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The map label for this gene is insA [H]

Identifier: 238919413

GI number: 238919413

Start: 1477031

End: 1477303

Strand: Direct

Name: insA [H]

Synonym: NT01EI_1507

Alternate gene names: 238919413

Gene position: 1477031-1477303 (Clockwise)

Preceding gene: 238919411

Following gene: 238919414

Centisome position: 38.74

GC content: 51.65

Gene sequence:

>273_bases
ATGGCCAGCGTTAACGTTCATTGTCCCCGTTATCAGTCTGCTCAGGTTTACCGCCATGATCAGAATCCTAAAGGCCATGA
CCGGTTTCGCTGCCGTGACTGCCACCGCGTCTTTCAGCTTACTTACGCCAATGAAGCCCGTAAACCGGGTATTAAAGAGC
AGATCACTGAAATGGCCTTCAAGGGTGCCGGGGGACGCGATACCGCCAGAACACTGAAATTTGCCATTAACACCGTCATC
CGGACGTTAAAAAACGCGCGCCAAAGCGAATAA

Upstream 100 bases:

>100_bases
AGTGATGTCGGAGATATCCAGCTTGCGGCAGACATCCGGTACGGCTAATTTCTCTAGTTGTTTGAATCTAATATATAATA
CTCGCTTTTGAGGTTCTTCC

Downstream 100 bases:

>100_bases
CGTCTTCACTTGTCGCCCATACTGATGTGGCGCTTATCTGCGAACTCAATGAGCAATGAAGCTTCGTGGACAATAAAGCC
CGGCAGCACTGGCTCTGGTA

Product: insertion element iso-IS1N protein InsA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 90; Mature: 89

Protein sequence:

>90_residues
MASVNVHCPRYQSAQVYRHDQNPKGHDRFRCRDCHRVFQLTYANEARKPGIKEQITEMAFKGAGGRDTARTLKFAINTVI
RTLKNARQSE

Sequences:

>Translated_90_residues
MASVNVHCPRYQSAQVYRHDQNPKGHDRFRCRDCHRVFQLTYANEARKPGIKEQITEMAFKGAGGRDTARTLKFAINTVI
RTLKNARQSE
>Mature_89_residues
ASVNVHCPRYQSAQVYRHDQNPKGHDRFRCRDCHRVFQLTYANEARKPGIKEQITEMAFKGAGGRDTARTLKFAINTVIR
TLKNARQSE

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG3677

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IS1 elements insA family [H]

Homologues:

Organism=Escherichia coli, GI1789852, Length=90, Percent_Identity=37.7777777777778, Blast_Score=75, Evalue=1e-15,
Organism=Escherichia coli, GI1788204, Length=90, Percent_Identity=37.7777777777778, Blast_Score=75, Evalue=1e-15,
Organism=Escherichia coli, GI1786204, Length=90, Percent_Identity=37.7777777777778, Blast_Score=75, Evalue=1e-15,
Organism=Escherichia coli, GI87081814, Length=90, Percent_Identity=37.7777777777778, Blast_Score=74, Evalue=3e-15,
Organism=Escherichia coli, GI1786471, Length=90, Percent_Identity=37.7777777777778, Blast_Score=74, Evalue=3e-15,
Organism=Escherichia coli, GI1786460, Length=90, Percent_Identity=37.7777777777778, Blast_Score=74, Evalue=3e-15,
Organism=Escherichia coli, GI1790747, Length=90, Percent_Identity=36.6666666666667, Blast_Score=73, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003220 [H]

Pfam domain/function: PF03811 Ins_element1 [H]

EC number: NA

Molecular weight: Translated: 10392; Mature: 10261

Theoretical pI: Translated: 10.66; Mature: 10.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASVNVHCPRYQSAQVYRHDQNPKGHDRFRCRDCHRVFQLTYANEARKPGIKEQITEMAF
CCCCCCCCCCCCCCHHEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
KGAGGRDTARTLKFAINTVIRTLKNARQSE
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ASVNVHCPRYQSAQVYRHDQNPKGHDRFRCRDCHRVFQLTYANEARKPGIKEQITEMAF
CCCCCCCCCCCCCHHEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
KGAGGRDTARTLKFAINTVIRTLKNARQSE
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6265806 [H]