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The map label for this gene is ynfL [H]

Identifier: 238919969

GI number: 238919969

Start: 1992595

End: 1993587

Strand: Reverse

Name: ynfL [H]

Synonym: NT01EI_2073

Alternate gene names: 238919969

Gene position: 1993587-1992595 (Counterclockwise)

Preceding gene: 238919971

Following gene: 238919967

Centisome position: 52.29

GC content: 55.59

Gene sequence:

>993_bases
ATGTCTAATATATTAATAAGAACCAATAATATATTTAAAGTATCAATGGTCATGCGCATCGAAATTCGTCATCTTAAGTA
CTTTATCGCCGTTGCCGAGGAGCGACATTTTAACCGGGCGGCGGAACGGCTGAATATTTCACAGCCGCCACTGAGCCAGC
AGATCCAGTCGCTGGAGCGTGAACTTGGCGCCTGTCTGCTGATGCGCACCAACCGTAGCGTCAGCCTGACGCAGGCGGGG
AGCCTTTTTCTGAAAGAGTGCTATTCCATCGTTGGCCAACTGGAGCAGGCGGCCGAAAAGGCGGCCCGGGCACATCGGGG
GGAGACCGGGGATCTGACCATTGGCTTTACCTCATCGGTTCCCTTTATTCCCCGTGTGGCGCACAGCCTATTCTCCTATC
GCCATCGCTATCCGCAGGTGCATCTGCAAATGCGTGAACTCAATACCAAGCAGCAGATCGAACCGTTGGTGGATGGAAAG
CTGGATATAGGCATATTGCGTAATACCCTGCTACCTGGCGTGCTGAAGCACCGGGTGCTGCAGCGTGAGCCGCTGGTTGC
GGTTTTGCACCGTCATCATCCCTTGGCGCAGCGGGGACAGGCGCTGAGATTTAGCGACCTGGCCGACCAACCCTTTGTCT
TCTTTTCCCGCGGCGATGGTACGGCGCTGTACGATGAGCTGCTGTCGCTGCTGAAGAGGGCAGGCATTGTGCCGTATATT
ACGCAGGAGGTCGGTGAAGCGATGACGATCGTCGGGCTGATCTCAGCGGGCCTGGGGGTCTCTATCCTGCCGGCATCCTA
TACCCGTATTCAGGTGGACGGCGTTTGTTATCGCGCATTGGATGAGCCTGATGCCTATACCGAGGTGTGGCTTGCCTACC
ATCGCCAGCGGCCGCTGAGTGCACAGGCTACTGCGCTGATCGCGCTGTTGACTGCGCCATCGTCTAATCTTATTGCGTCA
CTGGCCATGAGTGAAAACACACCGAACAGATAA

Upstream 100 bases:

>100_bases
GTATGGAGCAAACAGAGAGTCAGCACTGGGGGCATCACAAGCTGCGATGTTGACACGGAGCCAGGCTATGTGATGGCGAG
CATAGAATTGCGCCTGTTGA

Downstream 100 bases:

>100_bases
GTATTTGTGCACTTAATCACATAACTAATCAAATGGTTGATGCCTTTTTGTAAAATACCCACCATACCCGGTAATATTTA
TTTTGAAGCGCGAAAAATAA

Product: HTH-type transcriptional regulator AlsR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MSNILIRTNNIFKVSMVMRIEIRHLKYFIAVAEERHFNRAAERLNISQPPLSQQIQSLERELGACLLMRTNRSVSLTQAG
SLFLKECYSIVGQLEQAAEKAARAHRGETGDLTIGFTSSVPFIPRVAHSLFSYRHRYPQVHLQMRELNTKQQIEPLVDGK
LDIGILRNTLLPGVLKHRVLQREPLVAVLHRHHPLAQRGQALRFSDLADQPFVFFSRGDGTALYDELLSLLKRAGIVPYI
TQEVGEAMTIVGLISAGLGVSILPASYTRIQVDGVCYRALDEPDAYTEVWLAYHRQRPLSAQATALIALLTAPSSNLIAS
LAMSENTPNR

Sequences:

>Translated_330_residues
MSNILIRTNNIFKVSMVMRIEIRHLKYFIAVAEERHFNRAAERLNISQPPLSQQIQSLERELGACLLMRTNRSVSLTQAG
SLFLKECYSIVGQLEQAAEKAARAHRGETGDLTIGFTSSVPFIPRVAHSLFSYRHRYPQVHLQMRELNTKQQIEPLVDGK
LDIGILRNTLLPGVLKHRVLQREPLVAVLHRHHPLAQRGQALRFSDLADQPFVFFSRGDGTALYDELLSLLKRAGIVPYI
TQEVGEAMTIVGLISAGLGVSILPASYTRIQVDGVCYRALDEPDAYTEVWLAYHRQRPLSAQATALIALLTAPSSNLIAS
LAMSENTPNR
>Mature_329_residues
SNILIRTNNIFKVSMVMRIEIRHLKYFIAVAEERHFNRAAERLNISQPPLSQQIQSLERELGACLLMRTNRSVSLTQAGS
LFLKECYSIVGQLEQAAEKAARAHRGETGDLTIGFTSSVPFIPRVAHSLFSYRHRYPQVHLQMRELNTKQQIEPLVDGKL
DIGILRNTLLPGVLKHRVLQREPLVAVLHRHHPLAQRGQALRFSDLADQPFVFFSRGDGTALYDELLSLLKRAGIVPYIT
QEVGEAMTIVGLISAGLGVSILPASYTRIQVDGVCYRALDEPDAYTEVWLAYHRQRPLSAQATALIALLTAPSSNLIASL
AMSENTPNR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=284, Percent_Identity=55.9859154929578, Blast_Score=320, Evalue=7e-89,
Organism=Escherichia coli, GI1788887, Length=263, Percent_Identity=34.9809885931559, Blast_Score=134, Evalue=7e-33,
Organism=Escherichia coli, GI2367136, Length=302, Percent_Identity=31.1258278145695, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1790399, Length=286, Percent_Identity=30.7692307692308, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI145693105, Length=250, Percent_Identity=28.4, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788748, Length=254, Percent_Identity=29.5275590551181, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI157672245, Length=265, Percent_Identity=32.0754716981132, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1788297, Length=254, Percent_Identity=28.740157480315, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1790208, Length=251, Percent_Identity=27.8884462151394, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI87081904, Length=218, Percent_Identity=28.8990825688073, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1787601, Length=165, Percent_Identity=32.1212121212121, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788481, Length=288, Percent_Identity=25.6944444444444, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1786401, Length=167, Percent_Identity=31.1377245508982, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1787530, Length=257, Percent_Identity=25.6809338521401, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1789506, Length=258, Percent_Identity=24.4186046511628, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1786713, Length=262, Percent_Identity=27.0992366412214, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1787806, Length=304, Percent_Identity=22.3684210526316, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1789173, Length=145, Percent_Identity=32.4137931034483, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1787589, Length=150, Percent_Identity=29.3333333333333, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1789204, Length=315, Percent_Identity=26.3492063492064, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI1789639, Length=251, Percent_Identity=25.4980079681275, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36926; Mature: 36795

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNILIRTNNIFKVSMVMRIEIRHLKYFIAVAEERHFNRAAERLNISQPPLSQQIQSLER
CCCEEEEECCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
ELGACLLMRTNRSVSLTQAGSLFLKECYSIVGQLEQAAEKAARAHRGETGDLTIGFTSSV
HHHHEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCC
PFIPRVAHSLFSYRHRYPQVHLQMRELNTKQQIEPLVDGKLDIGILRNTLLPGVLKHRVL
CHHHHHHHHHHHHHHCCCHHEEHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
QREPLVAVLHRHHPLAQRGQALRFSDLADQPFVFFSRGDGTALYDELLSLLKRAGIVPYI
HCCHHHHHHHCCCCHHHHCCCEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCHHH
TQEVGEAMTIVGLISAGLGVSILPASYTRIQVDGVCYRALDEPDAYTEVWLAYHRQRPLS
HHHHHHHHHHHHHHHCCCCCEEECCCCEEEEECCEEEECCCCCCHHHHHHHHHHHCCCCC
AQATALIALLTAPSSNLIASLAMSENTPNR
HHHHHHHHHEECCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SNILIRTNNIFKVSMVMRIEIRHLKYFIAVAEERHFNRAAERLNISQPPLSQQIQSLER
CCEEEEECCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
ELGACLLMRTNRSVSLTQAGSLFLKECYSIVGQLEQAAEKAARAHRGETGDLTIGFTSSV
HHHHEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCC
PFIPRVAHSLFSYRHRYPQVHLQMRELNTKQQIEPLVDGKLDIGILRNTLLPGVLKHRVL
CHHHHHHHHHHHHHHCCCHHEEHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
QREPLVAVLHRHHPLAQRGQALRFSDLADQPFVFFSRGDGTALYDELLSLLKRAGIVPYI
HCCHHHHHHHCCCCHHHHCCCEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCHHH
TQEVGEAMTIVGLISAGLGVSILPASYTRIQVDGVCYRALDEPDAYTEVWLAYHRQRPLS
HHHHHHHHHHHHHHHCCCCCEEECCCCEEEEECCEEEECCCCCCHHHHHHHHHHHCCCCC
AQATALIALLTAPSSNLIASLAMSENTPNR
HHHHHHHHHEECCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]