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The map label for this gene is mlc [H]

Identifier: 238919967

GI number: 238919967

Start: 1991256

End: 1992479

Strand: Reverse

Name: mlc [H]

Synonym: NT01EI_2071

Alternate gene names: 238919967

Gene position: 1992479-1991256 (Counterclockwise)

Preceding gene: 238919969

Following gene: 238919966

Centisome position: 52.26

GC content: 57.27

Gene sequence:

>1224_bases
GTGATTGCTGACGGTCAGCCGGGGCATATTGATCAAATCAAGCAGATAAATGCTGGGGCGGTTTACCGCCTTATCGATCG
TTTTGGCCCTATTTCGCGCATTGAACTTTCAAAACGTGCACAGCTGGCTCCCGCCAGTATCACCAAAATCGTTCGTGAAC
TGCTTGAAGCTCACCTGGTGCAGGAGACGGAGTTTCAGGAGCCCGGCAGCCGCGGACGTCCTGCCGTAGGTCTCACGCTG
GATACCGCATCGTGGCACTATCTGTGCGTGCGGATTGGGCTGGGCTCCATGACGTTGGCGCTGCGCGATCTGCGCGGTAC
GCTGGTCGCCGAAGAGGAGATCGCACTGTCGGTGCGGGGTGAGCTGCCGCTGCTGGCCCGCATCGTAACAGAGATCGATC
TGTTTTTTACTCGCCACCAGCATCAGCTGGAACGCCTGACGGCGATAGCCATTACGCTGCCGGGATTAATTAATGCCGCC
CGGGGGATCGTTCACCGGATGCCGTATTATACCGAGGTGCGTGATATGGCGCTAAGCGCCGAGCTCTCACGCCACACTGG
GCTGCCGGTATATCTGCAGCATGATGTTTGCGCCTGGACCATAGCCGAGTCGCTGTTTGGCGCCGGTGTGGGCTGTGCCA
ACCTGATTCAGATTGTCATTGACCATAACGTGGGGGCGGGGGTGATCAGCGAAGGGCGCCTGCTGAACGGGAGCCGCCAA
AGTCTGGTTGAAATAGGGCATACCCAGGTCGATCCCTATGGCAAACAGTGCTACTGCGGAAACCATGGCTGTCTGGAGAC
GATTGCCGGACTGGACAATATACTGACGCTGGCGCAGCAGCGCCTCGCGCTGTCGCCGTCGTCACAGCTGCACACGGTCA
ACCCGCTGAATATTGAGGCCTTGTGTCAGGCCGCACTGCAGGGAGATCAGCTGGGGCGAGACTTGATCAATAGCGTTGGC
CATAGTGTGGGGCGTATCGCCGCGGTGATGGTTAACCTGTTTAATCCGGAAAAAATTCTGATAGGTTCACCGCTGAACCA
GGCCGCGGAGCTCTTGTTCCCCTCCATTGCCAGCGCAATTCGACAGCAGTCGCTTCCTCATTACACCCAGTATCTCTCGG
TTGAAGCCAGCCATTTCCACAATCATGGTACAATGCCTGCCGCCGCGTTGGTCAAAGAGGCGCTGTATAACGGTTCGCTG
TTAGTACAGTTACTGCAAGGGTAA

Upstream 100 bases:

>100_bases
ATCACATAACTAATCAAATGGTTGATGCCTTTTTGTAAAATACCCACCATACCCGGTAATATTTATTTTGAAGCGCGAAA
AATAACTGGGAGTAGCAGTG

Downstream 100 bases:

>100_bases
CTGTTCGCTTGTATGTGCCGTATTGTTACTTATATCCCGTGCATTTGCCCTACGGGCAGGAAAATGATCCGTGCGATAAA
TAAAAATTTGAGCTAACGCA

Product: protein mlc

Products: NA

Alternate protein names: Making large colonies protein [H]

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MIADGQPGHIDQIKQINAGAVYRLIDRFGPISRIELSKRAQLAPASITKIVRELLEAHLVQETEFQEPGSRGRPAVGLTL
DTASWHYLCVRIGLGSMTLALRDLRGTLVAEEEIALSVRGELPLLARIVTEIDLFFTRHQHQLERLTAIAITLPGLINAA
RGIVHRMPYYTEVRDMALSAELSRHTGLPVYLQHDVCAWTIAESLFGAGVGCANLIQIVIDHNVGAGVISEGRLLNGSRQ
SLVEIGHTQVDPYGKQCYCGNHGCLETIAGLDNILTLAQQRLALSPSSQLHTVNPLNIEALCQAALQGDQLGRDLINSVG
HSVGRIAAVMVNLFNPEKILIGSPLNQAAELLFPSIASAIRQQSLPHYTQYLSVEASHFHNHGTMPAAALVKEALYNGSL
LVQLLQG

Sequences:

>Translated_407_residues
MIADGQPGHIDQIKQINAGAVYRLIDRFGPISRIELSKRAQLAPASITKIVRELLEAHLVQETEFQEPGSRGRPAVGLTL
DTASWHYLCVRIGLGSMTLALRDLRGTLVAEEEIALSVRGELPLLARIVTEIDLFFTRHQHQLERLTAIAITLPGLINAA
RGIVHRMPYYTEVRDMALSAELSRHTGLPVYLQHDVCAWTIAESLFGAGVGCANLIQIVIDHNVGAGVISEGRLLNGSRQ
SLVEIGHTQVDPYGKQCYCGNHGCLETIAGLDNILTLAQQRLALSPSSQLHTVNPLNIEALCQAALQGDQLGRDLINSVG
HSVGRIAAVMVNLFNPEKILIGSPLNQAAELLFPSIASAIRQQSLPHYTQYLSVEASHFHNHGTMPAAALVKEALYNGSL
LVQLLQG
>Mature_407_residues
MIADGQPGHIDQIKQINAGAVYRLIDRFGPISRIELSKRAQLAPASITKIVRELLEAHLVQETEFQEPGSRGRPAVGLTL
DTASWHYLCVRIGLGSMTLALRDLRGTLVAEEEIALSVRGELPLLARIVTEIDLFFTRHQHQLERLTAIAITLPGLINAA
RGIVHRMPYYTEVRDMALSAELSRHTGLPVYLQHDVCAWTIAESLFGAGVGCANLIQIVIDHNVGAGVISEGRLLNGSRQ
SLVEIGHTQVDPYGKQCYCGNHGCLETIAGLDNILTLAQQRLALSPSSQLHTVNPLNIEALCQAALQGDQLGRDLINSVG
HSVGRIAAVMVNLFNPEKILIGSPLNQAAELLFPSIASAIRQQSLPHYTQYLSVEASHFHNHGTMPAAALVKEALYNGSL
LVQLLQG

Specific function: Transcriptional repressor that regulates the expression of proteins that are part of the phosphotransferase system for sugar uptake. Regulates the expression of malT [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Homo sapiens, GI298566325, Length=235, Percent_Identity=28.0851063829787, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI298566315, Length=235, Percent_Identity=28.0851063829787, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI190014632, Length=235, Percent_Identity=28.0851063829787, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI4885285, Length=235, Percent_Identity=28.0851063829787, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1787878, Length=407, Percent_Identity=65.8476658476659, Blast_Score=567, Evalue=1e-163,
Organism=Escherichia coli, GI1786891, Length=404, Percent_Identity=38.1188118811881, Blast_Score=279, Evalue=3e-76,
Organism=Escherichia coli, GI87082127, Length=339, Percent_Identity=21.8289085545723, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: NA

Molecular weight: Translated: 44191; Mature: 44191

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIADGQPGHIDQIKQINAGAVYRLIDRFGPISRIELSKRAQLAPASITKIVRELLEAHLV
CCCCCCCCCHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
QETEFQEPGSRGRPAVGLTLDTASWHYLCVRIGLGSMTLALRDLRGTLVAEEEIALSVRG
HHHCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCHHHHHHHHHHHHEEEHHHEEEEECC
ELPLLARIVTEIDLFFTRHQHQLERLTAIAITLPGLINAARGIVHRMPYYTEVRDMALSA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
ELSRHTGLPVYLQHDVCAWTIAESLFGAGVGCANLIQIVIDHNVGAGVISEGRLLNGSRQ
HHHHCCCCCEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHH
SLVEIGHTQVDPYGKQCYCGNHGCLETIAGLDNILTLAQQRLALSPSSQLHTVNPLNIEA
HHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHH
LCQAALQGDQLGRDLINSVGHSVGRIAAVMVNLFNPEKILIGSPLNQAAELLFPSIASAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHH
RQQSLPHYTQYLSVEASHFHNHGTMPAAALVKEALYNGSLLVQLLQG
HHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure
MIADGQPGHIDQIKQINAGAVYRLIDRFGPISRIELSKRAQLAPASITKIVRELLEAHLV
CCCCCCCCCHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
QETEFQEPGSRGRPAVGLTLDTASWHYLCVRIGLGSMTLALRDLRGTLVAEEEIALSVRG
HHHCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCHHHHHHHHHHHHEEEHHHEEEEECC
ELPLLARIVTEIDLFFTRHQHQLERLTAIAITLPGLINAARGIVHRMPYYTEVRDMALSA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
ELSRHTGLPVYLQHDVCAWTIAESLFGAGVGCANLIQIVIDHNVGAGVISEGRLLNGSRQ
HHHHCCCCCEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHH
SLVEIGHTQVDPYGKQCYCGNHGCLETIAGLDNILTLAQQRLALSPSSQLHTVNPLNIEA
HHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHH
LCQAALQGDQLGRDLINSVGHSVGRIAAVMVNLFNPEKILIGSPLNQAAELLFPSIASAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHH
RQQSLPHYTQYLSVEASHFHNHGTMPAAALVKEALYNGSLLVQLLQG
HHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7766024; 9097039; 9278503 [H]