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The map label for this gene is pykF [H]

Identifier: 238919742

GI number: 238919742

Start: 1782711

End: 1784123

Strand: Reverse

Name: pykF [H]

Synonym: NT01EI_1846

Alternate gene names: 238919742

Gene position: 1784123-1782711 (Counterclockwise)

Preceding gene: 238919744

Following gene: 238919741

Centisome position: 46.8

GC content: 57.96

Gene sequence:

>1413_bases
ATGAAAAAGACCAAAATCGTATGCACCATCGGTCCGAAGACCGAATCCGAAGAGATGCTGGGTAAACTGCTGAACGCAGG
TATGAACGTAATGCGTCTGAACTTTTCCCACGGTGACTATGAAGAACACGGCCAGCGCATCAAAAACCTGCGCGCTGTCA
TGGAGAATACCGGCCAGAAAGCCGCTATCCTGCTGGATACCAAAGGACCGGAAATCCGCACCATGAAGCTGGAAGGCGGC
AATGATGTCTCCCTGACCGCTGGCCAGACCTTCACCTTCACCACCGACCAGAGAGTCATCGGTAACAACGAACGCGTCGC
CGTCACCTATCCGGGTTTCGCCGCTGACCTGCGCATCGGCAACACCGTGCTGGTGGACGATGGCCTGATCGGCATGGAAG
TGACCGATGTCACCGAACGTACCGTGGTATGCAAGGTTCTGAACAATGGCGACCTGGGCGAGAACAAAGGTGTTAACCTG
CCGGGCGTTTCCATCCAGCTGCCGGCGCTGGCTGAAAAAGACAAGCGCGACCTGATTTTTGGCTGCGAGCAGGGCGTTGA
CTTCGTCGCCGCCTCTTTCATCCGTAAGCGCGCCGACGTCCTGGAGATCCGTGAGCACCTGAAGGCCCACGGCGGCGAGC
AGATCCAGATCATCTCCAAGATCGAAAACCAGGAAGGTCTGAACAACTTTGACGAAATTCTGGAGGCCTCCGACGGCATC
ATGGTTGCCCGTGGCGATCTGGGCGTGGAAATCCCGGTAGAAGAAGTTATCTTCGCGCAGAAGATGATGATCGAAAAATG
CAACCGTGCCCGCAAAGTGGTCATCACCGCGACCCAGATGCTGGACTCCATGATCAAAAACCCGCGCCCAACCCGTGCGG
AAGCCGGTGACGTTGCCAACGCCATCCTCGACGGCACCGACGCCGTTATGCTGTCCGGCGAGAGCGCCAAGGGTAAATAC
CCGCTGGAGTCCGTCGGTATCATGGCGACCATCTGTGAGCGTACCGACCGTGTCATGCAGAGCCGTATCGATACCCTGCA
CGACTCACGCAAGCTGCGTATCACCGAAGCAGTATGCCGCGGTGCGGTAGAGACCGCCGAAAAGCTGGATGCCCCGCTGA
TCGTCGTTGCCACCGCCGGCGGCAAATCCGCCAAAGCCGTCCGTAAGTACTTCCCGGATGCGATGATCCTGGCGCTGACC
ACTAACCCGGTCACCGCCCGCCAGCTGATCCTGAGCAAAGGCGTTATCCCTATGATGGTCAAAGAGATCGCCTCTACCGA
CGATTTCTACCGTATCGGTAAAGATGCTGCCCAGGAAAGCGGTCTGGCCCAGAAAGGCGACGTTGTCGTCATGGTCTCCG
GCGCGCTGGTACCGAGCGGCACCACCAACACCGCATCAGTACACGTGCTCTGA

Upstream 100 bases:

>100_bases
GTTCTATTTTCACATCACCTGTTTTACCCCCTGTCCTCCCAGTCGCTGGCGGTGGGAGTAAGCTTTTTCCAATCCATCAC
AACTTGCAGAAATGAATGTC

Downstream 100 bases:

>100_bases
TAGAGAGCAAAAAGATAATTAAATTCAAGCGCCGCTCTGCGGCGCTTTTTTTATCCTGTCACAACATTTTTATCTCTTGA
ACTGATATTATTAATAGAAT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK-1 [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MKKTKIVCTIGPKTESEEMLGKLLNAGMNVMRLNFSHGDYEEHGQRIKNLRAVMENTGQKAAILLDTKGPEIRTMKLEGG
NDVSLTAGQTFTFTTDQRVIGNNERVAVTYPGFAADLRIGNTVLVDDGLIGMEVTDVTERTVVCKVLNNGDLGENKGVNL
PGVSIQLPALAEKDKRDLIFGCEQGVDFVAASFIRKRADVLEIREHLKAHGGEQIQIISKIENQEGLNNFDEILEASDGI
MVARGDLGVEIPVEEVIFAQKMMIEKCNRARKVVITATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKY
PLESVGIMATICERTDRVMQSRIDTLHDSRKLRITEAVCRGAVETAEKLDAPLIVVATAGGKSAKAVRKYFPDAMILALT
TNPVTARQLILSKGVIPMMVKEIASTDDFYRIGKDAAQESGLAQKGDVVVMVSGALVPSGTTNTASVHVL

Sequences:

>Translated_470_residues
MKKTKIVCTIGPKTESEEMLGKLLNAGMNVMRLNFSHGDYEEHGQRIKNLRAVMENTGQKAAILLDTKGPEIRTMKLEGG
NDVSLTAGQTFTFTTDQRVIGNNERVAVTYPGFAADLRIGNTVLVDDGLIGMEVTDVTERTVVCKVLNNGDLGENKGVNL
PGVSIQLPALAEKDKRDLIFGCEQGVDFVAASFIRKRADVLEIREHLKAHGGEQIQIISKIENQEGLNNFDEILEASDGI
MVARGDLGVEIPVEEVIFAQKMMIEKCNRARKVVITATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKY
PLESVGIMATICERTDRVMQSRIDTLHDSRKLRITEAVCRGAVETAEKLDAPLIVVATAGGKSAKAVRKYFPDAMILALT
TNPVTARQLILSKGVIPMMVKEIASTDDFYRIGKDAAQESGLAQKGDVVVMVSGALVPSGTTNTASVHVL
>Mature_470_residues
MKKTKIVCTIGPKTESEEMLGKLLNAGMNVMRLNFSHGDYEEHGQRIKNLRAVMENTGQKAAILLDTKGPEIRTMKLEGG
NDVSLTAGQTFTFTTDQRVIGNNERVAVTYPGFAADLRIGNTVLVDDGLIGMEVTDVTERTVVCKVLNNGDLGENKGVNL
PGVSIQLPALAEKDKRDLIFGCEQGVDFVAASFIRKRADVLEIREHLKAHGGEQIQIISKIENQEGLNNFDEILEASDGI
MVARGDLGVEIPVEEVIFAQKMMIEKCNRARKVVITATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKY
PLESVGIMATICERTDRVMQSRIDTLHDSRKLRITEAVCRGAVETAEKLDAPLIVVATAGGKSAKAVRKYFPDAMILALT
TNPVTARQLILSKGVIPMMVKEIASTDDFYRIGKDAAQESGLAQKGDVVVMVSGALVPSGTTNTASVHVL

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=486, Percent_Identity=47.5308641975309, Blast_Score=405, Evalue=1e-113,
Organism=Homo sapiens, GI33286420, Length=486, Percent_Identity=47.5308641975309, Blast_Score=405, Evalue=1e-113,
Organism=Homo sapiens, GI33286418, Length=487, Percent_Identity=47.4332648870637, Blast_Score=403, Evalue=1e-112,
Organism=Homo sapiens, GI10835121, Length=486, Percent_Identity=46.0905349794239, Blast_Score=393, Evalue=1e-109,
Organism=Homo sapiens, GI32967597, Length=486, Percent_Identity=46.0905349794239, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI310128732, Length=288, Percent_Identity=50.3472222222222, Blast_Score=260, Evalue=2e-69,
Organism=Homo sapiens, GI310128730, Length=288, Percent_Identity=50.3472222222222, Blast_Score=260, Evalue=2e-69,
Organism=Homo sapiens, GI310128736, Length=236, Percent_Identity=44.9152542372881, Blast_Score=188, Evalue=1e-47,
Organism=Homo sapiens, GI310128734, Length=236, Percent_Identity=44.9152542372881, Blast_Score=188, Evalue=1e-47,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=44.6511627906977, Blast_Score=167, Evalue=2e-41,
Organism=Escherichia coli, GI1787965, Length=470, Percent_Identity=87.2340425531915, Blast_Score=837, Evalue=0.0,
Organism=Escherichia coli, GI1788160, Length=481, Percent_Identity=36.7983367983368, Blast_Score=270, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI17544584, Length=480, Percent_Identity=42.0833333333333, Blast_Score=367, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI71984406, Length=482, Percent_Identity=45.643153526971, Blast_Score=352, Evalue=2e-97,
Organism=Caenorhabditis elegans, GI71984413, Length=482, Percent_Identity=45.643153526971, Blast_Score=352, Evalue=2e-97,
Organism=Caenorhabditis elegans, GI17506829, Length=482, Percent_Identity=45.643153526971, Blast_Score=352, Evalue=2e-97,
Organism=Caenorhabditis elegans, GI17506831, Length=480, Percent_Identity=45.8333333333333, Blast_Score=352, Evalue=2e-97,
Organism=Saccharomyces cerevisiae, GI6319279, Length=481, Percent_Identity=44.9064449064449, Blast_Score=351, Evalue=1e-97,
Organism=Saccharomyces cerevisiae, GI6324923, Length=483, Percent_Identity=42.6501035196687, Blast_Score=338, Evalue=8e-94,
Organism=Drosophila melanogaster, GI24648964, Length=479, Percent_Identity=48.4342379958246, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI28571814, Length=479, Percent_Identity=48.4342379958246, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24648966, Length=446, Percent_Identity=45.9641255605381, Blast_Score=342, Evalue=3e-94,
Organism=Drosophila melanogaster, GI24581235, Length=482, Percent_Identity=36.3070539419087, Blast_Score=290, Evalue=2e-78,
Organism=Drosophila melanogaster, GI24646914, Length=274, Percent_Identity=46.3503649635037, Blast_Score=214, Evalue=1e-55,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 50810; Mature: 50810

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTKIVCTIGPKTESEEMLGKLLNAGMNVMRLNFSHGDYEEHGQRIKNLRAVMENTGQK
CCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCE
AAILLDTKGPEIRTMKLEGGNDVSLTAGQTFTFTTDQRVIGNNERVAVTYPGFAADLRIG
EEEEEECCCCCEEEEEECCCCEEEEECCCEEEEECCCCEECCCCEEEEECCCCEEEEEEC
NTVLVDDGLIGMEVTDVTERTVVCKVLNNGDLGENKGVNLPGVSIQLPALAEKDKRDLIF
CEEEEECCEECEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCEEECCCCCCCCCCCEEE
GCEQGVDFVAASFIRKRADVLEIREHLKAHGGEQIQIISKIENQEGLNNFDEILEASDGI
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHCCCCCCCHHHHHHHCCCCE
MVARGDLGVEIPVEEVIFAQKMMIEKCNRARKVVITATQMLDSMIKNPRPTRAEAGDVAN
EEEECCCCCCCCHHHHHHHHHHHHHHHCCHHEEEEEHHHHHHHHHCCCCCCCCCHHHHHH
AILDGTDAVMLSGESAKGKYPLESVGIMATICERTDRVMQSRIDTLHDSRKLRITEAVCR
HHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHH
GAVETAEKLDAPLIVVATAGGKSAKAVRKYFPDAMILALTTNPVTARQLILSKGVIPMMV
HHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCEEEEEEECCCHHHHHHHHHCCCHHHHH
KEIASTDDFYRIGKDAAQESGLAQKGDVVVMVSGALVPSGTTNTASVHVL
HHHHCCCHHHHHCHHHHHHCCCCCCCCEEEEEECEECCCCCCCCCEEEEC
>Mature Secondary Structure
MKKTKIVCTIGPKTESEEMLGKLLNAGMNVMRLNFSHGDYEEHGQRIKNLRAVMENTGQK
CCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCE
AAILLDTKGPEIRTMKLEGGNDVSLTAGQTFTFTTDQRVIGNNERVAVTYPGFAADLRIG
EEEEEECCCCCEEEEEECCCCEEEEECCCEEEEECCCCEECCCCEEEEECCCCEEEEEEC
NTVLVDDGLIGMEVTDVTERTVVCKVLNNGDLGENKGVNLPGVSIQLPALAEKDKRDLIF
CEEEEECCEECEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCEEECCCCCCCCCCCEEE
GCEQGVDFVAASFIRKRADVLEIREHLKAHGGEQIQIISKIENQEGLNNFDEILEASDGI
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHCCCCCCCHHHHHHHCCCCE
MVARGDLGVEIPVEEVIFAQKMMIEKCNRARKVVITATQMLDSMIKNPRPTRAEAGDVAN
EEEECCCCCCCCHHHHHHHHHHHHHHHCCHHEEEEEHHHHHHHHHCCCCCCCCCHHHHHH
AILDGTDAVMLSGESAKGKYPLESVGIMATICERTDRVMQSRIDTLHDSRKLRITEAVCR
HHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHH
GAVETAEKLDAPLIVVATAGGKSAKAVRKYFPDAMILALTTNPVTARQLILSKGVIPMMV
HHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCEEEEEEECCCHHHHHHHHHCCCHHHHH
KEIASTDDFYRIGKDAAQESGLAQKGDVVVMVSGALVPSGTTNTASVHVL
HHHHCCCHHHHHCHHHHHHCCCCCCCCEEEEEECEECCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]