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The map label for this gene is ispE [H]

Identifier: 238919467

GI number: 238919467

Start: 1522585

End: 1523463

Strand: Reverse

Name: ispE [H]

Synonym: NT01EI_1561

Alternate gene names: 238919467

Gene position: 1523463-1522585 (Counterclockwise)

Preceding gene: 238919468

Following gene: 238919466

Centisome position: 39.96

GC content: 59.84

Gene sequence:

>879_bases
ATGATCGACGCGCTGACCCACTGGCCGGCACCGGCCAAGCTGAATCTGTTTCTTTACATCACCGGGCGGCGGGCCGACGG
CTACCATCTGCTGCAGACCCTGTTCCAGTTTGTGGACTACGGCGACACGCTCACCATTCAGGCCCGCGGTGACGACCAGC
TGCGTCTGCTGACACCGGTGGCAGGAGTGGCGGATGAGGATAACCTGATCATCCGCGCGGCGCGGGCGCTGCGGGCGGCG
CTACCGCCCGCCTTTGCCGGATGCGGCGCCGACATCGCACTGCAAAAGATTCTGCCGATGGGGGGCGGGCTCGGCGGCGG
CTCATCGGATGCGGCCACCACCCTGGTCGCGCTGAATACCCTGTGGCAGGCCGGATTAAGCGATGCGCGCCTGGCCGAGA
TTGGATTGACCCTGGGCGCAGACGTACCTGTTTTTGTTAATGGCGTTGCCGCCTTTGCGCAGGGTGTTGGAGAATTGTTA
CAGACAGCACATCCTGAAGAAAAATGGTACTTTGTTGCACATCCAGGTATCAGCATTCCTACGCCAGTGATCTTCGCCGA
TCCTGCACTGAAAAGAGACTCTCCAATCAGAACTTTGACTGAACTGTTGGCAACACCATACAGTAATGATTGCGAGCCAA
TAGCAAGAAAACGTTTTCGTGAGGTTGAACAGGCGCTTTCGTGGCTGTTAGAATACGCGCCGTCGCGTCTGACAGGGACC
GGCGCCTGTGTGTTTGCGGAATTCGACACCGAGCAGGCGGCCCGTCAGGTGCTCAGCATTGCCCCCGAGTGGATGCAGGG
ATTCGTGGCGCGTGGCATCAATAGGTCGCCGCTGCACCATGCCCGCTCCGAGCTGCTGGCCTCTATAGCACGTAACTGA

Upstream 100 bases:

>100_bases
AGTATGACAGCGCCAGCGTCCCGGCCCTGCCGGCCCAGCTGGAGCTGACCCAGGGCGGCCAGCGGATCAAGCTGAAGATG
GACAGCTGGACGCTACGGCC

Downstream 100 bases:

>100_bases
TTAACGTTTAAAGCATTATACTGTGTTTGACAATAGCTTTACGTGCTACGCGGCCATTTGTTCGCCGAACGAATGCAGAG
TCTGCCGTGACTCAAACAAT

Product: 4-diphosphocytidyl-2C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase [H]

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MIDALTHWPAPAKLNLFLYITGRRADGYHLLQTLFQFVDYGDTLTIQARGDDQLRLLTPVAGVADEDNLIIRAARALRAA
LPPAFAGCGADIALQKILPMGGGLGGGSSDAATTLVALNTLWQAGLSDARLAEIGLTLGADVPVFVNGVAAFAQGVGELL
QTAHPEEKWYFVAHPGISIPTPVIFADPALKRDSPIRTLTELLATPYSNDCEPIARKRFREVEQALSWLLEYAPSRLTGT
GACVFAEFDTEQAARQVLSIAPEWMQGFVARGINRSPLHHARSELLASIARN

Sequences:

>Translated_292_residues
MIDALTHWPAPAKLNLFLYITGRRADGYHLLQTLFQFVDYGDTLTIQARGDDQLRLLTPVAGVADEDNLIIRAARALRAA
LPPAFAGCGADIALQKILPMGGGLGGGSSDAATTLVALNTLWQAGLSDARLAEIGLTLGADVPVFVNGVAAFAQGVGELL
QTAHPEEKWYFVAHPGISIPTPVIFADPALKRDSPIRTLTELLATPYSNDCEPIARKRFREVEQALSWLLEYAPSRLTGT
GACVFAEFDTEQAARQVLSIAPEWMQGFVARGINRSPLHHARSELLASIARN
>Mature_292_residues
MIDALTHWPAPAKLNLFLYITGRRADGYHLLQTLFQFVDYGDTLTIQARGDDQLRLLTPVAGVADEDNLIIRAARALRAA
LPPAFAGCGADIALQKILPMGGGLGGGSSDAATTLVALNTLWQAGLSDARLAEIGLTLGADVPVFVNGVAAFAQGVGELL
QTAHPEEKWYFVAHPGISIPTPVIFADPALKRDSPIRTLTELLATPYSNDCEPIARKRFREVEQALSWLLEYAPSRLTGT
GACVFAEFDTEQAARQVLSIAPEWMQGFVARGINRSPLHHARSELLASIARN

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol [H]

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787459, Length=282, Percent_Identity=70.2127659574468, Blast_Score=380, Evalue=1e-107,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721 [H]

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N [H]

EC number: =2.7.1.148 [H]

Molecular weight: Translated: 31450; Mature: 31450

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDALTHWPAPAKLNLFLYITGRRADGYHLLQTLFQFVDYGDTLTIQARGDDQLRLLTPV
CCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCEEEECCC
AGVADEDNLIIRAARALRAALPPAFAGCGADIALQKILPMGGGLGGGSSDAATTLVALNT
CCCCCCCCHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
LWQAGLSDARLAEIGLTLGADVPVFVNGVAAFAQGVGELLQTAHPEEKWYFVAHPGISIP
HHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCC
TPVIFADPALKRDSPIRTLTELLATPYSNDCEPIARKRFREVEQALSWLLEYAPSRLTGT
CCEEEECCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GACVFAEFDTEQAARQVLSIAPEWMQGFVARGINRSPLHHARSELLASIARN
CEEEEEECCHHHHHHHHHHHCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIDALTHWPAPAKLNLFLYITGRRADGYHLLQTLFQFVDYGDTLTIQARGDDQLRLLTPV
CCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCEEEECCC
AGVADEDNLIIRAARALRAALPPAFAGCGADIALQKILPMGGGLGGGSSDAATTLVALNT
CCCCCCCCHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
LWQAGLSDARLAEIGLTLGADVPVFVNGVAAFAQGVGELLQTAHPEEKWYFVAHPGISIP
HHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCC
TPVIFADPALKRDSPIRTLTELLATPYSNDCEPIARKRFREVEQALSWLLEYAPSRLTGT
CCEEEECCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GACVFAEFDTEQAARQVLSIAPEWMQGFVARGINRSPLHHARSELLASIARN
CEEEEEECCHHHHHHHHHHHCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA