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The map label for this gene is sppA [H]

Identifier: 238919550

GI number: 238919550

Start: 1607546

End: 1609399

Strand: Reverse

Name: sppA [H]

Synonym: NT01EI_1646

Alternate gene names: 238919550

Gene position: 1609399-1607546 (Counterclockwise)

Preceding gene: 238919556

Following gene: 238919549

Centisome position: 42.22

GC content: 59.6

Gene sequence:

>1854_bases
ATGCGCATGCTATGGCGCTATATCGCCGGCGCGTTTCGCTGCTGCTGGCAAATACTCAATTTTATTCGTGAGTTTATCCT
TAATCTGTTTTTGATCCTGCTGATCCTGATCGGCGTCGGTATCTACCTTCAGTTTCAGGATACGCCCGTCAATCCCACGA
CCAAAGGTGCTCTGCTGGTGGATCTCAACGGCGTGGTGGTCGATAACCCCGCGCTGAACAGCAAATTCGGACAGCTGGGC
CGAGAGCTGCTGGGCATCCGCGGCGCAGCGCTACAGGAGAACTCCCTGTTCGATCTGGTGGATACCCTGCGCCAGGCCAA
AAGCGATCCCAATATTACCGGGCTGGTTTTATCGCTGAATAACTTTGCCGGTGCCGATCAGCCGTCACTGCAGTACATTG
GCAAGGCGCTGAGCGAGTTCCGTGCCAGCGGTAAGCCGATCCTGGCAGTAGGTGAAAACTACACTCAGGCGCAGTATTTC
CTGGCCAGCTATGCGGATAAGATCTACCTCTCTCCCCAGGGCAGCGTCGGGCTGCAGGGAATGGCGACCAATAACCTCTA
CTATAAGAGCCTGCTGGATAAACTGGATGTCAGCACCCATATCTTCCGCGTCGGCACCTATAAGTCCGCCGTCGAACCGC
TGATGCGCGACCAGATGTCACCGCAGGCGCGCAGCGCTGACAGCCAATGGCTCAACGGTATGTGGAGTAACTATCTGACC
ATCGTTGCCACCAACCGGCACCTTACGCCGGAGCAGGTTTTCCCCGGCGCCGGGCAGATGCTGAAAGGGCTACAGGCCAC
CGGCGGTGATATGGCGCAGTACGCCGTACTACACCGGCTGGTCGATGGGCTGGCATCGCGCAGCGAGGCCGAAAGCATTC
TGTCCAGGCAGTTTGGCTGGGATGCACGCGCGCGCGATTACCGCCATATCAGCATCTATGACTATCCCCTGAAACCGAAG
CGTACTGGTAATGGTCAAATCGCCGTTATTTTTGCCAGCGGCCCGATCATCGATGGTGAGGCGACGCCGGGCAGCGTAGG
TGGTGATACCACCGCAGCCCAGATCCGCCAGGCGCGTCTCGACCCGGCGATCCGGGCGATAATCCTGCGGGTCAACAGCC
CCGGCGGCAGTGTCACCGCCTCTGAAGAGATCCGCTCGGCGCTGGCGGCAGCGCGCGACGCCGGTAAACCGGTGGTGGTC
TCCATGGGGGGGCTGGCAGCATCCGGCGGTTACTGGATCTCGACACCTGCCAACACCATTGTGGCCAGCCCCAGCACCCT
TACCGGCTCTATCGGGATCTTTGGGGTCATCAATACCTTTGAAAAGCCGCTGGGACTGCTGGGGGTCTATACGGACGGCG
TCGCGACCTCGCCTCTGGCCGATACCGCCCTGACCAAAGCGCTGCCGCAGAGCGTCAGCGATCTGATGCAGCTGACTATC
GAAAACGGCTATCAGCGCTTTATCAGTCTGGTGGCCACCGCCCGTCATAAAACGCCGGCGCAGATCGATAACATTGCGCA
GGGGCGTGTCTGGCTAGGCGTAGATGCCAAAGCCAATGGGCTGGTCGATCGATTGGGCGACTTTGACGATGCTGTTAGCC
AGGCGGCGACGCTGGCCGGTATCAAGCAGCCCCAGCTGCAGTGGTATGTCGATAATCCGAGCTTCAGTGAAATGGTGTTC
AGCCAGCTGACCAGCTCCGTACAAGCCTCTCTGCCGCAGGCGCTGCAGGGCATGATCCCCGCCTCACTGCTCCAGCTCTC
GCGCCAGATTTCGGCGCAGAGCGATTGGGTCGCTTCCCTCAATGATCCGCAGCAGCGCTACGCGCTGTGCCTGAGCTGTA
GCGAGATCCGTTAA

Upstream 100 bases:

>100_bases
CACAGAGGGCGGACCAGCGCTGCCGGAGGTATATCAACGCTGACTTTTTCCGTCGACGCCGCTAGGATAGCGGGATTGCA
ATCATTGACCTGGAGTTTAC

Downstream 100 bases:

>100_bases
TCCTGCCACCCGCCGGAGCAGCAGCCCATTATTCATGGCTGTTGGCCCGGCGGTCTTTTTTTCTTTTGTATTATCCCTAT
AATCCCCGCATATTTGCGCA

Product: signal peptide peptidase SppA, 67K type

Products: NA

Alternate protein names: Endopeptidase IV; Protease IV; Signal peptide peptidase [H]

Number of amino acids: Translated: 617; Mature: 617

Protein sequence:

>617_residues
MRMLWRYIAGAFRCCWQILNFIREFILNLFLILLILIGVGIYLQFQDTPVNPTTKGALLVDLNGVVVDNPALNSKFGQLG
RELLGIRGAALQENSLFDLVDTLRQAKSDPNITGLVLSLNNFAGADQPSLQYIGKALSEFRASGKPILAVGENYTQAQYF
LASYADKIYLSPQGSVGLQGMATNNLYYKSLLDKLDVSTHIFRVGTYKSAVEPLMRDQMSPQARSADSQWLNGMWSNYLT
IVATNRHLTPEQVFPGAGQMLKGLQATGGDMAQYAVLHRLVDGLASRSEAESILSRQFGWDARARDYRHISIYDYPLKPK
RTGNGQIAVIFASGPIIDGEATPGSVGGDTTAAQIRQARLDPAIRAIILRVNSPGGSVTASEEIRSALAAARDAGKPVVV
SMGGLAASGGYWISTPANTIVASPSTLTGSIGIFGVINTFEKPLGLLGVYTDGVATSPLADTALTKALPQSVSDLMQLTI
ENGYQRFISLVATARHKTPAQIDNIAQGRVWLGVDAKANGLVDRLGDFDDAVSQAATLAGIKQPQLQWYVDNPSFSEMVF
SQLTSSVQASLPQALQGMIPASLLQLSRQISAQSDWVASLNDPQQRYALCLSCSEIR

Sequences:

>Translated_617_residues
MRMLWRYIAGAFRCCWQILNFIREFILNLFLILLILIGVGIYLQFQDTPVNPTTKGALLVDLNGVVVDNPALNSKFGQLG
RELLGIRGAALQENSLFDLVDTLRQAKSDPNITGLVLSLNNFAGADQPSLQYIGKALSEFRASGKPILAVGENYTQAQYF
LASYADKIYLSPQGSVGLQGMATNNLYYKSLLDKLDVSTHIFRVGTYKSAVEPLMRDQMSPQARSADSQWLNGMWSNYLT
IVATNRHLTPEQVFPGAGQMLKGLQATGGDMAQYAVLHRLVDGLASRSEAESILSRQFGWDARARDYRHISIYDYPLKPK
RTGNGQIAVIFASGPIIDGEATPGSVGGDTTAAQIRQARLDPAIRAIILRVNSPGGSVTASEEIRSALAAARDAGKPVVV
SMGGLAASGGYWISTPANTIVASPSTLTGSIGIFGVINTFEKPLGLLGVYTDGVATSPLADTALTKALPQSVSDLMQLTI
ENGYQRFISLVATARHKTPAQIDNIAQGRVWLGVDAKANGLVDRLGDFDDAVSQAATLAGIKQPQLQWYVDNPSFSEMVF
SQLTSSVQASLPQALQGMIPASLLQLSRQISAQSDWVASLNDPQQRYALCLSCSEIR
>Mature_617_residues
MRMLWRYIAGAFRCCWQILNFIREFILNLFLILLILIGVGIYLQFQDTPVNPTTKGALLVDLNGVVVDNPALNSKFGQLG
RELLGIRGAALQENSLFDLVDTLRQAKSDPNITGLVLSLNNFAGADQPSLQYIGKALSEFRASGKPILAVGENYTQAQYF
LASYADKIYLSPQGSVGLQGMATNNLYYKSLLDKLDVSTHIFRVGTYKSAVEPLMRDQMSPQARSADSQWLNGMWSNYLT
IVATNRHLTPEQVFPGAGQMLKGLQATGGDMAQYAVLHRLVDGLASRSEAESILSRQFGWDARARDYRHISIYDYPLKPK
RTGNGQIAVIFASGPIIDGEATPGSVGGDTTAAQIRQARLDPAIRAIILRVNSPGGSVTASEEIRSALAAARDAGKPVVV
SMGGLAASGGYWISTPANTIVASPSTLTGSIGIFGVINTFEKPLGLLGVYTDGVATSPLADTALTKALPQSVSDLMQLTI
ENGYQRFISLVATARHKTPAQIDNIAQGRVWLGVDAKANGLVDRLGDFDDAVSQAATLAGIKQPQLQWYVDNPSFSEMVF
SQLTSSVQASLPQALQGMIPASLLQLSRQISAQSDWVASLNDPQQRYALCLSCSEIR

Specific function: Digestion of the cleaved signal peptides. This activity is necessary to maintain proper secretion of mature proteins across the membrane [H]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=619, Percent_Identity=63.4894991922456, Blast_Score=805, Evalue=0.0,
Organism=Escherichia coli, GI1787527, Length=170, Percent_Identity=28.8235294117647, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004634
- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.-

Molecular weight: Translated: 66585; Mature: 66585

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMLWRYIAGAFRCCWQILNFIREFILNLFLILLILIGVGIYLQFQDTPVNPTTKGALLV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEE
DLNGVVVDNPALNSKFGQLGRELLGIRGAALQENSLFDLVDTLRQAKSDPNITGLVLSLN
EECCEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEC
NFAGADQPSLQYIGKALSEFRASGKPILAVGENYTQAQYFLASYADKIYLSPQGSVGLQG
CCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCEEEECCCCCCCCCE
MATNNLYYKSLLDKLDVSTHIFRVGTYKSAVEPLMRDQMSPQARSADSQWLNGMWSNYLT
EECCCHHHHHHHHHHCCHHHEEEECCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCEEE
IVATNRHLTPEQVFPGAGQMLKGLQATGGDMAQYAVLHRLVDGLASRSEAESILSRQFGW
EEEECCCCCHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCC
DARARDYRHISIYDYPLKPKRTGNGQIAVIFASGPIIDGEATPGSVGGDTTAAQIRQARL
CCCCCCCEEEEEEECCCCCCCCCCCEEEEEEECCCEECCCCCCCCCCCCHHHHHHHHHHC
DPAIRAIILRVNSPGGSVTASEEIRSALAAARDAGKPVVVSMGGLAASGGYWISTPANTI
CCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCEEECCCEEEECCCCEE
VASPSTLTGSIGIFGVINTFEKPLGLLGVYTDGVATSPLADTALTKALPQSVSDLMQLTI
EECCCCCCCCCHHHHHHHHHHCCHHEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ENGYQRFISLVATARHKTPAQIDNIAQGRVWLGVDAKANGLVDRLGDFDDAVSQAATLAG
HHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEEECCCCCHHHHHHCCHHHHHHHHHHHHC
IKQPQLQWYVDNPSFSEMVFSQLTSSVQASLPQALQGMIPASLLQLSRQISAQSDWVASL
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCC
NDPQQRYALCLSCSEIR
CCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRMLWRYIAGAFRCCWQILNFIREFILNLFLILLILIGVGIYLQFQDTPVNPTTKGALLV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEE
DLNGVVVDNPALNSKFGQLGRELLGIRGAALQENSLFDLVDTLRQAKSDPNITGLVLSLN
EECCEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEC
NFAGADQPSLQYIGKALSEFRASGKPILAVGENYTQAQYFLASYADKIYLSPQGSVGLQG
CCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCEEEECCCCCCCCCE
MATNNLYYKSLLDKLDVSTHIFRVGTYKSAVEPLMRDQMSPQARSADSQWLNGMWSNYLT
EECCCHHHHHHHHHHCCHHHEEEECCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCEEE
IVATNRHLTPEQVFPGAGQMLKGLQATGGDMAQYAVLHRLVDGLASRSEAESILSRQFGW
EEEECCCCCHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCC
DARARDYRHISIYDYPLKPKRTGNGQIAVIFASGPIIDGEATPGSVGGDTTAAQIRQARL
CCCCCCCEEEEEEECCCCCCCCCCCEEEEEEECCCEECCCCCCCCCCCCHHHHHHHHHHC
DPAIRAIILRVNSPGGSVTASEEIRSALAAARDAGKPVVVSMGGLAASGGYWISTPANTI
CCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCEEECCCEEEECCCCEE
VASPSTLTGSIGIFGVINTFEKPLGLLGVYTDGVATSPLADTALTKALPQSVSDLMQLTI
EECCCCCCCCCHHHHHHHHHHCCHHEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ENGYQRFISLVATARHKTPAQIDNIAQGRVWLGVDAKANGLVDRLGDFDDAVSQAATLAG
HHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEEECCCCCHHHHHHCCHHHHHHHHHHHHC
IKQPQLQWYVDNPSFSEMVFSQLTSSVQASLPQALQGMIPASLLQLSRQISAQSDWVASL
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCC
NDPQQRYALCLSCSEIR
CCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]