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The map label for this gene is rluB [H]

Identifier: 238919603

GI number: 238919603

Start: 1659438

End: 1660313

Strand: Direct

Name: rluB [H]

Synonym: NT01EI_1702

Alternate gene names: 238919603

Gene position: 1659438-1660313 (Clockwise)

Preceding gene: 238919599

Following gene: 238919604

Centisome position: 43.53

GC content: 61.53

Gene sequence:

>876_bases
ATGAGCGAAAAATTACAGAAAGTTCTTGCCCGCGCCGGTCATGGCTCACGTCGTGAAATCGAAGCGCTGATCAAAGAGGG
TCGGGTAAGCGTGGATGGCAAGCTGGCCACGCTGGGAGATCGTGTTGAGGTGATGCCCAACATGAAAATCCGCGTTGATG
GCCGGGTGGTCTCCATCCGTGAGTCTGAGCAGGAAATCTGCCGCGTGCTGGCCTACTATAAACCGGAAGGTGAGCTGTGT
ACCCGCCGTGATCCGGAAGGCCGTCCCACGGTGTTCGATCGTCTTCCCCGTTTGCGCGGTTCGCGCTGGATTGCCGTGGG
ACGTCTGGACATCAACACCTCGGGGCTGCTGCTGTTTACCACGGACGGGGAACTGGCCAACCGCCTTATGCACCCGAGCC
GGGAGGTAGAGCGTGAGTACGCGGTACGCGTTTTCGGCCAGGTTGATGAGGACAAGCTGCGTCAGCTTCGTCGCGGCGTA
CAGCTGGAGGATGGCCCAGCCAGTTTTAAGAGCATCAGTTTCCAGGGCGGAGAGGGGCTGAATCAGTGGTACAACGTCAC
GCTGACCGAGGGACGTAACCGAGAAGTGCGTCGCTTGTGGGAAGCCGTCGGCGTACAGGTGAGCCGTCTGATCCGCGTAC
GCTACGGCGATCTGCCCTTGCCTAAGGGGTTGCCGCGCGGCGGCTGGCAGGAGCTGCCGCTGGAGAATATTAACTATCTG
CGTGAGCTGGTGGAACTGCAGGCGGAGACCGCCAGCAAGCTGCCGGTTGAGCGTGAGCGTCGCCGGATGAAAGCCAACCA
AATCCGGCGGGCGGTTAAACGCCATGGCCAGCACAGCGGCGGCAGCCGCCGTGCGCCGACCACGAAGCGCGGCTGA

Upstream 100 bases:

>100_bases
GGCCCATGCCCGGCAGAAGATACGCCTGTTTTAGTACCGGTCGCCGCGGCGGCCGGTCAACAATAACGACGCCTGTGAAG
GCGACAGCTGAGGTTGCTCA

Downstream 100 bases:

>100_bases
GCCTGTCGCGCTAAACCGTCCTTCGGGGCGGTTTTTTTATGTTTTTCGCTCAGCGAGTGGGTTAGCTGAGCGGGCGGCTT
ATCTCAGGTATTTTCCTCTG

Product: 23S rRNA pseudouridylate synthase B

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MSEKLQKVLARAGHGSRREIEALIKEGRVSVDGKLATLGDRVEVMPNMKIRVDGRVVSIRESEQEICRVLAYYKPEGELC
TRRDPEGRPTVFDRLPRLRGSRWIAVGRLDINTSGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDEDKLRQLRRGV
QLEDGPASFKSISFQGGEGLNQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDLPLPKGLPRGGWQELPLENINYL
RELVELQAETASKLPVERERRRMKANQIRRAVKRHGQHSGGSRRAPTTKRG

Sequences:

>Translated_291_residues
MSEKLQKVLARAGHGSRREIEALIKEGRVSVDGKLATLGDRVEVMPNMKIRVDGRVVSIRESEQEICRVLAYYKPEGELC
TRRDPEGRPTVFDRLPRLRGSRWIAVGRLDINTSGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDEDKLRQLRRGV
QLEDGPASFKSISFQGGEGLNQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDLPLPKGLPRGGWQELPLENINYL
RELVELQAETASKLPVERERRRMKANQIRRAVKRHGQHSGGSRRAPTTKRG
>Mature_290_residues
SEKLQKVLARAGHGSRREIEALIKEGRVSVDGKLATLGDRVEVMPNMKIRVDGRVVSIRESEQEICRVLAYYKPEGELCT
RRDPEGRPTVFDRLPRLRGSRWIAVGRLDINTSGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDEDKLRQLRRGVQ
LEDGPASFKSISFQGGEGLNQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDLPLPKGLPRGGWQELPLENINYLR
ELVELQAETASKLPVERERRRMKANQIRRAVKRHGQHSGGSRRAPTTKRG

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=283, Percent_Identity=83.3922261484099, Blast_Score=487, Evalue=1e-139,
Organism=Escherichia coli, GI1790453, Length=289, Percent_Identity=29.4117647058824, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1788510, Length=219, Percent_Identity=31.5068493150685, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI87081838, Length=194, Percent_Identity=34.5360824742268, Blast_Score=89, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 33293; Mature: 33162

Theoretical pI: Translated: 10.95; Mature: 10.95

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKLQKVLARAGHGSRREIEALIKEGRVSVDGKLATLGDRVEVMPNMKIRVDGRVVSIR
CCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCEEEECCCCEEECCCCEEEECCEEEEEE
ESEQEICRVLAYYKPEGELCTRRDPEGRPTVFDRLPRLRGSRWIAVGRLDINTSGLLLFT
CCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEEECCCCEEEEE
TDGELANRLMHPSREVEREYAVRVFGQVDEDKLRQLRRGVQLEDGPASFKSISFQGGEGL
CCHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCHHEEECCCCCCC
NQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDLPLPKGLPRGGWQELPLENINYL
CCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHH
RELVELQAETASKLPVERERRRMKANQIRRAVKRHGQHSGGSRRAPTTKRG
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SEKLQKVLARAGHGSRREIEALIKEGRVSVDGKLATLGDRVEVMPNMKIRVDGRVVSIR
CHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCEEEECCCCEEECCCCEEEECCEEEEEE
ESEQEICRVLAYYKPEGELCTRRDPEGRPTVFDRLPRLRGSRWIAVGRLDINTSGLLLFT
CCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEEECCCCEEEEE
TDGELANRLMHPSREVEREYAVRVFGQVDEDKLRQLRRGVQLEDGPASFKSISFQGGEGL
CCHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCHHEEECCCCCCC
NQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDLPLPKGLPRGGWQELPLENINYL
CCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHH
RELVELQAETASKLPVERERRRMKANQIRRAVKRHGQHSGGSRRAPTTKRG
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]