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The map label for this gene is dnaB [H]

Identifier: 238919926

GI number: 238919926

Start: 1954644

End: 1956026

Strand: Reverse

Name: dnaB [H]

Synonym: NT01EI_2030

Alternate gene names: 238919926

Gene position: 1956026-1954644 (Counterclockwise)

Preceding gene: 238919927

Following gene: 238919925

Centisome position: 51.31

GC content: 65.37

Gene sequence:

>1383_bases
GTGAACGCCAATCTCCAGTACCTGGAGTCCAGTGTGATCGGTGGCCTGCTGCTGGGTGGCCTGACACCGGCTGCGCAGGA
GGTGCTGGCGTCGCTGGAGCCTGCCGCGTTTTCGATCCCGCTGTACCGGACGGTGTACCGGGTTATTCAGCGTCAGGCCA
GAGCCCGTAACCTGATCGACTCGCTGATGGTGGCCGAGGAGTGTGGGGATGAGCACTTTGCCGATGTGATGAGCACGGCC
AAACACTGCCCCAGCGCCGCCAATCTGGCGGGCTATGCGGAAATGGTCAGCCAGGAGTACCAGCGCAGGCAGTTCGCCGT
CACCCTGGATGGCATGCGCCGGGAAATCATCAGCGCCAGTATCGAGCAGGCCGGGCAGGCGATGGATACCCTGATGAGCC
GCCTGTCGCTGATCCGCCGTCCCCGGCTGGAGCCTGTACCGGTGGTGCTGGGCGAGGTGATGGGGGATTTTACCGAGACG
CTGGAGCGGCGCCTGAACAATGGGCTGGAGTCGGACACCCTCCGGCTGGGGATCGCGTCGCTGGATGCGGTGACCGGCGG
GGTGAATCCGCAGGATTTGATCATCATCGCCGGGCGCCCGGGGATGGGGAAAACCGCGCTGGCCATGCGCATTGCCACGT
CGGTGGCCGCCCGCACGTTACCCGGCACCTCACAGCGGCGCGGGGTGCTGATCTTCAGCCTGGAGATGGGCGCGCAGCAG
TTGGCCGAGCGGGGCATTGCCGCAGCCGGCGGGCTGGCGGTGTCGGTGCTGCGTAATCCGGCCACGCTGGATGACGAGGG
CTGGGGGCGGGTATCGCAGGGCGTCGCGGCGCTGGATGGGCTGGATCTCTGGATCGTCGATACCGCCCGTTTGAGCGTGG
AGAAAATCCGCGCCATGGCCGAGCGCCAAAAGCAGGCACACCCGACGCTGTCGCTGATCCTGGTGGACTATCTGGGGCTG
ATCGAAAAACCGCGCGCCGAGCGCCACGATCTGGCCATCGCGCATATTTCCGGCAGCCTGAAGAGCATGGCGAAGGAGCT
GGGGACGCCGGTGATTGCGCTGAGCCAGCTGTCCCGCGAGGTGGAAAAACGCCCCAACAAGCGGCCGGTCAGCGCCGATC
TGCGTGACTCCGGCAGCATTGAGCAGGATGCCGACCTGATCGTCATGCTGTACCGCGACGTGATTTATCACCCCGATACC
CCGGCCCGGCACCATGCGGAGCTGATCGTCACCAAAAGCCGCTTTGGTCAGGCCGGGGCGGTGATTTACCAGCGTTTTAT
CAACGGCCATTTTGTGGAGTGCGATCAGGAGGAGGCGCGGCGGCTGTGTACTCCGATGGATCAGCCGCCGCTGGCAGCGC
GTTACCGCGGCGCCAGAGTGTGA

Upstream 100 bases:

>100_bases
CCAAGCCGTTTTACTTTGGCTTCGGCGATGCGGGCTGGATCGCCGATTTTGATTACCTGCTGCGCCAGAAAACCCTGACC
CGGGTACGGGAGGGGACACT

Downstream 100 bases:

>100_bases
GCGCCGACATGAGACAGGGAGGAGAGACGATGGACGTGAAACCGAGCGTCAGTTTTCAAGAGCGTGCCAGCATCAACAAC
GGGCTGCGGGCGCTGAGCCG

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MNANLQYLESSVIGGLLLGGLTPAAQEVLASLEPAAFSIPLYRTVYRVIQRQARARNLIDSLMVAEECGDEHFADVMSTA
KHCPSAANLAGYAEMVSQEYQRRQFAVTLDGMRREIISASIEQAGQAMDTLMSRLSLIRRPRLEPVPVVLGEVMGDFTET
LERRLNNGLESDTLRLGIASLDAVTGGVNPQDLIIIAGRPGMGKTALAMRIATSVAARTLPGTSQRRGVLIFSLEMGAQQ
LAERGIAAAGGLAVSVLRNPATLDDEGWGRVSQGVAALDGLDLWIVDTARLSVEKIRAMAERQKQAHPTLSLILVDYLGL
IEKPRAERHDLAIAHISGSLKSMAKELGTPVIALSQLSREVEKRPNKRPVSADLRDSGSIEQDADLIVMLYRDVIYHPDT
PARHHAELIVTKSRFGQAGAVIYQRFINGHFVECDQEEARRLCTPMDQPPLAARYRGARV

Sequences:

>Translated_460_residues
MNANLQYLESSVIGGLLLGGLTPAAQEVLASLEPAAFSIPLYRTVYRVIQRQARARNLIDSLMVAEECGDEHFADVMSTA
KHCPSAANLAGYAEMVSQEYQRRQFAVTLDGMRREIISASIEQAGQAMDTLMSRLSLIRRPRLEPVPVVLGEVMGDFTET
LERRLNNGLESDTLRLGIASLDAVTGGVNPQDLIIIAGRPGMGKTALAMRIATSVAARTLPGTSQRRGVLIFSLEMGAQQ
LAERGIAAAGGLAVSVLRNPATLDDEGWGRVSQGVAALDGLDLWIVDTARLSVEKIRAMAERQKQAHPTLSLILVDYLGL
IEKPRAERHDLAIAHISGSLKSMAKELGTPVIALSQLSREVEKRPNKRPVSADLRDSGSIEQDADLIVMLYRDVIYHPDT
PARHHAELIVTKSRFGQAGAVIYQRFINGHFVECDQEEARRLCTPMDQPPLAARYRGARV
>Mature_460_residues
MNANLQYLESSVIGGLLLGGLTPAAQEVLASLEPAAFSIPLYRTVYRVIQRQARARNLIDSLMVAEECGDEHFADVMSTA
KHCPSAANLAGYAEMVSQEYQRRQFAVTLDGMRREIISASIEQAGQAMDTLMSRLSLIRRPRLEPVPVVLGEVMGDFTET
LERRLNNGLESDTLRLGIASLDAVTGGVNPQDLIIIAGRPGMGKTALAMRIATSVAARTLPGTSQRRGVLIFSLEMGAQQ
LAERGIAAAGGLAVSVLRNPATLDDEGWGRVSQGVAALDGLDLWIVDTARLSVEKIRAMAERQKQAHPTLSLILVDYLGL
IEKPRAERHDLAIAHISGSLKSMAKELGTPVIALSQLSREVEKRPNKRPVSADLRDSGSIEQDADLIVMLYRDVIYHPDT
PARHHAELIVTKSRFGQAGAVIYQRFINGHFVECDQEEARRLCTPMDQPPLAARYRGARV

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=446, Percent_Identity=34.0807174887892, Blast_Score=212, Evalue=5e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 50170; Mature: 50170

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNANLQYLESSVIGGLLLGGLTPAAQEVLASLEPAAFSIPLYRTVYRVIQRQARARNLID
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SLMVAEECGDEHFADVMSTAKHCPSAANLAGYAEMVSQEYQRRQFAVTLDGMRREIISAS
HHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHH
IEQAGQAMDTLMSRLSLIRRPRLEPVPVVLGEVMGDFTETLERRLNNGLESDTLRLGIAS
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
LDAVTGGVNPQDLIIIAGRPGMGKTALAMRIATSVAARTLPGTSQRRGVLIFSLEMGAQQ
HHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHH
LAERGIAAAGGLAVSVLRNPATLDDEGWGRVSQGVAALDGLDLWIVDTARLSVEKIRAMA
HHHCCCCHHCCHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHH
ERQKQAHPTLSLILVDYLGLIEKPRAERHDLAIAHISGSLKSMAKELGTPVIALSQLSRE
HHHHHHCCHHHHHHHHHHHHHCCCCCHHHCEEEEHHHHHHHHHHHHHCCCHHHHHHHHHH
VEKRPNKRPVSADLRDSGSIEQDADLIVMLYRDVIYHPDTPARHHAELIVTKSRFGQAGA
HHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHEEEEHHHCCCHHH
VIYQRFINGHFVECDQEEARRLCTPMDQPPLAARYRGARV
HHHHHHHCCCEEECCHHHHHHHCCCCCCCCCHHHCCCCCC
>Mature Secondary Structure
MNANLQYLESSVIGGLLLGGLTPAAQEVLASLEPAAFSIPLYRTVYRVIQRQARARNLID
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SLMVAEECGDEHFADVMSTAKHCPSAANLAGYAEMVSQEYQRRQFAVTLDGMRREIISAS
HHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHH
IEQAGQAMDTLMSRLSLIRRPRLEPVPVVLGEVMGDFTETLERRLNNGLESDTLRLGIAS
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
LDAVTGGVNPQDLIIIAGRPGMGKTALAMRIATSVAARTLPGTSQRRGVLIFSLEMGAQQ
HHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHH
LAERGIAAAGGLAVSVLRNPATLDDEGWGRVSQGVAALDGLDLWIVDTARLSVEKIRAMA
HHHCCCCHHCCHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHH
ERQKQAHPTLSLILVDYLGLIEKPRAERHDLAIAHISGSLKSMAKELGTPVIALSQLSRE
HHHHHHCCHHHHHHHHHHHHHCCCCCHHHCEEEEHHHHHHHHHHHHHCCCHHHHHHHHHH
VEKRPNKRPVSADLRDSGSIEQDADLIVMLYRDVIYHPDTPARHHAELIVTKSRFGQAGA
HHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHEEEEHHHCCCHHH
VIYQRFINGHFVECDQEEARRLCTPMDQPPLAARYRGARV
HHHHHHHCCCEEECCHHHHHHHCCCCCCCCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]