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The map label for this gene is aspS

Identifier: 238919498

GI number: 238919498

Start: 1555792

End: 1557567

Strand: Direct

Name: aspS

Synonym: NT01EI_1594

Alternate gene names: 238919498

Gene position: 1555792-1557567 (Clockwise)

Preceding gene: 238919497

Following gene: 238919499

Centisome position: 40.81

GC content: 61.82

Gene sequence:

>1776_bases
ATGCGTACTAATTATTGCGGGCAGTTGAATCTGTCCCATGTGGGCCAGGAAGTGACCCTGTGTGGTTGGGTACACCGCCG
CCGTGATTTGGGCGGTCTGATCTTTATCGATCTGCGCGACCGCGAAGGGGTGGTTCAGGTGTTCTTCGATCCGGATCATC
AGGATGCCTTCCGTCAGGCCTCTGAGCTGCGTAACGAGTTTTGCGTGCAGGTAACCGGTACCGTGCGTGCGCGCCCGGAG
AGTCAGCGTAACAGCGAGATGCCGACCGGGGAGATCGAAGTCTTTGGCCATGGCCTGACCCTCATCAACCGCGCCGAGCC
GCTGCCGCTGGACTTTAACCAGACTAACAGTGAAGAGAATCGCCTCAAGTACCGCTACCTGGATCTGCGTCGTCCGGAGA
TGGCTGCGCGTCTGAAGACCCGCGCCAAGATCACCGCATTCGTGCGTCGTTTCATGGATAACCACGGCTTCCTGGATATC
GAAACCCCGATGCTGACCAAGGCGACGCCGGAAGGGGCGCGTGACTACCTGGTGCCGAGCCGGGTGCATAAAGGCAAGTT
TTATGCGTTGCCGCAGTCGCCCCAGCTGTTTAAACAGCTGCTGATGATGTCCGGCTTTGACCGCTACTACCAGATAGTGA
AGTGCTTCCGCGACGAAGATCTGCGCGCAGACCGTCAGCCGGAGTTCACCCAGATCGATGTGGAGACCTCTTTCATGAGC
GCCGGGCAGGTGCGTGAGATCATGGAAGCGCTGGCCCGTGCGCTGTGGATGGAGATCAAAGGGGTGGATCTGGGCGACTT
CCCGGTGATGACCTTTGCAGAGGCGATGCGCCGTTTCGGCTCGGATAAGCCGGATCTGCGTAACCCGCTGGAGCTGGTCG
ATGTGGCCGATCTGGTTAAGAGCGTCGACTTTAAGGTCTTCTCCGGTCCGGCCAACGATGCCAGAGGGCGGGTGATCGCG
CTGCGCGTGCCGGGCGGTGCGACCCTGACCCGTAAGAATATCGATGAGTACGGTCAGTTTGTCGGCATCTATGGCGCCAA
AGGGCTCGCCTGGATGAAGGTCAACGATCGTGCCGCCGGGATGGATGGCGTGCAGAGCCCGATCGCTAAGTTCCTCAACG
CCGAGGTGCTGGAAGGCATTCTGGCGCGCAGCGGCGCGCAGAGCGGCGACATCATCTTCTTCGGCGCCGACAGCGCCAAG
GTAGCCACCGATGCCATGGGTGCGCTGCGTCTGAAGGTCGGGCGCGATCTGCAGCTGACCGACGAGTCACGCTGGGCGCC
GCTGTGGGTGGTCGATTTCCCGATGTTCGAAGAGGACGGCGAAGGCGGCCTGGCCGCGATGCACCACCCGTTCACCTCGC
CGCGCGATATCAGCCCGGAGGCGCTGAAGGCCAATCCGGTCGGCGCCATCGCCAACGCCTACGATATGGTGATGAATGGC
TATGAGGTGGGCGGTGGCTCCGTCCGTATTCACAGTGGTGCCATGCAGTCTGCGGTCTTCGATATTCTGGGGATCAACGA
GCAGGAGCAGCGCGAGAAGTTCGGCTTCCTGCTGGATGCGCTGAAGTTTGGCACCCCGCCGCACGCCGGTCTGGCGTTTG
GCCTGGATCGCCTGGTGATGCTGCTGACCGGTACGGAGAATATCCGCGACGTGATCGCATTCCCGAAAACCACGGCGGCG
GCCTGTCCGCTGACCGACGCCCCGAGCCGCGCCAACCCGGCGGCGCTGCAGGAGCTCTCTATCGCCGTCTGTGCCAAACA
GGGCAGCGACGCGTAA

Upstream 100 bases:

>100_bases
ACACCGTCTTCAACAGAGCGCTGCGCAGGCAGCCCGTGTCCGGCCGGTTGTCCGGGCGCGGCGTCGGCAAAATAAATTAA
GAAAGCCAAAGGATATCGTT

Downstream 100 bases:

>100_bases
TGGCCTATAAGCGTCCGGAATCCATCCTGGTGGTGATCTATGCCCGCCAGGGGGGACGGGTGCTGATGTTGCAGCGGCGC
GATGACCCCGATTTCTGGCA

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 591; Mature: 591

Protein sequence:

>591_residues
MRTNYCGQLNLSHVGQEVTLCGWVHRRRDLGGLIFIDLRDREGVVQVFFDPDHQDAFRQASELRNEFCVQVTGTVRARPE
SQRNSEMPTGEIEVFGHGLTLINRAEPLPLDFNQTNSEENRLKYRYLDLRRPEMAARLKTRAKITAFVRRFMDNHGFLDI
ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDVETSFMS
AGQVREIMEALARALWMEIKGVDLGDFPVMTFAEAMRRFGSDKPDLRNPLELVDVADLVKSVDFKVFSGPANDARGRVIA
LRVPGGATLTRKNIDEYGQFVGIYGAKGLAWMKVNDRAAGMDGVQSPIAKFLNAEVLEGILARSGAQSGDIIFFGADSAK
VATDAMGALRLKVGRDLQLTDESRWAPLWVVDFPMFEEDGEGGLAAMHHPFTSPRDISPEALKANPVGAIANAYDMVMNG
YEVGGGSVRIHSGAMQSAVFDILGINEQEQREKFGFLLDALKFGTPPHAGLAFGLDRLVMLLTGTENIRDVIAFPKTTAA
ACPLTDAPSRANPAALQELSIAVCAKQGSDA

Sequences:

>Translated_591_residues
MRTNYCGQLNLSHVGQEVTLCGWVHRRRDLGGLIFIDLRDREGVVQVFFDPDHQDAFRQASELRNEFCVQVTGTVRARPE
SQRNSEMPTGEIEVFGHGLTLINRAEPLPLDFNQTNSEENRLKYRYLDLRRPEMAARLKTRAKITAFVRRFMDNHGFLDI
ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDVETSFMS
AGQVREIMEALARALWMEIKGVDLGDFPVMTFAEAMRRFGSDKPDLRNPLELVDVADLVKSVDFKVFSGPANDARGRVIA
LRVPGGATLTRKNIDEYGQFVGIYGAKGLAWMKVNDRAAGMDGVQSPIAKFLNAEVLEGILARSGAQSGDIIFFGADSAK
VATDAMGALRLKVGRDLQLTDESRWAPLWVVDFPMFEEDGEGGLAAMHHPFTSPRDISPEALKANPVGAIANAYDMVMNG
YEVGGGSVRIHSGAMQSAVFDILGINEQEQREKFGFLLDALKFGTPPHAGLAFGLDRLVMLLTGTENIRDVIAFPKTTAA
ACPLTDAPSRANPAALQELSIAVCAKQGSDA
>Mature_591_residues
MRTNYCGQLNLSHVGQEVTLCGWVHRRRDLGGLIFIDLRDREGVVQVFFDPDHQDAFRQASELRNEFCVQVTGTVRARPE
SQRNSEMPTGEIEVFGHGLTLINRAEPLPLDFNQTNSEENRLKYRYLDLRRPEMAARLKTRAKITAFVRRFMDNHGFLDI
ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDVETSFMS
AGQVREIMEALARALWMEIKGVDLGDFPVMTFAEAMRRFGSDKPDLRNPLELVDVADLVKSVDFKVFSGPANDARGRVIA
LRVPGGATLTRKNIDEYGQFVGIYGAKGLAWMKVNDRAAGMDGVQSPIAKFLNAEVLEGILARSGAQSGDIIFFGADSAK
VATDAMGALRLKVGRDLQLTDESRWAPLWVVDFPMFEEDGEGGLAAMHHPFTSPRDISPEALKANPVGAIANAYDMVMNG
YEVGGGSVRIHSGAMQSAVFDILGINEQEQREKFGFLLDALKFGTPPHAGLAFGLDRLVMLLTGTENIRDVIAFPKTTAA
ACPLTDAPSRANPAALQELSIAVCAKQGSDA

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=604, Percent_Identity=39.9006622516556, Blast_Score=426, Evalue=1e-119,
Organism=Homo sapiens, GI45439306, Length=273, Percent_Identity=27.1062271062271, Blast_Score=89, Evalue=9e-18,
Organism=Homo sapiens, GI5031815, Length=249, Percent_Identity=26.9076305220884, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI194272210, Length=249, Percent_Identity=26.9076305220884, Blast_Score=86, Evalue=9e-17,
Organism=Homo sapiens, GI226491502, Length=257, Percent_Identity=24.9027237354086, Blast_Score=74, Evalue=5e-13,
Organism=Escherichia coli, GI1788173, Length=586, Percent_Identity=80.3754266211604, Blast_Score=999, Evalue=0.0,
Organism=Escherichia coli, GI1789256, Length=307, Percent_Identity=27.6872964169381, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1790571, Length=301, Percent_Identity=28.2392026578073, Blast_Score=99, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI32566633, Length=613, Percent_Identity=35.0734094616639, Blast_Score=317, Evalue=1e-86,
Organism=Caenorhabditis elegans, GI71994340, Length=256, Percent_Identity=25.78125, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17535925, Length=256, Percent_Identity=25.78125, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17535927, Length=256, Percent_Identity=25.78125, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17551876, Length=278, Percent_Identity=28.0575539568345, Blast_Score=86, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6325153, Length=649, Percent_Identity=33.2819722650231, Blast_Score=285, Evalue=2e-77,
Organism=Saccharomyces cerevisiae, GI6320242, Length=256, Percent_Identity=26.953125, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323011, Length=346, Percent_Identity=26.0115606936416, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6321807, Length=251, Percent_Identity=28.2868525896414, Blast_Score=78, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24584738, Length=593, Percent_Identity=35.9190556492412, Blast_Score=329, Evalue=4e-90,
Organism=Drosophila melanogaster, GI24640849, Length=255, Percent_Identity=29.4117647058824, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24640851, Length=255, Percent_Identity=29.4117647058824, Blast_Score=86, Evalue=9e-17,
Organism=Drosophila melanogaster, GI17136276, Length=259, Percent_Identity=25.8687258687259, Blast_Score=78, Evalue=2e-14,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_EDWI9 (C5B9S7)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002933013.1
- GeneID:   7961922
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_1594
- OMA:   AFPKTQQ
- ProtClustDB:   CLSK2813092
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 65507; Mature: 65507

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTNYCGQLNLSHVGQEVTLCGWVHRRRDLGGLIFIDLRDREGVVQVFFDPDHQDAFRQA
CCCCCCCCCCHHHCCCHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEECCCHHHHHHHH
SELRNEFCVQVTGTVRARPESQRNSEMPTGEIEVFGHGLTLINRAEPLPLDFNQTNSEEN
HHHHHHEEEEEEEEEECCCCHHCCCCCCCCCEEEEECCEEEEECCCCCCCCCCCCCCHHH
RLKYRYLDLRRPEMAARLKTRAKITAFVRRFMDNHGFLDIETPMLTKATPEGARDYLVPS
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEECCCCCCCCCCCCCC
RVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDVETSFMS
CCCCCEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEHHHHC
AGQVREIMEALARALWMEIKGVDLGDFPVMTFAEAMRRFGSDKPDLRNPLELVDVADLVK
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
SVDFKVFSGPANDARGRVIALRVPGGATLTRKNIDEYGQFVGIYGAKGLAWMKVNDRAAG
HCCCEEEECCCCCCCCCEEEEECCCCCEEECCCHHHHHCEEEEECCCCEEEEEECCCCCC
MDGVQSPIAKFLNAEVLEGILARSGAQSGDIIFFGADSAKVATDAMGALRLKVGRDLQLT
CCHHHHHHHHHHCHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHEEEECCCCEEEC
DESRWAPLWVVDFPMFEEDGEGGLAAMHHPFTSPRDISPEALKANPVGAIANAYDMVMNG
CCCCCCEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCHHHCCCCCHHHHHHHHHHHCC
YEVGGGSVRIHSGAMQSAVFDILGINEQEQREKFGFLLDALKFGTPPHAGLAFGLDRLVM
EEECCCEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
LLTGTENIRDVIAFPKTTAAACPLTDAPSRANPAALQELSIAVCAKQGSDA
HHCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHEEECCCCCC
>Mature Secondary Structure
MRTNYCGQLNLSHVGQEVTLCGWVHRRRDLGGLIFIDLRDREGVVQVFFDPDHQDAFRQA
CCCCCCCCCCHHHCCCHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEECCCHHHHHHHH
SELRNEFCVQVTGTVRARPESQRNSEMPTGEIEVFGHGLTLINRAEPLPLDFNQTNSEEN
HHHHHHEEEEEEEEEECCCCHHCCCCCCCCCEEEEECCEEEEECCCCCCCCCCCCCCHHH
RLKYRYLDLRRPEMAARLKTRAKITAFVRRFMDNHGFLDIETPMLTKATPEGARDYLVPS
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEECCCCCCCCCCCCCC
RVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDVETSFMS
CCCCCEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEHHHHC
AGQVREIMEALARALWMEIKGVDLGDFPVMTFAEAMRRFGSDKPDLRNPLELVDVADLVK
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
SVDFKVFSGPANDARGRVIALRVPGGATLTRKNIDEYGQFVGIYGAKGLAWMKVNDRAAG
HCCCEEEECCCCCCCCCEEEEECCCCCEEECCCHHHHHCEEEEECCCCEEEEEECCCCCC
MDGVQSPIAKFLNAEVLEGILARSGAQSGDIIFFGADSAKVATDAMGALRLKVGRDLQLT
CCHHHHHHHHHHCHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHEEEECCCCEEEC
DESRWAPLWVVDFPMFEEDGEGGLAAMHHPFTSPRDISPEALKANPVGAIANAYDMVMNG
CCCCCCEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCHHHCCCCCHHHHHHHHHHHCC
YEVGGGSVRIHSGAMQSAVFDILGINEQEQREKFGFLLDALKFGTPPHAGLAFGLDRLVM
EEECCCEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
LLTGTENIRDVIAFPKTTAAACPLTDAPSRANPAALQELSIAVCAKQGSDA
HHCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA