGAAGGACTGG
GCCGCTAA

Upstream 100 bases:

>100_bases
CAAACCGCCCTGCCCGCTCACCGGGTTTGACGTTGCCAAATCGGCGGCCTCTGTGACACTATTGAAACGTCTTTTTACCT
TGCCAGCGTTGAGGATATCT

Downstream 100 bases:

>100_bases
CCCCTCCTTTCATTTCACCCGGGCGGACGCAATCTGTACCGCCCACCTCCCTTCTCATTTGCCATCCCCGTGTAAAACAC
GGCGAAATTTGCCATAATGC

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 135; Mature: 135

Protein sequence:

>135_residues
MRVLHTMLRVADLKRSVDFYTQVLGMRLLRTSENEAYKYSLAFVGYGDEEQGAVIELTYNWGVDSYEMGSAFGHIALGVD
DVAATVGQIRRAGGNITREAGPVKGGHTIIAFVEDPDGYKIELIENRSASEGLGR

Sequences:

>Translated_135_residues
MRVLHTMLRVADLKRSVDFYTQVLGMRLLRTSENEAYKYSLAFVGYGDEEQGAVIELTYNWGVDSYEMGSAFGHIALGVD
DVAATVGQIRRAGGNITREAGPVKGGHTIIAFVEDPDGYKIELIENRSASEGLGR
>Mature_135_residues
MRVLHTMLRVADLKRSVDFYTQVLGMRLLRTSENEAYKYSLAFVGYGDEEQGAVIELTYNWGVDSYEMGSAFGHIALGVD
DVAATVGQIRRAGGNITREAGPVKGGHTIIAFVEDPDGYKIELIENRSASEGLGR

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=151, Percent_Identity=35.7615894039735, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1787940, Length=134, Percent_Identity=74.6268656716418, Blast_Score=213, Evalue=4e-57,
Organism=Caenorhabditis elegans, GI17552228, Length=133, Percent_Identity=30.0751879699248, Blast_Score=70, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323639, Length=136, Percent_Identity=39.7058823529412, Blast_Score=100, Evalue=8e-23,
Organism=Drosophila melanogaster, GI20129743, Length=148, Percent_Identity=35.8108108108108, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI19920404, Length=140, Percent_Identity=34.2857142857143, Blast_Score=80, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 14802; Mature: 14802

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLHTMLRVADLKRSVDFYTQVLGMRLLRTSENEAYKYSLAFVGYGDEEQGAVIELTYN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCEEEEEEEC
WGVDSYEMGSAFGHIALGVDDVAATVGQIRRAGGNITREAGPVKGGHTIIAFVEDPDGYK
CCCCCEECCCCCCEEEECHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCE
IELIENRSASEGLGR
EEEEECCCCCCCCCC
>Mature Secondary Structure
MRVLHTMLRVADLKRSVDFYTQVLGMRLLRTSENEAYKYSLAFVGYGDEEQGAVIELTYN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCEEEEEEEC
WGVDSYEMGSAFGHIALGVDDVAATVGQIRRAGGNITREAGPVKGGHTIIAFVEDPDGYK
CCCCCEECCCCCCEEEECHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCE
IELIENRSASEGLGR
EEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]