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The map label for this gene is cysB [H]

Identifier: 238919615

GI number: 238919615

Start: 1670834

End: 1671808

Strand: Direct

Name: cysB [H]

Synonym: NT01EI_1714

Alternate gene names: 238919615

Gene position: 1670834-1671808 (Clockwise)

Preceding gene: 238919610

Following gene: 238919616

Centisome position: 43.83

GC content: 56.21

Gene sequence:

>975_bases
ATGAAATTGCAGCAGCTTCGTTATATTGTTGAAGTTGTTAACCACAACCTTAACGTCTCTTCTACCGCCGAGGGGCTTTA
TACCTCACAGCCCGGTATCAGCAAGCAGGTGCGTATGCTGGAGGATGAGCTGGGGGTTCAGATATTTTCGCGCAGTGGCA
AGCACCTGACTCAGGTAACGCCGGCAGGACAAGCGATTATCCGGATTGCTCGCGAGGTGCTGTCCAAAGTCGATGCCATC
AAGGCCGTAGCCGGCGAGCATACCTACCCCGATCAGGGGTCGCTGTATGTCGCGACCACCCACACGCAGGCCCGCTATGC
CCTTCCTGCGGTGATCCAGAATTTTATCAAGCGCTATCCACGGGTGTCGCTGCACATGCATCAGGGATCGCCGAGTCAAA
TTGCCGAGGCCGTTTGTAAGGGGAACGCCGATTTCGCCATCGCGACGGAGGCGCTGCACCTGTATGCCGATCTGGTGATG
CTACCCTGCTATCACTGGAATCGTGCGCTGGTGGTGATGCCGAACCACCCGTTGGCCGGGCGCGATCAGGTCAGCATTGA
AGAGCTGGCGGAGTACCCCTTGGTGACCTATACCTTTGGCTTTACCGGGCGCTCCACGCTGGACGGTGCCTTCAATCAGG
CGGGGCTGACGCCGCGTATCGTCTTTACCGCAACGGATGCAGATGTGATTAAGACCTATGTGCGGCTGGGGCTTGGGGTG
GGGGTGATAGCCGGGATGGCTCTTGACCCGCAGATTGATGGTGATCTGGTCGCGTTGGACGCCAGCCATCTGTTTGGTTA
CAGTACCACGAAGATAGGTTTTCGCCGTAGTACTTTCCTGCGCAGCTATATGTACGATTTTATTGCCCGCTTTGCGCCCC
ACCTTACGCGGGAGGTCATTGATGCCGCGCTGGCGTTGCGCAGTAATGAGGAAATAGAGGCGATGTTTCAGGATATCGAG
TTACCGGTAAAATAA

Upstream 100 bases:

>100_bases
TAATGATATAGTGGTTATATAAAACCCATTTTCTGGTTTTTAATGTTATTTGCATGCCATGCGTCGCAATAACCAATGAT
AACTTAGGATGGCACAGGCT

Downstream 100 bases:

>100_bases
TCGGTGAATTTGACGTCTGTCGCTGCCTTTGTGGCGGATGCCAATTTGTCCAATGCCGTCGGGTATTTTTTTATTTCGTC
GTCTGTCCTTTGCGGCTTTG

Product: HTH-type transcriptional regulator CysB

Products: NA

Alternate protein names: Cys regulon transcriptional activator [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDELGVQIFSRSGKHLTQVTPAGQAIIRIAREVLSKVDAI
KAVAGEHTYPDQGSLYVATTHTQARYALPAVIQNFIKRYPRVSLHMHQGSPSQIAEAVCKGNADFAIATEALHLYADLVM
LPCYHWNRALVVMPNHPLAGRDQVSIEELAEYPLVTYTFGFTGRSTLDGAFNQAGLTPRIVFTATDADVIKTYVRLGLGV
GVIAGMALDPQIDGDLVALDASHLFGYSTTKIGFRRSTFLRSYMYDFIARFAPHLTREVIDAALALRSNEEIEAMFQDIE
LPVK

Sequences:

>Translated_324_residues
MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDELGVQIFSRSGKHLTQVTPAGQAIIRIAREVLSKVDAI
KAVAGEHTYPDQGSLYVATTHTQARYALPAVIQNFIKRYPRVSLHMHQGSPSQIAEAVCKGNADFAIATEALHLYADLVM
LPCYHWNRALVVMPNHPLAGRDQVSIEELAEYPLVTYTFGFTGRSTLDGAFNQAGLTPRIVFTATDADVIKTYVRLGLGV
GVIAGMALDPQIDGDLVALDASHLFGYSTTKIGFRRSTFLRSYMYDFIARFAPHLTREVIDAALALRSNEEIEAMFQDIE
LPVK
>Mature_324_residues
MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDELGVQIFSRSGKHLTQVTPAGQAIIRIAREVLSKVDAI
KAVAGEHTYPDQGSLYVATTHTQARYALPAVIQNFIKRYPRVSLHMHQGSPSQIAEAVCKGNADFAIATEALHLYADLVM
LPCYHWNRALVVMPNHPLAGRDQVSIEELAEYPLVTYTFGFTGRSTLDGAFNQAGLTPRIVFTATDADVIKTYVRLGLGV
GVIAGMALDPQIDGDLVALDASHLFGYSTTKIGFRRSTFLRSYMYDFIARFAPHLTREVIDAALALRSNEEIEAMFQDIE
LPVK

Specific function: This protein is a positive regulator of gene expression for the cysteine regulon. The inducer for cysB is N-acetylserine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787530, Length=324, Percent_Identity=85.8024691358025, Blast_Score=586, Evalue=1e-169,
Organism=Escherichia coli, GI1788296, Length=313, Percent_Identity=40.8945686900958, Blast_Score=247, Evalue=6e-67,
Organism=Escherichia coli, GI157672245, Length=235, Percent_Identity=30.6382978723404, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI1790399, Length=283, Percent_Identity=26.5017667844523, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788887, Length=252, Percent_Identity=28.1746031746032, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790262, Length=252, Percent_Identity=26.1904761904762, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1788481, Length=254, Percent_Identity=25.1968503937008, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI145693105, Length=229, Percent_Identity=22.2707423580786, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1787879, Length=252, Percent_Identity=26.984126984127, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789204, Length=264, Percent_Identity=26.1363636363636, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1787601, Length=267, Percent_Identity=24.3445692883895, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI2367136, Length=246, Percent_Identity=23.9837398373984, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790208, Length=260, Percent_Identity=25.3846153846154, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1788748, Length=246, Percent_Identity=23.5772357723577, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1787806, Length=148, Percent_Identity=27.7027027027027, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35811; Mature: 35811

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDELGVQIFSRSGKHLTQVT
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHCCCCEEEEEC
PAGQAIIRIAREVLSKVDAIKAVAGEHTYPDQGSLYVATTHTQARYALPAVIQNFIKRYP
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCC
RVSLHMHQGSPSQIAEAVCKGNADFAIATEALHLYADLVMLPCYHWNRALVVMPNHPLAG
CEEEEEECCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCEECCCEEEEECCCCCCC
RDQVSIEELAEYPLVTYTFGFTGRSTLDGAFNQAGLTPRIVFTATDADVIKTYVRLGLGV
CCCCCHHHHHCCCEEEEEECCCCCCHHCCCHHCCCCCEEEEEEECCHHHHHHHHHHHHHH
GVIAGMALDPQIDGDLVALDASHLFGYSTTKIGFRRSTFLRSYMYDFIARFAPHLTREVI
HHHHCCEECCCCCCCEEEEEHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DAALALRSNEEIEAMFQDIELPVK
HHHHHHCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDELGVQIFSRSGKHLTQVT
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHCCCCEEEEEC
PAGQAIIRIAREVLSKVDAIKAVAGEHTYPDQGSLYVATTHTQARYALPAVIQNFIKRYP
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCC
RVSLHMHQGSPSQIAEAVCKGNADFAIATEALHLYADLVMLPCYHWNRALVVMPNHPLAG
CEEEEEECCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCEECCCEEEEECCCCCCC
RDQVSIEELAEYPLVTYTFGFTGRSTLDGAFNQAGLTPRIVFTATDADVIKTYVRLGLGV
CCCCCHHHHHCCCEEEEEECCCCCCHHCCCHHCCCCCEEEEEEECCHHHHHHHHHHHHHH
GVIAGMALDPQIDGDLVALDASHLFGYSTTKIGFRRSTFLRSYMYDFIARFAPHLTREVI
HHHHCCEECCCCCCCEEEEEHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DAALALRSNEEIEAMFQDIELPVK
HHHHHHCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]