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The map label for this gene is nudB [H]

Identifier: 238919499

GI number: 238919499

Start: 1557567

End: 1558001

Strand: Direct

Name: nudB [H]

Synonym: NT01EI_1595

Alternate gene names: 238919499

Gene position: 1557567-1558001 (Clockwise)

Preceding gene: 238919498

Following gene: 238919500

Centisome position: 40.86

GC content: 61.38

Gene sequence:

>435_bases
ATGGCCTATAAGCGTCCGGAATCCATCCTGGTGGTGATCTATGCCCGCCAGGGGGGACGGGTGCTGATGTTGCAGCGGCG
CGATGACCCCGATTTCTGGCAGTCGGTAACCGGCAGTCTGGAATCGGGAGAGTCGCCGTCGCAGGCTGCGCAGCGTGAAG
TAAAGGAAGAAGTGGGTATCGACATCGTCAAGGAGCATTTGACGCTGACGGACTGCCAGCGCTGCGTGGAGTTTGAACTG
TTTGCGCACCTGCGCCACCGCTATGCGCCCGGCGTGACGCGTAACCTAGAGCATTGGTTCTGTTTGGCCATCCCTGCTGA
GCGGGCGATCCCGCTCAGCGAACATCTGGCCTACCGTTGGCTGGACGCCGCAGGGGCGGCCGGGCTGACCAAATCCTGGA
GTAACCGACAGGCGATCGAACAGTTCGTCCCTTGA

Upstream 100 bases:

>100_bases
CGGCGGCCTGTCCGCTGACCGACGCCCCGAGCCGCGCCAACCCGGCGGCGCTGCAGGAGCTCTCTATCGCCGTCTGTGCC
AAACAGGGCAGCGACGCGTA

Downstream 100 bases:

>100_bases
TTACGTGCCTGTACGGGCGGTCATACTGGCCGTTCGCATTGATTTACAGGCGTGCATATTGACTATTTTAGGGCCTTTGC
GCCTTTAGCGGAGATTTATA

Product: hypothetical protein

Products: dAMP; pyrophosphate [C]

Alternate protein names: dATP pyrophosphohydrolase [H]

Number of amino acids: Translated: 144; Mature: 143

Protein sequence:

>144_residues
MAYKRPESILVVIYARQGGRVLMLQRRDDPDFWQSVTGSLESGESPSQAAQREVKEEVGIDIVKEHLTLTDCQRCVEFEL
FAHLRHRYAPGVTRNLEHWFCLAIPAERAIPLSEHLAYRWLDAAGAAGLTKSWSNRQAIEQFVP

Sequences:

>Translated_144_residues
MAYKRPESILVVIYARQGGRVLMLQRRDDPDFWQSVTGSLESGESPSQAAQREVKEEVGIDIVKEHLTLTDCQRCVEFEL
FAHLRHRYAPGVTRNLEHWFCLAIPAERAIPLSEHLAYRWLDAAGAAGLTKSWSNRQAIEQFVP
>Mature_143_residues
AYKRPESILVVIYARQGGRVLMLQRRDDPDFWQSVTGSLESGESPSQAAQREVKEEVGIDIVKEHLTLTDCQRCVEFELF
AHLRHRYAPGVTRNLEHWFCLAIPAERAIPLSEHLAYRWLDAAGAAGLTKSWSNRQAIEQFVP

Specific function: Catalyzes the hydrolysis of dihydroneopterin triphosphate to dihydroneopterin monophosphate and pyrophosphate. Required for efficient folate biosynthesis. Can also hydrolyze nucleoside triphosphates with a preference for dATP [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1788172, Length=141, Percent_Identity=78.0141843971631, Blast_Score=234, Evalue=2e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003564
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.1.- [C]

Molecular weight: Translated: 16434; Mature: 16303

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00362 RIBOSOMAL_S15 ; PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYKRPESILVVIYARQGGRVLMLQRRDDPDFWQSVTGSLESGESPSQAAQREVKEEVGI
CCCCCCCCEEEEEEECCCCEEEEEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCH
DIVKEHLTLTDCQRCVEFELFAHLRHRYAPGVTRNLEHWFCLAIPAERAIPLSEHLAYRW
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHH
LDAAGAAGLTKSWSNRQAIEQFVP
HHHCCCCCCCCCCCCHHHHHHHCC
>Mature Secondary Structure 
AYKRPESILVVIYARQGGRVLMLQRRDDPDFWQSVTGSLESGESPSQAAQREVKEEVGI
CCCCCCCEEEEEEECCCCEEEEEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCH
DIVKEHLTLTDCQRCVEFELFAHLRHRYAPGVTRNLEHWFCLAIPAERAIPLSEHLAYRW
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHH
LDAAGAAGLTKSWSNRQAIEQFVP
HHHCCCCCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxy-ATP; H2O [C]

Specific reaction: deoxy-ATP + H2O = dAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]