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The map label for this gene is prfA

Identifier: 238919470

GI number: 238919470

Start: 1525602

End: 1526684

Strand: Direct

Name: prfA

Synonym: NT01EI_1564

Alternate gene names: 238919470

Gene position: 1525602-1526684 (Clockwise)

Preceding gene: 238919469

Following gene: 238919471

Centisome position: 40.02

GC content: 63.53

Gene sequence:

>1083_bases
ATGAAGCCCTCTATCGTTGCCAAACTAGAAGCATTACAGGAGCGCCATGAAGAAGTTCAGGCACTGCTCGGTGATGCCGG
CGTCATTGCCGATCAGGACCGTTTTCGCGCGCTGTCGCGTGAGTACGCGCAGCTGACCGACGTATCGCACTGTTTTCTGG
CCTGGCGCCAGGTGCAGGACGATCTGACCACGGCGGAGATGCTGCTGGACGATCCGGAAATGCGGGATATGGCGCAGGAG
GAGCTCAAAGAGGCGCGCGGGCGCCTGGCCGAGCTGGAGCAGCAGTTGCAGATCCTGCTGCTGCCCAAAGATCCTGACGA
TGAGCGCGACTGTTTCCTTGAGGTGCGCGCCGGTACCGGCGGCGATGAGGCCGCGCTGTTTGCCGGCGATCTTTTCCGGA
TGTACAGCCGCTACGCTGAGGCACGTCGCTGGCGTATCGAGATCATGAGCGCCAGCGAGGGCGAACACGGCGGCTACAAA
GAGGTGATCGCCCGGGTGAGCGGTGACGGCGCCTATGGGCGTCTTAAGTTTGAGTCCGGCGGCCACCGCGTGCAGCGCGT
GCCGGCGACGGAGTCCCAGGGGCGTATTCACACCTCTGCCTGCACGGTGGCGGTGATGCCGGCGGTGCCGGAGGCCGAGC
TGCCGCAGATCAATCCGGCAGATCTGCGTATCGATACCTATCGCTCATCCGGTGCGGGCGGCCAGCACGTGAACACCACC
GACTCCGCCATTCGCATTACCCACCTGCCGACCGGGATCGTGGTGGAGTGTCAGGATGAGCGTTCACAGCATAAGAACAA
GGCCAAGGCCATGTCGGTGCTGGGCGCCCGTATTCGCGCCGCCGAAATCGCCAAGCGCCAGCAGGAGGAGGCATCGACCC
GCCGTAACCTGCTGGGCAGCGGCGATCGCTCTGATCGCGTCCGCACCTATAACTTCCCGCAGGGACGGGTGACCGACCAC
CGCATTAACCTGACCCTGTATCGTCTGGATGAGGTGATGGAGGGGAAGCTGGACAACCTGATTGAGCCCATCGTGCAGGA
GCACCAGGCCGATCAGCTCAGCGCGCTCGCCGAGCAGGAGTGA

Upstream 100 bases:

>100_bases
AGCGGCGATGCACAGCGTCTGCAGATGCTGCGCGACAGCCTTGGGCTGGATCATAATTAGTTCTCTCCTTTCTTATATAT
TTACAGGTAAATAGCCACGC

Downstream 100 bases:

>100_bases
TGCGCTACGATCAATGGTTGCGTCAGGCCTGCGCGCGCCTGACGCCGGGCGACAGCCCACGGCGCGATGCGGAGATCCTG
CTGGAGCATGTGACCGGCAG

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKPSIVAKLEALQERHEEVQALLGDAGVIADQDRFRALSREYAQLTDVSHCFLAWRQVQDDLTTAEMLLDDPEMRDMAQE
ELKEARGRLAELEQQLQILLLPKDPDDERDCFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRIEIMSASEGEHGGYK
EVIARVSGDGAYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPAVPEAELPQINPADLRIDTYRSSGAGGQHVNTT
DSAIRITHLPTGIVVECQDERSQHKNKAKAMSVLGARIRAAEIAKRQQEEASTRRNLLGSGDRSDRVRTYNFPQGRVTDH
RINLTLYRLDEVMEGKLDNLIEPIVQEHQADQLSALAEQE

Sequences:

>Translated_360_residues
MKPSIVAKLEALQERHEEVQALLGDAGVIADQDRFRALSREYAQLTDVSHCFLAWRQVQDDLTTAEMLLDDPEMRDMAQE
ELKEARGRLAELEQQLQILLLPKDPDDERDCFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRIEIMSASEGEHGGYK
EVIARVSGDGAYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPAVPEAELPQINPADLRIDTYRSSGAGGQHVNTT
DSAIRITHLPTGIVVECQDERSQHKNKAKAMSVLGARIRAAEIAKRQQEEASTRRNLLGSGDRSDRVRTYNFPQGRVTDH
RINLTLYRLDEVMEGKLDNLIEPIVQEHQADQLSALAEQE
>Mature_360_residues
MKPSIVAKLEALQERHEEVQALLGDAGVIADQDRFRALSREYAQLTDVSHCFLAWRQVQDDLTTAEMLLDDPEMRDMAQE
ELKEARGRLAELEQQLQILLLPKDPDDERDCFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRIEIMSASEGEHGGYK
EVIARVSGDGAYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPAVPEAELPQINPADLRIDTYRSSGAGGQHVNTT
DSAIRITHLPTGIVVECQDERSQHKNKAKAMSVLGARIRAAEIAKRQQEEASTRRNLLGSGDRSDRVRTYNFPQGRVTDH
RINLTLYRLDEVMEGKLDNLIEPIVQEHQADQLSALAEQE

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=288, Percent_Identity=49.3055555555556, Blast_Score=280, Evalue=2e-75,
Organism=Homo sapiens, GI34577120, Length=351, Percent_Identity=37.6068376068376, Blast_Score=235, Evalue=4e-62,
Organism=Homo sapiens, GI166795305, Length=193, Percent_Identity=50.259067357513, Blast_Score=192, Evalue=5e-49,
Organism=Escherichia coli, GI1787462, Length=360, Percent_Identity=87.5, Blast_Score=639, Evalue=0.0,
Organism=Escherichia coli, GI2367172, Length=336, Percent_Identity=41.0714285714286, Blast_Score=224, Evalue=5e-60,
Organism=Caenorhabditis elegans, GI17542784, Length=305, Percent_Identity=34.0983606557377, Blast_Score=187, Evalue=9e-48,
Organism=Saccharomyces cerevisiae, GI6321295, Length=329, Percent_Identity=41.3373860182371, Blast_Score=259, Evalue=4e-70,
Organism=Drosophila melanogaster, GI19921226, Length=327, Percent_Identity=39.7553516819572, Blast_Score=214, Evalue=5e-56,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_EDWI9 (C5B814)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002932985.1
- ProteinModelPortal:   C5B814
- GeneID:   7961045
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_1564
- OMA:   SEQGGYK
- ProtClustDB:   PRK00591
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.90.1150.10
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40314; Mature: 40314

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPSIVAKLEALQERHEEVQALLGDAGVIADQDRFRALSREYAQLTDVSHCFLAWRQVQD
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLTTAEMLLDDPEMRDMAQEELKEARGRLAELEQQLQILLLPKDPDDERDCFLEVRAGTG
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCEEEEEECCCC
GDEAALFAGDLFRMYSRYAEARRWRIEIMSASEGEHGGYKEVIARVSGDGAYGRLKFESG
CCCHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCHHHHHHHHCCCCCEEEEEECCC
GHRVQRVPATESQGRIHTSACTVAVMPAVPEAELPQINPADLRIDTYRSSGAGGQHVNTT
CCEEEECCCCCCCCEEEECCEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCC
DSAIRITHLPTGIVVECQDERSQHKNKAKAMSVLGARIRAAEIAKRQQEEASTRRNLLGS
CCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GDRSDRVRTYNFPQGRVTDHRINLTLYRLDEVMEGKLDNLIEPIVQEHQADQLSALAEQE
CCCCCCEEEEECCCCCCCCEEEEEEEEEHHHHHHCHHHHHHHHHHHHHCHHHHHHHHCCC
>Mature Secondary Structure
MKPSIVAKLEALQERHEEVQALLGDAGVIADQDRFRALSREYAQLTDVSHCFLAWRQVQD
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLTTAEMLLDDPEMRDMAQEELKEARGRLAELEQQLQILLLPKDPDDERDCFLEVRAGTG
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCEEEEEECCCC
GDEAALFAGDLFRMYSRYAEARRWRIEIMSASEGEHGGYKEVIARVSGDGAYGRLKFESG
CCCHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCHHHHHHHHCCCCCEEEEEECCC
GHRVQRVPATESQGRIHTSACTVAVMPAVPEAELPQINPADLRIDTYRSSGAGGQHVNTT
CCEEEECCCCCCCCEEEECCEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCC
DSAIRITHLPTGIVVECQDERSQHKNKAKAMSVLGARIRAAEIAKRQQEEASTRRNLLGS
CCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GDRSDRVRTYNFPQGRVTDHRINLTLYRLDEVMEGKLDNLIEPIVQEHQADQLSALAEQE
CCCCCCEEEEECCCCCCCCEEEEEEEEEHHHHHHCHHHHHHHHHHHHHCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA