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The map label for this gene is sdaA [H]

Identifier: 238919753

GI number: 238919753

Start: 1793435

End: 1794802

Strand: Reverse

Name: sdaA [H]

Synonym: NT01EI_1857

Alternate gene names: 238919753

Gene position: 1794802-1793435 (Counterclockwise)

Preceding gene: 238919754

Following gene: 238919744

Centisome position: 47.08

GC content: 61.11

Gene sequence:

>1368_bases
GTGATTAGCGTTTTCGACATGTTCAAGATCGGCATTGGCCCCTCCAGTTCCCATACGGTCGGACCGATGAAAGCCGGGAA
ACAGTTTGTCGATGATCTGGCGGCACAGGGTCTGTTGCCGTCGGTCACGCGCGTTGCCGTTGACGTTTACGGCTCGCTCT
CACTGACCGGTAAAGGCCATCACACCGATATCGCCATCATTATGGGGCTGGCGGGAAATCTTCCTGACAGCGTCGACATC
GACGCCATTCCCGAGTTTATCCACAGCGTGGAGAGCAGCGAACGGCTGCCCCTGGGGCGCGAAGGCCACCCGGTCATTTT
CCCACGCGACGGCGGAATGGTCTTTCGCAGCGATAACCTGCCGCTGCATGAAAACGGCATGACCATCCGTGCCTACGGCG
CCGGTGACGCCCCGCTGTACAGCAAAACCTACTACTCCATCGGCGGCGGCTTTATCGTTGATGAAGAGCACTTTGGCCAG
CAAAACGACGACGGCGTCAAAGTCCCCTATCCGTTCCACTCCGCGCAGGCGCTGCTGGACTACTGTGAAGAGACCGGTCT
TTCCATCAGCGGGCTGGTGATGAAAAATGAGCTGGCGCTGCACAGCAAAGAAGAGATTTACGCCTACTTTACCGCCATCT
GGGACACCATGCAGGCCTGCATTGAGCGCGGGCTGAACACCGAAGGCATACTGCCCGGGCCGCTGCGGGTGCCGCGACGC
GCCGCCGCACTGCGCCGGATGCTGGTCACCAGCGACAAATACTCGCGCGATCCGATGAATGTGATCGACTGGGTCAATAT
GTTTGCCCTGGCGGTCAATGAAGAAAACGCCGCCGGTGGACGCGTCGTTACCGCACCGACCAACGGTGCCTGCGGCATCG
TGCCGGCCGTGCTGGCCTATTACGATCACTTTATCGATCCCGTAACGCCGGACGTCTTCGTGCGCTACTTCCTGGCCAGC
GGTGCTATCGGCGCCCTGTACAAGATGAACGCCTCGATCTCCGGCGCCGAGGTCGGCTGTCAGGGCGAGGTGGGCGTCGC
TTGCTCCATGGCCGCCGCCGGGCTGGCTGAGCTGCTGGGCGGCAGCCCGGTACAGGTGTGCATCGCTGCGGAGATCGGTA
TGGAGCACAATCTGGGGCTGACCTGCGATCCGGTCGCCGGTCAGGTACAGGTGCCGTGCATCGAGCGTAACGCCATTGCC
TCGGTAAAAGCGATCAACGCGGCCCGTATGGCGCTGCAGCGCACCTCCGCGCCACGCGTCTCCCTGGACAAGGTGATTGA
AACCATGTACGAAACCGGCAAAGACATCAACGCCAAATACCGGGAAACCTCTCGCGGCGGCCTGGCTATCAAGGTACAGT
GCGGCTGA

Upstream 100 bases:

>100_bases
CGCTGCGACGGCGCCCATGGGTGACACGTGACCCGCAATTGCGCCGGTCGACGACTTTACACCAGCAGCACAAGACATAG
CCTCTTTAAGGAGCTTTAGC

Downstream 100 bases:

>100_bases
GGCAACGCCGCGGCGGCGATAGCATAGCGGGGGCATTCGCCCCCGCTGTCTTACCTGCTCCCCCGCCATGGGGCTACGGA
CGCGGGTCCGGCGCGCTAGC

Product: L-serine ammonia-lyase

Products: NA

Alternate protein names: SDH 1; L-serine deaminase 1; L-SD1 [H]

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MISVFDMFKIGIGPSSSHTVGPMKAGKQFVDDLAAQGLLPSVTRVAVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDSVDI
DAIPEFIHSVESSERLPLGREGHPVIFPRDGGMVFRSDNLPLHENGMTIRAYGAGDAPLYSKTYYSIGGGFIVDEEHFGQ
QNDDGVKVPYPFHSAQALLDYCEETGLSISGLVMKNELALHSKEEIYAYFTAIWDTMQACIERGLNTEGILPGPLRVPRR
AAALRRMLVTSDKYSRDPMNVIDWVNMFALAVNEENAAGGRVVTAPTNGACGIVPAVLAYYDHFIDPVTPDVFVRYFLAS
GAIGALYKMNASISGAEVGCQGEVGVACSMAAAGLAELLGGSPVQVCIAAEIGMEHNLGLTCDPVAGQVQVPCIERNAIA
SVKAINAARMALQRTSAPRVSLDKVIETMYETGKDINAKYRETSRGGLAIKVQCG

Sequences:

>Translated_455_residues
MISVFDMFKIGIGPSSSHTVGPMKAGKQFVDDLAAQGLLPSVTRVAVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDSVDI
DAIPEFIHSVESSERLPLGREGHPVIFPRDGGMVFRSDNLPLHENGMTIRAYGAGDAPLYSKTYYSIGGGFIVDEEHFGQ
QNDDGVKVPYPFHSAQALLDYCEETGLSISGLVMKNELALHSKEEIYAYFTAIWDTMQACIERGLNTEGILPGPLRVPRR
AAALRRMLVTSDKYSRDPMNVIDWVNMFALAVNEENAAGGRVVTAPTNGACGIVPAVLAYYDHFIDPVTPDVFVRYFLAS
GAIGALYKMNASISGAEVGCQGEVGVACSMAAAGLAELLGGSPVQVCIAAEIGMEHNLGLTCDPVAGQVQVPCIERNAIA
SVKAINAARMALQRTSAPRVSLDKVIETMYETGKDINAKYRETSRGGLAIKVQCG
>Mature_455_residues
MISVFDMFKIGIGPSSSHTVGPMKAGKQFVDDLAAQGLLPSVTRVAVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDSVDI
DAIPEFIHSVESSERLPLGREGHPVIFPRDGGMVFRSDNLPLHENGMTIRAYGAGDAPLYSKTYYSIGGGFIVDEEHFGQ
QNDDGVKVPYPFHSAQALLDYCEETGLSISGLVMKNELALHSKEEIYAYFTAIWDTMQACIERGLNTEGILPGPLRVPRR
AAALRRMLVTSDKYSRDPMNVIDWVNMFALAVNEENAAGGRVVTAPTNGACGIVPAVLAYYDHFIDPVTPDVFVRYFLAS
GAIGALYKMNASISGAEVGCQGEVGVACSMAAAGLAELLGGSPVQVCIAAEIGMEHNLGLTCDPVAGQVQVPCIERNAIA
SVKAINAARMALQRTSAPRVSLDKVIETMYETGKDINAKYRETSRGGLAIKVQCG

Specific function: Deaminates also threonine, particularly when it is present in high concentration [H]

COG id: COG1760

COG function: function code E; L-serine deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-sulfur dependent L-serine dehydratase family [H]

Homologues:

Organism=Escherichia coli, GI1788116, Length=454, Percent_Identity=83.4801762114538, Blast_Score=780, Evalue=0.0,
Organism=Escherichia coli, GI1789161, Length=452, Percent_Identity=76.7699115044248, Blast_Score=714, Evalue=0.0,
Organism=Escherichia coli, GI48994925, Length=455, Percent_Identity=72.7472527472527, Blast_Score=668, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004644
- InterPro:   IPR005130
- InterPro:   IPR005131 [H]

Pfam domain/function: PF03313 SDH_alpha; PF03315 SDH_beta [H]

EC number: =4.3.1.17 [H]

Molecular weight: Translated: 48545; Mature: 48545

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISVFDMFKIGIGPSSSHTVGPMKAGKQFVDDLAAQGLLPSVTRVAVDVYGSLSLTGKGH
CCCCEEEHEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEEEECCCCC
HTDIAIIMGLAGNLPDSVDIDAIPEFIHSVESSERLPLGREGHPVIFPRDGGMVFRSDNL
CCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCEEEECCCC
PLHENGMTIRAYGAGDAPLYSKTYYSIGGGFIVDEEHFGQQNDDGVKVPYPFHSAQALLD
CEECCCCEEEEECCCCCCCCCCEEEECCCCEEEEHHHCCCCCCCCEECCCCCHHHHHHHH
YCEETGLSISGLVMKNELALHSKEEIYAYFTAIWDTMQACIERGLNTEGILPGPLRVPRR
HHHHCCCCEEEEEEECHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHH
AAALRRMLVTSDKYSRDPMNVIDWVNMFALAVNEENAAGGRVVTAPTNGACGIVPAVLAY
HHHHHHHHHCCCCCCCCCHHHHHHHHHHEEEECCCCCCCCEEEECCCCCCCHHHHHHHHH
YDHFIDPVTPDVFVRYFLASGAIGALYKMNASISGAEVGCQGEVGVACSMAAAGLAELLG
HHHHCCCCCHHHHHHHHHHCCCHHEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
GSPVQVCIAAEIGMEHNLGLTCDPVAGQVQVPCIERNAIASVKAINAARMALQRTSAPRV
CCCEEEEEEEECCCCCCCCEEECCCCCCEECCEECCCHHHHHHHHHHHHHHHHHCCCCCC
SLDKVIETMYETGKDINAKYRETSRGGLAIKVQCG
CHHHHHHHHHHCCCCCCCHHHCCCCCCEEEEEECC
>Mature Secondary Structure
MISVFDMFKIGIGPSSSHTVGPMKAGKQFVDDLAAQGLLPSVTRVAVDVYGSLSLTGKGH
CCCCEEEHEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEEEECCCCC
HTDIAIIMGLAGNLPDSVDIDAIPEFIHSVESSERLPLGREGHPVIFPRDGGMVFRSDNL
CCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCEEEECCCC
PLHENGMTIRAYGAGDAPLYSKTYYSIGGGFIVDEEHFGQQNDDGVKVPYPFHSAQALLD
CEECCCCEEEEECCCCCCCCCCEEEECCCCEEEEHHHCCCCCCCCEECCCCCHHHHHHHH
YCEETGLSISGLVMKNELALHSKEEIYAYFTAIWDTMQACIERGLNTEGILPGPLRVPRR
HHHHCCCCEEEEEEECHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHH
AAALRRMLVTSDKYSRDPMNVIDWVNMFALAVNEENAAGGRVVTAPTNGACGIVPAVLAY
HHHHHHHHHCCCCCCCCCHHHHHHHHHHEEEECCCCCCCCEEEECCCCCCCHHHHHHHHH
YDHFIDPVTPDVFVRYFLASGAIGALYKMNASISGAEVGCQGEVGVACSMAAAGLAELLG
HHHHCCCCCHHHHHHHHHHCCCHHEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
GSPVQVCIAAEIGMEHNLGLTCDPVAGQVQVPCIERNAIASVKAINAARMALQRTSAPRV
CCCEEEEEEEECCCCCCCCEEECCCCCCEECCEECCCHHHHHHHHHHHHHHHHHCCCCCC
SLDKVIETMYETGKDINAKYRETSRGGLAIKVQCG
CHHHHHHHHHHCCCCCCCHHHCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2504697; 8436113; 9097040; 9278503 [H]