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The map label for this gene is cobS [H]

Identifier: 238919604

GI number: 238919604

Start: 1660550

End: 1661287

Strand: Direct

Name: cobS [H]

Synonym: NT01EI_1703

Alternate gene names: 238919604

Gene position: 1660550-1661287 (Clockwise)

Preceding gene: 238919603

Following gene: 238919605

Centisome position: 43.56

GC content: 58.94

Gene sequence:

>738_bases
ATGAAACTCTTTCTTGCGACCCTGCAGTTTATGACCCGGATCCCGGTATCGCCGCGCTGCAGCGATGATCTGACCCTGAA
TGATTATCCGCGCGGCATCATCTATTTTCCCTTTGTGGGTTTGGTGATCGGGGTGATCAGCGCGCTGGCCTATGGCGTAT
TGGCGACTACCTATGGCAGCTGGCTGGGGGCGGTTGCCGCGGTGCTGGCGACGACCCTGGTGACCGGCGCCTTTCATCTC
GACGGTTTGGCGGATACCTGCGATGGGTTGTTTTCCGCCCGCCCGCGTGATCGCATGCTGGAGATCATGCGCGACAGCCG
CATTGGTACCAACGGGACGCTGGCGCTGATCTTTGTGATCCTGCTGCAGGTGCTGCTGATCCAGCATCTGGCGGGCGGCA
GTCTGCACATCTACGCCGTGCTGGTGGCGATGCCGGTGCTCAGCCGCACGTTGTTGGCCGTTATGATGTTCCGTCAGCGC
TATGCGCGCGAGAGCGGCATGGGCAATCTTTATATCGGTAAAATCAGCGGCAGTCACTATGTGCTGACCCTGGTTATCGG
CCTGCTGCTGGTGTTTGGTCTGCTGCATTGGCATGCCGTGCTGGCCGCGTTTATTACCTATCTCTTTGCCTTGATGTACC
GGGCTTTCATCAATATGCGGCTGGGGGGGCAGACCGGGGATACGCTGGGTGCGGCAAACGAGCTGTTTGCGCTGGTATTT
CTGCTGTCGCTGTACTGA

Upstream 100 bases:

>100_bases
ATAGGGGTATCAGTACGTTATTAGATCGTACCACGGCGCGTCCCCTGGATGGGCGCCGCGGGAACGATGCGGCACTTTTT
TATGGATGGCTTAACGGACA

Downstream 100 bases:

>100_bases
GTGGGTCCCATGGAGCTCTATCTGGTTCGTCACGGTGAGACTCAGGCCAATCGGGATGGCCTCTACTGCGGCAGCAGCGA
TGTGGCGCTGACGCCGCAGG

Product: cobalamin synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MKLFLATLQFMTRIPVSPRCSDDLTLNDYPRGIIYFPFVGLVIGVISALAYGVLATTYGSWLGAVAAVLATTLVTGAFHL
DGLADTCDGLFSARPRDRMLEIMRDSRIGTNGTLALIFVILLQVLLIQHLAGGSLHIYAVLVAMPVLSRTLLAVMMFRQR
YARESGMGNLYIGKISGSHYVLTLVIGLLLVFGLLHWHAVLAAFITYLFALMYRAFINMRLGGQTGDTLGAANELFALVF
LLSLY

Sequences:

>Translated_245_residues
MKLFLATLQFMTRIPVSPRCSDDLTLNDYPRGIIYFPFVGLVIGVISALAYGVLATTYGSWLGAVAAVLATTLVTGAFHL
DGLADTCDGLFSARPRDRMLEIMRDSRIGTNGTLALIFVILLQVLLIQHLAGGSLHIYAVLVAMPVLSRTLLAVMMFRQR
YARESGMGNLYIGKISGSHYVLTLVIGLLLVFGLLHWHAVLAAFITYLFALMYRAFINMRLGGQTGDTLGAANELFALVF
LLSLY
>Mature_245_residues
MKLFLATLQFMTRIPVSPRCSDDLTLNDYPRGIIYFPFVGLVIGVISALAYGVLATTYGSWLGAVAAVLATTLVTGAFHL
DGLADTCDGLFSARPRDRMLEIMRDSRIGTNGTLALIFVILLQVLLIQHLAGGSLHIYAVLVAMPVLSRTLLAVMMFRQR
YARESGMGNLYIGKISGSHYVLTLVIGLLLVFGLLHWHAVLAAFITYLFALMYRAFINMRLGGQTGDTLGAANELFALVF
LLSLY

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=246, Percent_Identity=52.4390243902439, Blast_Score=224, Evalue=5e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 26785; Mature: 26785

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHC
>Mature Secondary Structure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HHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA