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The map label for this gene is rnfE

Identifier: 238919987

GI number: 238919987

Start: 2012735

End: 2013445

Strand: Direct

Name: rnfE

Synonym: NT01EI_2091

Alternate gene names: 238919987

Gene position: 2012735-2013445 (Clockwise)

Preceding gene: 238919986

Following gene: 238919988

Centisome position: 52.8

GC content: 62.03

Gene sequence:

>711_bases
ATGAGTGAAGCCAAAAATTTATTTATCAACGGGCTATGGAAGAACAACTCGGCCCTGGTACAGCTGCTCGGCCTCTGTCC
ACTGCTGGCAGTCTCCTCGACCGCCACCAACGCCCTGGGGCTGGGGCTGGCCACCACACTGGTGCTGGTCTTGACCAACA
GCGCCGTTTCGGCGCTGCGCCGCTGGGTACCCGGCGAGATCCGCATCCCTATCTACGTGATGATCATCGCCTCCGTCGTC
AGCGCGGTGCAGATGCTGATCAATGCCTACGCGTTCGGGTTGTATCAATCCCTGGGGATCTTTATTCCGCTGATCGTCAC
CAACTGCATCGTCATCGGGCGCGCCGAGGCCTATGCCGCCCACCATCCCGTCGGGATGGCGGCCCTGGACGGCCTGGCCA
CCGGCTTGGGTGCCACCGCGGCGCTGTTCGTTTTGGGCGCCCTGCGTGAAATCCTGGGCAACGGCACCCTGTTCGACGGC
GCCGACCTGCTGCTCGGCCACTGGGCCCGCGCGCTGCGCATTGAACTGTTTCATACCGATACGCCGTTCCTGCTGGCCAT
TTTGCCGCCGGGCGCATTTCTTGGTCTTGGGTTTATGCTGGCATTCAAATATGTTATCGAACAAAAGCGACGCCAGCGTC
AGGCGCAGCGTAGTGCCGTTGGCCAAGCGCTACGCGGCGCGGCGCCTACGGACAACCATGAGCAGGCATGA

Upstream 100 bases:

>100_bases
TCACCCCGCGGGCAGTGGTTAACGCCGTTCGCCGTACCACGCTGGCGATCCGCGATATCCGACCGCATCTGGCCAGCCTG
CCGCGCTGTGGAGAAAGTAA

Downstream 100 bases:

>100_bases
TGAACCAGGCAAAACGTATTGAAATTCTTCGGCGGCTGCGCGACGCCAACCCACAGCCGACCACCGAGCTGATCTACAGC
ACTCCATTTGAACTGCTGAT

Product: electron transport complex RsxE subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 236; Mature: 235

Protein sequence:

>236_residues
MSEAKNLFINGLWKNNSALVQLLGLCPLLAVSSTATNALGLGLATTLVLVLTNSAVSALRRWVPGEIRIPIYVMIIASVV
SAVQMLINAYAFGLYQSLGIFIPLIVTNCIVIGRAEAYAAHHPVGMAALDGLATGLGATAALFVLGALREILGNGTLFDG
ADLLLGHWARALRIELFHTDTPFLLAILPPGAFLGLGFMLAFKYVIEQKRRQRQAQRSAVGQALRGAAPTDNHEQA

Sequences:

>Translated_236_residues
MSEAKNLFINGLWKNNSALVQLLGLCPLLAVSSTATNALGLGLATTLVLVLTNSAVSALRRWVPGEIRIPIYVMIIASVV
SAVQMLINAYAFGLYQSLGIFIPLIVTNCIVIGRAEAYAAHHPVGMAALDGLATGLGATAALFVLGALREILGNGTLFDG
ADLLLGHWARALRIELFHTDTPFLLAILPPGAFLGLGFMLAFKYVIEQKRRQRQAQRSAVGQALRGAAPTDNHEQA
>Mature_235_residues
SEAKNLFINGLWKNNSALVQLLGLCPLLAVSSTATNALGLGLATTLVLVLTNSAVSALRRWVPGEIRIPIYVMIIASVVS
AVQMLINAYAFGLYQSLGIFIPLIVTNCIVIGRAEAYAAHHPVGMAALDGLATGLGATAALFVLGALREILGNGTLFDGA
DLLLGHWARALRIELFHTDTPFLLAILPPGAFLGLGFMLAFKYVIEQKRRQRQAQRSAVGQALRGAAPTDNHEQA

Specific function: May be part of a membrane complex involved in electron transport

COG id: COG4660

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfE

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nqrDE/rnfAE family

Homologues:

Organism=Escherichia coli, GI1787919, Length=212, Percent_Identity=76.4150943396226, Blast_Score=313, Evalue=6e-87,
Organism=Escherichia coli, GI1787914, Length=156, Percent_Identity=34.6153846153846, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNFE_EDWI9 (C5BE26)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002933502.1
- GeneID:   7959069
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_2091
- OMA:   ENPVTKM
- ProtClustDB:   PRK12405
- GO:   GO:0006810
- HAMAP:   MF_00478
- InterPro:   IPR003667
- InterPro:   IPR010968
- PIRSF:   PIRSF006102
- TIGRFAMs:   TIGR01948

Pfam domain/function: PF02508 Rnf-Nqr

EC number: NA

Molecular weight: Translated: 25084; Mature: 24953

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1d8ee790)-; HASH(0x1dad5f40)-; HASH(0x1daae280)-; HASH(0x1d1937cc)-; HASH(0x1daae298)-; HASH(0x1d089744)-;

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEAKNLFINGLWKNNSALVQLLGLCPLLAVSSTATNALGLGLATTLVLVLTNSAVSALR
CCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RWVPGEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFIPLIVTNCIVIGRAEAYAA
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
HHPVGMAALDGLATGLGATAALFVLGALREILGNGTLFDGADLLLGHWARALRIELFHTD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHEEEEECC
TPFLLAILPPGAFLGLGFMLAFKYVIEQKRRQRQAQRSAVGQALRGAAPTDNHEQA
CCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
>Mature Secondary Structure 
SEAKNLFINGLWKNNSALVQLLGLCPLLAVSSTATNALGLGLATTLVLVLTNSAVSALR
CCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RWVPGEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFIPLIVTNCIVIGRAEAYAA
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
HHPVGMAALDGLATGLGATAALFVLGALREILGNGTLFDGADLLLGHWARALRIELFHTD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHEEEEECC
TPFLLAILPPGAFLGLGFMLAFKYVIEQKRRQRQAQRSAVGQALRGAAPTDNHEQA
CCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA