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The map label for this gene is ecoRIIR [H]

Identifier: 238919388

GI number: 238919388

Start: 1455981

End: 1457225

Strand: Direct

Name: ecoRIIR [H]

Synonym: NT01EI_1482

Alternate gene names: 238919388

Gene position: 1455981-1457225 (Clockwise)

Preceding gene: 238919387

Following gene: 238919390

Centisome position: 38.19

GC content: 38.8

Gene sequence:

>1245_bases
ATGATATTGTTAATTCACTGGTTGTGTTTTATTTTAGGAGGTGAAATGTCTGACTTGTCTTCGTGGATAATTGATAAGTT
AACCGGCAATGCGTATGTGTATATAAAAAGATTGTCTGCCAATGATACCGGGGCTACCGGAGCACATCAGGTTGGAACTT
ATATGCAACACAGTGCGGCAGAGTATCTTTTTCCATCGATAAATAAGACTGATGAATGTAACCCATCTTTAATGATAACT
GCCAGAACATCATCACATTCATACCCGGAAAACCAAGTCAGAGTAATTTATTATAATAATAAGTTTTTTGGTAAAACCCG
GAATGAGAGAAGGATAACCCGTTGGGGAGGGGGAAGCCCCCTGCAAGATACCGAGAATACCGGAGCCTTGGCATTATTGG
TATTTGACTCGTCATTGGGAGGGAAGAGCGATTGTCAGTCTGTTGATGTTTGGGTATGTCGTGATGCTGATGAGGAGGAT
ATTGTTGAAGCCTCCATTGGGGAAATTCTGCCCGGAGATTTGATATTTGGTCTGGCTGATGAAGTTTTCGGCGGTTTTTT
TGCAAAGAGAACCTCATCCCATAAATACACCTTCCCGGATGAATGGAAAAAATATTTTCCATCAGGTAAAGAAATCATAT
CGTATGCGTCTGGTTATTACTCAAAGCATAATTTATCTCCTGACACTCAATTGCTGGAACGTCGAAAAATAGAGTATGAT
ATTTTTCTTCAGGTTGAGGAGTTACATGTTCTTAGTGTTATTAAAACTGGATTTTCAAGTGTTGATGCGTTTATATCACT
GGCAAACTCGGTTAGTAACAGGAGAAAGTCAAGATCTGGTAAATCACTTGAATTACATCTTGAAAACATTTTCTATGAAT
ATGGATTGACGGAGTTTGAAACTCAGGCTGTGACTGAAGGGAATAAAAAGCCAGATTTTTTATTCCCTTCAGCTAAGGCC
TATCATGATGATTCTTGGCCGCCACAAAAATTACGTATGCTGGCAGTAAAAACAACATGTAAGGATCGTTGGCGTCAGAT
ATTAAATGAAGCAGACAGGATCCAGGATATCTATTTGTTTACATTGCAGGAGGGGGTGTCTATTACTCAATTCCGTGAAA
TGCAGGAGGAGAGAGTTAGGCTTGTTGTTCCCGAGAAATTGCATGAAAAGTATCCTGCGGAAATACGTGGAAATTTAATT
TCATTGAATGATTTTATTTTTGATACTAAAAAACTGTACTCTTAA

Upstream 100 bases:

>100_bases
ATTTACGGTTTACTTTCTCTATCATTACATACCGTGATTGCTGTCATGATGACATAGTGAGTCTGTGATATGATACGGAA
TTATATTTTTTGATTAATGC

Downstream 100 bases:

>100_bases
AAATGATTGATTGAATACCATAAGTCTTCTGGTATTCAATCAAATATATTATTTATTATATTTTTCCTGCATGGCTTTAT
CTATATATGGAGCCAGTAGT

Product: type II restriction enzyme

Products: NA

Alternate protein names: R.EcoRII; Endonuclease EcoRII; Type II restriction enzyme EcoRII [H]

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MILLIHWLCFILGGEMSDLSSWIIDKLTGNAYVYIKRLSANDTGATGAHQVGTYMQHSAAEYLFPSINKTDECNPSLMIT
ARTSSHSYPENQVRVIYYNNKFFGKTRNERRITRWGGGSPLQDTENTGALALLVFDSSLGGKSDCQSVDVWVCRDADEED
IVEASIGEILPGDLIFGLADEVFGGFFAKRTSSHKYTFPDEWKKYFPSGKEIISYASGYYSKHNLSPDTQLLERRKIEYD
IFLQVEELHVLSVIKTGFSSVDAFISLANSVSNRRKSRSGKSLELHLENIFYEYGLTEFETQAVTEGNKKPDFLFPSAKA
YHDDSWPPQKLRMLAVKTTCKDRWRQILNEADRIQDIYLFTLQEGVSITQFREMQEERVRLVVPEKLHEKYPAEIRGNLI
SLNDFIFDTKKLYS

Sequences:

>Translated_414_residues
MILLIHWLCFILGGEMSDLSSWIIDKLTGNAYVYIKRLSANDTGATGAHQVGTYMQHSAAEYLFPSINKTDECNPSLMIT
ARTSSHSYPENQVRVIYYNNKFFGKTRNERRITRWGGGSPLQDTENTGALALLVFDSSLGGKSDCQSVDVWVCRDADEED
IVEASIGEILPGDLIFGLADEVFGGFFAKRTSSHKYTFPDEWKKYFPSGKEIISYASGYYSKHNLSPDTQLLERRKIEYD
IFLQVEELHVLSVIKTGFSSVDAFISLANSVSNRRKSRSGKSLELHLENIFYEYGLTEFETQAVTEGNKKPDFLFPSAKA
YHDDSWPPQKLRMLAVKTTCKDRWRQILNEADRIQDIYLFTLQEGVSITQFREMQEERVRLVVPEKLHEKYPAEIRGNLI
SLNDFIFDTKKLYS
>Mature_414_residues
MILLIHWLCFILGGEMSDLSSWIIDKLTGNAYVYIKRLSANDTGATGAHQVGTYMQHSAAEYLFPSINKTDECNPSLMIT
ARTSSHSYPENQVRVIYYNNKFFGKTRNERRITRWGGGSPLQDTENTGALALLVFDSSLGGKSDCQSVDVWVCRDADEED
IVEASIGEILPGDLIFGLADEVFGGFFAKRTSSHKYTFPDEWKKYFPSGKEIISYASGYYSKHNLSPDTQLLERRKIEYD
IFLQVEELHVLSVIKTGFSSVDAFISLANSVSNRRKSRSGKSLELHLENIFYEYGLTEFETQAVTEGNKKPDFLFPSAKA
YHDDSWPPQKLRMLAVKTTCKDRWRQILNEADRIQDIYLFTLQEGVSITQFREMQEERVRLVVPEKLHEKYPAEIRGNLI
SLNDFIFDTKKLYS

Specific function: Recognizes the double-stranded sequence CCWGG and cleaves before C-1 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011335
- InterPro:   IPR015109
- InterPro:   IPR015300 [H]

Pfam domain/function: PF09019 EcoRII-C; PF09217 EcoRII-N [H]

EC number: =3.1.21.4 [H]

Molecular weight: Translated: 47334; Mature: 47334

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILLIHWLCFILGGEMSDLSSWIIDKLTGNAYVYIKRLSANDTGATGAHQVGTYMQHSAA
CEEHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHH
EYLFPSINKTDECNPSLMITARTSSHSYPENQVRVIYYNNKFFGKTRNERRITRWGGGSP
HHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCEEECCCCCCCEEEECCCCCC
LQDTENTGALALLVFDSSLGGKSDCQSVDVWVCRDADEEDIVEASIGEILPGDLIFGLAD
CCCCCCCCEEEEEEEECCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHH
EVFGGFFAKRTSSHKYTFPDEWKKYFPSGKEIISYASGYYSKHNLSPDTQLLERRKIEYD
HHHHHHHHHCCCCCCCCCCHHHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCEE
IFLQVEELHVLSVIKTGFSSVDAFISLANSVSNRRKSRSGKSLELHLENIFYEYGLTEFE
EEEEEHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCHHH
TQAVTEGNKKPDFLFPSAKAYHDDSWPPQKLRMLAVKTTCKDRWRQILNEADRIQDIYLF
HHHHHCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEE
TLQEGVSITQFREMQEERVRLVVPEKLHEKYPAEIRGNLISLNDFIFDTKKLYS
EECCCCCHHHHHHHHHHHEEEECCHHHHHCCCHHHCCCEEEEHHHHHHHHHHCC
>Mature Secondary Structure
MILLIHWLCFILGGEMSDLSSWIIDKLTGNAYVYIKRLSANDTGATGAHQVGTYMQHSAA
CEEHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHH
EYLFPSINKTDECNPSLMITARTSSHSYPENQVRVIYYNNKFFGKTRNERRITRWGGGSP
HHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCEEECCCCCCCEEEECCCCCC
LQDTENTGALALLVFDSSLGGKSDCQSVDVWVCRDADEEDIVEASIGEILPGDLIFGLAD
CCCCCCCCEEEEEEEECCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHH
EVFGGFFAKRTSSHKYTFPDEWKKYFPSGKEIISYASGYYSKHNLSPDTQLLERRKIEYD
HHHHHHHHHCCCCCCCCCCHHHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCEE
IFLQVEELHVLSVIKTGFSSVDAFISLANSVSNRRKSRSGKSLELHLENIFYEYGLTEFE
EEEEEHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCHHH
TQAVTEGNKKPDFLFPSAKAYHDDSWPPQKLRMLAVKTTCKDRWRQILNEADRIQDIYLF
HHHHHCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEE
TLQEGVSITQFREMQEERVRLVVPEKLHEKYPAEIRGNLISLNDFIFDTKKLYS
EECCCCCHHHHHHHHHHHEEEECCHHHHHCCCHHHCCCEEEEHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2104830; 2597679; 2612358; 8392701 [H]