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The map label for this gene is nth [H]

Identifier: 238919988

GI number: 238919988

Start: 2013442

End: 2014086

Strand: Direct

Name: nth [H]

Synonym: NT01EI_2092

Alternate gene names: 238919988

Gene position: 2013442-2014086 (Clockwise)

Preceding gene: 238919987

Following gene: 238919989

Centisome position: 52.81

GC content: 60.0

Gene sequence:

>645_bases
ATGATGAACCAGGCAAAACGTATTGAAATTCTTCGGCGGCTGCGCGACGCCAACCCACAGCCGACCACCGAGCTGATCTA
CAGCACTCCATTTGAACTGCTGATCGCCGTACTGCTGTCGGCCCAGGCGACCGACGTCAGCGTCAATAAGGCTACCGCTA
CGCTATTTCCGGCGGCCAACACTCCCACAGCGCTGCTGGCGCTCGGCGTGGACGGCGTCAAGCAGCACATCAAGACCATT
GGTCTGTACAACGGTAAAGCGGAAAACATCATCAAGACCTGCCGCCTCCTGCTGGAGCAGCACGGCGGTGAAGTGCCAGA
AAATCGCCAGGCGCTGGAGGCGCTGCCCGGCGTCGGGCGCAAGACCGCCAACGTCGTGCTCAATACGGCCTTCGGCTGGC
CAACCATCGCTGTCGATACCCATATTTTCCGCGTCTGTAACCGTACCCGCTTTGCCCCGGGAAAGACGGTCAACGAGGTT
GAGGAGAAGCTGCTTAAGGTAGTACCGGCAGAGTTCGCCCTCAACTGCCATCACTGGCTGATCCTGCACGGGCGCTATAC
CTGCATCGCCCGGCGCCCGCGCTGCGGCTCCTGTCTCATCGAGGATCTGTGCGAATTCCGCGATAAAGTCTACCCCGAGC
AGTAA

Upstream 100 bases:

>100_bases
AATATGTTATCGAACAAAAGCGACGCCAGCGTCAGGCGCAGCGTAGTGCCGTTGGCCAAGCGCTACGCGGCGCGGCGCCT
ACGGACAACCATGAGCAGGC

Downstream 100 bases:

>100_bases
GCCCTTTCCCGGCGCCGCGCGCTGCTTGTCCGGCAGTGCGCGCCGCCCTCTGCTGTGATCTCTACCCCATTTTTACCCCT
GCTAGCGCAGTTTCACTTTA

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MMNQAKRIEILRRLRDANPQPTTELIYSTPFELLIAVLLSAQATDVSVNKATATLFPAANTPTALLALGVDGVKQHIKTI
GLYNGKAENIIKTCRLLLEQHGGEVPENRQALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTRFAPGKTVNEV
EEKLLKVVPAEFALNCHHWLILHGRYTCIARRPRCGSCLIEDLCEFRDKVYPEQ

Sequences:

>Translated_214_residues
MMNQAKRIEILRRLRDANPQPTTELIYSTPFELLIAVLLSAQATDVSVNKATATLFPAANTPTALLALGVDGVKQHIKTI
GLYNGKAENIIKTCRLLLEQHGGEVPENRQALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTRFAPGKTVNEV
EEKLLKVVPAEFALNCHHWLILHGRYTCIARRPRCGSCLIEDLCEFRDKVYPEQ
>Mature_214_residues
MMNQAKRIEILRRLRDANPQPTTELIYSTPFELLIAVLLSAQATDVSVNKATATLFPAANTPTALLALGVDGVKQHIKTI
GLYNGKAENIIKTCRLLLEQHGGEVPENRQALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTRFAPGKTVNEV
EEKLLKVVPAEFALNCHHWLILHGRYTCIARRPRCGSCLIEDLCEFRDKVYPEQ

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA dam

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=31.0734463276836, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI1787920, Length=209, Percent_Identity=83.2535885167464, Blast_Score=370, Evalue=1e-104,
Organism=Escherichia coli, GI1789331, Length=186, Percent_Identity=27.9569892473118, Blast_Score=65, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17554540, Length=175, Percent_Identity=32, Blast_Score=94, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6324530, Length=192, Percent_Identity=26.5625, Blast_Score=73, Evalue=4e-14,
Organism=Drosophila melanogaster, GI45550361, Length=178, Percent_Identity=30.8988764044944, Blast_Score=95, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 23823; Mature: 23823

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNQAKRIEILRRLRDANPQPTTELIYSTPFELLIAVLLSAQATDVSVNKATATLFPAAN
CCCHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHEEEECCCC
TPTALLALGVDGVKQHIKTIGLYNGKAENIIKTCRLLLEQHGGEVPENRQALEALPGVGR
CCHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCC
KTANVVLNTAFGWPTIAVDTHIFRVCNRTRFAPGKTVNEVEEKLLKVVPAEFALNCHHWL
CHHHHEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEE
ILHGRYTCIARRPRCGSCLIEDLCEFRDKVYPEQ
EEECCEEEEECCCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MMNQAKRIEILRRLRDANPQPTTELIYSTPFELLIAVLLSAQATDVSVNKATATLFPAAN
CCCHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHEEEECCCC
TPTALLALGVDGVKQHIKTIGLYNGKAENIIKTCRLLLEQHGGEVPENRQALEALPGVGR
CCHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCC
KTANVVLNTAFGWPTIAVDTHIFRVCNRTRFAPGKTVNEVEEKLLKVVPAEFALNCHHWL
CHHHHEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEE
ILHGRYTCIARRPRCGSCLIEDLCEFRDKVYPEQ
EEECCEEEEECCCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]