Click here to switch to the map view.

The map label for this gene is topA [H]

Identifier: 238919610

GI number: 238919610

Start: 1665327

End: 1667927

Strand: Direct

Name: topA [H]

Synonym: NT01EI_1709

Alternate gene names: 238919610

Gene position: 1665327-1667927 (Clockwise)

Preceding gene: 238919608

Following gene: 238919615

Centisome position: 43.68

GC content: 58.05

Gene sequence:

>2601_bases
ATGGGTAAAGCTCTCGTTATAGTTGAGTCCCCGGCAAAGGCCAAAACCATAAATAAGTATCTCGGCAATGACTACGTGGT
GAAGTCCAGCGTCGGTCATATCCGCGATCTGCCGACCAGTGGCTCAGCCAGTAAGAAGGGCGCGGCCTCGGCCGGCGATA
AAGCGAAGACGAAGGCCAAAAAGAATGAGCGTGATGCGTTGGTCAATCGTATGGGGGTCGATCCCTACCATGGTTGGCAG
GCACATTACGAAGTTCTGCCGGGCAAAGAGAAGGTGGTGTCTGAGCTAAAGGCCCTGGCCGAGAAAGCCGATCACATTTA
TCTGGCAACCGATCTTGACCGCGAAGGAGAGGCCATCGCATGGCATCTGCGCGAGGTGATTGGCGGTGATGGCAGCCGCT
ATAGTCGCGTGGTGTTTAACGAAATTACCAAAAACGCCATTCAGCAGGCATTCAGTCAGCCCGGTGAACTGAATATCGAT
CGCGTCAATGCCCAGCAGGCGCGTCGTTTCATGGATCGCGTGGTGGGCTATATGGTCTCGCCACTGCTGTGGAAAAAGGT
TGCCCGGGGCCTCTCGGCGGGTCGAGTCCAGTCCGTCGCCGTACGGTTGATCGTTGAGCGGGAGCGTGAAATTAAGGCCT
TTGTGCCGGAAGAGTATTGGGAGTTGCGGGCCGATCTCGCCGTTGGTGCAGATGTCAGCTTTCAGGCTCAGGTGACCCAC
TACAACGATAAACCATTTAAACCGGTCAACGCCGCTCAAAGTCAGGCGGCCGTTACGGCGTTGGAGAACGCCCGTTTCGC
GGTGCGCGAGCGTGAGGATAAGCCGACCAGCAGCAAGCCGTCTGCGCCCTTTATTACCTCGACCCTGCAGCAGGCGGCCA
GTACCCGCCTTGGCTTTGGGGTGAAGAAAACCATGATGATGGCCCAGCGTCTGTACGAGGCAGGATACATTACCTATATG
CGTACCGACTCGACCAACCTGAGTCAGGATGCCCTGAATATGGTACGGGCCTACATCGGTGATAATTTTGGCGCGGAGTA
CCTACCGACAGACGCCAACGTCTACGCCAGCAAGGATAACTCCCAAGAGGCTCATGAGGCGATCCGTCCGTCGGATGTCA
GCGTGCTGGCCGACGATCTCAAGGGGATGGAGGCCGATGCGCTGAAGCTGTATCAGCTGATCTGGCGCCAGTTTGTCTCC
TGCCAGATGACGGCAGCCAAGTATGATGCTAGTACCATTACCGTGCAGGCCGCGGATTATACCCTGAAGGCGCGCGGACG
TACCCTGCGGTTTGATGGTTGGACCCGGGTGCTGCCGCAGCTGCGTAAGGGGGATGAAGATCGCGCCCTGCCGGCGGTGG
AGAGCGGGCGGGCGCTGGATCTGCTGCAGCTGCAGCCCAGCCAGCACTTTACCAAGCCGCCGGCCCGTTTCAGCGAAGCC
TCGCTGGTACGAGAGCTGGAGAAGCGTGGTATCGGCCGCCCGTCAACCTATGCGGCGATTATCTCGACTATCCAGGATCG
CGGCTATGTGCGCACCGATAACCGTCGCTTCTACGCGGAAAAGATGGGGGAGATCGTGACCGATCGCCTGGAGGAGAACT
TCCGCGAGCTGATGAGCTATGACTTTACCGCGCACATGGAAGATGGCCTGGACCGCGTCGCCAGTGCTCAGGCTGACTGG
AAGGTGATGCTGGACGGCTTTTTCAGCGACTTCAGTCAACAGTTGGAGGTGGCGGAGAAGCCGCCGGAGGAGGGGGGGAT
GCGTCCCAATCCGATGGTGCTGACCTGCATTGCCTGCCCGACCTGTGAGCGGCAGATGGGGATACGTACTGCCAGCACCG
GCGTTTTCCTGGGATGCTCTGGCTATACACTGTCGCCCAAAGAGCGCTGTAAAACCACCATCAATCTGGTGCCGGAAGCT
GAAGTACTTAATGTGCTGGAGGGCGATGATGCGGAAACCAACGCCCTGCGCGCGAAGCGACGCTGCCCGAAATGCGGTAC
GGCAATGGACAGCTACCTGATCGATAACCACCGTAAGCTGCATGTGTGTGGCAATAACCCCGCCTGCGATGGCTATGAAG
TGGAAGAGGGTGAGTTTCGTATTAAGGGATATGACGGCCCGATCGTAGAGTGTGATAAGTGCGGTGCCGAGATGCATCTG
AAGATGGGACGCTTTGGCAAGTATATGGGGTGCACCAACGAAGCCTGTAAAAACACCCGTAAGATCCTGCGCAACGGCGA
GGTAGCACCGCCGAAGGAGGATCCGGTGCCGCTGCCGGAGCTGGCGTGTGAGAAGTCAGACGCCTATTTCGTGCTGCGTG
ATGGCGCCGCGGGGGTGTTCCTGGCGGCCAACACCTTCCCTAAATCACGTGAAACCCGTGCCCCGCTGGTGGAAGAACTG
TACCGCTTCCGCGATCGTCTGCCGGAAAAACTGCGCTATCTGGCGGAGGCTCCGCAGCAGGATGCCCAGGGTAATGCCAC
GCTGGTTCGCTTTAGCCGGAAAACCAAGCAGCAGTATGTTTCCAGCGAGAAGGACGGCAAGGCGACGGGCTGGACCGCGT
TCTTTATTGATGGAAAGTGGGTCGTATCAGAGAAAAAGTAA

Upstream 100 bases:

>100_bases
GGCGTCTGTCGCAATATACAAAAGAGTTCTATACAGTGCCGGCTTAATGGCGAATTAAAGGCTTCTGGCAACAGAAGGAT
GCATTCAGGTAAAGAGAATT

Downstream 100 bases:

>100_bases
TCCCGCAGGGAGATGCTTCTATGCCGGCCTTTAGGCCGGCATTTTTATTCCTGCATAAACACCACCGCCATAGGAGGTGG
TCAGCAATAGGTTGGCTCTG

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 866; Mature: 865

Protein sequence:

>866_residues
MGKALVIVESPAKAKTINKYLGNDYVVKSSVGHIRDLPTSGSASKKGAASAGDKAKTKAKKNERDALVNRMGVDPYHGWQ
AHYEVLPGKEKVVSELKALAEKADHIYLATDLDREGEAIAWHLREVIGGDGSRYSRVVFNEITKNAIQQAFSQPGELNID
RVNAQQARRFMDRVVGYMVSPLLWKKVARGLSAGRVQSVAVRLIVEREREIKAFVPEEYWELRADLAVGADVSFQAQVTH
YNDKPFKPVNAAQSQAAVTALENARFAVREREDKPTSSKPSAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYM
RTDSTNLSQDALNMVRAYIGDNFGAEYLPTDANVYASKDNSQEAHEAIRPSDVSVLADDLKGMEADALKLYQLIWRQFVS
CQMTAAKYDASTITVQAADYTLKARGRTLRFDGWTRVLPQLRKGDEDRALPAVESGRALDLLQLQPSQHFTKPPARFSEA
SLVRELEKRGIGRPSTYAAIISTIQDRGYVRTDNRRFYAEKMGEIVTDRLEENFRELMSYDFTAHMEDGLDRVASAQADW
KVMLDGFFSDFSQQLEVAEKPPEEGGMRPNPMVLTCIACPTCERQMGIRTASTGVFLGCSGYTLSPKERCKTTINLVPEA
EVLNVLEGDDAETNALRAKRRCPKCGTAMDSYLIDNHRKLHVCGNNPACDGYEVEEGEFRIKGYDGPIVECDKCGAEMHL
KMGRFGKYMGCTNEACKNTRKILRNGEVAPPKEDPVPLPELACEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVEEL
YRFRDRLPEKLRYLAEAPQQDAQGNATLVRFSRKTKQQYVSSEKDGKATGWTAFFIDGKWVVSEKK

Sequences:

>Translated_866_residues
MGKALVIVESPAKAKTINKYLGNDYVVKSSVGHIRDLPTSGSASKKGAASAGDKAKTKAKKNERDALVNRMGVDPYHGWQ
AHYEVLPGKEKVVSELKALAEKADHIYLATDLDREGEAIAWHLREVIGGDGSRYSRVVFNEITKNAIQQAFSQPGELNID
RVNAQQARRFMDRVVGYMVSPLLWKKVARGLSAGRVQSVAVRLIVEREREIKAFVPEEYWELRADLAVGADVSFQAQVTH
YNDKPFKPVNAAQSQAAVTALENARFAVREREDKPTSSKPSAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYM
RTDSTNLSQDALNMVRAYIGDNFGAEYLPTDANVYASKDNSQEAHEAIRPSDVSVLADDLKGMEADALKLYQLIWRQFVS
CQMTAAKYDASTITVQAADYTLKARGRTLRFDGWTRVLPQLRKGDEDRALPAVESGRALDLLQLQPSQHFTKPPARFSEA
SLVRELEKRGIGRPSTYAAIISTIQDRGYVRTDNRRFYAEKMGEIVTDRLEENFRELMSYDFTAHMEDGLDRVASAQADW
KVMLDGFFSDFSQQLEVAEKPPEEGGMRPNPMVLTCIACPTCERQMGIRTASTGVFLGCSGYTLSPKERCKTTINLVPEA
EVLNVLEGDDAETNALRAKRRCPKCGTAMDSYLIDNHRKLHVCGNNPACDGYEVEEGEFRIKGYDGPIVECDKCGAEMHL
KMGRFGKYMGCTNEACKNTRKILRNGEVAPPKEDPVPLPELACEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVEEL
YRFRDRLPEKLRYLAEAPQQDAQGNATLVRFSRKTKQQYVSSEKDGKATGWTAFFIDGKWVVSEKK
>Mature_865_residues
GKALVIVESPAKAKTINKYLGNDYVVKSSVGHIRDLPTSGSASKKGAASAGDKAKTKAKKNERDALVNRMGVDPYHGWQA
HYEVLPGKEKVVSELKALAEKADHIYLATDLDREGEAIAWHLREVIGGDGSRYSRVVFNEITKNAIQQAFSQPGELNIDR
VNAQQARRFMDRVVGYMVSPLLWKKVARGLSAGRVQSVAVRLIVEREREIKAFVPEEYWELRADLAVGADVSFQAQVTHY
NDKPFKPVNAAQSQAAVTALENARFAVREREDKPTSSKPSAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMR
TDSTNLSQDALNMVRAYIGDNFGAEYLPTDANVYASKDNSQEAHEAIRPSDVSVLADDLKGMEADALKLYQLIWRQFVSC
QMTAAKYDASTITVQAADYTLKARGRTLRFDGWTRVLPQLRKGDEDRALPAVESGRALDLLQLQPSQHFTKPPARFSEAS
LVRELEKRGIGRPSTYAAIISTIQDRGYVRTDNRRFYAEKMGEIVTDRLEENFRELMSYDFTAHMEDGLDRVASAQADWK
VMLDGFFSDFSQQLEVAEKPPEEGGMRPNPMVLTCIACPTCERQMGIRTASTGVFLGCSGYTLSPKERCKTTINLVPEAE
VLNVLEGDDAETNALRAKRRCPKCGTAMDSYLIDNHRKLHVCGNNPACDGYEVEEGEFRIKGYDGPIVECDKCGAEMHLK
MGRFGKYMGCTNEACKNTRKILRNGEVAPPKEDPVPLPELACEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVEELY
RFRDRLPEKLRYLAEAPQQDAQGNATLVRFSRKTKQQYVSSEKDGKATGWTAFFIDGKWVVSEKK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=610, Percent_Identity=24.9180327868852, Blast_Score=139, Evalue=2e-32,
Organism=Homo sapiens, GI10835218, Length=603, Percent_Identity=24.7097844112769, Blast_Score=133, Evalue=6e-31,
Organism=Escherichia coli, GI1787529, Length=865, Percent_Identity=83.9306358381503, Blast_Score=1536, Evalue=0.0,
Organism=Escherichia coli, GI1788061, Length=555, Percent_Identity=24.1441441441441, Blast_Score=119, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI32563869, Length=566, Percent_Identity=24.0282685512367, Blast_Score=136, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17555378, Length=505, Percent_Identity=24.5544554455446, Blast_Score=119, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6323263, Length=485, Percent_Identity=25.360824742268, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24585251, Length=621, Percent_Identity=24.7987117552335, Blast_Score=133, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24640096, Length=488, Percent_Identity=25.2049180327869, Blast_Score=112, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR013263
- InterPro:   IPR006171 [H]

Pfam domain/function: PF08272 Topo_Zn_Ribbon; PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 96610; Mature: 96478

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKALVIVESPAKAKTINKYLGNDYVVKSSVGHIRDLPTSGSASKKGAASAGDKAKTKAK
CCCEEEEEECCCHHHHHHHHHCCCEEEECCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHH
KNERDALVNRMGVDPYHGWQAHYEVLPGKEKVVSELKALAEKADHIYLATDLDREGEAIA
CCHHHHHHHHCCCCCCCCCCHHEEECCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHH
WHLREVIGGDGSRYSRVVFNEITKNAIQQAFSQPGELNIDRVNAQQARRFMDRVVGYMVS
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHH
PLLWKKVARGLSAGRVQSVAVRLIVEREREIKAFVPEEYWELRADLAVGADVSFQAQVTH
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEECCCCEEEEEEEE
YNDKPFKPVNAAQSQAAVTALENARFAVREREDKPTSSKPSAPFITSTLQQAASTRLGFG
CCCCCCCCCCHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
VKKTMMMAQRLYEAGYITYMRTDSTNLSQDALNMVRAYIGDNFGAEYLPTDANVYASKDN
HHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCC
SQEAHEAIRPSDVSVLADDLKGMEADALKLYQLIWRQFVSCQMTAAKYDASTITVQAADY
CHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCE
TLKARGRTLRFDGWTRVLPQLRKGDEDRALPAVESGRALDLLQLQPSQHFTKPPARFSEA
EEECCCCEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHH
SLVRELEKRGIGRPSTYAAIISTIQDRGYVRTDNRRFYAEKMGEIVTDRLEENFRELMSY
HHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEECCCEEHHHHHHHHHHHHHHHHHHHHHHC
DFTAHMEDGLDRVASAQADWKVMLDGFFSDFSQQLEVAEKPPEEGGMRPNPMVLTCIACP
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCEEEEEEECC
TCERQMGIRTASTGVFLGCSGYTLSPKERCKTTINLVPEAEVLNVLEGDDAETNALRAKR
CCHHHHCCEEECCCEEEECCCCCCCHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHH
RCPKCGTAMDSYLIDNHRKLHVCGNNPACDGYEVEEGEFRIKGYDGPIVECDKCGAEMHL
CCCCCCCHHHHHHHCCCCEEEEECCCCCCCCEEECCCCEEEECCCCCEEEHHHCCCHHHH
KMGRFGKYMGCTNEACKNTRKILRNGEVAPPKEDPVPLPELACEKSDAYFVLRDGAAGVF
HHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCEEEEECCCCEEE
LAANTFPKSRETRAPLVEELYRFRDRLPEKLRYLAEAPQQDAQGNATLVRFSRKTKQQYV
EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHH
SSEKDGKATGWTAFFIDGKWVVSEKK
CCCCCCCCCCEEEEEEECEEEECCCC
>Mature Secondary Structure 
GKALVIVESPAKAKTINKYLGNDYVVKSSVGHIRDLPTSGSASKKGAASAGDKAKTKAK
CCEEEEEECCCHHHHHHHHHCCCEEEECCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHH
KNERDALVNRMGVDPYHGWQAHYEVLPGKEKVVSELKALAEKADHIYLATDLDREGEAIA
CCHHHHHHHHCCCCCCCCCCHHEEECCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHH
WHLREVIGGDGSRYSRVVFNEITKNAIQQAFSQPGELNIDRVNAQQARRFMDRVVGYMVS
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHH
PLLWKKVARGLSAGRVQSVAVRLIVEREREIKAFVPEEYWELRADLAVGADVSFQAQVTH
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEECCCCEEEEEEEE
YNDKPFKPVNAAQSQAAVTALENARFAVREREDKPTSSKPSAPFITSTLQQAASTRLGFG
CCCCCCCCCCHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
VKKTMMMAQRLYEAGYITYMRTDSTNLSQDALNMVRAYIGDNFGAEYLPTDANVYASKDN
HHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCC
SQEAHEAIRPSDVSVLADDLKGMEADALKLYQLIWRQFVSCQMTAAKYDASTITVQAADY
CHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCE
TLKARGRTLRFDGWTRVLPQLRKGDEDRALPAVESGRALDLLQLQPSQHFTKPPARFSEA
EEECCCCEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHH
SLVRELEKRGIGRPSTYAAIISTIQDRGYVRTDNRRFYAEKMGEIVTDRLEENFRELMSY
HHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEECCCEEHHHHHHHHHHHHHHHHHHHHHHC
DFTAHMEDGLDRVASAQADWKVMLDGFFSDFSQQLEVAEKPPEEGGMRPNPMVLTCIACP
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCEEEEEEECC
TCERQMGIRTASTGVFLGCSGYTLSPKERCKTTINLVPEAEVLNVLEGDDAETNALRAKR
CCHHHHCCEEECCCEEEECCCCCCCHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHH
RCPKCGTAMDSYLIDNHRKLHVCGNNPACDGYEVEEGEFRIKGYDGPIVECDKCGAEMHL
CCCCCCCHHHHHHHCCCCEEEEECCCCCCCCEEECCCCEEEECCCCCEEEHHHCCCHHHH
KMGRFGKYMGCTNEACKNTRKILRNGEVAPPKEDPVPLPELACEKSDAYFVLRDGAAGVF
HHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCEEEEECCCCEEE
LAANTFPKSRETRAPLVEELYRFRDRLPEKLRYLAEAPQQDAQGNATLVRFSRKTKQQYV
EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHH
SSEKDGKATGWTAFFIDGKWVVSEKK
CCCCCCCCCCEEEEEEECEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3029379; 9097039; 9278503; 3032952; 8114910; 7779808 [H]