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The map label for this gene is helD [H]

Identifier: 238919307

GI number: 238919307

Start: 1386484

End: 1388586

Strand: Direct

Name: helD [H]

Synonym: NT01EI_1397

Alternate gene names: 238919307

Gene position: 1386484-1388586 (Clockwise)

Preceding gene: 238919304

Following gene: 238919310

Centisome position: 36.37

GC content: 64.05

Gene sequence:

>2103_bases
ATGGGTAAACTGGTCGGTATGATGGAATCAACGCAGGTGGAAAACGGCATGGAACTTAGAGCCACCAAACTGGGGCAGCG
TCTGGCGCAGCATCCGTATAACCGGGTACGCCTGTTGAACGCCGGTATTGAGGTCAGCGGAGAGCGGCACCAGTACCTGA
TCCCGTTTAACCAACTGATGGCGCTGCGCTGCAAGCGGGGGCTGGTATGGGGTGAGCTGGAGTTTGAGCTGCCGGATCAG
CGGGTCGTCCGCCTGCATGGTACAGACTGGCAGGAGACACAGCTGTTTCATCAGCACCTGATGCATAGCTGGTGGGCATG
GAGCGGGGAGATGGCGCAGATCAGCGCCGGGGTGCTGCAGACCCAGTGCCAGGCGCTGCAGTTGCTGGCGCAGCGCGATG
GCTGGCTGACGCGCGCGGATCTGGCGTCCCTGGCACAGGAGATCACCCGTACGCTGCAGGCGCTGCCGCTGCCGGTGCAG
CGTCTGGAGGCGTTTCCTGCGTGCGCCCCGCTGTACCACGACTGTTTGACGTGGCGCGATGAGGGGGAACAGCACCGCCA
GCAGCATAACCGCCGCTGGAGTCAACGGGTGCTGAGCGAGCGGGCGGATTTTTTTGCCGATGTCATGCCGTCGCCGCTCA
ATGACAGCCAGGCGCTGGCGGTGGTCAACGGCGAACAGAATGTGCTGGTGCTGGCCGGCGCCGGCAGCGGTAAGACCAGC
GTCCTGCTGGCGCGCTGCGCCTGGCTGCTGCAGGGCGGGCAGGTTGATGCCGGGCAGATATTGCCGCTGGCCTTTGGCCG
CCGTGCGGCGCAGGAGATGACGCAGCGCCTGCGGCAGACGCCAGGATGCGCAGAGGTTCAGGCCAGAACCTTTCATGGGC
TGGCGCTGTATATCGTTAATCAGACGGCTCGCAGACCGGTGGCTATTTCACGCCTGGCGAGCGATCGTCAGGCGCGCGAT
GCCTTTCTGCTCGCCGAGTGGCAGCGTCAATGTGCAGAGAAAAAAAGCCAGGCCAAAGGGTGGCGCCAATGGTTGGCGCT
GCTGTTCGACGATATTCCGGAGCGGGAGGACTTTTGGCAGGATGCTGCGTTGCAGGCGCGTCTGGCGCCCCGACTGGCGT
ACTGGGTCGGACTGCTGCGCGAGCACGGCGGCAGTCAGGCGGCGATGGTTGAACAGGCGTCGGAGGGGCTGCGGGCGCCG
TTTCAACAGCGCCTGCGCCTGCTGACACCACTGCTCAAGGCATGGAAGGCGGCGCTGAAGCAGGAGGGCGCGCTGGATTT
TCCGGCGCTGCTCCAACAGGCACAGGCGCAGCTGGAACAGGGGCGTTTCATCAGCCCGTGGCGACATATTCTGGTGGACG
AGTTTCAGGATATCTCGCCGCCGCGGGCACGACTTCTGGCCGCGCTGCGCAATCAGAACAGCCACAGCGCACTGTTTGCC
GTCGGAGATGACTGGCAGGCTATCTATCGTTTCAGTGGTGCCCAGCTAACGCTGACAACCGCCTTTCACCACTATTTTGG
TGAGGGAGAGTGCTGTCGGCTGGATACCTCTTACCGCTTTAACCGCCGTATCGGCGAGATCGCCAATGGGTTTGTACAGC
AGAATCCACACCAGGCGAAGAAGACGCTCAATGCCCTGCGCGATGGCGATCGGCAGTCGGTCACCCTGCTGCCGCAGGGC
CAGCTGGAAGCGCTGCTCGACAAGCTGAGCGGCTATGTGCGGGCTGATGAGTCCATTCTGCTGCTGGCGCGCTATCATCA
CCTGCGGCCAGCGCTGTTGGATCGTGCCGCGACGCGCTGGCCGGGGCTGGATCTCCGCTTTATGACCCTGCACGGCAGCA
AGGGGTGCCAGGCGGACTATGTGATCGTGCTGGGGCTACATGAGGGGCGAGATGGTTTCCCTGCACCGGAGGCGCCCAGC
GTGATCGAGGAGGTACTGCTGCCCGTGGCGGAGGCGTTCCCCGATGCCGAAGAGCGGCGGCTGCTGTATGTGGCGCTGAC
CCGCGCCCGCCATCGGGTATGGTTGCTACAGGATCCGCTGCGCCCCTCACGCTTTGTGGCCGCGCTGCAGCGCCTGGGCG
CTATCGCGCGACGTAAGGCGTAA

Upstream 100 bases:

>100_bases
TTTTGACCGCGTTGCTCCGGTGACAGGGTGCGCTGACGCCCCTCGCCTGCCACGGCACCGGATACAATTGTGGGAGCGTG
CCTAGAGGCGCCGTCGATTC

Downstream 100 bases:

>100_bases
TCTCCGCAGAGGCAGGGGTGGTGCCTGACGGCCCGCTCTGCACGCTGTATCAGATCAGGCGTTCGTTGAGATAGCGCTGA
TAGTCAGGAATGGCGATGCG

Product: DNA helicase IV

Products: NA

Alternate protein names: 75 kDa helicase [H]

Number of amino acids: Translated: 700; Mature: 699

Protein sequence:

>700_residues
MGKLVGMMESTQVENGMELRATKLGQRLAQHPYNRVRLLNAGIEVSGERHQYLIPFNQLMALRCKRGLVWGELEFELPDQ
RVVRLHGTDWQETQLFHQHLMHSWWAWSGEMAQISAGVLQTQCQALQLLAQRDGWLTRADLASLAQEITRTLQALPLPVQ
RLEAFPACAPLYHDCLTWRDEGEQHRQQHNRRWSQRVLSERADFFADVMPSPLNDSQALAVVNGEQNVLVLAGAGSGKTS
VLLARCAWLLQGGQVDAGQILPLAFGRRAAQEMTQRLRQTPGCAEVQARTFHGLALYIVNQTARRPVAISRLASDRQARD
AFLLAEWQRQCAEKKSQAKGWRQWLALLFDDIPEREDFWQDAALQARLAPRLAYWVGLLREHGGSQAAMVEQASEGLRAP
FQQRLRLLTPLLKAWKAALKQEGALDFPALLQQAQAQLEQGRFISPWRHILVDEFQDISPPRARLLAALRNQNSHSALFA
VGDDWQAIYRFSGAQLTLTTAFHHYFGEGECCRLDTSYRFNRRIGEIANGFVQQNPHQAKKTLNALRDGDRQSVTLLPQG
QLEALLDKLSGYVRADESILLLARYHHLRPALLDRAATRWPGLDLRFMTLHGSKGCQADYVIVLGLHEGRDGFPAPEAPS
VIEEVLLPVAEAFPDAEERRLLYVALTRARHRVWLLQDPLRPSRFVAALQRLGAIARRKA

Sequences:

>Translated_700_residues
MGKLVGMMESTQVENGMELRATKLGQRLAQHPYNRVRLLNAGIEVSGERHQYLIPFNQLMALRCKRGLVWGELEFELPDQ
RVVRLHGTDWQETQLFHQHLMHSWWAWSGEMAQISAGVLQTQCQALQLLAQRDGWLTRADLASLAQEITRTLQALPLPVQ
RLEAFPACAPLYHDCLTWRDEGEQHRQQHNRRWSQRVLSERADFFADVMPSPLNDSQALAVVNGEQNVLVLAGAGSGKTS
VLLARCAWLLQGGQVDAGQILPLAFGRRAAQEMTQRLRQTPGCAEVQARTFHGLALYIVNQTARRPVAISRLASDRQARD
AFLLAEWQRQCAEKKSQAKGWRQWLALLFDDIPEREDFWQDAALQARLAPRLAYWVGLLREHGGSQAAMVEQASEGLRAP
FQQRLRLLTPLLKAWKAALKQEGALDFPALLQQAQAQLEQGRFISPWRHILVDEFQDISPPRARLLAALRNQNSHSALFA
VGDDWQAIYRFSGAQLTLTTAFHHYFGEGECCRLDTSYRFNRRIGEIANGFVQQNPHQAKKTLNALRDGDRQSVTLLPQG
QLEALLDKLSGYVRADESILLLARYHHLRPALLDRAATRWPGLDLRFMTLHGSKGCQADYVIVLGLHEGRDGFPAPEAPS
VIEEVLLPVAEAFPDAEERRLLYVALTRARHRVWLLQDPLRPSRFVAALQRLGAIARRKA
>Mature_699_residues
GKLVGMMESTQVENGMELRATKLGQRLAQHPYNRVRLLNAGIEVSGERHQYLIPFNQLMALRCKRGLVWGELEFELPDQR
VVRLHGTDWQETQLFHQHLMHSWWAWSGEMAQISAGVLQTQCQALQLLAQRDGWLTRADLASLAQEITRTLQALPLPVQR
LEAFPACAPLYHDCLTWRDEGEQHRQQHNRRWSQRVLSERADFFADVMPSPLNDSQALAVVNGEQNVLVLAGAGSGKTSV
LLARCAWLLQGGQVDAGQILPLAFGRRAAQEMTQRLRQTPGCAEVQARTFHGLALYIVNQTARRPVAISRLASDRQARDA
FLLAEWQRQCAEKKSQAKGWRQWLALLFDDIPEREDFWQDAALQARLAPRLAYWVGLLREHGGSQAAMVEQASEGLRAPF
QQRLRLLTPLLKAWKAALKQEGALDFPALLQQAQAQLEQGRFISPWRHILVDEFQDISPPRARLLAALRNQNSHSALFAV
GDDWQAIYRFSGAQLTLTTAFHHYFGEGECCRLDTSYRFNRRIGEIANGFVQQNPHQAKKTLNALRDGDRQSVTLLPQGQ
LEALLDKLSGYVRADESILLLARYHHLRPALLDRAATRWPGLDLRFMTLHGSKGCQADYVIVLGLHEGRDGFPAPEAPSV
IEEVLLPVAEAFPDAEERRLLYVALTRARHRVWLLQDPLRPSRFVAALQRLGAIARRKA

Specific function: Helicase IV catalyzes the unwinding of duplex DNA in the 3' to 5' direction with respect to the bound single strand in a reaction that is dependent upon the hydrolysis of ATP [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase ATP-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787196, Length=687, Percent_Identity=60.5531295487627, Blast_Score=838, Evalue=0.0,
Organism=Escherichia coli, GI2367296, Length=344, Percent_Identity=30.2325581395349, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI48994965, Length=335, Percent_Identity=29.8507462686567, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000212
- InterPro:   IPR014016
- InterPro:   IPR022161
- InterPro:   IPR003661 [H]

Pfam domain/function: PF12462 Nucleolin_N; PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 79231; Mature: 79100

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKLVGMMESTQVENGMELRATKLGQRLAQHPYNRVRLLNAGIEVSGERHQYLIPFNQLM
CCCHHCCHHHHHHCCCHHHHHHHHHHHHHHCCHHHEEEEECCCEECCCCCEEECCHHHHH
ALRCKRGLVWGELEFELPDQRVVRLHGTDWQETQLFHQHLMHSWWAWSGEMAQISAGVLQ
HHHHHCCCEEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
TQCQALQLLAQRDGWLTRADLASLAQEITRTLQALPLPVQRLEAFPACAPLYHDCLTWRD
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHCC
EGEQHRQQHNRRWSQRVLSERADFFADVMPSPLNDSQALAVVNGEQNVLVLAGAGSGKTS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEEECCCCCHH
VLLARCAWLLQGGQVDAGQILPLAFGRRAAQEMTQRLRQTPGCAEVQARTFHGLALYIVN
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCEEEEEEC
QTARRPVAISRLASDRQARDAFLLAEWQRQCAEKKSQAKGWRQWLALLFDDIPEREDFWQ
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
DAALQARLAPRLAYWVGLLREHGGSQAAMVEQASEGLRAPFQQRLRLLTPLLKAWKAALK
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
QEGALDFPALLQQAQAQLEQGRFISPWRHILVDEFQDISPPRARLLAALRNQNSHSALFA
CCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEE
VGDDWQAIYRFSGAQLTLTTAFHHYFGEGECCRLDTSYRFNRRIGEIANGFVQQNPHQAK
ECCCHHHHHHCCCCEEEEEHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHCCHHHHH
KTLNALRDGDRQSVTLLPQGQLEALLDKLSGYVRADESILLLARYHHLRPALLDRAATRW
HHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHEECCCCEEEHHHHHHHHHHHHHHHHHCC
PGLDLRFMTLHGSKGCQADYVIVLGLHEGRDGFPAPEAPSVIEEVLLPVAEAFPDAEERR
CCCCEEEEEEECCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHCE
LLYVALTRARHRVWLLQDPLRPSRFVAALQRLGAIARRKA
EEEEEEHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GKLVGMMESTQVENGMELRATKLGQRLAQHPYNRVRLLNAGIEVSGERHQYLIPFNQLM
CCHHCCHHHHHHCCCHHHHHHHHHHHHHHCCHHHEEEEECCCEECCCCCEEECCHHHHH
ALRCKRGLVWGELEFELPDQRVVRLHGTDWQETQLFHQHLMHSWWAWSGEMAQISAGVLQ
HHHHHCCCEEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
TQCQALQLLAQRDGWLTRADLASLAQEITRTLQALPLPVQRLEAFPACAPLYHDCLTWRD
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHCC
EGEQHRQQHNRRWSQRVLSERADFFADVMPSPLNDSQALAVVNGEQNVLVLAGAGSGKTS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEEECCCCCHH
VLLARCAWLLQGGQVDAGQILPLAFGRRAAQEMTQRLRQTPGCAEVQARTFHGLALYIVN
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCEEEEEEC
QTARRPVAISRLASDRQARDAFLLAEWQRQCAEKKSQAKGWRQWLALLFDDIPEREDFWQ
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
DAALQARLAPRLAYWVGLLREHGGSQAAMVEQASEGLRAPFQQRLRLLTPLLKAWKAALK
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
QEGALDFPALLQQAQAQLEQGRFISPWRHILVDEFQDISPPRARLLAALRNQNSHSALFA
CCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEE
VGDDWQAIYRFSGAQLTLTTAFHHYFGEGECCRLDTSYRFNRRIGEIANGFVQQNPHQAK
ECCCHHHHHHCCCCEEEEEHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHCCHHHHH
KTLNALRDGDRQSVTLLPQGQLEALLDKLSGYVRADESILLLARYHHLRPALLDRAATRW
HHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHEECCCCEEEHHHHHHHHHHHHHHHHHCC
PGLDLRFMTLHGSKGCQADYVIVLGLHEGRDGFPAPEAPSVIEEVLLPVAEAFPDAEERR
CCCCEEEEEEECCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHCE
LLYVALTRARHRVWLLQDPLRPSRFVAALQRLGAIARRKA
EEEEEEHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2542273; 8905232; 9278503 [H]