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The map label for this gene is topB [H]

Identifier: 238919554

GI number: 238919554

Start: 1612525

End: 1614450

Strand: Direct

Name: topB [H]

Synonym: NT01EI_1650

Alternate gene names: 238919554

Gene position: 1612525-1614450 (Clockwise)

Preceding gene: 238919553

Following gene: 238919555

Centisome position: 42.3

GC content: 62.77

Gene sequence:

>1926_bases
ATGCGGCTGTTTATTGCGGAAAAGCCCAGCCTGGGGCGGGCTATTGCGGATGTGTTGCCTAAGCCGCACCGGCGTGCGGA
CGGCTATATTCAGTGCGGCGACGGTGACGTGGTGACCTGGTGTGTGGGCCATCTGCTGGAGCAGGCCGAGCCGGACGCCT
ACGATGCCCGCTACGCGCGCTGGAATCTGGTCGATCTGCCGATTGTACCGCAGAAGTGGCAGCTGCGACCCAAAGCATCG
AGCGCCAAGCAGCTGAAGGTGATCGAGCGCCTGCTGCGCGATGCGCAGCAGGTGGTGCACGCCGGCGACCCGGATCGCGA
AGGACAGCTGCTGGTGGATGAGGTGCTCGACTTTCTGGCCTTGGCGCCGGAAAAGCGGCGTCAGGTACAGCGTTGCCTGA
TCAATGACCTTAACCCCCAGGCGGTGGCGCGGGCGCTGGAGCGACTGCGCGATAACCATGAGTTTATGCCGCTGTGCGTC
TCTGCGCTGGCGCGCGCGCGCGCCGACTGGCTGTACGGCATCAACATGACCCGCGCCTATACCCTGCTGGGGCGCAACGC
CGGCTATAACGGCGTGCTCTCCGTGGGCCGGGTGCAGACGCCGGTGCTCGGGCTGGTGGTGCGCCGCGATGAGGAGATAG
CGCACTTTGTCGCCAAGGATTACTACGAGGTGCGGGCGCATATCGTGACGCCATCCGGCGAGCGCTTCTGGGCGCAGTGG
CAGCCCAGCGAGGCCTGTGAGCCCCATCAGGATGAAGAGGGGCGTCTGCTCAACCGCGCGCTGGCGGAGCATGTTATCGC
CCGGATCCAGGATAAGCCTGCCCGGGTGACCGGCTATAACGATAAGCGTGAGTCCGAGGTGGCGCCGCTGCCGTTCTCCC
TGTCAGCGCTGCAGATCGAGGCCGCCAAGCGCTTTGCCATGAGCGCTCAGCAGGTGCTGGATATCTGTCAACGTCTGTAT
GAAACCCACAAGCTATTGACCTATCCCCGCTCTGACAGCCGTCATCTGCCGGAGGAGCACTTTGCTGGGCGCCATGCGGT
GATGGCGGCGATTGCCACCCATGCGCCGACGCTGGCCAATCTGGAGAGCGTCGACGTCCAGCGGCGTAACCGCTGCTGGG
ATGACAACAAGGTTGATGCCCACCATGCCATCATTCCTACGGCCCGGGCAGGCGGCGTTACGCTGAATGAGGATGAGCGC
AATATTTATCAGCTGGTGGCCCGTCAGTATCTGATGCAGTTTTGCCCCGATGCGCAGTACCGTAAGTGTGTCATCGATCT
GGAGATTGAGGGCGGGGGATTTATCGCCAAGGCGCGATTTCTGTCTCAGGCCGGCTGGCGTGCGCTGCTCGGTGCCAAGG
AGCGGGATGACGAAGACGAGGGAATGCCGCTGCCGGTGGTGGCCCGCGATGACCTGCTGCACTGCGAACGGGGCGAGCTG
ATGGCGCGCCAGACGCAGCCGCCGCGCCACTTTACCGATGCTACACTGCTGTCGGCCATGACCGGGATCGCCCGCTTTGT
ACAGGATAAATCGCTAAAGAAAATCCTGCGAGCGACCGATGGTTTAGGTACCGAGGCGACGCGTGCCGGTATTATCGAAC
TGCTGTTTCGCCGCGGGTTTCTGCATAAAAAGGGGCGCTATATCCACGCCAGCCAGACGGGAAGTGCGCTGATCCACTGT
TTGCCCGAGATGGCCGGTCGCCCGGATATGACGGCACACTGGGAGTCGACGCTGACGAAGATCAGTGAGCGGGGCTGCCG
CTATCAGGACTTTATGCACCCGCTGGTGGAGACGCTGCAGCAGCTGATCCATCAGGCGCGCACTGCCGTGAACCCCCGCG
CGTTCCGTGATTTGCCGCCCATTGAAAACGGCCGGTCACGGCGCACCAAGGGGACAACTGCGTCCAATAAGAGGAAAAAA
GCATGA

Upstream 100 bases:

>100_bases
GCTACCAGTTGGAGAAGAAGGCAGCGCGGGTTGTTTTTCGCGGTGACTATCGGGCCGTCAGGGAATATCTCCTCTCTCTC
AGCCAGACATATGGTGACTG

Downstream 100 bases:

>100_bases
AGAAACAATCCGTAATGATGGCGTTTGTCGCGCTGCTGTGGAGCGGGGCGCTGCAGGCTCAGGGCACCGTCTCTACCAAA
GGTGCTCAGCAGGGGGCCAC

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 641; Mature: 641

Protein sequence:

>641_residues
MRLFIAEKPSLGRAIADVLPKPHRRADGYIQCGDGDVVTWCVGHLLEQAEPDAYDARYARWNLVDLPIVPQKWQLRPKAS
SAKQLKVIERLLRDAQQVVHAGDPDREGQLLVDEVLDFLALAPEKRRQVQRCLINDLNPQAVARALERLRDNHEFMPLCV
SALARARADWLYGINMTRAYTLLGRNAGYNGVLSVGRVQTPVLGLVVRRDEEIAHFVAKDYYEVRAHIVTPSGERFWAQW
QPSEACEPHQDEEGRLLNRALAEHVIARIQDKPARVTGYNDKRESEVAPLPFSLSALQIEAAKRFAMSAQQVLDICQRLY
ETHKLLTYPRSDSRHLPEEHFAGRHAVMAAIATHAPTLANLESVDVQRRNRCWDDNKVDAHHAIIPTARAGGVTLNEDER
NIYQLVARQYLMQFCPDAQYRKCVIDLEIEGGGFIAKARFLSQAGWRALLGAKERDDEDEGMPLPVVARDDLLHCERGEL
MARQTQPPRHFTDATLLSAMTGIARFVQDKSLKKILRATDGLGTEATRAGIIELLFRRGFLHKKGRYIHASQTGSALIHC
LPEMAGRPDMTAHWESTLTKISERGCRYQDFMHPLVETLQQLIHQARTAVNPRAFRDLPPIENGRSRRTKGTTASNKRKK
A

Sequences:

>Translated_641_residues
MRLFIAEKPSLGRAIADVLPKPHRRADGYIQCGDGDVVTWCVGHLLEQAEPDAYDARYARWNLVDLPIVPQKWQLRPKAS
SAKQLKVIERLLRDAQQVVHAGDPDREGQLLVDEVLDFLALAPEKRRQVQRCLINDLNPQAVARALERLRDNHEFMPLCV
SALARARADWLYGINMTRAYTLLGRNAGYNGVLSVGRVQTPVLGLVVRRDEEIAHFVAKDYYEVRAHIVTPSGERFWAQW
QPSEACEPHQDEEGRLLNRALAEHVIARIQDKPARVTGYNDKRESEVAPLPFSLSALQIEAAKRFAMSAQQVLDICQRLY
ETHKLLTYPRSDSRHLPEEHFAGRHAVMAAIATHAPTLANLESVDVQRRNRCWDDNKVDAHHAIIPTARAGGVTLNEDER
NIYQLVARQYLMQFCPDAQYRKCVIDLEIEGGGFIAKARFLSQAGWRALLGAKERDDEDEGMPLPVVARDDLLHCERGEL
MARQTQPPRHFTDATLLSAMTGIARFVQDKSLKKILRATDGLGTEATRAGIIELLFRRGFLHKKGRYIHASQTGSALIHC
LPEMAGRPDMTAHWESTLTKISERGCRYQDFMHPLVETLQQLIHQARTAVNPRAFRDLPPIENGRSRRTKGTTASNKRKK
A
>Mature_641_residues
MRLFIAEKPSLGRAIADVLPKPHRRADGYIQCGDGDVVTWCVGHLLEQAEPDAYDARYARWNLVDLPIVPQKWQLRPKAS
SAKQLKVIERLLRDAQQVVHAGDPDREGQLLVDEVLDFLALAPEKRRQVQRCLINDLNPQAVARALERLRDNHEFMPLCV
SALARARADWLYGINMTRAYTLLGRNAGYNGVLSVGRVQTPVLGLVVRRDEEIAHFVAKDYYEVRAHIVTPSGERFWAQW
QPSEACEPHQDEEGRLLNRALAEHVIARIQDKPARVTGYNDKRESEVAPLPFSLSALQIEAAKRFAMSAQQVLDICQRLY
ETHKLLTYPRSDSRHLPEEHFAGRHAVMAAIATHAPTLANLESVDVQRRNRCWDDNKVDAHHAIIPTARAGGVTLNEDER
NIYQLVARQYLMQFCPDAQYRKCVIDLEIEGGGFIAKARFLSQAGWRALLGAKERDDEDEGMPLPVVARDDLLHCERGEL
MARQTQPPRHFTDATLLSAMTGIARFVQDKSLKKILRATDGLGTEATRAGIIELLFRRGFLHKKGRYIHASQTGSALIHC
LPEMAGRPDMTAHWESTLTKISERGCRYQDFMHPLVETLQQLIHQARTAVNPRAFRDLPPIENGRSRRTKGTTASNKRKK
A

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=584, Percent_Identity=22.9452054794521, Blast_Score=117, Evalue=5e-26,
Organism=Homo sapiens, GI10835218, Length=612, Percent_Identity=23.6928104575163, Blast_Score=93, Evalue=9e-19,
Organism=Escherichia coli, GI1788061, Length=643, Percent_Identity=75.5832037325039, Blast_Score=1012, Evalue=0.0,
Organism=Escherichia coli, GI1787529, Length=531, Percent_Identity=25.6120527306968, Blast_Score=114, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI32563869, Length=619, Percent_Identity=21.8093699515347, Blast_Score=95, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6323263, Length=694, Percent_Identity=23.342939481268, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24640096, Length=632, Percent_Identity=22.1518987341772, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24585251, Length=533, Percent_Identity=24.953095684803, Blast_Score=89, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 72520; Mature: 72520

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLFIAEKPSLGRAIADVLPKPHRRADGYIQCGDGDVVTWCVGHLLEQAEPDAYDARYAR
CEEEEECCCCHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCHHCE
WNLVDLPIVPQKWQLRPKASSAKQLKVIERLLRDAQQVVHAGDPDREGQLLVDEVLDFLA
EEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
LAPEKRRQVQRCLINDLNPQAVARALERLRDNHEFMPLCVSALARARADWLYGINMTRAY
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHH
TLLGRNAGYNGVLSVGRVQTPVLGLVVRRDEEIAHFVAKDYYEVRAHIVTPSGERFWAQW
HHHCCCCCCCCCEECCCHHCCHHHHHEECCHHHHHHHHHHHHHEEEEEECCCCCCEEECC
QPSEACEPHQDEEGRLLNRALAEHVIARIQDKPARVTGYNDKRESEVAPLPFSLSALQIE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCHHHHHHH
AAKRFAMSAQQVLDICQRLYETHKLLTYPRSDSRHLPEEHFAGRHAVMAAIATHAPTLAN
HHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHCCHHHHHHHHHHCCCCHHC
LESVDVQRRNRCWDDNKVDAHHAIIPTARAGGVTLNEDERNIYQLVARQYLMQFCPDAQY
CHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCEECCCHHHHHHHHHHHHHHHHCCCCCC
RKCVIDLEIEGGGFIAKARFLSQAGWRALLGAKERDDEDEGMPLPVVARDDLLHCERGEL
CEEEEEEEECCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCCH
MARQTQPPRHFTDATLLSAMTGIARFVQDKSLKKILRATDGLGTEATRAGIIELLFRRGF
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
LHKKGRYIHASQTGSALIHCLPEMAGRPDMTAHWESTLTKISERGCRYQDFMHPLVETLQ
HHCCCCEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
QLIHQARTAVNPRAFRDLPPIENGRSRRTKGTTASNKRKKA
HHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRLFIAEKPSLGRAIADVLPKPHRRADGYIQCGDGDVVTWCVGHLLEQAEPDAYDARYAR
CEEEEECCCCHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCHHCE
WNLVDLPIVPQKWQLRPKASSAKQLKVIERLLRDAQQVVHAGDPDREGQLLVDEVLDFLA
EEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
LAPEKRRQVQRCLINDLNPQAVARALERLRDNHEFMPLCVSALARARADWLYGINMTRAY
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHH
TLLGRNAGYNGVLSVGRVQTPVLGLVVRRDEEIAHFVAKDYYEVRAHIVTPSGERFWAQW
HHHCCCCCCCCCEECCCHHCCHHHHHEECCHHHHHHHHHHHHHEEEEEECCCCCCEEECC
QPSEACEPHQDEEGRLLNRALAEHVIARIQDKPARVTGYNDKRESEVAPLPFSLSALQIE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCHHHHHHH
AAKRFAMSAQQVLDICQRLYETHKLLTYPRSDSRHLPEEHFAGRHAVMAAIATHAPTLAN
HHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHCCHHHHHHHHHHCCCCHHC
LESVDVQRRNRCWDDNKVDAHHAIIPTARAGGVTLNEDERNIYQLVARQYLMQFCPDAQY
CHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCEECCCHHHHHHHHHHHHHHHHCCCCCC
RKCVIDLEIEGGGFIAKARFLSQAGWRALLGAKERDDEDEGMPLPVVARDDLLHCERGEL
CEEEEEEEECCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCCH
MARQTQPPRHFTDATLLSAMTGIARFVQDKSLKKILRATDGLGTEATRAGIIELLFRRGF
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
LHKKGRYIHASQTGSALIHCLPEMAGRPDMTAHWESTLTKISERGCRYQDFMHPLVETLQ
HHCCCCEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
QLIHQARTAVNPRAFRDLPPIENGRSRRTKGTTASNKRKKA
HHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2553698; 9097039; 9278503; 10574789 [H]